Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F1 I0 R1 4577 57.4 3436498 77.7% 2670158 63.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,993,811 T→G 30.0% V368V (GTT→GTG)  nupG → nucleoside transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,993,8110TG30.0% 100.6 / 68.5 100V368V (GTT→GTG) nupGnucleoside transporter
Reads supporting (aligned to +/- strand):  ref base T (37/33);  new base G (16/14);  total (53/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01

Only 100 of 114 total aligned reads displayed.

GATGTTCCTGATGATGACTAACGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATGTACACCCAAAACGGCATTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTA  >  minE/1993748‑1993876
                                                               |                                                                 
gctGTTCCTGATGATGACTAACGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTga                                                              <  1:3074570/67‑1 (MQ=255)
gATGTTCCTGATGATGACTAACGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTga                                                              <  1:2202197/69‑1 (MQ=255)
gATGTTCCTGATGATGACTAACGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTga                                                              <  1:2015996/69‑1 (MQ=255)
gATGTTCCTGATGATGACTAACGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTga                                                              <  1:462463/69‑1 (MQ=255)
 aTGTTCCTGATGATGACTAACGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTgag                                                             <  1:2454773/69‑1 (MQ=255)
 aTGTTCCTGATGATGACTAACGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTgag                                                             <  1:2976448/69‑1 (MQ=255)
 aTGTTCCTGATGATGACTAACGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTgag                                                             <  1:2608194/69‑1 (MQ=255)
    ttCCTGATGATGACTAACGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATg                                                          >  1:3217286/1‑69 (MQ=255)
    ttCCTGATGATGACTAACGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATg                                                          >  1:1821603/1‑69 (MQ=255)
    ttCCTGATGATGACTAACGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATg                                                          >  1:2171410/1‑69 (MQ=255)
    ttCCTGATGATGACTAACGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATg                                                          >  1:1497703/1‑69 (MQ=255)
    ttCCTGATGATGACTAACGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATg                                                          >  1:2069644/1‑69 (MQ=255)
      ccTGATGATGACTAACGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATGt                                                         >  1:206193/1‑68 (MQ=255)
      ccTGATGATGACTAACGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATGTa                                                        >  1:497437/1‑69 (MQ=255)
        tgatgatgaCTAACGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATGTac                                                       <  1:655532/68‑1 (MQ=255)
         gatgatgaCTAACGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATGTaca                                                      <  1:3367387/68‑1 (MQ=255)
         gatgatgaCTAACGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATGTaca                                                      <  1:2282025/68‑1 (MQ=255)
         gatgatgaCTAACGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATGTaca                                                      <  1:861840/68‑1 (MQ=255)
         gatgatgaCTAACGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATGTaca                                                      <  1:1798249/68‑1 (MQ=255)
         gatgatgaCTAACGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATGTaca                                                      <  1:40071/68‑1 (MQ=255)
         gatgatgaCTAACGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATGTaca                                                      <  1:2868107/68‑1 (MQ=255)
         gatgatgaCTAACGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATGTaca                                                      <  1:1389770/68‑1 (MQ=255)
         gatgatgaCTAACGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATGTac                                                       <  1:3094465/67‑1 (MQ=255)
          atgatgaCTAACGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATGTaca                                                      <  1:2761242/67‑1 (MQ=255)
            gatgaCTAACGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATGTACAccc                                                   >  1:2460845/1‑68 (MQ=255)
            gatgaCTAACGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATGTACAccc                                                   >  1:3262480/1‑68 (MQ=255)
            gatgaCTAACGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATGTACAccc                                                   >  1:3341521/1‑68 (MQ=255)
            gatgaCTAACGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATGTACAccc                                                   >  1:1177821/1‑68 (MQ=255)
             atgaCTAACGGCTTCGGCTGTATCCTCGGCGGCATCGTTAGCGGTAAAGTTGTTGAGATg                                                          >  1:3427381/1‑60 (MQ=255)
             atgaCTAACGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATg                                                          >  1:1065291/1‑60 (MQ=255)
             atgaCTAACGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATg                                                          >  1:3219944/1‑60 (MQ=255)
             atgaCTAACGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATg                                                          >  1:656691/1‑60 (MQ=255)
             atgaCTAACGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATg                                                          >  1:1855208/1‑60 (MQ=255)
             atgaCTAACGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATg                                                          >  1:1732644/1‑60 (MQ=255)
             atgaCTAACGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATg                                                          >  1:1652622/1‑60 (MQ=255)
             atgaCTAACGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATg                                                          >  1:544318/1‑60 (MQ=255)
             atgaCTAACGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATg                                                          >  1:1409608/1‑60 (MQ=255)
             atgaCTAACGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATg                                                          >  1:2613017/1‑60 (MQ=255)
                   aaCGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCTGTAAAGTTGTTGAGATGTACACCCAAAACGGc                                           <  1:3379417/69‑1 (MQ=255)
                   aaCGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATGTACACCCAAAACGGc                                           <  1:1941564/69‑1 (MQ=255)
                   aaCGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATGTACACCCAAAACGGc                                           <  1:2694457/69‑1 (MQ=255)
                   aaCGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATGTACACCCAAAACGGc                                           <  1:2794859/69‑1 (MQ=255)
                   aaCGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATGTACACCCAAAACGGc                                           <  1:3234485/69‑1 (MQ=255)
                   aaCGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATGTACACCCAAAACGGc                                           <  1:1155990/69‑1 (MQ=255)
                   aaCGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATGTACACCCAAAACGGc                                           <  1:820661/69‑1 (MQ=255)
                   aaCGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATGTACACCCAAAACGGc                                           <  1:552382/69‑1 (MQ=255)
                   aaCGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATGTACACCCAAAACGGc                                           <  1:1542042/69‑1 (MQ=255)
                    aCGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATGTACACCCAAAACGGc                                           >  1:231420/1‑68 (MQ=255)
                    aCGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATGTACACCCAAAACGGc                                           >  1:2698255/1‑68 (MQ=255)
                    aCGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATGTACACCCAAAACGGc                                           >  1:317631/1‑68 (MQ=255)
                    aCGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATGTACACCCAAAACGGc                                           >  1:1049362/1‑68 (MQ=255)
                      ggCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATGTACACCCAAAACGGCAtt                                        <  1:2800700/69‑1 (MQ=255)
                           cGGCTGTATCCTCGGCTGCATCGTGAGCGGTAAAGTTGTTGAGATGTACACCCAAAACGGc                                           <  1:1750987/61‑1 (MQ=255)
                                  aTCCTCGGCGGCATCGTGAGCGGTAATGTGGTTgag                                                             >  1:1336193/1‑36 (MQ=37)
                                  aTCCTCGGCGGCATCGTGAGCGGTAAAGTGGTTGAGATGTACACCTAAAACGGCACTACCGACTGGCAg                            >  1:374088/1‑69 (MQ=255)
                                  aTCCTCGGCGGCATCGTGAGCGGTAAAGTGGTTGAGATGTACACCCAAAACGGCACTAcc                                     >  1:2666023/1‑60 (MQ=255)
                                  aTCCTCGGCGGCATCGTGAGCGGTAAAGTGGTTGAGATGTACACCCAAAACGGCACTACCGACTGGCAg                            >  1:3386596/1‑69 (MQ=255)
                                  aTCCTCGGCGGCATCGTGAGCGGTAAAGTGGTTGAGATGTACACCCAAAACGGCACTACCGACTGGCAg                            >  1:2922787/1‑69 (MQ=255)
                                  aTCCTCGGCGGCATCGTGAGCGGTAAAGTGGTTGAGATGTACACCCAAAACGGCACTACCGACTGGCAg                            >  1:687349/1‑69 (MQ=255)
                                  aTCCTCGGCGGCATCGTGAGCGGTAAAGTGGTTGAGATGTACACCCAAAACGGCACTACCGACTGGCAg                            >  1:964028/1‑69 (MQ=255)
                                  aTCCTCGGCGGCATCGTGAGCGGTAAAGTGGTTGAGATGTACACCCAAAACGGCACTACCGACTGGCAg                            >  1:2601300/1‑69 (MQ=255)
                                  aTCCTCGGCGGCATCGTGAGCGGTAAAGTGGTTGAGATGTACACCCAAAACGGCACTACCGACTGGCAg                            >  1:1772706/1‑69 (MQ=255)
                                  aTCCTCGGCGGCATCGTGAGCGGTAAAGTGGTTGAGATGTACACCCAAAACGGCACTACCGACTGGCAg                            >  1:1426522/1‑69 (MQ=255)
                                  aTCCTCGGCGGCATCGTGAGCGGTAAAGTGGTTGAGATGTACACCCAAAACGGCACTACCGACTGGCAg                            >  1:2006810/1‑69 (MQ=255)
                                  aTCCTCGGCGGCATCGTGAGCGGTAAAGTGGTTGAGATGTACACCCAAAACGGCACTACCGACTGGCAg                            >  1:2189478/1‑69 (MQ=255)
                                  aTCCTCGGCGGCATCGTGAGCGGTAAAGTGGTTGAGATGTACACCCAAAACGGCACTACCGACTGGCAg                            >  1:1173929/1‑69 (MQ=255)
                                  aTCCTCGGCGGCATCGTGAGCGGTAAAGTGGTTGAGATGTACACCCAAAACGGCACTACCGACTGGCAg                            >  1:2773734/1‑69 (MQ=255)
                                   tCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATGTACACCCAAAACGGCATTACCGACTGGCAGa                           >  1:2353839/1‑69 (MQ=255)
                                       cggcggCATCGTGAGCGGTAAAGTTGTTGAGATGTACACCCAAAACGGCATTACCGACTGGCTGACCg                        <  1:2339676/68‑1 (MQ=255)
                                                 gTGAGCGGTAAAGTTGTTGAGATGTACACCCAAAACGGCATTACCGACTGGCAg                            >  1:642237/1‑54 (MQ=255)
                                                 gTGAGCGGTAAAGTTGTTGAGATGTACACCCAAAACGGCATTACCGACTGGCAg                            >  1:2189329/1‑54 (MQ=255)
                                                 gTGAGCGGTAAAGTTGTTGAGATGTACACCCAAAACGGCATTACCGACTGGCAg                            >  1:2039189/1‑54 (MQ=255)
                                                 gTGAGCGGTAAAGTTGTTGAGATGTACACCCAAAACGGCATTACCGACTGGCAg                            >  1:96325/1‑54 (MQ=255)
                                                 gTGAGCGGTAAAGTTGTTGAGATGTACACCCAAAACGGCATTACCGACTGGCAg                            >  1:3297661/1‑54 (MQ=255)
                                                 gTGAGCGGTAAAGTTGTTGAGATGTACACCCAAAACGGCATTACCGACTGGCAg                            >  1:1970069/1‑54 (MQ=255)
                                                 gTGAGCGGTAAAGTTGTTGAGATGTACACCCAAAACGGCATTACCGACTGGCAg                            >  1:1663847/1‑54 (MQ=255)
                                                      cGGTAAAGTGGTTGAGATGTACACCCAAAACGGCACTACCGACTGGCAGACCGTATGGCTGATTTTct         <  1:3008174/68‑2 (MQ=255)
                                                      cGGTAAAGTGGTTGAGATGTACACCCAAAACGGCACTACCGACTGGCAGACCGTATGGCTGATTTTCg         <  1:117217/68‑1 (MQ=255)
                                                      cGGTAAAGTGGTTGAGATGTACACCCAAAACGGCACTACCGACTGGCAGACCGTATGGCTGATTTTCg         <  1:841373/68‑1 (MQ=255)
                                                      cGGTAAAGTGGTTGAGATGTACACCCAAAACGGCACTACCGACTGGCAGACCGTATGGCTGATTTTCg         <  1:1421420/68‑1 (MQ=255)
                                                      cGGTAAAGTGGTTGAGATGTACACCCAAAACGGCACTACCGACTGGCAGACCGTATGGCTGATTTTCg         <  1:948939/68‑1 (MQ=255)
                                                      cGGTAAAGTGGTTGAGATGTACACCCAAAACGGCACTACCGACTGGCAGACCGTATGGCTGATTTTCg         <  1:1001325/68‑1 (MQ=255)
                                                      cGGTAAAGTGGTTGAGATGTACACCCAAAACGGCACTACCGACTGGCAGACCGTATGGCTGATTTTCg         <  1:2810172/68‑1 (MQ=255)
                                                      cGGTAAAGTGGTTGAGATGTACACCCAAAACGGCACTACCGACTGGCAGACCGTATGGCTGATTTTCg         <  1:2843570/68‑1 (MQ=255)
                                                      cGGTAAAGTGGTTGAGATGTACACCCAAAACGGCACTACCGACTGGCAGACCGTATGGCTGATTTTCg         <  1:2210093/68‑1 (MQ=255)
                                                      cGGTAAAGTGGTTGAGATGTACACCCAAAACGGCACTACCGACTGGCAGACCGTATGGCTGATTTTCg         <  1:3126880/68‑1 (MQ=255)
                                                      cGGTAAAGTGGTTGAGATGTACACCCAAAACGGCACTACCGACTGGCAGACCGTATGGCTGATTTTCg         <  1:2578786/68‑1 (MQ=255)
                                                      cGGTAAAGTGGTTGAGATGTACACCCAAAACGGCACTACCGACTGGCAGACCGTATGGCTGATTTTCg         <  1:1849119/68‑1 (MQ=255)
                                                       ggTAAAGTGGTTGAGATGTACACCCAAAACGGCACTACCGACTGGCAGACCGTATGGCTGATTTTCg         <  1:1930717/67‑1 (MQ=255)
                                                             gttgttGAGATGTACACCCAAAACGGCATTACCGACTGGCAGACCGTATGGTTGATTTTCTCTGGTTa  <  1:1123483/68‑1 (MQ=255)
                                                             gttgttGAGATGTACACCCAAAACGGCATTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTa  <  1:1203594/68‑1 (MQ=255)
                                                             gttgttGAGATGTACACCCAAAACGGCATTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTa  <  1:2591891/68‑1 (MQ=255)
                                                             gttgttGAGATGTACACCCAAAACGGCATTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTa  <  1:2947070/68‑1 (MQ=255)
                                                             gttgttGAGATGTACACCCAAAACGGCATTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTa  <  1:809679/68‑1 (MQ=255)
                                                             gttgttGAGATGTACACCCAAAACGGCATTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTa  <  1:2270046/68‑1 (MQ=255)
                                                             gttgttGAGATGTACACCCAAAACGGCATTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTa  <  1:1301331/68‑1 (MQ=255)
                                                             gttgttGAGATGTACACCCAAAACGGCATTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTa  <  1:1943535/68‑1 (MQ=255)
                                                             gttgttGAGATGTACACCCAAAACGGCATTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTa  <  1:1723797/68‑1 (MQ=255)
                                                             gttgttGAGATGTACACCCAAAACGGCATTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTa  <  1:1862185/68‑1 (MQ=255)
                                                             gttgttGAGATGTACACCCAAAACGGCATTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTa  <  1:1815639/68‑1 (MQ=255)
                                                               |                                                                 
GATGTTCCTGATGATGACTAACGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATGTACACCCAAAACGGCATTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTA  >  minE/1993748‑1993876

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A