Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,014,583 |
G→A |
43.2% |
R54R (CGC→CGT) |
exbB ← |
membrane spanning protein in TonB‑ExbB‑ExbD complex |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,014,583 | 0 | G | A | 43.2%
| 32.7
/ 79.3
| 104 | R54R (CGC→CGT) | exbB | membrane spanning protein in TonB‑ExbB‑ExbD complex |
| Reads supporting (aligned to +/- strand): ref base G (20/39); new base A (13/32); total (33/71) |
| Fisher's exact test for biased strand distribution p-value = 6.73e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
Only 100 of 104 total aligned reads displayed.
GATATCGTTGGCCTGGTTTAAGGAACGCGCTTCAGCCAGCAGTTGCTGCTCGCGCTTAAGGCGACGCTTCTGATTGAAGAACTCTACGCTCTTACTGAAGAAGATTGCCCAGGTGACTACGGAG > minE/2014523‑2014646
|
gATATCGTTGGCCTGGTTTAAGGAACGCGCTTCAGCCAGCAGTTGCTGGTCGCGCTTAAGGCGACGCtt > 1:481791/1‑69 (MQ=255)
gATATCGTTGGCCTGGTTTAAGGAACGCGCTTCAGCCAGCAGTTGCTGCTCGCGCTTAAGGCGACGCtt > 1:1114565/1‑69 (MQ=255)
gATATCGTTGGCCTGGTTTAAGGAACGCGCTTCAGCCAGCAGTTGCTGCTCGCGCTTAAGGCGACGCtt > 1:2898717/1‑69 (MQ=255)
tatCGTTGGCCTGGTTTAAGGAACGCGCTTCAGCCAGCAGTTGCTGCTCGCGCTTAAGGCGACGCTTCt < 1:285636/69‑1 (MQ=255)
tatCGTTGGCCTGGTTTAAGGAACGCGCTTCAGCCAGCAGTTGCTGCTCGCGCTTAAGGCGACGCTTCt < 1:2139359/69‑1 (MQ=255)
ttAAGGAACGCGCTTCAGCCAGCAGTTGCTGCTCGCGCTTAAGGCGACGCTTCTGATTGAAGAActc < 1:1210924/67‑1 (MQ=255)
ttAAGGAACGCGCTTCAGCCAGCAGTTGCTGCTCGCGCTTAAGGCGACGCTTCTGATTGAAGAActc < 1:3279886/67‑1 (MQ=255)
ttAAGGAACGCGCTTCAGCCAGCAGTTGCTGCTCGCGCTTAAGGCGACGCTTCTGATTGAAGAActc < 1:2338506/67‑1 (MQ=255)
ttAAGGAACGCGCTTCAGCCAGCAGTTGCTGCTCGCGCTTAAGGCGACGCTTCTGATTGAAGAActc < 1:912877/67‑1 (MQ=255)
ttAAGGAACGCGCTTCAGCCAGCAGTTGCTGCTCGCGCTTAAGGCGACGCTTCTGATTGAAGAActc < 1:3186864/67‑1 (MQ=255)
ttAAGGAACGCGCTTCAGCCAGCAGTTGCTGCTCGCGCTTAAGGCGACGCTTCTGATTGAAGAActc < 1:476836/67‑1 (MQ=255)
aaCGCGCTTCAGCCAGCAGTTGCTGCTCGCGCTTAAGGCGACGCTTCTGATTGAAGAACTCTACGctct < 1:2832628/69‑1 (MQ=255)
aaCGCGCTTCAGCCAGCAGTTGCTGCTCGCGCTTAAGGCGACGCTTCTGATTGAAGAACTCTACGctct < 1:467787/69‑1 (MQ=255)
aaCGCGCTTCAGCCAGCAGTTGCTGCTCGCGCTTAAGGCGACGCTTCTGATTGAAGAACTCTACGctct < 1:2325246/69‑1 (MQ=255)
aCGCGCTTCAGCCAGCAGTTGCTGCTCGCGCTTAAGGCGACGCTTCTGATTGAAGAACTCTACGCTCtt > 1:804914/1‑69 (MQ=255)
cagccagcAGTTGCTGCTCGCGCTTAAGACGACGCTTCTGATTGAAGAACTCCACGCTCTTACTga > 1:3212675/1‑66 (MQ=255)
cagccagcAGTTGCTGCTCGCGCTTAAGACGACGCTTCTGATTGAAGAACTCCACGCTCTTACTga > 1:410608/1‑66 (MQ=255)
cagccagcAGTTGCTGCTCGCGCTTAAGACGACGCTTCTGATTGAAGAACTCCACGCTCTTACTga > 1:2820659/1‑66 (MQ=255)
cagccagcAGTTGCTGCTCGCGCTTAAGACGACGCTTCTGATTGAAGAACTCCACGCTCTTACTga > 1:501398/1‑66 (MQ=255)
cagccagcAGTTGCTGCTCGCGCTTAAGACGACGCTTCTGATTGAAGAACTCCACGCTCTTACTga > 1:512382/1‑66 (MQ=255)
cagccagcAGTTGCTGCTCGCGCTTAAGACGACGCTTCTGATTGAAGAACTCCACGCTCTTACTga > 1:2556326/1‑66 (MQ=255)
cagccagcAGTTGCTGCTCGCGCTTAAGACGACGCTTCTGATTGAAGAACTCCACGCTCTTACTga > 1:567286/1‑66 (MQ=255)
cagccagcAGTTGCTGCTCGCGCTTAAGACGACGCTTCTGATTGAAGAACTCCACGCTCTTACTga > 1:654356/1‑66 (MQ=255)
cagccagcAGTTGCTGCTCGCGCTTAAGACGACGCTTCTGATTGAAGAACTCCACGCTCTTACTga > 1:2194435/1‑66 (MQ=255)
cagccagcAGTTGCTGCTCGCGCTTAAGACGACGCTTCTGATTGAAGAACTCCACGCTCTTACTga > 1:465832/1‑66 (MQ=255)
cagccagcAGTTGCTGCTCGCGCTTAAGACGACGCTTCTGATTGAAGAACTCCACGCTCTTACTga > 1:2038047/1‑66 (MQ=255)
cagccagcAGTTGCTGCTCGCGCTTAAGACGACGCTTCTGATTGAAGAACTCCACGCTCTTACTga > 1:1815074/1‑66 (MQ=255)
gccagcAGTTGCTGCTCGCGCTTAAGGCGACGCTTCTGATTGAAGAACTCTACGCTCTTACTgaagaa < 1:2083515/68‑1 (MQ=255)
gccagcAGTTGCTGCTCGCGCTTAAGGCGACGCTTCTGATTGAAGAACTCTACGCTCTTACTgaagaa < 1:3128304/68‑1 (MQ=255)
gccagcAGTTGCTGCTCGCGCTTAAGGCGACGCTTCTGATTGAAGAACTCTACGCTCTTACTgaagaa < 1:11357/68‑1 (MQ=255)
gccagcAGTTGCTGCTCGCGCTTAAGGCGACGCTTCTGATTGAAGAACTCTACGCTCTTACTgaagaa < 1:3266783/68‑1 (MQ=255)
gccagcAGTTGCTGCTCGCGCTTAAGGCGACGCTTCTGATTGAAGAACTCTACGCTCTTACTgaagaa < 1:347442/68‑1 (MQ=255)
gccagcAGTTGCTGCTCGCGCTTAAGGCGACGCTTCTGATTGAAGAACTCTACGCTCTTACTgaagaa < 1:835888/68‑1 (MQ=255)
gccagcAGTTGCTGCTCGCGCTTAAGGCGACGCTTCTGATCGAAGAACTCTACGCTCTTACTgaagaa < 1:1909071/68‑1 (MQ=255)
ccagcAGTTGCTGCTCGCGCTTAAGGCGACGCTTCTGATTGAAGAACTCTACGCTCTTACTGaagaag < 1:1837128/68‑1 (MQ=255)
ccagcAGTTGCTGCTCGCGCTTAAGGCGACGCTTCTGATTGAAGAACTCTACGCTCTTACTGaagaag < 1:1115103/68‑1 (MQ=255)
ccagcAGTTGCTGCTCGCGCTTAAGGCGACGCTTCTGATTGAAGAACTCTACGCTCTTACTGaagaag < 1:122761/68‑1 (MQ=255)
ccagcAGTTGCTGCTCGCGCTTAAGGCGACGCTTCTGATTGAAGAACTCTACGCTCTTACTGaagaag < 1:3341580/68‑1 (MQ=255)
ccagcAGTTGCTGCTCGCGCTTAAGGCGACGCTTCTGATTGAAGAACTCTACGCTCTTACTGaagaag < 1:1532221/68‑1 (MQ=255)
ccagcAGTTGCTGCTCGCGCTTAAGGCGACGCTTCTGATTGAAGAACTCTACGCTCTTACTGaagaag < 1:2811025/68‑1 (MQ=255)
ccagcAGTTGCTGCTCGCGCTTAAGGCGACGCTTCTGATTGAAGAACTCTACGCTCTTACTGaagaag < 1:2282383/68‑1 (MQ=255)
ccagcAGTTGCTGCTCGCGCTTAAGGCGACGCTTCTGATTGAAGAACTCTACGCTCTTACTGaagaag < 1:1934645/68‑1 (MQ=255)
cagcagTTGCTGCTCGCGCTTAAGGCGACGCTTCTGATTgaag > 1:2340690/1‑43 (MQ=255)
cagcagTTGCTGCTCGCGCTTAAGGCGACGCTTCTGATTgaag > 1:387281/1‑43 (MQ=255)
cagcagTTGCTGCTCGCGCTTAAGGCGACGCTTCTGATTgaag > 1:199851/1‑43 (MQ=255)
cagcagTTGCTGCTCGCGCTTAAGGCGACGCTTCTGATTgaag > 1:2856405/1‑43 (MQ=255)
cagcagTTGCTGCTCGCGCTTAAGGCGACGCTTCTGATTgaag > 1:3263785/1‑43 (MQ=255)
cagcagTTGCTGCTCGCGCTTAAGGCGACGCTTCTGATTgaag > 1:1019327/1‑43 (MQ=255)
cagcagTTGCTGCTCGCGCTTAAGGCGACGCTTCTGATTGAAGAACTCTACGCTCTTACTGAAGAAGAt < 1:1881443/69‑1 (MQ=255)
cagcagTTGCTGCTCGCGCTTAAGGCGACGCTTCTGATTGAAGAACTCTACGCTCTTACTGAAGAAGAt < 1:1763613/69‑1 (MQ=255)
cagcagTTGCTGCTCGCGCTTAAGGCGACGCTTCTGATTGAAGAACTCTACGCTCTTACTGAAGAAGAt < 1:2326508/69‑1 (MQ=255)
gTTGCTGCTCGCGCTTAAGACGACGCTTCTGATTGAAGAACTCCACGCTCTTACTGAAGAAGATTGccc < 1:2161221/69‑1 (MQ=255)
gTTGCTGCTCGCGCTTAAGACGACGCTTCTGATTGAAGAACTCCACGCTCTTACTGAAGAAGATTGccc < 1:734079/69‑1 (MQ=255)
gTTGCTGCTCGCGCTTAAGACGACGCTTCTGATTGAAGAACTCCACGCTCTTACTGAAGAAGATTGccc < 1:828943/69‑1 (MQ=255)
gTTGCTGCTCGCGCTTAAGACGACGCTTCTGATTGAAGAACTCCACGCTCTTACTGAAGAAGATTGccc < 1:3227497/69‑1 (MQ=255)
gTTGCTGCTCGCGCTTAAGACGACGCTTCTGATTGAAGAACTCCACGCTCTTACTGAAGAAGATTGccc < 1:439675/69‑1 (MQ=255)
gTTGCTGCTCGCGCTTAAGACGACGCTTCTGATTGAAGAACTCCACGCTCTTACTGAAGAAGATTGccc < 1:1089154/69‑1 (MQ=255)
gTTGCTGCTCGCGCTTAAGACGACGCTTCTGATTGAAGAACTCCACGCTCTTACTGAAGAAGATTGccc < 1:2776336/69‑1 (MQ=255)
gTTGCTGCTCGCGCTTAAGACGACGCTTCTGATTGAAGAACTCCACGCTCTTACTGAAGAAGATTGccc < 1:1801152/69‑1 (MQ=255)
gTTGCTGCTCGCGCTTAAGACGACGCTTCTGATTGAAGAACTCCACGCTCTTACTGAAGAAGATTGccc < 1:2621651/69‑1 (MQ=255)
gTTGCTGCTCGCGCTTAAGACGACGCTTCTGATTGAAGAACTCCACGCTCTTACTGAAGAAGATTGccc < 1:2579543/69‑1 (MQ=255)
gTTGCTGCTCGCGCTTAAGACGACGCTTCTGATTGAAGAACTCCACGCTCTTACTGAAGAAGATTGccc < 1:520904/69‑1 (MQ=255)
gTTGCTGCTCGCGCTTAAGACGACGCTTCTGATTGAAGAACTCCACGCTCTTACTGAAGAAGATTGccc < 1:2504122/69‑1 (MQ=255)
gTTGCTGCTCGCGCTTAAGACGACGCTTCTGATTGAAGAACTCCACGCTCTTACTGAAGAAGATTGccc < 1:2372474/69‑1 (MQ=255)
ttGCTGCTCGCGCTTAAGACGACGCTTCTGATTGAAGAACTCCACGCTCTTACTGAAGAAGATTGccc < 1:2330944/68‑1 (MQ=255)
tgctgcTCGCGCTTAAGACGACGCTTCTGATTGAAGAACTCCACGCTCTTACTGAAGAAGATTGCCCAg < 1:3421818/69‑1 (MQ=255)
tgctgcTCGCGCTTAAGACGACGCTTCTGATTGAAGAACTCCACGCTCTTACTGAAGAAGATTGCCCAg < 1:2102210/69‑1 (MQ=255)
tgctgcTCGCGCTTAAGACGACGCTTCTGATTGAAGAACTCCACGCTCTTACTGAAGAAGATTGCCCAg < 1:3351657/69‑1 (MQ=255)
tgctgcTCGCGCTTAAGACGACGCTTCTGATTGAAGAACTCCACGCTCTTACTGAAGAAGATTGCCCAg < 1:3397026/69‑1 (MQ=255)
tgctgcTCGCGCTTAAGACGACGCTTCTGATTGAAGAACTCCACGCTCTTACTGAAGAAGATTGCCCAg < 1:1373434/69‑1 (MQ=255)
tgctgcTCGCGCTTAAGACGACGCTTCTGATTGAAGAACTCCACGCTCTTACTGAAGAAGATTGCCCAg < 1:1379231/69‑1 (MQ=255)
tgctgcTCGCGCTTAAGACGACGCTTCTGATTGAAGAACTCCACGCTCTTACTGAAGAAGATTGCCCAg < 1:2897311/69‑1 (MQ=255)
tgctgcTCGCGCTTAAGACGACGCTTCTGATTGAAGAACTCCACGCTCTTACTGAAGAAGATTGCCCAg < 1:1463468/69‑1 (MQ=255)
tgctgcTCGCGCTTAAGACGACGCTTCTGATTGAAGAACTCCACGCTCTTACTGAAGAAGATTGCCCAg < 1:1839333/69‑1 (MQ=255)
tgctgcTCGCGCTTAAGACGACGCTTCTGATTGAAGAACTCCACGCTCTTACTGAAGAAGATTGCCCAg < 1:619444/69‑1 (MQ=255)
tgctgcTCGCGCTTAAGACGACGCTTCTGATTGAAGAACTCCACGCTCTTACTGAAGAAGATTGCCCAg < 1:1964518/69‑1 (MQ=255)
tgctgcTCGCGCTTAAGACGACGCTTCTGATTGAAGAACTCCACGCTCTTACTGAAGAAGATTGCCCAg < 1:2106363/69‑1 (MQ=255)
tgctgcTCGCGCTTAAGACGACGCTTCTGATTGAAGAACTCCACGCTCTTAATGAAGAAGATTGCCCAg < 1:3177254/69‑1 (MQ=255)
gctgctCGCGCTTAAGGCGACGCTTCTGATTGAAGAACTCTACGCTCTTACTGAAGAAGATTGCCCAg > 1:663262/1‑68 (MQ=255)
gctgctCGCGCTTAAGGCGACGCTTCTGATTGAAGAACTCTACGCTCTTACTGAAGAAGATTGCCCAg > 1:41493/1‑68 (MQ=255)
gctgctCGCGCTTAAGGCGACGCTTCTGATTGAAGAACTCTACGCTCTTACTGAAGAAGATTGCCCAg > 1:1076748/1‑68 (MQ=255)
gctgctCGCGCTTAAGGCGACGCTTCTGATTGAAGAACTCTACGCTCTTACTGAAGAAGATTGCCCAg > 1:2897169/1‑68 (MQ=255)
gctgctCGCGCTTAAGACGACGCTTCTGATTGAAGAACTCCACGCTCTTACTGAAGAAGATTGCCCAg < 1:2206796/68‑1 (MQ=255)
ctgctCGCGCTTAAGACGACGCTTCTGATTGAAGAACTCCACGCTCTTACTGAAGAAGATTGccc < 1:398981/65‑1 (MQ=255)
ctCGCGCTTAAGGCGACGCTTCTGATTGAAGAACTCTACGCTCTTACTGaagaag < 1:2485577/55‑1 (MQ=255)
cgcgCTTAAGACGACGCTTCTGATTGAAGAACTCCACGCTCTTACTGAAGAAGATTGccc < 1:2334793/60‑1 (MQ=255)
cgcTTAAGACGACGCTTCTGATTGAAGAACTCCAGGCTCTTACTGAAGAAGATTGCCCAg < 1:1486925/60‑1 (MQ=255)
gcTTAAGGCGACGCTTCTGATTGAAGAACTCTACGCTCTTACTGAAGAAGATTGCCCAg < 1:3200928/59‑1 (MQ=255)
gcTTAAGGCGACGCTTCTGATTGAAGAACTCTACGCTCTTACTGAAGAAGATTGCCCAg < 1:2139532/59‑1 (MQ=255)
gcTTAAGGCGACGCTTCTGATTGAAGAACTCTACGCTCTTACTGAAGAAGATTGCCCAg < 1:2507309/59‑1 (MQ=255)
gcTTAAGGCGACGCTTCTGATTGAAGAACTCTACGCTCTTACTGAAGAAGATTGCCCAg < 1:2719296/59‑1 (MQ=255)
gcTTAAGGCGACGCTTCTGATTGAAGAACTCTACGCTCTTACTGAAGAAGATTGCCCAg < 1:2736329/59‑1 (MQ=255)
gcTTAAGGCGACGCTTCTGATTGAAGAACTCTACGCTCTTACTGAAGAAGATTGCCCAg < 1:3033336/59‑1 (MQ=255)
cTTAAGACGACGCTTCTGATTGAAGAACTCCACGCTCTTACTGAAGAAGATTGCCCAg < 1:2147980/58‑1 (MQ=255)
ttAAGGCGACGCTTCTGATTGAAGAACTCTACGCTCTTa < 1:2147349/39‑1 (MQ=255)
tAAGGCGACGCTTCTGATTGAAGAACTCTACGCTCTTACTGAAGAAGATTGCCCAGGTGACTACGGAg > 1:2340184/1‑68 (MQ=255)
tAAGGCGACGCTTCTGATTGAAGAACTCTACGCTCTTACTGAAGAAGATTGCCCAGGTGACTACGGAg > 1:2925206/1‑68 (MQ=255)
tAAGGCGACGCTTCTGATTGAAGAACTCTACGCTCTTACTGAAGAAGATTGCCCAGGTGACTACGGAg > 1:831126/1‑68 (MQ=255)
tAAGGCGACGCTTCTGATTGAAGAACTCTACGCTCTTACTGAAGAAGATTGCCCAGGTGACTACGGAg > 1:3125575/1‑68 (MQ=255)
tAAGGCGACGCTTCTGATTGAAGAACTCTACGCTCTTACTGAAGAAGATTGCCCAGGTGACTACGGAg > 1:1431247/1‑68 (MQ=255)
|
GATATCGTTGGCCTGGTTTAAGGAACGCGCTTCAGCCAGCAGTTGCTGCTCGCGCTTAAGGCGACGCTTCTGATTGAAGAACTCTACGCTCTTACTGAAGAAGATTGCCCAGGTGACTACGGAG > minE/2014523‑2014646
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
