Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,080,941 |
A→G |
50.6% |
A212A (GCA→GCG) |
exuT → |
hexuronate transporter |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,080,941 | 0 | A | G | 50.6%
| ‑1.5
/ 84.6
| 93 | A212A (GCA→GCG) | exuT | hexuronate transporter |
| Reads supporting (aligned to +/- strand): ref base A (38/8); new base G (39/8); total (77/16) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01 |
GCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCTATAAACATCCGCGCGACCAGAAGCATCTG > minE/2080881‑2081007
|
gcTGGTGGTATGGGCAATCGTGATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCGTTGAGCtt > 1:1051113/1‑69 (MQ=255)
gcTGGTGGTATGGGCAATCGTGATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCGTTGAGCtt > 1:3176098/1‑69 (MQ=255)
gcTGGTGGTATGGGCAATCGTGATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCGTTGAGCtt > 1:2846525/1‑69 (MQ=255)
gcTGGTGGTATGGGCAATCGTGATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCGTTGAGCtt > 1:3207102/1‑69 (MQ=255)
gcTGGTGGTATGGGCAATCGTGATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCGTTGAGCtt > 1:2837666/1‑69 (MQ=255)
gcTGGTGGTATGGGCAATCGTGATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCGTTGAGCtt > 1:1995766/1‑69 (MQ=255)
gcTGGTGGTATGGGCAATCGTGATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCGTTGAGCtt > 1:328353/1‑69 (MQ=255)
gcTGGTGGTATGGGCAATCGTGATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCGTTGAGCtt > 1:282854/1‑69 (MQ=255)
gcTGGTGGTATGGGCAATCGTGATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCGTTGAGCtt > 1:166267/1‑69 (MQ=255)
gcTGGTGGTATGGGCAATCGTGATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCGTTGAGCtt > 1:162148/1‑69 (MQ=255)
gcTGGTGGTATGGGCAATCGTGATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCGTTGAGCtt > 1:348555/1‑69 (MQ=255)
gcTGGTGGTATGGGCAATCGTGATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCGTTGAGCtt > 1:1546807/1‑69 (MQ=255)
gcTGGTGGTATGGGCAATCGTGATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCGTTGAGCtt > 1:1518754/1‑69 (MQ=255)
gcTGGTGGTATGGGCAATCGTGATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCGTTGAGCtt > 1:1449880/1‑69 (MQ=255)
gcTGGTGGTATGGGCAATCGTGATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCGTTGAGCtt > 1:5266/1‑69 (MQ=255)
gcTGGTGGTATGGGCAATCGTGATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCGTTGAGCtt > 1:1210730/1‑69 (MQ=255)
gcTGGTGGTATGGGCAATCGTGATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCGTTGAGCtt > 1:1268970/1‑69 (MQ=255)
gcTGGTGGTATGGGCAATCGTGATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCGTTGAGCtt > 1:2520287/1‑69 (MQ=255)
gcTGGTGGTATGGGCAATCGTGATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCGTTGAGCtt > 1:2792876/1‑69 (MQ=255)
gcTGGTGGTATGGGCAATCGTGATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCGTTGAGCtt > 1:2742136/1‑69 (MQ=255)
gcTGGTGGTATGGGCAATCGTGATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCGTTGAGCtt > 1:680434/1‑69 (MQ=255)
ggtggtATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTAt > 1:2870623/1‑69 (MQ=255)
ggtggtATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTAt > 1:2484880/1‑69 (MQ=255)
ggtggtATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTAt > 1:1708862/1‑69 (MQ=255)
ggtggtATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTAt > 1:1624467/1‑69 (MQ=255)
ggtggtATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTAt > 1:1441466/1‑69 (MQ=255)
ggtggtATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTAt > 1:508276/1‑69 (MQ=255)
ggtggtATGCGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTAt > 1:645586/1‑69 (MQ=255)
ggCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAg > 1:733316/1‑54 (MQ=255)
ggCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTAt > 1:329110/1‑60 (MQ=255)
ggCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGat > 1:2306010/1‑69 (MQ=255)
ggCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGat > 1:2664561/1‑69 (MQ=255)
ggCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGat > 1:2143152/1‑69 (MQ=255)
ggCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGat > 1:2029536/1‑69 (MQ=255)
ggCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGat > 1:1287096/1‑69 (MQ=255)
cGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGatggcatg > 1:3164235/1‑69 (MQ=255)
cGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGatggcatg > 1:2189441/1‑69 (MQ=255)
cGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGatggcatg > 1:236367/1‑69 (MQ=255)
cGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGatggcatg > 1:1879581/1‑69 (MQ=255)
cGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGatggcatg > 1:646260/1‑69 (MQ=255)
cGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGatggcatg > 1:797075/1‑69 (MQ=255)
cGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGatggcatg > 1:2729861/1‑69 (MQ=255)
cGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGatggcat > 1:2048337/1‑68 (MQ=255)
cGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGatggcat > 1:3363947/1‑68 (MQ=255)
cGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGatggca > 1:2044476/1‑67 (MQ=255)
aTGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGATGGCATGGCTg < 1:2861867/69‑1 (MQ=255)
aTGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGATGGCATGGCTg < 1:936660/69‑1 (MQ=255)
aTGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGATGGCATGGCTg < 1:2554946/69‑1 (MQ=255)
aTGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGATGGCATGGCTg < 1:2567277/69‑1 (MQ=255)
aTGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGATGGCATGGCTg < 1:2448803/69‑1 (MQ=255)
aTGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGATGGCATGGCTg < 1:1603326/69‑1 (MQ=255)
cacaGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGATGGCATGGCTGAtt > 1:1285169/1‑69 (MQ=255)
cacaGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGATGGCATGGCTGAtt > 1:2038891/1‑69 (MQ=255)
cacaGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGATGGCATGGCTGAtt > 1:1501401/1‑69 (MQ=255)
cacaGCTGGCAGATGGCATTTATCATATCCGGTGCATTGAGCTTTATCTGGGCGATGGCATGGCTGAt > 1:703349/1‑68 (MQ=255)
ggCAGATGGCATTTATCATCTCCGGTGCGTTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCtata < 1:2150963/69‑1 (MQ=255)
ggCAGATGGCATTTATCATCTCCGGTGCGTTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCtata < 1:2314349/69‑1 (MQ=255)
ggCAGATGGCATTTATCATCTCCGGTGCGTTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCtata < 1:3283621/69‑1 (MQ=255)
ggCAGATGGCATTTATCATCTCCGGTGCGTTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCtata < 1:3306559/69‑1 (MQ=255)
ggCAGATGGCATTTATCATCTCCGGTGCGTTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCtata < 1:599852/69‑1 (MQ=255)
ggCAGATGGCATTTATCATCTCCGGTGCGTTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCtata < 1:716588/69‑1 (MQ=255)
ggCAGATGGCATTTATCATCTCCGGTGCGTTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCtata < 1:2642115/69‑1 (MQ=255)
ggCAGATGGCATTTATCATCTCCGGGGCGTTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCtata < 1:408553/69‑1 (MQ=255)
gCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCTATaaa > 1:492429/1‑63 (MQ=255)
gCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCTATAAACATcc > 1:1059280/1‑68 (MQ=255)
gCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCTATAAACATCcg > 1:2137267/1‑69 (MQ=255)
gCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCTATAAACATCcg > 1:2339628/1‑69 (MQ=255)
gCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCTATAAACATCcg > 1:1303971/1‑69 (MQ=255)
gCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCTATAAACATCcg > 1:2352720/1‑69 (MQ=255)
gCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCTATAAACATCcg > 1:1428735/1‑69 (MQ=255)
gCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCTATAAACATCcg > 1:2514745/1‑69 (MQ=255)
ttATCATCTCCGGTGCGTTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCtata > 1:2932613/1‑57 (MQ=255)
ttATCATCTCCGGTGCGTTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCtata > 1:2587972/1‑57 (MQ=255)
ttATCATCTCCGGTGCGTTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCtata > 1:828451/1‑57 (MQ=255)
ttATCATCTCCGGTGCGTTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCtata > 1:2603762/1‑57 (MQ=255)
ttATCATCTCCGGTGCGTTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCtata > 1:2724762/1‑57 (MQ=255)
ttATCATCTCCGGTGCGTTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCtata > 1:1314313/1‑57 (MQ=255)
ttATCATCTCCGGTGCGTTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCtata > 1:1429971/1‑57 (MQ=255)
ttATCATCTCCGGTGCGTTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCtata > 1:519012/1‑57 (MQ=255)
ttATCATCTCCGGTGCGTTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCtata > 1:1559244/1‑57 (MQ=255)
ttATCATCTCCGGTGCGTTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCtata > 1:2494867/1‑57 (MQ=255)
ttATCATCTCCGGTGCGTTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCtata > 1:453137/1‑57 (MQ=255)
ttATCATCTCCGGTGCGTTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCtata > 1:2830264/1‑57 (MQ=255)
ttATCATCTCCGGTGCGTTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCtata > 1:1830628/1‑57 (MQ=255)
ttATCATCTCCGGTGCGTTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCtata > 1:2845202/1‑57 (MQ=255)
ttATCATCTCCGGTGCGTTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCtata > 1:186476/1‑57 (MQ=255)
ttATCATCTCCGGTGCGTTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCtata > 1:1968673/1‑57 (MQ=255)
ttATCATCTCCGGTGCGTTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCtata > 1:2285767/1‑57 (MQ=255)
ttATCATCTCCGGTGCGTTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCtata > 1:3057777/1‑57 (MQ=255)
catcTCCGGTGCATTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCTATAAACATCCGCGCGa > 1:847150/1‑66 (MQ=255)
gTGCATTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCTATAAACATCCGCGCGACCAGAAGc < 1:516311/66‑1 (MQ=255)
gTGCATTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCTATAAACATCCGCGCGACCAGAAGc < 1:2685730/66‑1 (MQ=255)
gCATTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCTATAAACATCCGCGCGACCAGAAGCATCTg > 1:2526834/1‑69 (MQ=255)
|
GCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCTATAAACATCCGCGCGACCAGAAGCATCTG > minE/2080881‑2081007
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
