Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,963,872 |
C→G |
49.1% |
V50V (GTG→GTC) |
yggD ← |
predicted DNA‑binding transcriptional regulator |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,963,872 | 0 | C | G | 49.1%
| ‑4.6
/ 131.5
| 104 | V50V (GTG→GTC) | yggD | predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base C (28/25); new base G (30/21); total (58/46) |
| Fisher's exact test for biased strand distribution p-value = 5.60e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.50e-01 |
Only 100 of 126 total aligned reads displayed.
CAGTGCAACATCAATATCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGCGTATCCCCTGGAGCAAAATGG > minE/1963809‑1963939
|
cAGTGCAACATCAATATCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCCACGAt > 1:1018968/1‑69 (MQ=255)
cAGTGCAACATCAATATCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCCACGAt > 1:3181262/1‑69 (MQ=255)
cAGTGCAACATCAATATCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCCACGAt > 1:1165025/1‑69 (MQ=255)
aaCATCAATATCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCGACGATCTCTTc < 1:2671052/69‑1 (MQ=255)
aaCATCAATATCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCGACGATCTCTTc < 1:1054551/69‑1 (MQ=255)
aaCATCAATATCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCGACGATCTCTTc < 1:486047/69‑1 (MQ=255)
aaCATCAATATCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCGACGATCTCTTc < 1:609038/69‑1 (MQ=255)
aaCATCAATATCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCGACGATCTCTTc < 1:738752/69‑1 (MQ=255)
aaCATCAATATCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCGACGATCTCTTc < 1:2577241/69‑1 (MQ=255)
aaCATCAATATCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCGACGATCTCTTc < 1:1228146/69‑1 (MQ=255)
aaCATCAATATCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCGACGATCTCTTc < 1:740531/69‑1 (MQ=255)
aCATCAATATCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCGACGATCTCTTc < 1:2403085/68‑1 (MQ=255)
aCATCAATATCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCGACGATCTCTTc < 1:1505912/68‑1 (MQ=255)
aCATCAATATCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCCACGATCTCTTCa > 1:2172412/1‑69 (MQ=255)
tCAATATCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCCACGATctct > 1:250090/1‑63 (MQ=255)
tCAATATCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCCACGATCTCTTCATc > 1:1432509/1‑68 (MQ=255)
tCAATATCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCCACGATCTCTTCATc > 1:315318/1‑68 (MQ=255)
tCAATATCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCCACGATCTCTTCATc > 1:852729/1‑68 (MQ=255)
tCAATATCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCCACGATCTCTTCATc > 1:1869197/1‑68 (MQ=255)
atCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCGACGATCTCTTCATCGtt > 1:380746/1‑66 (MQ=255)
atCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCGACGATCTCTTCATCGTTAt > 1:2294240/1‑68 (MQ=255)
atCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCGACGATCTCTTCATCGTTATc > 1:1984226/1‑69 (MQ=255)
atCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCGACGATCTCTTCATCGTTATc > 1:1925897/1‑69 (MQ=255)
atCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCGACGATCTCTTCATCGTTATc > 1:1825878/1‑69 (MQ=255)
atCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCGACGATCTCTTCATCGTTATc > 1:2003473/1‑69 (MQ=255)
atCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCGACGATCTCTTCATCGTTATc > 1:1925204/1‑69 (MQ=255)
atCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCGACGATCTCTTCATCGTTATc > 1:3391765/1‑69 (MQ=255)
atCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCGACGATCTCTTCATCGTTATc > 1:1594091/1‑69 (MQ=255)
atCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCGACGATCTCTTCATCGTTATc > 1:1529214/1‑69 (MQ=255)
atCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCGACGATCTCTTCATCGTTATc > 1:2110837/1‑69 (MQ=255)
atCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCGACGATCTCTTCATCGTTATc > 1:1463708/1‑69 (MQ=255)
atCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCGACGATCTCTTCATCGTTATc > 1:2544214/1‑69 (MQ=255)
atCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCGACGATCTCTTCATCGTTATc > 1:894171/1‑69 (MQ=255)
atCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCGACGATCTCTACATCGTTATc > 1:1099605/1‑69 (MQ=255)
aacGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCGACGATCTCTTCATCGTTATc > 1:628356/3‑69 (MQ=255)
tCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATc > 1:2059762/1‑68 (MQ=255)
tCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATc > 1:1009262/1‑68 (MQ=255)
tCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATc > 1:765565/1‑68 (MQ=255)
tCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATc > 1:1167972/1‑68 (MQ=255)
tCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATc > 1:1204747/1‑68 (MQ=255)
tCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATc > 1:149616/1‑68 (MQ=255)
cAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCGACGATCTCTTCATCGTTATCGACaa < 1:1336983/69‑1 (MQ=255)
cAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCGACGATCTCTTCATCGTTATCGACaa < 1:2460153/69‑1 (MQ=255)
cAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCGACGATCTCTTCATCGTTATCGACaa < 1:1027184/69‑1 (MQ=255)
cAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCGACGATCTCTTCATCGTTATCGACaa < 1:2318549/69‑1 (MQ=255)
cAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCGACGATCTCTTCATCGTTATCGACaa < 1:2909345/69‑1 (MQ=255)
cAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCGACGATCTCTTCATCGTTATCGACaa < 1:429789/69‑1 (MQ=255)
cAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCGACGATCTCTTCATCGTTATCGACaa < 1:420700/69‑1 (MQ=255)
aGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCGACGATCTCTTCATCGTTATCGACaa < 1:3357047/67‑1 (MQ=255)
ggCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCg < 1:2305015/69‑1 (MQ=255)
ggCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCg < 1:3055037/69‑1 (MQ=255)
gCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCg < 1:2757521/68‑1 (MQ=255)
cGCTTTGGGCGAGTAACGGCTTCACTGCATATTcc > 1:3125079/1‑35 (MQ=255)
cGCTTTGGGCGAGTAACGGCTTAACTGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCGAc > 1:924933/1‑68 (MQ=255)
tttGGGCGAGTAACGGCTTCACTGCATATTCGACGATCTCTTCATCGTTATCGACAAACAGCGACgg > 1:285054/1‑67 (MQ=255)
tttGGGCGAGTAACGGCTTCACTGCATATTCGACGATCTCTTCATCGTTATCGACAAACAGCGACGGc > 1:2414433/1‑68 (MQ=255)
tttGGGCGAGTAACGGCTTCACTGCATATTCGACGATCTCTTCATCGTTATCGACAAACAGCGACGGc > 1:16365/1‑68 (MQ=255)
tttGGGCGAGTAACGGCTTCACTGCATATTCGACGATCTCTTCATCGTTATCGACAAACAGCGACGGc > 1:254648/1‑68 (MQ=255)
tttGGGCGAGTAACGGCTTCACTGCATATTCGACGATCTCTTCATCGTTATCGACAAACAGCGACGGc > 1:2581882/1‑68 (MQ=255)
ggCGAGTAACGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGa < 1:657277/67‑1 (MQ=255)
aGTAACGGCTTCCCTGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTgg < 1:282261/69‑1 (MQ=255)
aGTAACGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTgg < 1:1642687/69‑1 (MQ=255)
aGTAACGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTgg < 1:1480773/69‑1 (MQ=255)
aGTAACGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTgg < 1:176875/69‑1 (MQ=255)
aGTAACGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTgg < 1:178894/69‑1 (MQ=255)
gTAACGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTgg < 1:2756311/68‑1 (MQ=255)
aaCGGCTTCTCTGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGCGt < 1:488084/69‑1 (MQ=255)
aaCGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGCGt < 1:135289/69‑1 (MQ=255)
aaCGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGCGt < 1:2966662/69‑1 (MQ=255)
aaCGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGCGt < 1:1137552/69‑1 (MQ=255)
aaCGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGCGt < 1:2110091/69‑1 (MQ=255)
aaCGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGCGt < 1:2582124/69‑1 (MQ=255)
aaCGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGCGt < 1:424020/69‑1 (MQ=255)
aCGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGCGTa > 1:3083822/1‑69 (MQ=255)
aCGGCTTCACCGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGCGt < 1:510283/68‑1 (MQ=255)
tCACTGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGCGt < 1:1164488/62‑1 (MQ=255)
cATATTCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGCGTATCCCCTGGAGc > 1:2357770/1‑68 (MQ=255)
atTCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGCGt < 1:1534333/53‑1 (MQ=255)
ttCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGCGTATCCCCTGGAGCAAAAt > 1:879314/1‑69 (MQ=255)
ttCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGCGTATCCCCTGGAGCAAAAt > 1:537536/1‑69 (MQ=255)
ttCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGCGTATCCCCTGGAGCAAAAt > 1:1578701/1‑69 (MQ=255)
ttCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGAGTATCCCCTGGAGCAAAAt > 1:2939898/1‑69 (MQ=255)
ccACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGa > 1:1007188/1‑41 (MQ=255)
ccACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGa > 1:2470025/1‑41 (MQ=255)
ccACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGa > 1:1514930/1‑41 (MQ=255)
ccACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGa > 1:1398769/1‑41 (MQ=255)
ccACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGa > 1:1138283/1‑41 (MQ=255)
ccACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGa > 1:153898/1‑41 (MQ=255)
ccACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGa > 1:3262163/1‑41 (MQ=255)
ccACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGa > 1:1695429/1‑41 (MQ=255)
ccACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGa > 1:1884985/1‑41 (MQ=255)
ccACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGa > 1:2063359/1‑41 (MQ=255)
ccACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGa > 1:2718731/1‑41 (MQ=255)
ccACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGCGTATCCCCTGGAGCAAAATgg < 1:407122/69‑1 (MQ=255)
ccACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGCGTATCCCCTGGAGCAAAATgg < 1:579164/69‑1 (MQ=255)
ccACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGCGTATCCCCTGGAGCAAAATgg < 1:1668426/69‑1 (MQ=255)
ccACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGCGTATCCCCTGGAGCAAAATgg < 1:1774353/69‑1 (MQ=255)
ccACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGCGTATCCCCTGGAGCAAAATgg < 1:2832704/69‑1 (MQ=255)
ccACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGCGTATCCCCTGGAGCAAAATgg < 1:2744410/69‑1 (MQ=255)
ccACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGCGTATCCCCTGGAGCAAAATgg < 1:2269564/69‑1 (MQ=255)
|
CAGTGCAACATCAATATCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGCGTATCCCCTGGAGCAAAATGG > minE/1963809‑1963939
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
