Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,012,911 |
C→T |
33.6% |
L164L (CTG→TTG) |
yghA → |
predicted glutathionylspermidine synthase, with NAD(P)‑binding Rossmann‑fold domain |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,012,911 | 0 | C | T | 33.6%
| 69.3
/ 45.2
| 86 | L164L (CTG→TTG) | yghA | predicted glutathionylspermidine synthase, with NAD(P)‑binding Rossmann‑fold domain |
| Reads supporting (aligned to +/- strand): ref base C (39/18); new base T (12/17); total (51/35) |
| Fisher's exact test for biased strand distribution p-value = 2.08e-02 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01 |
CATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCAC > minE/2012844‑2012972
|
cATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCt > 1:3341805/1‑69 (MQ=255)
cATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCt > 1:1300464/1‑69 (MQ=255)
ttCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGt < 1:3408614/69‑1 (MQ=255)
ttCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGt < 1:3010896/69‑1 (MQ=255)
tCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGt < 1:1210645/68‑1 (MQ=255)
tCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGt < 1:1631272/68‑1 (MQ=255)
ggATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCt < 1:872514/68‑1 (MQ=255)
gCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTaa > 1:411891/1‑67 (MQ=255)
gCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTaa > 1:1970761/1‑67 (MQ=255)
aCCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCg > 1:3126078/1‑69 (MQ=255)
aCCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCg > 1:2980673/1‑69 (MQ=255)
aCCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCg > 1:1900478/1‑69 (MQ=255)
aCCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCg > 1:1875103/1‑69 (MQ=255)
aCCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCg > 1:1730053/1‑69 (MQ=255)
aCCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCg > 1:2283212/1‑69 (MQ=255)
aCCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCg > 1:2442040/1‑69 (MQ=255)
aCCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCg > 1:2568658/1‑69 (MQ=255)
aCCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCg > 1:2678366/1‑69 (MQ=255)
aCCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCg > 1:1539522/1‑69 (MQ=255)
aCCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCg > 1:832544/1‑69 (MQ=255)
aCCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCg > 1:600792/1‑69 (MQ=255)
aCCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCg > 1:637769/1‑69 (MQ=255)
aCCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCg > 1:1387217/1‑69 (MQ=255)
aGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATc > 1:1534279/1‑69 (MQ=255)
aGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATc > 1:1702671/1‑69 (MQ=255)
tCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGt > 1:1388926/1‑35 (MQ=255)
tCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGt > 1:2300724/1‑35 (MQ=255)
tCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGt > 1:2840859/1‑35 (MQ=255)
tCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGt > 1:2818717/1‑35 (MQ=255)
tCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGt > 1:3135757/1‑35 (MQ=255)
tCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGt > 1:3204151/1‑35 (MQ=255)
tCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGt > 1:1779652/1‑35 (MQ=255)
tCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGt > 1:2789329/1‑35 (MQ=255)
aaGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGaa < 1:2850905/68‑1 (MQ=255)
aGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAg > 1:3072737/1‑69 (MQ=255)
aGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAg > 1:2148636/1‑69 (MQ=255)
aGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAg > 1:2215776/1‑69 (MQ=255)
aGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAg > 1:470506/1‑69 (MQ=255)
cTTTGCCATTAACGTTTTCGCGTTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGc < 1:2646024/69‑1 (MQ=255)
cTTTGCCATTAACGTTTTCGCGTTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGc < 1:863581/69‑1 (MQ=255)
cTTTGCCATTAACGTTTTCGCGTTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGc < 1:856740/69‑1 (MQ=255)
cTTTGCCATTAACGTTTTCGCGTTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGc < 1:694665/69‑1 (MQ=255)
cTTTGCCATTAACGTTTTCGCGTTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGc < 1:678790/69‑1 (MQ=255)
cTTTGCCATTAACGTTTTCGCGTTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGc < 1:3037357/69‑1 (MQ=255)
cTTTGCCATTAACGTTTTCGCGTTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGc < 1:487975/69‑1 (MQ=255)
cTTTGCCATTAACGTTTTCGCGTTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGc < 1:3274712/69‑1 (MQ=255)
cTTTGCCATTAACGTTTTCGCGTTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGc < 1:1431761/69‑1 (MQ=255)
cTTTGCCATTAACGTTTTCGCGTTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGc < 1:1062527/69‑1 (MQ=255)
cTTTGCCATTAACGTTTTCGCGTTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGc < 1:1262882/69‑1 (MQ=255)
cTTTGCCATTAACGTTTTCGCGTTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGc < 1:190913/69‑1 (MQ=255)
tttGCCATTAACGTTTTCGCGTTGTTCTGGCTACCCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGc < 1:1851291/68‑1 (MQ=255)
tttGCCATTAACGTTTTCGCGTTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGc < 1:2821821/68‑1 (MQ=255)
tttGCCATTAACGTTTTCGCGTTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGc < 1:1603396/68‑1 (MQ=255)
tttGCCATTAACGTTTTCGCGTTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGc < 1:1672807/68‑1 (MQ=255)
ccATTAACGTTTTCGCGTTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCCAAAGGTGc < 1:2081229/64‑1 (MQ=255)
ccATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAg < 1:1667477/67‑1 (MQ=255)
cATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTa < 1:582285/68‑1 (MQ=255)
cATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTa < 1:373478/68‑1 (MQ=255)
aTTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTat < 1:622693/68‑1 (MQ=255)
aTTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTat < 1:164704/68‑1 (MQ=255)
aTTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTat < 1:2102677/68‑1 (MQ=255)
aTTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTat < 1:2234351/68‑1 (MQ=255)
aTTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTat < 1:2582478/68‑1 (MQ=255)
aTTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTat < 1:2655523/68‑1 (MQ=255)
aTTAACGTTTTCGCGCTGTTCTGGCTAACACAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTa < 1:3073649/67‑1 (MQ=255)
aaCGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGc > 1:1357016/1‑46 (MQ=255)
aaCGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGc > 1:345748/1‑46 (MQ=255)
aaCGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTatcatc > 1:2023554/1‑69 (MQ=255)
aaCGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTatcatc > 1:1168835/1‑69 (MQ=255)
aaCGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTatcatc > 1:381713/1‑69 (MQ=255)
aaCGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTatcatc > 1:62380/1‑69 (MQ=255)
aaCGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTatcatc > 1:2670414/1‑69 (MQ=255)
gTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATcacc < 1:3132614/69‑1 (MQ=255)
gTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCCGCTACCGAAAGGTGCAAGTATCATcacc < 1:1339322/69‑1 (MQ=255)
tttCGCGTTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATcaccac > 1:674098/1‑69 (MQ=255)
tttCGCGTTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATcaccac > 1:2729118/1‑69 (MQ=255)
tttCGCGTTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATcaccac > 1:1396729/1‑69 (MQ=255)
tttCGCGTTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATcaccac > 1:1296390/1‑69 (MQ=255)
tttCGCGTTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATcaccac > 1:1463924/1‑69 (MQ=255)
tttCGCGTTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATcaccac > 1:371091/1‑69 (MQ=255)
tttCGCGTTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATcaccac > 1:3205015/1‑69 (MQ=255)
tttCGCGTTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATcaccac > 1:199638/1‑69 (MQ=255)
tttCGCGTTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATcaccac > 1:2882929/1‑69 (MQ=255)
tttCGCGTTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATcaccac > 1:2345894/1‑69 (MQ=255)
tttCGCGTTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATcacca > 1:1405135/1‑68 (MQ=255)
tttCGCGTTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATcacca > 1:338839/1‑68 (MQ=255)
gcgtTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAACGTGc < 1:1278906/50‑1 (MQ=255)
|
CATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCAC > minE/2012844‑2012972
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
