Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I1 R1
|
354 |
41.7 |
3110465 |
93.1% |
2895842 |
61.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
2,039,900 |
A→G |
I130I (ATT→ATC) |
yedV ← |
predicted sensory kinase in two‑component regulatory system with YedW |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 2,039,900 | 0 | A | G | 98.4%
| 223.1
/ ‑4.3
| 61 | I130I (ATT→ATC) | yedV | predicted sensory kinase in two‑component regulatory system with YedW |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (25/35); minor base C (0/1); total (25/36) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01 |
TGGCTGAAGCCAATTTAGCCACAGTAACCGTTAATGATGGCGAAACTTCATCAATATTAATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTCTGTAAATGCCAGCTGCGCTGA > W3110S.gb/2039842‑2039961
|
tGGCTGAAGCCAATTTAGCCACAGTAACCGTTAATGATGGCGAAACTTCATCAATATTGATTc > 1:846/1‑63 (MQ=255)
tGGCTGAAGCCAATTTAGCCACAGTAACCGTTAATGATGGCGAAACTTCATCAATATTGATTCg > 1:2175450/1‑64 (MQ=255)
tGGCTGAAGCCAATTTAGCCACAGTAACCGTTAATGATGGCGAAACTTCATCAATATTGATTCg > 1:991699/1‑64 (MQ=255)
tGGCTGAAGCCAATTTAGCCACAGTAACCGTTAATGATGGCGAAACTTCATCAATATTGATTCg > 1:9508/1‑64 (MQ=255)
tGGCTGAAGCCAATTTAGCCACAGTAACCGTTAATGATGGCGAAACTTCATCAATATTGATTCg > 1:812949/1‑64 (MQ=255)
tGGCTGAAGCCAATTTAGCCACAGTAACCGTTAATGATGGCGAAACTTCATCAATATTGATTCg > 1:323663/1‑64 (MQ=255)
tGGCTGAAGCCAATTTAGCCACAGTAACCGTTAATGATGGCGAAACTTCATCAATATTGATTCg > 1:2901733/1‑64 (MQ=255)
tGGCTGAAGCCAATTTAGCCACAGTAACCGTTAATGATGGCGAAACTTCATCAATATTGATTCg > 1:1040628/1‑64 (MQ=255)
tGGCTGAAGCCAATTTAGCCACAGTAACCGTTAATGATGGCGAAACTTCATCAATATTGATTCg > 1:1899259/1‑64 (MQ=255)
tGGCTGAAGCCAATTTAGCCACAGTAACCGTTAATGATGGCGAAACTTCATCAATATTGATTCg > 1:1799744/1‑64 (MQ=255)
tGGCTGAAGCCAATTTAGCCACAGTAACCGTTAATGATGGCGAAACTTCATCAATATTGATTCg > 1:1757686/1‑64 (MQ=255)
tGGCTGAAGCCAATTTAGCCACAGTAACCGTTAATGATGGCGAAACTTCATCAATATTGATTCg > 1:1683330/1‑64 (MQ=255)
tGGCTGAAGCCAATTTAGCCACAGTAACCGTTAATGATGGCGAAACTTCATCAATATTGATTCg > 1:1579196/1‑64 (MQ=255)
tGGCTGAAGCCAATTTAGCCACAGTAACCGTTAATGATGGCGAAACTTCATCAATATTGATTCg > 1:1575785/1‑64 (MQ=255)
tGGCTGAAGCCAATTTAGCCACAGTAACCGTTAATGATGGCGAAACTTCATCAATATTGATTCg > 1:1053707/1‑64 (MQ=255)
tGGCTGAAGCCAATTTAGCCACAGTAACCGTTAATGATGGCGAAACTTCATCAATATTGATTCg > 1:1091787/1‑64 (MQ=255)
tGGCTGAAGCCAATTTAGCCACAGTAACCGTTAATGATGGCGAAACTTCATCAATATTGATTCg > 1:1233697/1‑64 (MQ=255)
tGGCTGAAGCCAATTTAGCCACAGTAACCGTTAATGATGGCGAAACTTCATCAATATTGATTCg > 1:1410447/1‑64 (MQ=255)
tGGCTGAAGCCAATTTAGCCACAGTAACCGTTAATGATGGCGAAACTTCATCAATATTGATTCg > 1:1401449/1‑64 (MQ=255)
tGGCTGAAGCCAATTTAGCCACAGTAACCGTTAATGATGGCGAAACTTCATCAATATTGATTCg > 1:1131320/1‑64 (MQ=255)
tGGCTGAAGCCAATTTAGCCACAGTAACCGTTAATGATGGCGAAACTTCATCAATATTGATTCg > 1:1144027/1‑64 (MQ=255)
cacaGTACCCGTTAATGATGGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTAt > 1:87009/1‑65 (MQ=255)
cacaGTAACCGTTAATGATGGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTAt > 1:911548/1‑65 (MQ=255)
cacaGTAACCGTTAATGATGGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTAt > 1:1628078/1‑65 (MQ=255)
cacaGTAACCGTTAATGATGGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTAt > 1:588549/1‑65 (MQ=255)
gatgGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCGTTAATAATGCTTc < 1:1548927/64‑1 (MQ=255)
gatgGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTc < 1:78431/64‑1 (MQ=255)
gatgGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTc < 1:566754/64‑1 (MQ=255)
gatgGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTc < 1:562658/64‑1 (MQ=255)
gatgGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTc < 1:1165612/64‑1 (MQ=255)
gatgGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTc < 1:876721/64‑1 (MQ=255)
gatgGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTc < 1:2994567/64‑1 (MQ=255)
gatgGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTc < 1:2668273/64‑1 (MQ=255)
gatgGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTc < 1:2588179/64‑1 (MQ=255)
gatgGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTc < 1:2503514/64‑1 (MQ=255)
gatgGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTc < 1:2490074/64‑1 (MQ=255)
gatgGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTc < 1:2445902/64‑1 (MQ=255)
gatgGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTc < 1:1594590/64‑1 (MQ=255)
gatgGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTc < 1:129363/64‑1 (MQ=255)
gatgGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTc < 1:2009910/64‑1 (MQ=255)
gatgGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTc < 1:2026904/64‑1 (MQ=255)
gatgGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTc < 1:2094529/64‑1 (MQ=255)
gatgGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTc < 1:210591/64‑1 (MQ=255)
gatgGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTc < 1:2106382/64‑1 (MQ=255)
gatgGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTc < 1:212950/64‑1 (MQ=255)
gatgGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTc < 1:2199860/64‑1 (MQ=255)
gatgGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTc < 1:2259255/64‑1 (MQ=255)
gatgGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTc < 1:2287150/64‑1 (MQ=255)
gatgGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTc < 1:246064/64‑1 (MQ=255)
gatgGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGCATCATTAATAATGCTTc < 1:2959927/64‑1 (MQ=255)
gatgGCGAAACTTCATCAATATTCATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTc < 1:2994120/64‑1 (MQ=255)
atTGATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTCTGTAAATGCCAGCTGCGCTGa < 1:1186680/65‑1 (MQ=255)
atTGATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTCTGTAAATGCCAGCTGCGCTGa < 1:2752242/65‑1 (MQ=255)
atTGATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTCTGTAAATGCCAGCTGCGCTGa < 1:2690354/65‑1 (MQ=255)
atTGATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTCTGTAAATGCCAGCTGCGCTGa < 1:22142/65‑1 (MQ=255)
atTGATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTCTGTAAATGCCAGCTGCGCTGa < 1:844341/65‑1 (MQ=255)
atTGATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTCTGTAAATGCCAGCTGCGCTGa < 1:1275457/65‑1 (MQ=255)
atTGATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTCTGTAAATGCCAGCTGCGCTGa < 1:1645727/65‑1 (MQ=255)
atTGATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTCTGTAAATGCCAGCTGCGCTGa < 1:1414276/65‑1 (MQ=255)
atTGATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTCTGTAAATGCCAGCTGCGCTGa < 1:982735/65‑1 (MQ=255)
atTGATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTCTGTAAATGCCAGCTGCGCTGa < 1:1522449/65‑1 (MQ=255)
|
TGGCTGAAGCCAATTTAGCCACAGTAACCGTTAATGATGGCGAAACTTCATCAATATTAATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTCTGTAAATGCCAGCTGCGCTGA > W3110S.gb/2039842‑2039961
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
