Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,001,058 |
A→G |
44.4% |
F75F (TTT→TTC) |
gss ← |
fused glutathionylspermidine amidase and glutathionylspermidine synthetase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,001,058 | 0 | A | G | 44.4%
| 4.5
/ 34.3
| 45 | F75F (TTT→TTC) | gss | fused glutathionylspermidine amidase and glutathionylspermidine synthetase |
| Reads supporting (aligned to +/- strand): ref base A (14/11); new base G (7/13); total (21/24) |
| Fisher's exact test for biased strand distribution p-value = 2.31e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.67e-01 |
TTAACCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGCCATACCCACGTCAGTAAAGACCACACCGTAATTCAGAAAGAGAAAACGGCGAGCAAATTCAACGCATTGCCAC > minE/2000999‑2001115
|
ttgaCCACTTCACGCAGGAAGCGCAGTGAGAAAATCTCCCATGCCATACCCATGTCAGTGAAGACtaca > 1:2939684/4‑69 (MQ=255)
ttgaCCACTTCACGCAGGAAGCGCAGTGAGAAAATCTCCCATGCCATACCCACGTCAGTGAAGACtaca > 1:862634/4‑69 (MQ=255)
ttgaCCACTTCACGCAGGAAGCGCAGTGAGAAAATCTCCCATGCCATACCCACGTCAGTGAAGACtaca > 1:639275/4‑69 (MQ=255)
ttgaCCACTTCACGCAGGAAGCGCAGTGAGAAAATCTCCCATGCCATACCCACGTCAGTGAAGACtaca > 1:571920/4‑69 (MQ=255)
ttgaCCACTTCACGCAGGAAGCGCAGTGAGAAAATCTCCCATGCCATACCCACGTCAGTGAAGACtaca > 1:1632361/4‑69 (MQ=255)
ttgaCCACTTCACGCAGGAAGCGCAGTGAGAAAATCTCCCATGCCATACCCACGTCAGTGAAGACtaca > 1:3115607/4‑69 (MQ=255)
ttgaCCACTTCACGCAGGAAGCGCAGTGAGAAAATCTCCCATGCCATACCCACGTCAGTGAAGACtaca > 1:345760/4‑69 (MQ=255)
aaCCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGCCATACCCACGTCAGTAAAGACCacac > 1:1086433/1‑68 (MQ=255)
aaCCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGCCATACCCACGTCAGTAAAGACCacac > 1:1958547/1‑68 (MQ=255)
aaCCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGCCATACCCACGTCAGTAAAGACCacac > 1:3186526/1‑68 (MQ=255)
aaCCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGCCATACCCACGTCAGTAAAGACCacac > 1:1772547/1‑68 (MQ=255)
cGCAGGAAGCGCAGCGAGAAAATCTCCCACGCCATACCCACGTCAGTAAAGACCACACCGTAATTCa < 1:927569/67‑1 (MQ=255)
cGCAGGAAGCGCAGCGAGAAAATCTCCCACGCCATACCCACGTCAGTAAAGACCACACCGTAATTCa < 1:2064612/67‑1 (MQ=255)
gAAGCGCAGCGAGAAAATCTCCCACGCCATACCCACGTCAGTAAAGACCACACCGTAATTCAGAAaga > 1:2506944/1‑68 (MQ=255)
aaGCGCAGCGAGAAAATCTCCCACGCCATACCCACGTCAGTAAAGACCACACCGTAATTCAGAAagag > 1:1721002/1‑68 (MQ=255)
aaGCGCAGCGAGAAAATCTCCCACGCCATACCCACGTCAGTAAAGACCACACCGTAATTCAGAAagag > 1:13784/1‑68 (MQ=255)
aaGCGCAGCGAGAAAATCTCCCACGCCATACCCACGTCAGTAAAGACCACACCGTAATTCAGAAagag > 1:2108029/1‑68 (MQ=255)
gaAAATCTCCCATGCCATACCCACGTCAGTGAAGACTACACCGTAGTTCAGAAAGAGaa < 1:497315/59‑1 (MQ=255)
gaAAATCTCCCATGCCATACCCACGTCAGTGAAGACTACACCGTAGTTCAGAAAGAGaa < 1:687833/59‑1 (MQ=255)
gaAAATCTCCCATGCCATACCCACGTCAGTGAAGACTACACCGTAGTTCAGAAAGAGaa < 1:30902/59‑1 (MQ=255)
gaAAATCTCCCATGCCATACCCACGTCAGTGAAGACTACACCGTAGTTCAGAAAGAGaa < 1:1044477/59‑1 (MQ=255)
gaAAATCTCCCATGCCATACCCACGTCAGTGAAGACTACACCGTAGTTCAGAAAGAGaa < 1:2542025/59‑1 (MQ=255)
gaAAATCTCCCATGCCATACCCACGTCAGTGAAGACTACACCGTAGTTCAGAAAGAGaa < 1:965294/59‑1 (MQ=255)
gaAAATCTCCCATGCCATACCCACGTCAGTGAAGACTACACCGTAGTTCAGAAAGAGaa < 1:1779516/59‑1 (MQ=255)
gaAAATCTCCCATGCCATACCCACGTCAGTGAAGACTACACCGTAGTTCAGAAAGAGaa < 1:1092644/59‑1 (MQ=255)
gaAAATCTCCCATGCCATACCCACGTCAGTGAAGACTACACCGTAGTTCAGAAAGAGaa < 1:1226896/59‑1 (MQ=255)
gaAAATCTCCCATGCCATACCCACGTCAGTGAAGACTACACCGTAGTTCAGAAAGAGaa < 1:1278680/59‑1 (MQ=255)
gaAAATCTCCCATGCCATACCCACGTCAGTGAAGACTACACCGTAGTTCAGAAAGAGaa < 1:1828098/59‑1 (MQ=255)
gaAAATCTCCCATGCCATACCCACGTCAGTGAAGACTACACCGTAGTTCAGAAAGAGaa < 1:2456589/59‑1 (MQ=255)
gaAAATCTCCCATGCCATACCCACATCAGTGAAGACTACACCGTAGTTCAGAAAGAGaa < 1:500366/59‑1 (MQ=255)
aCGCCATACCCACGTCAGTAAAGACCACACCGTAATTCAGAAAGAGAAAACGGCGAGCAAATTCAACg < 1:2075809/68‑1 (MQ=255)
aCGCCATACCCACGTCAGTAAAGACCACACCGTAATTCAGAAAGAGAAAACGGCGAGCAAATTCAACg < 1:2591575/68‑1 (MQ=255)
aCGCCATACCCACGTCAGTAAAGACCACACCGTAATTCAGAAAGAGAAAACGGCGAGCAAATTCAACg < 1:1257385/68‑1 (MQ=255)
aCGCCATACCCACGTCAGTAAAGACCACACCGTAATTCAGAAAGAGAAAACGGCGAGCAAATTCAACg < 1:1471895/68‑1 (MQ=255)
aCGCCATACCCACGTCAGTAAAGACCACACCGTAATTCAGAAAGAGAAAACGGCGAGCAAATTCAACg < 1:365229/68‑1 (MQ=255)
aCGCCATACCCACGTCAGTAAAGACCACACCGTAATTCAGAAAGAGAAAACGGCGAGCAAATTCAACg < 1:3204295/68‑1 (MQ=255)
aCGCCATACCCACGTCAGTAAAGACCACACCGTAATTCAGAAAGAGAAAACGGCGAGCAAATTCAACg < 1:2159627/68‑1 (MQ=255)
aCGCCATACCCACGTCAGTAAAGACCACACCGTAATTCAGAAAGAGAAAACGGCGAGCAAATTCAACg < 1:3101310/68‑1 (MQ=255)
aCGCCATACCCACGTCAGTAAAGACCACACCGTAATTCAGAAAGAGAAAACGGCGAGCAAATTCAACg < 1:2972366/68‑1 (MQ=255)
cccACGTCAGTAAAGACCACACCGTAATTCAGAAAGAGAAAACGGCGATCAAATTCAACg > 1:2479479/1‑60 (MQ=255)
cccACGTCAGTAAAGACCACACCGTAATTCAGAAAGAGAAAACGGCGAGCa > 1:2717520/1‑51 (MQ=255)
cccACGTCAGTAAAGACCACACCGTAATTCAGAAAGAGAAAACGGCGAGCa > 1:1101270/1‑51 (MQ=255)
cccACGTCAGTAAAGACCACACCGTAATTCAGAAAGAGAAAACGGCGAGCAAATTCAACg > 1:1683298/1‑60 (MQ=255)
cccACGTCAGTAAAGACCACACCGTAATTCAGAAAGAGAAAACGGCGAGCAAATTCAACg > 1:6277/1‑60 (MQ=255)
cccACGTCAGTAAAGACCACACCGTAATTCAGAAAGAGAAAACGGCGAGCAAATTCAACGCATTGCCAc > 1:1796196/1‑69 (MQ=255)
|
TTAACCACTTCTCGCAGGAAGCGCAGCGAGAAAATCTCCCACGCCATACCCACGTCAGTAAAGACCACACCGTAATTCAGAAAGAGAAAACGGCGAGCAAATTCAACGCATTGCCAC > minE/2000999‑2001115
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
