Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,872,673 |
C→A |
33.1% |
N432K (AAC→AAA) |
ygcE → |
predicted kinase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,872,673 | 0 | C | A | 33.1%
| 135.2
/ 73.9
| 116 | N432K (AAC→AAA) | ygcE | predicted kinase |
| Reads supporting (aligned to +/- strand): ref base C (20/56); new base A (31/7); total (52/64) |
| Fisher's exact test for biased strand distribution p-value = 2.33e-08 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01 |
Only 100 of 116 total aligned reads displayed.
GGTAGGTCTGGGGCTATACCCGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCAATGCCAAACGCTACGACGCGATGAAT > minE/1872628‑1872739
|
ggTAGGTCTGGGGCTATACCCGGATTACGCAACGGCTGTCGATAAGATGGTTCGCGTGAAAGATATCtt < 1:2001960/69‑1 (MQ=255)
ggTAGGTCTGGGGCTATACCCGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCtt < 1:1014479/69‑1 (MQ=255)
ggTAGGTCTGGGGCTATACCCGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCtt < 1:271771/69‑1 (MQ=255)
ggTAGGTCTGGGGCTATACCCGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCtt < 1:2377137/69‑1 (MQ=255)
ggTAGGTCTGGGGCTATACCCGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCtt < 1:1180047/69‑1 (MQ=255)
ggTAGGTCTGGGGCTATACCCGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCtt < 1:926472/69‑1 (MQ=255)
ggTAGGTCTGGGGCTATACCCGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCtt < 1:356783/69‑1 (MQ=255)
ggTAGGTCTGGGGCTATACCCGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCtt < 1:2260819/69‑1 (MQ=255)
ggTAGGTCTGGGGCTATACCCGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCtt < 1:2026466/69‑1 (MQ=255)
ggTAGGTCTGGGGCTATACCCGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCtt < 1:1981500/69‑1 (MQ=255)
ggTAGGTCTGGGGCTATACCCGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCtt < 1:470986/69‑1 (MQ=255)
ggTAGGTCTGGGGCTATACCCGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCtt < 1:1667164/69‑1 (MQ=255)
ggTAGGTCTGGGGCTATACCCGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCtt < 1:1634574/69‑1 (MQ=255)
ggTAGGTCTGGGGCTATACCCGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCtt < 1:1519356/69‑1 (MQ=255)
ggTAGGTCTGGGGCTATACCCGGATTACGCAACGGCTGTCGATAACATGGATCGCGTGAAAGATATCtt < 1:2061144/69‑1 (MQ=255)
gTAGGTCTGGGGCTATACCCGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCtt < 1:2844952/68‑1 (MQ=255)
ggTCTGGGGCTATACCCGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTtata > 1:2147206/1‑69 (MQ=255)
ggTCTGGGGCTATACCCGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTtata > 1:908499/1‑69 (MQ=255)
ggTCTGGGGCTATACCCGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTtata > 1:1947710/1‑69 (MQ=255)
tCTGGGGCTATACCCGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATAcc < 1:1783625/69‑1 (MQ=255)
tGGGGCTATACCCGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGa < 1:301596/69‑1 (MQ=255)
tGGGGCTATACCCGGATTACACAACGGCTGTCGATAAAATGGTTCGCGTGAAAGATATCTTCATACCg > 1:2233681/1‑68 (MQ=255)
tGGGGCTATACCCGGATTACACAACGGCTGTCGATAAAATGGTTCGCGTGAAAGATATCTTCATACCg > 1:230725/1‑68 (MQ=255)
tGGGGCTATACCCGGATTACACAACGGCTGTCGATAAAATGGTTCGCGTGAAAGATATCTTCATACCGa > 1:1663494/1‑69 (MQ=255)
tGGGGCTATACCCGGATTACACAACGGCTGTCGATAAAATGGTTCGCGTGAAAGATATCTTCATACCGa > 1:3238365/1‑69 (MQ=255)
tGGGGCTATACCCGGATTACACAACGGCTGTCGATAAAATGGTTCGCGTGAAAGATATCTTCATACCGa > 1:62038/1‑69 (MQ=255)
tGGGGCTATACCCGGATTACACAACGGCTGTCGATAAAATGGTTCGCGTGAAAGATATCTTCATACCGa > 1:3326747/1‑69 (MQ=255)
tGGGGCTATACCCGGATTACACAACGGCTGTCGATAAAATGGTTCGCGTGAAAGATATCTTCATACCGa > 1:3371099/1‑69 (MQ=255)
tGGGGCTATACCCGGATTACACAACGGCTGTCGATAAAATGGTTCGCGTGAAAGATATCTTCATACCGa > 1:847866/1‑69 (MQ=255)
tGGGGCTATACCCGGATTACACAACGGCTGTCGATAAAATGGTTCGCGTGAAAGATATCTTCATACCGa > 1:2553891/1‑69 (MQ=255)
tGGGGCTATACCCGGATTACACAACGGCTGTCGATAAAATGGTTCGCGTGAAAGATATCTTCATACCGa > 1:1335231/1‑69 (MQ=255)
tGGGGCTATACCCGCATTACACAACGGCTGTCGATAATATGGTTCGCGTGAAAGATATCTTCATACCGa > 1:2431865/1‑69 (MQ=255)
tGGGGCGATACCCGGATTACACAACGGCTGTCGATAAAATGGTTCGCGTGAAAGATATCTTCATACCGa > 1:3020/1‑69 (MQ=255)
gggCTATACCCGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGa > 1:2714913/1‑67 (MQ=255)
ataCCCGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTgaga > 1:1582180/1‑68 (MQ=255)
taCCCGGATTACGCAACGGCTGTCGATAACATGGTTCGGGTGAAAGATATCTTTATACCGATTGAGAGc < 1:2464983/69‑1 (MQ=255)
cccGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGc < 1:1512264/67‑1 (MQ=255)
cccGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGc < 1:363335/67‑1 (MQ=255)
cccGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGc < 1:3371270/67‑1 (MQ=255)
cccGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGc < 1:2655540/67‑1 (MQ=255)
cccGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGc < 1:3327540/67‑1 (MQ=255)
cccGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGc < 1:1143153/67‑1 (MQ=255)
cccGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGc < 1:2761953/67‑1 (MQ=255)
cccGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGc < 1:2844417/67‑1 (MQ=255)
cccGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGc < 1:168967/67‑1 (MQ=255)
cccGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGc < 1:2139977/67‑1 (MQ=255)
cccGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGc < 1:388596/67‑1 (MQ=255)
cccGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGc < 1:726139/67‑1 (MQ=255)
cccGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGc < 1:1425078/67‑1 (MQ=255)
cccGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCaa > 1:3381351/1‑69 (MQ=255)
cccGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCaa > 1:1360916/1‑69 (MQ=255)
cccGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCaa > 1:268767/1‑69 (MQ=255)
cccGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCaa > 1:2826826/1‑69 (MQ=255)
cccGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCaa > 1:1524093/1‑69 (MQ=255)
cccGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCaa > 1:1481573/1‑69 (MQ=255)
cccGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCaa > 1:2086497/1‑69 (MQ=255)
cccGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCaa > 1:3274752/1‑69 (MQ=255)
cccGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCaa > 1:221201/1‑69 (MQ=255)
cccGGATTACGCAACGGCTGTCGATAACATGGATCGCGTGAAAGATATCTTTATACCGATTGAGAGCaa > 1:2132856/1‑69 (MQ=255)
ccaggATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCaa > 1:2277287/4‑69 (MQ=255)
cacGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGc < 1:931381/65‑1 (MQ=255)
cGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCAATg < 1:2248126/69‑1 (MQ=255)
cGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCAATg < 1:1616353/69‑1 (MQ=255)
cGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCAATg < 1:1395448/69‑1 (MQ=255)
cGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCAATg < 1:434827/69‑1 (MQ=255)
cGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCAATg < 1:1833466/69‑1 (MQ=255)
cGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCAATg < 1:2583056/69‑1 (MQ=255)
cGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCAATg < 1:200735/69‑1 (MQ=255)
cGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCAATg < 1:1271559/69‑1 (MQ=255)
aCGGCTGTCGATAAAATGGTTCGCGTGAAATATATCTTCATACCGATTGAGAGCAATGCCAAACGCTac < 1:1996874/69‑1 (MQ=255)
aCGGCTGTCGATAAAATGGTTCGCGTGAAAGATATCTTCATACCGATTGAGAGCAATGCCAAACGCTac < 1:267360/69‑1 (MQ=255)
aCGGCTGTCGATAAAATGGTTCGCGTGAAAGATATCTTCATACCGATTGAGAGCAATGCCAAACGCTac < 1:1354842/69‑1 (MQ=255)
aCGGCTGTCGATAAAATGGTTCGCGTGAAAGATATCTTCATACCGATTGAGAGCAATGCCAAACGCTac < 1:2418151/69‑1 (MQ=255)
aCGGCTGTCGATAAAATGGTTCGCGTGAAAGATATCTTCATACCGATTGAGAGCAATGCCAAACGCTac < 1:3087565/69‑1 (MQ=255)
aCGGCTGTCGATAAAATGGTTCGCGTGAAAGATATCTTCATACCGATTGAGAGCAATGCCAAACGCTac < 1:1481915/69‑1 (MQ=255)
ggCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCAATGCCAAACGCTacga < 1:1059939/69‑1 (MQ=255)
ggCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCAATGCCAAACGCTacga < 1:2289315/69‑1 (MQ=255)
gTCGATAAAATGGTTCGCGTGAAAGATATCTTCATACCGATTGAGAGCAATGCCAAACGCt > 1:1707379/1‑61 (MQ=255)
gTCGATAAAATGGTTCGCGTGAAAGATATCTTCATACCGATTGAGAGCAATGCCAAACGCTACGActcg > 1:2638911/1‑69 (MQ=255)
gTCGATAAAATGGTTCGCGTGAAAGATATCTTCATACCGATTGAGAGCAATGCCAAACGCTACGAcgcg > 1:3016647/1‑69 (MQ=255)
gTCGATAAAATGGTTCGCGTGAAAGATATCTTCATACCGATTGAGAGCAATGCCAAACGCTACGAcgcg > 1:1248946/1‑69 (MQ=255)
gTCGATAAAATGGTTCGCGTGAAAGATATCTTCATACCGATTGAGAGCAATGCCAAACGCTACGAcgcg > 1:869596/1‑69 (MQ=255)
gTCGATAAAATGGTTCGCGTGAAAGATATCTTCATACCGATTGAGAGCAATGCCAAACGCTACGAcgcg > 1:857445/1‑69 (MQ=255)
gTCGATAAAATGGTTCGCGTGAAAGATATCTTCATACCGATTGAGAGCAATGCCAAACGCTACGAcgcg > 1:164707/1‑69 (MQ=255)
gTCGATAAAATGGTTCGCGTGAAAGATATCTTCATACCGATTGAGAGCAATGCCAAACGCTACGAcgcg > 1:636503/1‑69 (MQ=255)
gTCGATAAAATGGTTCGCGTGAAAGATATCTTCATACCGATTGAGAGCAATGCCAAACGCTACGAcgcg > 1:632220/1‑69 (MQ=255)
gTCGATAAAATGGTTCGCGTGAAAGATATCTTCATACCGATTGAGAGCAATGCCAAACGCTACGAcgcg > 1:1703275/1‑69 (MQ=255)
gTCGATAAAATGGTTCGCGTGAAAGATATCTTCATACCGATTGAGAGCAATGCCAAACGCTACGAcgcg > 1:523043/1‑69 (MQ=255)
gTCGATAAAATGGTTCGCGTGAAAGATATCTTCATACCGATTGAGAGCAATGCCAAACGCTACGAcgcg > 1:429925/1‑69 (MQ=255)
gTCGATAAAATGGTTCGCGTGAAAGATATCTTCATACCGATTGAGAGCAATGCCAAACGCTACGAcgcg > 1:429752/1‑69 (MQ=255)
gTCGATAAAATGGTTCGCGTGAAAGATATCTTCATACCGATTGAGAGCAATGCCAAACGCTACGAcgcg > 1:1894122/1‑69 (MQ=255)
gTCGATAAAATGGTTCGCGTGAAAGATATCTTCATACCGATTGAGAGCAATGCCAAACGCTACGAcgcg > 1:2365126/1‑69 (MQ=255)
gTCGATAAAATGGTTCGCGTGAAAGATATCTTCATACCGATTGAGAGCAATGCCAAACGCTACGAcgcg > 1:243214/1‑69 (MQ=255)
gTCGATAAAATGGTTCGCGTGAAAGATATCTTCATACCGATTGAGAGCAATGCCAAACGCTACGAcgcg > 1:2509417/1‑69 (MQ=255)
gTCGATAAAATGGTTCGCGTGAAAGATATCTTCATACCGATTGAGAGCAATGCCAAACGCTACGAcgcg > 1:2990893/1‑69 (MQ=255)
gTCAATAAAATGGTTCGCGTGAAAGATATCTTCATACCGATTGAGAGCAATGCCAAACGCTACGAcgcg > 1:122519/1‑69 (MQ=255)
aaCATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCAATGCCAAACGCTACGACGCGATGAAt < 1:177963/69‑1 (MQ=255)
aaCATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCAATGCCAAACGCTACGACGCGATGAAt < 1:1358426/69‑1 (MQ=255)
aaCATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCAATGCCAAACGCTACGACGCGATGAAt < 1:3074233/69‑1 (MQ=255)
aaCATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCAATGCCAAACGCTACGACGCGATGAAt < 1:1151296/69‑1 (MQ=255)
|
GGTAGGTCTGGGGCTATACCCGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCAATGCCAAACGCTACGACGCGATGAAT > minE/1872628‑1872739
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
