Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F21 I0 R1
|
476 |
35.3 |
2812105 |
96.7% |
2719305 |
61.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
2,039,900 |
A→G |
32.6% |
I130I (ATT→ATC) |
yedV ← |
predicted sensory kinase in two‑component regulatory system with YedW |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 2,039,900 | 0 | A | G | 32.6%
| 51.0
/ 41.2
| 46 | I130I (ATT→ATC) | yedV | predicted sensory kinase in two‑component regulatory system with YedW |
| Reads supporting (aligned to +/- strand): ref base A (13/18); new base G (5/10); total (18/28) |
| Fisher's exact test for biased strand distribution p-value = 7.49e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CATGTTATGTCTGGCTGAAGCCAATTTAGCCACAGTAACCGTTAATGATGGCGAAACTTCATCAATATTAATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTCTGTAAATGCCAGCTGCGCTGATTGTCTC > W3110S.gb/2039831‑2039968
|
cATGTTATGTCTGGCTGAAGCCAATTTAGCCACAGTAACCGTTAATGATGGCGAAACTTCATCAATATTaa > 1:473213/1‑71 (MQ=255)
gCTGAAGCCAATTTAGCCACAGTAACCGTTAATGATGGCGAAACTTCATCAATATTGATTCGTAAAGc < 1:842920/68‑1 (MQ=255)
gCTGAAGCCAATTTAGCCACAGTAACCGTTAATGATGGCGAAACTTCATCAATATTAATTCGTAAAGCatc > 1:2222836/1‑71 (MQ=255)
cAATTTAGCCACAGTAACCGTTAATGATGGCGAAACTTCATCAATATTAATTCGTAAAGCATCTATCTCTg < 1:1640090/71‑1 (MQ=255)
aaTTTAGCCACAGTAACCGTTAATGATGGCGAAACTTCATCAATATTaa > 1:2365456/1‑49 (MQ=255)
aaTTTAGCCACAGTAACCGTTAATGATGGCGAAACTTCATCAATATTaa > 1:2679805/1‑49 (MQ=255)
ccACAGTAACCGTTAATGATGGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCAtt < 1:2318367/70‑1 (MQ=255)
ccACAGTAACCGTTAATGATGGCGAAACTTCATCAATATTAATTCGTAAAGCa < 1:2764132/53‑1 (MQ=255)
ccACAGTAACCGTTAATGATGGCGAAACTTCATCAATATTAATTCGTAAAGCa < 1:1650613/53‑1 (MQ=255)
ccACAGTAACCGTTAATGATGGCGAAACTTCATCAATATTAATTCGTAAAGCATCTATCTCTGTATCATta < 1:584470/71‑1 (MQ=255)
cacaGTAACCGTTAATGATGGCGAAACTTCATCAATATTAATTCGTAAAGCATCTATCTCTGTATCATtaa < 1:2589781/71‑1 (MQ=255)
cacaGTAACCGTTAATGATGGCGAAACTTCATCAATATTAATTCGTAAAGCATCTATCTCTGTATCATtaa < 1:1509086/71‑1 (MQ=255)
cacaGTAACCGTTAATGATGGCGAAACTTCAT‑AATATTGATTCGTAAAGCat > 1:2648048/1‑52 (MQ=255)
acaGTAACCGTTAATGATGGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCATtaat < 1:1108038/71‑1 (MQ=255)
acaGTAACCGTTAATGATGGCGAAACTTCATCAATATTAATTCGTAAAGCATCTATCTCTGTATCATtaat < 1:575338/71‑1 (MQ=255)
acaGTAACCGTTAATGATGGCGAAACTTCATCAATATTAATTCGTAAAGCATCTATCTCTGTATCATtaat < 1:1357892/71‑1 (MQ=255)
caGTAACCGTTAATGATGGCGAAACTTCATCAATATTGATTCGTAAAGCatct < 1:36019/53‑1 (MQ=255)
caGTAACCGTTAATGATGGCGAAACTTCATCAATATTGATTCGTAAAGCatct < 1:1769537/53‑1 (MQ=255)
caGTAACCGTTAATGATGGCGAAACTTCATCAATATTAATTCGTAAAGCATCTATCTCTGTATCATtaata < 1:561366/71‑1 (MQ=255)
gTAACCGTTAATGATGGCGAAACTTCATCAATATTGATTCGTAAAGCatcta > 1:1557512/1‑52 (MQ=255)
gTAACCGTTAATGATGGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCATTaataat < 1:569378/71‑1 (MQ=255)
gTAACCGTTAATGATGGCGAAACTTCATCAATATTAATTCGTAAAGCATCTATCTCTGTATCATTaataat > 1:1014651/1‑71 (MQ=255)
aCCGTTAATGATGGCGAAACTTCATCAATATTAATTCGTAAAGCATCTATCTCTGTATCATtaataa > 1:2255377/1‑67 (MQ=255)
cGTTAATGATGGCGAAACTTCATCAATATTAATTCGTAAAGCatc < 1:194334/45‑1 (MQ=255)
ttAATGATGGCGAAACTTCATCAATATTAATTCGTAAAGCatcta > 1:316618/1‑45 (MQ=255)
aaTGATGGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTCTg > 1:352644/1‑69 (MQ=255)
atgatgGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTCTGTa < 1:2664332/70‑1 (MQ=255)
atgGCGAAACTTCATCAATATTAATTGGTAAAGCATCTATCTCTg > 1:26397/1‑45 (MQ=255)
atgGCGAAACTTCATCAATATTAATTCGTAAAGCATCTATCTCTg > 1:192371/1‑45 (MQ=255)
atgGCGAAACTTCATCAATATTAATTCGTAAAGCATCTATCTCTg > 1:2208892/1‑45 (MQ=255)
gCGAAACTTCATCAATATTGATTCGTAAAGCatct < 1:277656/35‑1 (MQ=255)
gCGAAACTTCATCAATATTAATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTCTGTAAATGcc < 1:2289171/70‑1 (MQ=255)
gCGAAACTTCATCAATATTAATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTCTGTAAATGc > 1:2175354/1‑69 (MQ=255)
aaCTTCATCAATATTAATTCGTAAAGCATCTATCTCTGTATCATtaat > 1:974390/1‑48 (MQ=255)
aaCTTCATCAATATTAATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTCTGTAAATGCCAGCTg > 1:760940/1‑71 (MQ=255)
aCTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTCTGTAAATGCCAGCTgc > 1:2513560/1‑71 (MQ=255)
aCTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTCTGTAAATGCCAGCTgc > 1:1961284/1‑71 (MQ=255)
aCTTCATCAATATTAATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTCTGTAAATGCCAGCTgc < 1:2546828/71‑1 (MQ=255)
aCTTCATCAATATTAATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTCTGTAAATGCCAGCTgc < 1:1668002/71‑1 (MQ=255)
cTTCATCAATATTAATTCGTAAAGCATCTATCTCTGTATCATtaataa < 1:1800454/48‑1 (MQ=255)
cTTCATCAATATTAATTCGTAAAGCATCTATCTCTGTATCATTaataat > 1:2674774/1‑49 (MQ=255)
cTTCATCAATATTAATTCGTAAAGCATCTATCTCTGTATCATTAATAATg > 1:1057683/1‑50 (MQ=255)
tcatcaATATTAATTCGTAAAGCATCTATCTCTGTa > 1:858251/1‑36 (MQ=255)
atcaATATTGATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTCTGTAAATGCCAGCTGCGCTg < 1:2164796/70‑1 (MQ=255)
atcaATATTAATTCGTAAAGCATCTATCTCTGTATCATtaat < 1:461383/42‑1 (MQ=255)
atcaATATTAATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTCTGTAAATGCCAGCTGCGCTg < 1:468695/70‑1 (MQ=255)
atcaATATTAATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTCTGTAAATGCCAGCTGCGCTg < 1:1214586/70‑1 (MQ=255)
tatTGATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTCTGTAAATGCCAGCTGCGCTGAt < 1:1645130/67‑1 (MQ=255)
ttAATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTCTGTAAATGCCAGCTGCGCTGATTGtctc < 1:2031214/71‑1 (MQ=255)
|
CATGTTATGTCTGGCTGAAGCCAATTTAGCCACAGTAACCGTTAATGATGGCGAAACTTCATCAATATTAATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTCTGTAAATGCCAGCTGCGCTGATTGTCTC > W3110S.gb/2039831‑2039968
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
