Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,940,739 |
A→T |
34.3% |
A53A (GCA→GCT) ‡ |
ygfZ → |
predicted folate‑dependent regulatory protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,940,739 | 0 | A | T | 34.3%
| 66.5
/ 52.7
| 70 | A53A (GCA→GCT) ‡ | ygfZ | predicted folate‑dependent regulatory protein |
| Reads supporting (aligned to +/- strand): ref base A (24/22); new base T (18/6); total (42/28) |
| Fisher's exact test for biased strand distribution p-value = 7.68e-02 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTA > minE/1940675‑1940799
|
ttACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCagaag < 1:1162579/69‑1 (MQ=255)
ggCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAg < 1:2672796/69‑1 (MQ=255)
ggCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAg < 1:116570/69‑1 (MQ=255)
ggCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAg < 1:177899/69‑1 (MQ=255)
ggCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAg < 1:1258583/69‑1 (MQ=255)
ggCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAg < 1:3275531/69‑1 (MQ=255)
ggCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAg < 1:2191651/69‑1 (MQ=255)
ggCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAg < 1:1396840/69‑1 (MQ=255)
ggCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAg < 1:557450/69‑1 (MQ=255)
ggCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAg < 1:497588/69‑1 (MQ=255)
gcgcGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAg < 1:1554521/68‑1 (MQ=255)
gcgGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCa < 1:2703698/68‑1 (MQ=255)
gcgGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCa < 1:2853765/68‑1 (MQ=255)
gcgGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCa < 1:1964884/68‑1 (MQ=255)
gcgGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCa < 1:3143996/68‑1 (MQ=255)
gcgGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCa < 1:2319841/68‑1 (MQ=255)
gcgGACAGCGAAAAATATATGCAGGGTCAGGTGACACCAGATGTCAGCCAGATGGCAGAAGATCAGCa < 1:2197716/68‑1 (MQ=255)
cgGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCa < 1:2148002/67‑1 (MQ=255)
cAGCGAAAAATATATGCAAGGCCAGGTGACAGCAGATGTCAGCCAGATGACTGAAGATCAGCACCTGCt < 1:2523523/69‑1 (MQ=255)
cAGCGAAAAATATATGCAAGGCCAGGTGACAGCAGATGTCAGCCAGATGACTGAAGATCAGCACCTGCt < 1:1299593/69‑1 (MQ=255)
cAGCGAAAAATATATGCAAGGCCAGGTGACAGCAGATGTCAGCCAGATGACTGAAGATCAGCACCTGCt < 1:798537/69‑1 (MQ=255)
cAGCGAAAAATATATGCAAGGCCAGGTGACAGCAGATGTCAGCCAGATGACTGAAGATCAGCACCTGCt < 1:872869/69‑1 (MQ=255)
cAGCGAAAAATATATGCAAGGCCAGGTGACAGCAGATGTCAGCCAGATGACTGAAGATCAGCACCTGCt < 1:1146191/69‑1 (MQ=255)
cAGCGAAAAATATATGCAAGGCCAGGTGACAGCAGATGTCAGCCAGATGACTGAAGATCAGCACCTGCt < 1:1101832/69‑1 (MQ=255)
atatatGCAAGGCCAGGTGACAGCAGATGTCAGCCAGATGACTGAAGATCAGCACCTGCTcgccgc > 1:3123724/1‑66 (MQ=255)
atatatGCAAGGCCAGGTGACAGCAGATGTCAGCCAGATGACTGAAGATCAGCACCTGCTCGCCGccc > 1:401785/1‑68 (MQ=255)
atatatGCAAGGCCAGGTGACAGCAGATGTCAGCCAGATGACTGAAGATCAGCACCTGCTCGCCGCCCa > 1:2788958/1‑69 (MQ=255)
atatatGCAAGGCCAGGTGACAGCAGATGTCAGCCAGATGACTGAAGATCAGCACCTGCTCGCCGCCCa > 1:2997910/1‑69 (MQ=255)
atatatGCAAGGCCAGGTGACAGCAGATGTCAGCCAGATGACTGAAGATCAGCACCTGCTCGCCGCCCa > 1:2442312/1‑69 (MQ=255)
atatatGCAAGGCCAGGTGACAGCAGATGTCAGCCAGATGACTGAAGATCAGCACCTGCTCGCCGCCCa > 1:45782/1‑69 (MQ=255)
atatatGCAAGGCCAGGTGACAGCAGATGTCAGCCAGATGACTGAAGATCAGCACCTGCTCGCCGCCCa > 1:77759/1‑69 (MQ=255)
atatatGCAAGGCCAGGTGACAGCAGATGTCAGCCAGATGACTGAAGATCAGCACCTGCTCGCCGCCCa > 1:2590218/1‑69 (MQ=255)
atatatGCAAGGCCAGGTGACAGCAGATGTCAGCCAGATGACTGAAGATCAGCACCTGCTCGCCGCCCa > 1:7788/1‑69 (MQ=255)
atatatGCAAGGCCAGGTGACAGCAGATGTCAGCCAGATGACTGAAGATCAGCACCTGCTCGCCGCCCa > 1:969133/1‑69 (MQ=255)
atatatGCAAGGCCAGGTGACAGCAGATGTCAGCCAGATGACTGAAGATCAGCACCTGCTCGCCGCCCa > 1:1032034/1‑69 (MQ=255)
atatatGCAAGGCCAGGTGACAGCAGATGTCAGCCAGATGACTGAAGATCAGCACCTGCTCGCCGCCCa > 1:12029/1‑69 (MQ=255)
atatatGCAAGGCCAGGTGACAGCAGATGTCAGCCAGATGACTGAAGATCAGCACCTGCTCGCCGCCCa > 1:1682378/1‑69 (MQ=255)
atatatGCAAGGCCAGGTGACAGCAGATGTCAGCCAGATGACTGAAGATCAGCACCTGCTCGCCGCCCa > 1:1973466/1‑69 (MQ=255)
atatatGCAAGGCCAGGTGACAGCAGATGTCAGCCAGATGACTGAAGATCAGCACCTGCTCGCCGCCCa > 1:1468270/1‑69 (MQ=255)
atatatGCAAGGCCAGGTGACAGCAGATGTCAGCCAGATGACTGAAGATCAGCACCTGCTCGCCGCCCa > 1:1179301/1‑69 (MQ=255)
atatatGCAAGGCCAGGTGACAGCAGATGTCAGCCAGATGACTGAAGATCAGCACCTGCTCGCCGCCCa > 1:2044883/1‑69 (MQ=255)
atatatGCAAGGCCAGGTGACAGCAGATGTCAGCCAGATGACTGAAGATCAGCACCTGCTCGCCGCCCa > 1:2129011/1‑69 (MQ=255)
tGACAGCAGATGTCAGCCATATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGt > 1:2459261/1‑68 (MQ=255)
tGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCAtt > 1:1335464/1‑54 (MQ=255)
tGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGt > 1:852968/1‑68 (MQ=255)
tGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGt > 1:54084/1‑68 (MQ=255)
gACAGCAGATGTCAGCCAGATGGCATAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTaa > 1:11476/1‑69 (MQ=255)
gACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGt > 1:224024/1‑67 (MQ=255)
gACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTaa > 1:995108/1‑69 (MQ=255)
gACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTaa > 1:1382514/1‑69 (MQ=255)
gACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTaa > 1:2330921/1‑69 (MQ=255)
gACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTaa > 1:531699/1‑69 (MQ=255)
gACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTaa > 1:1495388/1‑69 (MQ=255)
gACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTaa > 1:496911/1‑69 (MQ=255)
gACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTaa > 1:1737244/1‑69 (MQ=255)
gACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTaa > 1:2042025/1‑69 (MQ=255)
gACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTaa < 1:2820166/69‑1 (MQ=255)
gACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTaa > 1:2835368/1‑69 (MQ=255)
gACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTaa > 1:2862709/1‑69 (MQ=255)
gACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTaa < 1:1927984/69‑1 (MQ=255)
gACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTaa > 1:3214738/1‑69 (MQ=255)
gcagATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAAtg > 1:326742/1‑69 (MQ=255)
gcagATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAAtg > 1:457849/1‑69 (MQ=255)
gcagATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAAtg > 1:2796455/1‑69 (MQ=255)
gcagATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAAtg > 1:2303892/1‑69 (MQ=255)
gcagATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCAATTGCGACGCCAAAGGTaaaa > 1:1646767/1‑67 (MQ=255)
gATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTgg < 1:376730/69‑1 (MQ=255)
gTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGccc < 1:500998/40‑1 (MQ=255)
gTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGccc < 1:2863339/40‑1 (MQ=255)
cAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTa > 1:190030/1‑69 (MQ=255)
cAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTa > 1:2002756/1‑69 (MQ=255)
|
TTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTA > minE/1940675‑1940799
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
