Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,922,014 |
T→C |
47.5% |
V346V (GTA→GTG) |
aas ← |
fused 2‑acylglycerophospho‑ethanolamine acyl transferase and acyl‑acyl carrier protein synthetase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,922,014 | 0 | T | C | 47.5%
| 0.9
/ 70.9
| 80 | V346V (GTA→GTG) | aas | fused 2‑acylglycerophospho‑ethanolamine acyl transferase and acyl‑acyl carrier protein synthetase |
| Reads supporting (aligned to +/- strand): ref base T (17/25); new base C (18/20); total (35/45) |
| Fisher's exact test for biased strand distribution p-value = 6.53e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TCTTCTTCCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGATCCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTG > minE/1921952‑1922082
|
tcttctTCCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGATCCATACTTTg > 1:961611/1‑69 (MQ=255)
tcttctTCCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGATCCATACTTTg > 1:2090620/1‑69 (MQ=255)
tcttctTCCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGATCCATACTTTg > 1:240643/1‑69 (MQ=255)
tcttctTCCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGATCCATACTTTg > 1:2571451/1‑69 (MQ=255)
tcttctTCCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGATCCATACTTTg > 1:3062783/1‑69 (MQ=255)
tcCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGATCCATACTTTGTCGGc < 1:2265824/68‑1 (MQ=255)
tcCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGATCCATACTTTGTCGGc < 1:1966150/68‑1 (MQ=255)
tcCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGATCCATACTTTGTCGGc < 1:196201/68‑1 (MQ=255)
tcCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGATCCATACTTTGTCGGc < 1:1944222/68‑1 (MQ=255)
tcCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGATCCATACTTTGTCGGc < 1:1738195/68‑1 (MQ=255)
tcCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGATCCATACTTTGTCGGc < 1:951128/68‑1 (MQ=255)
tcCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGATCCATACTTTGTCGGc < 1:1131145/68‑1 (MQ=255)
tcCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGATCCATACTTTGTCGGc < 1:1167873/68‑1 (MQ=255)
ggCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGATCCATACTTTGTCGGCAg < 1:322878/67‑1 (MQ=255)
ggCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGATCCATACTTTGTCGGCAg < 1:2033239/67‑1 (MQ=255)
ggCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGATCCATACTTTGTCGGCAg < 1:3007996/67‑1 (MQ=255)
ggCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGATCCATACTTTGTCGGCAg < 1:516344/67‑1 (MQ=255)
tgctgTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGATCCATACTTTGTCGGCAgtggtg < 1:2605232/69‑1 (MQ=255)
tgctgTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGATCCATACTTTGTCGGCAgtggtg < 1:2780019/69‑1 (MQ=255)
tgctgTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGATCCATACTTTGTCGGCAgtggtg < 1:2863826/69‑1 (MQ=255)
gTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGATCCATACTTTGTCGGCAGTGGTGAc < 1:1833121/67‑1 (MQ=255)
gTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGATCCATACTTTGTCGGCAGTGGTGAc < 1:1709060/67‑1 (MQ=255)
gTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGATCCATACTTTGTCGGCAGTGGTGAc < 1:3021060/67‑1 (MQ=255)
gTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGATCCATACTTTGTCGGCAGTGGTGAc < 1:2346460/67‑1 (MQ=255)
gTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGATCCATACTTTGTCCGCAGTGGTGAc < 1:1572367/67‑1 (MQ=255)
tgtgCCAGACGCGGCATCAGCAAATGAGCGAAAATCCACACTTTGTCGGCAGTGGTGACATCTGCttt < 1:2790984/68‑1 (MQ=255)
tgtgCCAGACGCGGCATCAGCAAATGAGCGAAAATCCACACTTTGTCGGCAGTGGTGACATCTGCttt < 1:3387402/68‑1 (MQ=255)
tgtgCCAGACGCGGCATCAGCAAATGAGCGAAAATCCACACTTTGTCGGCAGTGGTGACATCTGCttt < 1:3123314/68‑1 (MQ=255)
tgtgCCAGACGCGGCATCAGCAAATGAGCGAAAATCCACACTTTGTCGGCAGTGGTGACATCTGCttt < 1:3394646/68‑1 (MQ=255)
tgtgCCAGACGCGGCATCAGCAAATGAGCGAAAATCCACACTTTGTCGGCAGTGGTGACATCTGCttt < 1:2438258/68‑1 (MQ=255)
tgtgCCAGACGCGGCATCAGCAAATGAGCGAAAATCCACACTTTGTCGGCAGTGGTGACATCTGCttt < 1:54234/68‑1 (MQ=255)
tgtgCCAGACGCGGCATCAGCAAATGAGCGAAAATCCACACTTTGTCGGCAGTGGTGACATCTGCttt < 1:2996680/68‑1 (MQ=255)
tgtgCCAGACGCGGCATCAGCAAATGAGCGAAAATCCACACTTTGTCGGCAGTGGTGACATCTGCttt < 1:2340278/68‑1 (MQ=255)
tgtgCCAGACGCGGCATCAGCAAATGAGCGAAAATCCACACTTTGTCGGCAGTGGTGACATCTGCttt < 1:1113880/68‑1 (MQ=255)
tgtgCCAGACGCGGCATCAGCAAATGAGCGAAAATCCACACTTTGTCGGCAGTGGTGACATCTGCttt < 1:1519917/68‑1 (MQ=255)
tgtgCCAGACGCGGCATCAGCAAATGAGCGAAAATCCACACTTTGTCGGCAGTGGTGACATCTGCttt < 1:1125812/68‑1 (MQ=255)
tgtgCCAGACGCGGCATCAGCAAATGAGCGAAAATCCACACTTTGTCGGCAGTGGTGACATCTGCttt < 1:2148382/68‑1 (MQ=255)
tgtgCCAGACGCGGCATCAGCAAATGAGCGAAAATCCACACTTTGTCGGCAGTGGTGACATCTGCttt < 1:2001319/68‑1 (MQ=255)
tgtgCCAGACGCGGCATCAGCAAATGAGCGAAAATCCACACTTTGTCGGCAGTGGTGACATCTGCttt < 1:1851864/68‑1 (MQ=255)
tgtgCCAGACGCGGCATCAGCAAATGAGCGAAAATCCACACTTTGTCGGCAGTGGTGACATCTGCttt < 1:16282/68‑1 (MQ=255)
tgtgCCAGACGCGGCATCAGCAAATGAGCGAAAATCCACACTTTGTCGGCAGTGGTGACATCTGCttt < 1:1775732/68‑1 (MQ=255)
tgtgCCAGACGCGGCATCAGCAAATGAGCGAAAATCCACACTTTGTCGGCAGTGGTGACATCTGCttt < 1:1370503/68‑1 (MQ=255)
tgtgCCAGACGCGGCATCAGCAAATGAGCCAAAATCCACACTTTGTCGGCAGTGGTGACATCTGCttt < 1:3325842/68‑1 (MQ=255)
gCCAGACGCGGCATCAGCAAATGAGCGAAAATCCACACTTTGTCGGCAGTGGTGACATCTGCttt < 1:3169962/65‑1 (MQ=255)
aTCAGCAAATGAGCGAAGATCCATACTTTGTCGGc < 1:1897852/35‑1 (MQ=255)
aGCAAATGAGCGAAGATCCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGa > 1:1617894/1‑67 (MQ=255)
aGCAAATGAGCGAAGATACATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGa > 1:2765630/1‑67 (MQ=255)
cAAATGAGCGAAGATCCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGAccc < 1:1196408/68‑1 (MQ=255)
cAAATGAGCGAAGATCCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCa > 1:3428748/1‑69 (MQ=255)
cAAATGAGCGAAGATCCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCa > 1:53961/1‑69 (MQ=255)
cAAATGAGCGAAGATCCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCa > 1:113624/1‑69 (MQ=255)
cAAATGAGCGAAGATCCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCa > 1:584864/1‑69 (MQ=255)
cAAATGAGCGAAGATCCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCa > 1:3011397/1‑69 (MQ=255)
cAAATGAGCGAAGATCCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCa > 1:779047/1‑69 (MQ=255)
cAAATGAGCGAAGATCCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCa > 1:3143385/1‑69 (MQ=255)
cAAATGAGCGAAGATCCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCa > 1:2268473/1‑69 (MQ=255)
cAAATGAGCGAAGATCCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTT‑‑AGATAGACCCAg > 1:1271105/1‑68 (MQ=255)
cAAATGAGCGAAGATCAATACTTTGTCGGCAgaggt > 1:1400882/1‑36 (MQ=37)
aaTGAGCGAAAATCCACACTTTGTCGGCAGTGGTGACATCTGCttt < 1:446937/46‑1 (MQ=255)
gAGCGAAGATCCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCa < 1:110576/69‑1 (MQ=255)
gAGCGAAGATCCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCa < 1:3280989/69‑1 (MQ=255)
gAGCGAAGATCCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCa < 1:2348710/69‑1 (MQ=255)
aaaTCCACACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATa > 1:1730825/3‑52 (MQ=255)
aaaTCCACACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGTACGTGa > 1:1452889/3‑68 (MQ=255)
aaaTCCACACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGTACCTgg > 1:2445521/3‑67 (MQ=255)
aaaTCCACACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGTACCTg > 1:2976559/3‑67 (MQ=255)
aaaTCCACACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGTACCTGa > 1:2249970/3‑68 (MQ=255)
aaaTCCACACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGTACCTGa > 1:3104221/3‑68 (MQ=255)
aaaTCCACACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGTACCTGa > 1:2177908/3‑68 (MQ=255)
aaaTCCACACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGTACCTGa > 1:1850403/3‑68 (MQ=255)
aaaTCCACACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGTACCTGa > 1:2822494/3‑68 (MQ=255)
aaaTCCACACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGTACCTGa > 1:477005/3‑68 (MQ=255)
aaaTCCACACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGTACCTGa > 1:2909605/3‑68 (MQ=255)
aaaTCCACACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGTACCTGa > 1:2915560/3‑68 (MQ=255)
aaaTCCACACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGTACCTGa > 1:2978279/3‑68 (MQ=255)
aaaTCCACACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGTACCTGa > 1:3250185/3‑68 (MQ=255)
aaaTCCACACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGTACCTGa > 1:3245813/3‑68 (MQ=255)
aaaTCCACACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGTACCTGa > 1:1592098/3‑68 (MQ=255)
aaaTCCACACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGTACCTGa > 1:3027424/3‑68 (MQ=255)
aaaTCCACACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGTACCTGa > 1:3054269/3‑68 (MQ=255)
tACTTTGTCGGCAGTGGTGACATCTGCTTTTGAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTg > 1:1786459/1‑69 (MQ=255)
tACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTg > 1:2892896/1‑69 (MQ=255)
|
TCTTCTTCCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGATCCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTG > minE/1921952‑1922082
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
