Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,022,941 |
A→G |
38.3% |
C322C (TGT→TGC) |
ygiQ ← |
conserved hypothetical protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,022,941 | 0 | A | G | 38.3%
| 35.7
/ 47.4
| 73 | C322C (TGT→TGC) | ygiQ | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (10/35); new base G (9/19); total (19/54) |
| Fisher's exact test for biased strand distribution p-value = 4.15e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.34e-01 |
GGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCAC > minE/2022891‑2023009
|
gtgTTGATCCATACATAGCGGTCGCCATGTTTTTGCATCAATGCGCGGGCGCAGCCTGGGTTAGTTTCg < 1:2842892/67‑1 (MQ=255)
gggTTGATCCATACATAGCGGTCGCCATGTTTTTGCATCAATGCGCGGGCGCAGCCTGGGTTAGTTTCg < 1:657783/69‑1 (MQ=255)
gggTTGATCCATACATAGCGGTCGCCATGTTTTTGCATCAATGCGCGGGCGCAGCCTGGGTTAGTTTCg < 1:1812754/69‑1 (MQ=255)
gggTTGATCCATACATAGCGGTCGCCATGTTTTTGCATCAATGCGCGGGCGCAGCCTGGGTTAGTTTCg < 1:2840562/69‑1 (MQ=255)
gggTTGATCCATACATAGCGGTCGCCATGTTTTTGCATCAATGCGCGGGCGCAGCCTGGGTTAGTTTCg < 1:2816681/69‑1 (MQ=255)
gggTTGATCCATACATAGCGGTCGCCATGTTTTTGCATCAATGCGCGGGCGCAGCCTGGGTTAGTTTCg < 1:1969538/69‑1 (MQ=255)
gggTTGATCCATACATAGCGGTCGCCATGTTTTTGCATCAATGCGCGGGCGCAGCCTGGGTTAGTTTCg < 1:161787/69‑1 (MQ=255)
gggTTGATCCATACATAGCGGTCGCCATGTTTTTGCATCAATGCGCGGGCGCAGCCTGGGTTAGTTTCg < 1:337131/69‑1 (MQ=255)
gggTTGATCCATACATAGCGGTCGCCATGTTTTTGCATCAATGCGCGGGCGCAGCCTGGGTTAGTTTCg < 1:2687296/69‑1 (MQ=255)
gggTTGATCCATACATAGCGGTCGCCATGTTTTTGCATCAATGCGCGGGCGCAGCCTGGGTTAGTTTCg < 1:1591747/69‑1 (MQ=255)
gggTTGATCCATACATAGCGGTCGCCATGTTTTTGCATCAATGCGCGGGCGCAGCCTGGGTTAGTTTCg < 1:398853/69‑1 (MQ=255)
gggTTGATCCATACATAGCGGTCGCCATGTTTTTGCATCAATGCGCGGGCGCAGCCTGGGTTAGTTTCg < 1:661782/69‑1 (MQ=255)
gggTTGATCCATACATAGCGGTCGCCATGTTTTTGCATCAATGCGCGGGCGCAGCCTGGGTTAGTTTCg < 1:688120/69‑1 (MQ=255)
gggTTGATCCATACATAGCGGTCGCCATGTTTTTGCATCAATGCGCGGGCGCAGCCTGGGTTAGTTTCg < 1:1388037/69‑1 (MQ=255)
gggTTGATCCATACATAGCGGTCGCCATGTTTTTGCATCAATGCGCGGGCGCAGCCTGGGTTAGTTTCg < 1:769479/69‑1 (MQ=255)
gggTTGATCCATACATAGCGGTCGCCATGTTTTTGCATCAATGCGCGGGCGCAGCCTGGGTTAGTTTCg < 1:2363335/69‑1 (MQ=255)
gggTTGATCCATACATAGCGGTCGCCATGTTTTTGCATCAATGCGCGGGCGCAGCCTGGGTTAGTTTCg < 1:2086360/69‑1 (MQ=255)
gggTTGATCCATACATAGCGGTCGCCATGTTTTTGCATCAATGCGCGGGCGCAGCCTGGGTTAGTTTCg < 1:866687/69‑1 (MQ=255)
gggTTGATCCATACATAGCGGTCGCCATGTTTTTGCATCAATGCGCGGGCGCAGCCTGGGTTAGTTTCg < 1:20975/69‑1 (MQ=255)
gggTTGATCCACACATAGCTGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGggttggt < 1:1585849/65‑1 (MQ=255)
gggTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGggttggt < 1:3134171/65‑1 (MQ=255)
gggTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGggttggt < 1:2558357/65‑1 (MQ=255)
gggTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGggttggt < 1:2392297/65‑1 (MQ=255)
gggTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGggttggt < 1:2924466/65‑1 (MQ=255)
gggTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGggttggt < 1:1602754/65‑1 (MQ=255)
gggTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGggttggt < 1:1430781/65‑1 (MQ=255)
gggTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGggttggt < 1:820201/65‑1 (MQ=255)
ggTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGggttggt < 1:794522/64‑1 (MQ=255)
ccACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAg < 1:895340/69‑1 (MQ=255)
acaTAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATa < 1:1769760/69‑1 (MQ=255)
acaTAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATa < 1:1755383/69‑1 (MQ=255)
acaTAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATa < 1:2734385/69‑1 (MQ=255)
acaTAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATa < 1:1451155/69‑1 (MQ=255)
acaTAGCGGTCGCCATGTTTTTGCATCAATGCGCGGGCGCAGCCTGggtta > 1:286992/1‑50 (MQ=38)
acaTAGCGGTCGCCATGTTTTTGCATCAATGCGCGGGCGCAGCCTGggtta > 1:1086223/1‑50 (MQ=38)
acaTAGCGGTCGCCATGTTTTTGCATCAATGCGCGGGCGCAGCCTGggtta > 1:2257181/1‑50 (MQ=38)
acaTAGCGGTCGCCATGTTTTTGCATCAATGCGCGGGCGCAGCCTGggtta > 1:1379510/1‑50 (MQ=38)
acaTAGCGGTCGCCATGTTTTTGCATCAATGCGCGGGCGCAGCCTGggtta > 1:1466352/1‑50 (MQ=38)
acaTAGCGGTCGCCATGTTTTTGCATCAATGCGCGGGCGCAGCCTGggtta > 1:147416/1‑50 (MQ=38)
acaTAGCGGTCGCCATGTTTTTGCATCAATGCGCGGGCGCAGCCTGggtta > 1:1563738/1‑50 (MQ=38)
acaTAGCGGTCGCCATGTTTTTGCATCAATGCGCGGGCGCAGCCTGggtta > 1:1714260/1‑50 (MQ=38)
acaTAGCGGTCGCCATGTTTTTGCATCAATGCGCGGGCGCAGCCTGggtta > 1:1716454/1‑50 (MQ=38)
gCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAg > 1:2069125/1‑60 (MQ=255)
gCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAg > 1:960381/1‑60 (MQ=255)
gCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAg > 1:3299418/1‑60 (MQ=255)
gCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAg > 1:310085/1‑60 (MQ=255)
gCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAg > 1:1903925/1‑60 (MQ=255)
gCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAg > 1:2471142/1‑60 (MQ=255)
ggTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAg < 1:7740/69‑1 (MQ=255)
ggTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAg < 1:453399/69‑1 (MQ=255)
ggTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAg < 1:1613834/69‑1 (MQ=255)
ggTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAg < 1:3217155/69‑1 (MQ=255)
gTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGc < 1:1971212/69‑1 (MQ=255)
gTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGc < 1:3235737/69‑1 (MQ=255)
gTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGc < 1:3196709/69‑1 (MQ=255)
tCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGc < 1:2974537/68‑1 (MQ=255)
tCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGc < 1:1950271/68‑1 (MQ=255)
gCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTg > 1:3242510/1‑35 (MQ=255)
gCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATa > 1:75015/1‑58 (MQ=255)
gCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATg > 1:1159902/1‑69 (MQ=255)
gCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATg > 1:2361747/1‑69 (MQ=255)
tgtTTTTGCATCAATGCGCTGGCACAGCCTGGGTTGGTTTCGTTGTGCAGAATACGCGAAGCATGGGCg < 1:1972485/69‑1 (MQ=255)
tgtTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCATAATACGCGAAGCATGGGCg < 1:528051/69‑1 (MQ=255)
tgtTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCg < 1:2249340/69‑1 (MQ=255)
tgtTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCg < 1:2882664/69‑1 (MQ=255)
tgtTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCg < 1:1228851/69‑1 (MQ=255)
tgtTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCg < 1:759979/69‑1 (MQ=255)
tgtTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCg < 1:737919/69‑1 (MQ=255)
tttttGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTa < 1:3068084/69‑1 (MQ=255)
tttttGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTa < 1:635350/69‑1 (MQ=255)
tttttGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTa < 1:1574798/69‑1 (MQ=255)
tttttGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTa < 1:3278566/69‑1 (MQ=255)
tttttGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTa < 1:2613201/69‑1 (MQ=255)
acaGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGc < 1:579663/62‑1 (MQ=255)
acaGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGc < 1:604175/62‑1 (MQ=255)
acaGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGc < 1:2064350/62‑1 (MQ=255)
acaGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGc < 1:1834098/62‑1 (MQ=255)
acaGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGc < 1:1829965/62‑1 (MQ=255)
acaGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGc < 1:1322065/62‑1 (MQ=255)
acaGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGc < 1:259401/62‑1 (MQ=255)
acaGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGc < 1:1021764/62‑1 (MQ=255)
acaGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGc < 1:1701119/62‑1 (MQ=255)
acaGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCAc < 1:2820006/69‑1 (MQ=255)
acaGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCAc < 1:2419129/69‑1 (MQ=255)
acaGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCAc < 1:2012668/69‑1 (MQ=255)
acaGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCAc < 1:3233790/69‑1 (MQ=255)
|
GGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCAC > minE/2022891‑2023009
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
