Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,039,492 |
G→A |
60.5% |
R141R (CGC→CGT) |
ribB ← |
3,4 dihydroxy‑2‑butanone‑4‑phosphate synthase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,039,492 | 0 | G | A | 60.5%
| 39.0
/ 62.9
| 91 | R141R (CGC→CGT) | ribB | 3,4 dihydroxy‑2‑butanone‑4‑phosphate synthase |
| Reads supporting (aligned to +/- strand): ref base G (27/9); new base A (33/22); total (60/31) |
| Fisher's exact test for biased strand distribution p-value = 1.77e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.53e-01 |
GATCAATAGTTGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAATCGC > minE/2039437‑2039554
|
gATCGATAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGa > 1:3205240/1‑59 (MQ=255)
gATCGATAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGt > 1:512270/1‑62 (MQ=255)
gATCGATAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTg > 1:2357946/1‑63 (MQ=255)
gATCGATAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGaaaa > 1:470323/1‑69 (MQ=255)
gATCGATAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGaaaa > 1:2114579/1‑69 (MQ=255)
gATCGATAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGaaaa > 1:546245/1‑69 (MQ=255)
gATCGATAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGaaaa > 1:2482290/1‑69 (MQ=255)
gATCGATAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGaaaa > 1:522846/1‑69 (MQ=255)
gATCGATAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGaaaa > 1:3051977/1‑69 (MQ=255)
gATCGATAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGaaaa > 1:697736/1‑69 (MQ=255)
gATCGATAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGaaaa > 1:3123109/1‑69 (MQ=255)
gATCGATAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGaaaa > 1:3166001/1‑69 (MQ=255)
gATCGATAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGaaaa > 1:3217824/1‑69 (MQ=255)
gATCGATAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGaaaa > 1:1275433/1‑69 (MQ=255)
gATCGATAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGaaaa > 1:1259955/1‑69 (MQ=255)
gATCGATAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAACTGGGaaaa > 1:3054708/1‑69 (MQ=255)
gATCGAAAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGaaaa > 1:435884/1‑69 (MQ=255)
aTCGATAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGaaaa < 1:3327770/68‑1 (MQ=255)
aTCGATAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGaaaa < 1:501995/68‑1 (MQ=255)
aTCGATAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGaaaa < 1:3015684/68‑1 (MQ=255)
aTCGATAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGaaaa < 1:2628769/68‑1 (MQ=255)
aTCGATAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGaaaa < 1:44283/68‑1 (MQ=255)
aTCGATAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGaaaa < 1:466737/68‑1 (MQ=255)
aTCGATAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGaaaa < 1:2176274/68‑1 (MQ=255)
aTCGATAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGaaaa < 1:1084050/68‑1 (MQ=255)
aTCGATAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGaaaa < 1:2037603/68‑1 (MQ=255)
aTCGATAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGaaaa < 1:1112096/68‑1 (MQ=255)
cgaTAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGAAAACg > 1:284666/3‑68 (MQ=255)
cgaTAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGAAAACGt > 1:2214028/3‑69 (MQ=255)
cgaTAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGAAAACGt > 1:1686953/3‑69 (MQ=255)
cgaTAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGAAAACGt > 1:1073749/3‑69 (MQ=255)
cgaTAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGAAAACGt > 1:531306/3‑69 (MQ=255)
cgaTAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGAAAACGt > 1:1823740/3‑69 (MQ=255)
cgaTAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGAAAACGt > 1:1646717/3‑69 (MQ=255)
cgaTAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGAAAACGt > 1:2336842/3‑69 (MQ=255)
cgaTAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGAAAACGt > 1:2277825/3‑69 (MQ=255)
cgaTAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGAAAACGt > 1:224411/3‑69 (MQ=255)
cgaTAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGAAAACGt > 1:3361660/3‑69 (MQ=255)
cgaTAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGAAAACGt > 1:1469614/3‑69 (MQ=255)
cgaTAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGAAAACGt > 1:1882218/3‑69 (MQ=255)
cgaTAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGAAAACGt > 1:1326178/3‑69 (MQ=255)
cgaTAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGAAAACGt > 1:3230613/3‑69 (MQ=255)
cgaTAGTCGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGCGCACGAAGTGGGAAAACGt > 1:657071/3‑69 (MQ=255)
gTTGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGcc > 1:2836692/1‑68 (MQ=255)
cAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGATAACGTGGCCAGGACGa > 1:73569/1‑68 (MQ=255)
cAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGa > 1:2796655/1‑68 (MQ=255)
aTGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGTCGATTCAg < 1:3332930/69‑1 (MQ=255)
aTGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAg < 1:1946475/69‑1 (MQ=255)
aTGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAg < 1:2029147/69‑1 (MQ=255)
aTGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAg < 1:2128639/69‑1 (MQ=255)
aTGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAg < 1:1247840/69‑1 (MQ=255)
aTGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAg < 1:2296216/69‑1 (MQ=255)
aTGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAg < 1:2838746/69‑1 (MQ=255)
aTGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAg < 1:2212691/69‑1 (MQ=255)
aTGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAg < 1:1633184/69‑1 (MQ=255)
tGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACg > 1:2427689/1‑51 (MQ=255)
tGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACg > 1:1620142/1‑51 (MQ=255)
tGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACg > 1:695006/1‑51 (MQ=255)
tGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACg > 1:2398858/1‑51 (MQ=255)
tGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACg > 1:2423645/1‑51 (MQ=255)
tGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACg > 1:757953/1‑51 (MQ=255)
tGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGa > 1:2993775/1‑69 (MQ=255)
tGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGa > 1:1630962/1‑69 (MQ=255)
tGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGa > 1:3354820/1‑69 (MQ=255)
tGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGa > 1:1731172/1‑69 (MQ=255)
tGACCGCCACGCGTCAGTACACCACCTGCCTGAGAGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGa > 1:1927757/1‑69 (MQ=255)
aCCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGAt > 1:1867957/1‑68 (MQ=255)
ccaccTGCCTGAGCGCGAAGTGGGAAAACGTGGCTAGGACGATTCAGATCTGACGGTTTTGCGCCATc > 1:2948814/1‑68 (MQ=255)
ccaccTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATc > 1:3258596/1‑68 (MQ=255)
ccaccTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATc > 1:1364981/1‑68 (MQ=255)
ccaccTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATc > 1:1368858/1‑68 (MQ=255)
ccaccTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATc > 1:2337069/1‑68 (MQ=255)
ccaccTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATc > 1:1172480/1‑68 (MQ=255)
ccaccTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATc > 1:445393/1‑68 (MQ=255)
ccaccTGCCTGAGCGCGAAGTGGGAAAACGTGGCAAGGACGATTCAGATCTGACGGTTTTGCGCCATc > 1:1692217/1‑68 (MQ=255)
ctgCGCACGAAGTGGGAAAACGTGGCCCGTACGATTCAg < 1:575920/39‑1 (MQ=21)
ctgCGCACGAAGTGGGAAAACGTGGCCCGGACGATTCat < 1:85148/39‑2 (MQ=25)
ctgCGCACGAAGTGGGAAAACGTGGCCCGGACGATTCAg < 1:3383763/39‑1 (MQ=37)
ctgCGCACGAAGTGGGAAAACGTGGCCCGGACGATTCAg < 1:528875/39‑1 (MQ=37)
ctgCGCACGAAGTGGGAAAACGTGGCCCGGACGATTCAg < 1:2646450/39‑1 (MQ=37)
ctgCGCACGAAGTGGGAAAACGTGGCCCGGACGATTCAg < 1:2427070/39‑1 (MQ=37)
ctgCGCACGAAGTGGGAAAACGTGGCCCGGACGATTCAg < 1:1911636/39‑1 (MQ=37)
ctgCGCACGAAGTGGGAAAACGTGGCCCGGACGATTCAg < 1:1853939/39‑1 (MQ=37)
ctgCGCACGAAGTGGGAAAACGTGGCCCGGACGATTCAg < 1:1481927/39‑1 (MQ=37)
ctgCGCACGAAGTGGGAAAACGTGGCCCGGACGATTCAg < 1:1282311/39‑1 (MQ=37)
ctgCGCACGAAGTGGGAAAACGTGGCCCGGACGATTCAg < 1:1272388/39‑1 (MQ=37)
ctgCGCACGAAGTGGGAAAACGTGGCCCGGACGATTCAg < 1:1270260/39‑1 (MQ=37)
ctgAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAATCGc > 1:38024/1‑69 (MQ=255)
ctgAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAATCGc > 1:2694111/1‑69 (MQ=255)
ctgAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAATCGc > 1:2404874/1‑69 (MQ=255)
ctgAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAATCGc > 1:73455/1‑69 (MQ=255)
|
GATCAATAGTTGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAATCGC > minE/2039437‑2039554
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
