Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F1 I0 R1 4577 57.4 3436498 77.7% 2670158 63.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,000,404 G→A 43.6% I293I (ATC→ATT)  gss ← fused glutathionylspermidine amidase and glutathionylspermidine synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,000,4040GA43.6% 37.3 / 92.0 119I293I (ATC→ATT) gssfused glutathionylspermidine amidase and glutathionylspermidine synthetase
Reads supporting (aligned to +/- strand):  ref base G (31/36);  new base A (23/29);  total (54/65)
Fisher's exact test for biased strand distribution p-value = 8.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 122 total aligned reads displayed.

CGACCAGTGATCATATGGTGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGGATGTCGAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTGTCGGTTGCGTGAAGATACATCA  >  minE/2000336‑2000468
                                                                    |                                                                
cGACCAGTGATCATATGGTGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCggg                                                                  >  1:964590/1‑69 (MQ=255)
cGACCAGTGATCATATGGTGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCggg                                                                  >  1:775082/1‑69 (MQ=255)
         aTCATATGGTGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGGATGTCGaa                                                          >  1:1211389/1‑68 (MQ=255)
         aTCATATGGTGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGGATGTCGAAc                                                         >  1:2304579/1‑69 (MQ=255)
         aTCATATGGTGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGGATGTCGAAc                                                         >  1:3114384/1‑69 (MQ=255)
         aTCATATGGTGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGGATGTCGAAc                                                         >  1:3155430/1‑69 (MQ=255)
         aTCATATGGTGACAGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGGATGTCGAAc                                                         >  1:1318246/1‑69 (MQ=255)
         aTCATATGATGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGAATGTCAAAc                                                         >  1:1012029/1‑69 (MQ=255)
         aTCATATGATGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGAATGTCAAAc                                                         >  1:2062611/1‑69 (MQ=255)
         aTCATATGATGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGAATGTCAAAc                                                         >  1:216071/1‑69 (MQ=255)
         aTCATATGATGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGAATGTCAAAc                                                         >  1:2245340/1‑69 (MQ=255)
         aTCATATGATGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGAATGTCAAAc                                                         >  1:2957400/1‑69 (MQ=255)
         aTCATATGATGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGAATGTCAAAc                                                         >  1:2983156/1‑69 (MQ=255)
         aTCATATGATGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGAATGTCAAAc                                                         >  1:1603455/1‑69 (MQ=255)
         aTCATATGATGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGAATGTCAAAc                                                         >  1:1577229/1‑69 (MQ=255)
         aTCATATGATGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGAATGTCAAAc                                                         >  1:3265999/1‑69 (MQ=255)
         aTCATATGATGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGAATGTCAAAc                                                         >  1:620158/1‑69 (MQ=255)
         aTCATATGATGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGAATGTCAAAc                                                         >  1:1147329/1‑69 (MQ=255)
         aTCATATGATGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGAATGTCAAAc                                                         >  1:890443/1‑69 (MQ=255)
         aTCATATGATGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGAATGTCAAAc                                                         >  1:1176139/1‑69 (MQ=255)
         aTCATATGATGACGGCGACGCTGCAAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGAATGTCAAAc                                                         >  1:117827/1‑69 (MQ=255)
         aTCATAGGATGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGAATGTCAAAc                                                         >  1:440321/1‑69 (MQ=255)
           tatatGGTGACGGAGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGGATGTCGAACAg                                                       >  1:1912942/2‑69 (MQ=255)
           cATATGGTGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGGATGTCGAACAg                                                       >  1:1673389/1‑69 (MQ=255)
           cATATGGTGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGGATGTCGAACAg                                                       >  1:243935/1‑69 (MQ=255)
              atGGTGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGGATGTCGAACAgc                                                      >  1:2026723/1‑67 (MQ=255)
              atGGTGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGGATGTCGAACAgc                                                      >  1:2028223/1‑67 (MQ=255)
              atGGTGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGGATGTCGAACAgc                                                      >  1:2244507/1‑67 (MQ=255)
              atGGTGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGGATGTCGAACAgc                                                      >  1:178561/1‑67 (MQ=255)
              atGGTGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGGATGTCGAACAgc                                                      >  1:1697797/1‑67 (MQ=255)
              atGGTGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGGATGTCGAACAgc                                                      >  1:1221748/1‑67 (MQ=255)
              atGGTGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGGATGTCGAACAgc                                                      >  1:718485/1‑67 (MQ=255)
              atGGTGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGGATGTCGAACAgc                                                      >  1:1546710/1‑67 (MQ=255)
               tGGTGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGGATGTCGAACAGCGcc                                                   >  1:2072022/1‑69 (MQ=255)
               tGGTGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGGATGTCGAACAGCGcc                                                   >  1:1113745/1‑69 (MQ=255)
               tGGTGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGGATGTCGAACAGCGcc                                                   >  1:3230435/1‑69 (MQ=255)
                 gTGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGGATGTCGAACAGCGcc                                                   >  1:713278/1‑67 (MQ=255)
                   gACGGCGACGCTGCCAGGAGAGACGCAAACGTGG‑CAGAGGATTTTCGGGATGTCGAACAGCGCcagcag                                              <  1:2433765/69‑1 (MQ=255)
                    actgCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGAATGTCAAACAGCGCcagcag                                              <  1:1683854/66‑1 (MQ=255)
                    aCGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGGATGTCGAACAGCGCcagcag                                              <  1:914342/69‑1 (MQ=255)
                    aCGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGGATGTCGAACAGCGCcagcag                                              <  1:1223797/69‑1 (MQ=255)
                    aCGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGAATGTCAAACAGCGCcagcag                                              <  1:2024159/69‑1 (MQ=255)
                    aCGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGAATGTCAAACAGCGCcagcag                                              <  1:127603/69‑1 (MQ=255)
                    aCGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGAATGTCAAACAGCGCcagcag                                              <  1:2143290/69‑1 (MQ=255)
                    aCGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGAATGTCAAACAGCGCcagcag                                              <  1:3167279/69‑1 (MQ=255)
                    aCGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGAATGTCAAACAGCGCcagcag                                              <  1:1236949/69‑1 (MQ=255)
                    aCGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGAATGTCAAACAGCGCcagcag                                              <  1:1719247/69‑1 (MQ=255)
                    aCGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGAATGTCAAACAGCGCcagcag                                              <  1:2411637/69‑1 (MQ=255)
                     cGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGAATGTCAAACAGCGCcagcag                                              <  1:1573374/68‑1 (MQ=255)
                     cGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGAATGTCAAACAGCGCcagcag                                              <  1:3178709/68‑1 (MQ=255)
                     cGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGAATGTCAAACAGCGCcagcag                                              <  1:2759977/68‑1 (MQ=255)
                           cGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGAATGTCAAACAGCGCcagcag                                              <  1:616133/62‑1 (MQ=255)
                             cTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGGATGTCGAACAGCGCCAGCAGGTTGTCATc                                     <  1:1403541/69‑1 (MQ=255)
                             cTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGGATGTCGAACAGCGCCAGCAGGTTGTCATc                                     <  1:2677640/69‑1 (MQ=255)
                             cTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGGATGTCGAACAGCGCCAGCAGGTTGTCATc                                     <  1:173629/69‑1 (MQ=255)
                             cTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGGATGTCGAACAGCGCCAGCAGGTTGTCATc                                     <  1:1213053/69‑1 (MQ=255)
                             cTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGGATGTCGAACAGCGCCAGCAGGTTGTCATc                                     <  1:73181/69‑1 (MQ=255)
                             cTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGGATGTCGAACAGCGCCAGCAGGTTGTCATc                                     <  1:16570/69‑1 (MQ=255)
                              tGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGGATGTCGAACAGCGCCAGCAGGTTGTCATCt                                    <  1:741178/69‑1 (MQ=255)
                              tGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGGATGTCGAACAGCGCCAGCAGGTTGTCATCt                                    <  1:3000952/69‑1 (MQ=255)
                                          cGCAAACGTGGCCAGAGGATTTTCGGAATGTCAAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTg                        <  1:2448416/69‑1 (MQ=255)
                                          cGCAAACGTGGCCAGAGGATTTTCGGAATGTCAAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTg                        <  1:673733/69‑1 (MQ=255)
                                          cGCAAACGTGGCCAGAGGATTTTCGGAATGTCAAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTg                        <  1:402766/69‑1 (MQ=255)
                                          cGCAAACGTGGCCAGAGGATTTTCGGAATGTCAAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTg                        <  1:385770/69‑1 (MQ=255)
                                          cGCAAACGTGGCCAGAGGATTTTCGGAATGTCAAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTg                        <  1:368401/69‑1 (MQ=255)
                                          cGCAAACGTGGCCAGAGGATTTTCGGAATGTCAAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTg                        <  1:931025/69‑1 (MQ=255)
                                          cGCAAACGTGGCCAGAGGATTTTCGGAATGTCAAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTg                        <  1:1718118/69‑1 (MQ=255)
                                          cGCAAACGTGGCCAGAGGATTTTCGGAATGTCAAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTg                        <  1:2930415/69‑1 (MQ=255)
                                          cGCAAACGTGGCCAGAGGATTTTCGGAATGTCAAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTg                        <  1:232441/69‑1 (MQ=255)
                                          cGCAAACGTGGCCAGAGGATTTTCGGAATGTCAAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTg                        <  1:2713314/69‑1 (MQ=255)
                                           gCAAACGTGGCCAGAGGATTTTCGGAATGTCAAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTg                        <  1:798580/68‑1 (MQ=255)
                                                   ggCCAGAGGATTTTCGGAATGTCAAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTg                        <  1:648232/60‑1 (MQ=255)
                                                       agagGATTTTCGGGATGTCGAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTGTCGGTTGCGTGaa           <  1:517653/69‑1 (MQ=255)
                                                       agagGATTTTCGGGATGTCGAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTGTCGGTTGCGTGaa           <  1:711995/69‑1 (MQ=255)
                                                       agagGATTTTCGGGATGTCGAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTGTCGGTTGCGTGaa           <  1:377723/69‑1 (MQ=255)
                                                       agagGATTTTCGGGATGTCGAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTGTCGGTTGCGTGaa           <  1:2472643/69‑1 (MQ=255)
                                                         agGATTTTCGGGATGTCGAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTGTCGGTTGCGTGAAGa         <  1:1884461/69‑1 (MQ=255)
                                                         agGATTTTCGGGATGTCGAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTGTCGGTTGCGTGAAGa         <  1:3290138/69‑1 (MQ=255)
                                                          ggATTTTCGGGATGTCGAACAGTGCCAGCAGGTTGTCATCTTTCAGCACCTTGTCGGTTGCGTGAAGa         >  1:2844439/1‑68 (MQ=255)
                                                          ggATTTTCGGGATGTCGAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTGTCGGTTGCGTGAAGa         >  1:1880038/1‑68 (MQ=255)
                                                          ggATTTTCGGGATGTCGAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTGTCGGTTGCGTGAAGa         >  1:2149814/1‑68 (MQ=255)
                                                          ggATTTTCGGAATGTCAAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTGTCGGTGGCGTGAAGa         >  1:813206/1‑68 (MQ=255)
                                                          ggATTTTCGGAATGTCAAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTGTCGGTGGCGTGAAGa         >  1:2047401/1‑68 (MQ=255)
                                                          ggATTTTCGGAATGTCAAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTGTCGGTGGCGTGAAGa         >  1:1675583/1‑68 (MQ=255)
                                                          ggATTTTCGGAATGTCAAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTGTCGGTGGCGTGAAGa         >  1:1786702/1‑68 (MQ=255)
                                                          ggATTTTCGGAATGTCAAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTGTCGGTGGCGTGAAGa         >  1:2175525/1‑68 (MQ=255)
                                                          ggATTTTCGGAATGTCAAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTGTCGGTGGCGTGAAGa         >  1:2159196/1‑68 (MQ=255)
                                                            aTTTTCGGGATGTCGAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTGTCGGTTGCGTGAAGATAc      <  1:456162/69‑1 (MQ=255)
                                                            aTTTTCGGGATGTCGAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTGTCGGTTGCGTGAAGATAc      <  1:69413/69‑1 (MQ=255)
                                                            aTTTTCGGGATGTCGAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTGTCGGTTGCGTGAAGATAc      <  1:3219157/69‑1 (MQ=255)
                                                            aTTTTCGGGATGTCGAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTGTCGGTTGCGTGAAGATAc      <  1:1706787/69‑1 (MQ=255)
                                                            aTTTTCGGGATGTCGAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTGTCGGTTGCGTGAAGATAc      <  1:2788911/69‑1 (MQ=255)
                                                            aTTTTCGGGATGTCGAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTGTCGGTTGCGTGAAGATAc      <  1:2071786/69‑1 (MQ=255)
                                                             ttttCGGGATGTCGAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTGTCGGTTGCGTGAAGATAc      <  1:50546/68‑1 (MQ=255)
                                                             ttttCGGGATGTCGAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTGTCGGTTGCGTGAAGATAc      <  1:3106407/68‑1 (MQ=255)
                                                                 cGGGATGTCGAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTGTCGGTTGCGTGAAGATACATCa  <  1:468608/68‑1 (MQ=255)
                                                                 cGGGATGTCGAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTGTCGGTTGCGTGAAGATACATCa  <  1:963303/68‑1 (MQ=255)
                                                                 cGGGATGTCGAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTGTCGGTTGCGTGAAGATACATCa  <  1:1963391/68‑1 (MQ=255)
                                                                 cGGGATGTCGAACAGCGCCAGCAGGTTGGCATCTTTCAGCACCTTGTCGGTTGCGTGAAGATACATCa  <  1:1187539/68‑1 (MQ=255)
                                                                    gATGTCGAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTGTCGGTTGCGTGAAGATAc      <  1:1562094/61‑1 (MQ=255)
                                                                    |                                                                
CGACCAGTGATCATATGGTGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGATTTTCGGGATGTCGAACAGCGCCAGCAGGTTGTCATCTTTCAGCACCTTGTCGGTTGCGTGAAGATACATCA  >  minE/2000336‑2000468

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A