Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,990,804 |
C→T |
60.0% |
D266D (GAC→GAT) |
mutY → |
adenine DNA glycosylase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,990,804 | 0 | C | T | 60.0%
| 10.8
/ 21.6
| 35 | D266D (GAC→GAT) | mutY | adenine DNA glycosylase |
| Reads supporting (aligned to +/- strand): ref base C (6/8); new base T (12/9); total (18/17) |
| Fisher's exact test for biased strand distribution p-value = 5.00e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.18e-01 |
TTGCTGGCGCAGCGTCCGCCGAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCG > minE/1990739‑1990850
|
ttGCTGGCGCAGCGTCCGCCGAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCcgacga > 1:422896/1‑68 (MQ=255)
gCTGGCGCAGCGTCCGCCGAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACgaa > 1:249854/1‑67 (MQ=255)
gCTGGCGCAGCGTCCGCCGAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACgaa > 1:1826213/1‑67 (MQ=255)
gCTGGCGCAGCGTCCGCCGAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACgaa > 1:1709592/1‑67 (MQ=255)
gCTGGCGCAGCGTCCGCCGAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACgaa > 1:1292614/1‑67 (MQ=255)
gATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGgcgc < 1:2895396/69‑1 (MQ=255)
gATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGgcgc < 1:1190973/69‑1 (MQ=255)
aTTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGATGAAGAAAGTTTGCGGc < 1:1238208/56‑1 (MQ=255)
aTTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGATGAAGAAAGTTTGCGGc < 1:1140228/56‑1 (MQ=255)
aTTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGATGAAGAAAGTTTGCGGc < 1:86625/56‑1 (MQ=255)
aTTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGATGAAGAAAGTTTGCGGc < 1:591422/56‑1 (MQ=255)
aTTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGATGAAGAAAGTTTGCGGc < 1:2709487/56‑1 (MQ=255)
aTTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGATGAAGAAAGTTTGCGGc < 1:2705676/56‑1 (MQ=255)
aTTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGATGAAGAAAGTTTGCGGc < 1:2370420/56‑1 (MQ=255)
aTTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGATGAAGAAAGTTTGCGGc < 1:2696811/56‑1 (MQ=255)
tgtgtgGCGGTTTATACTGTTTCCCGCAGTTTGCCGATGAAGAAAGTTTGCGGc < 1:2497224/54‑1 (MQ=255)
tgtgGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGc > 1:1416088/1‑54 (MQ=255)
gggCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCa < 1:1001024/69‑1 (MQ=255)
gggCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCa < 1:2345795/69‑1 (MQ=255)
gCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAg < 1:2421730/68‑1 (MQ=255)
gCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAg < 1:2361877/68‑1 (MQ=255)
gCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAg < 1:2978657/68‑1 (MQ=255)
cGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAg < 1:1284837/67‑1 (MQ=255)
tataCTGTTTCCCGCAGTTTGCCGATGAAGAAAGTTTGCGGCAGTGGCTGGCGCAAc > 1:572377/1‑57 (MQ=255)
tataCTGTTTCCCGCAGTTTGCCGATGAAGAAAGTTTGCGGCAGTGGCTGGCGCAAc > 1:1038267/1‑57 (MQ=255)
tataCTGTTTCCCGCAGTTTGCCGATGAAGAAAGTTTGCGGCAGTGGCTGGCGCAAc > 1:2579327/1‑57 (MQ=255)
tataCTGTTTCCCGCAGTTTGCCGATGAAGAAAGTTTGCGGCAGTGGCTGGCGCAAc > 1:1060487/1‑57 (MQ=255)
tataCTGTTTCCCGCAGTTTGCCGATGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGACAGATtgctg > 1:3065777/1‑69 (MQ=255)
tataCTGTTTCCCGCAGTTTGCCGATGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGACAGATtgctg > 1:3136163/1‑69 (MQ=255)
tataCTGTTTCCCGCAGTTTGCCGATGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGACAGATtgctg > 1:3263653/1‑69 (MQ=255)
tataCTGTTTCCCGCAGTTTGCCGATGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGACAGATtgctg > 1:3379563/1‑69 (MQ=255)
tataCTGTTTCCCGCAGTTTGCCGATGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGACAGATtgctg > 1:2898857/1‑69 (MQ=255)
tataCTGTTTCCCGCAGTTTGCCGATGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGACAGATtgctg > 1:559821/1‑69 (MQ=255)
tataCTGTTTCCCGCAGTTTGCCGATGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGACAGATtgctg > 1:215937/1‑69 (MQ=255)
tataCTGTTTCCCGCAGTTTGCCGATGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGACAGATtgctg > 1:957458/1‑69 (MQ=255)
cTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCg > 1:733463/1‑68 (MQ=255)
|
TTGCTGGCGCAGCGTCCGCCGAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCG > minE/1990739‑1990850
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
