Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,130,401 |
A→G |
41.5% |
F168F (TTT→TTC) |
mtr ← |
tryptophan transporter of high affinity |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,130,401 | 0 | A | G | 41.5%
| 20.8
/ 46.8
| 65 | F168F (TTT→TTC) | mtr | tryptophan transporter of high affinity |
| Reads supporting (aligned to +/- strand): ref base A (18/20); new base G (13/14); total (31/34) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.05e-01 |
TAAGACGCATTGCTTTCGGCGACGTTGAACAATGTCGCAGGCTGCACATGCCCCAGCAGGCTACCAAAGGTGAGGAAGAAGGTAATGACTTTCGCCCCCAGCACAATCGCTGTCATGCGACTGACGGCTTT > minE/2130336‑2130466
|
tAAGACTCATTGCTTTCGGCGACGTTGAACAATGTCGCAGGCTGCACATGCCCCAGCAGGCTACCAAAg < 1:1033654/69‑1 (MQ=255)
tAAGACGCATTGCTTTCGGCGACGTTGAACAATGTCGCAGGCTGGACATGCCCCAGCAGGCTACCaaa < 1:93526/68‑1 (MQ=255)
tAAGACGCATTGCTTTCGGCGACGTTGAACAATGTCGCAGGCTGCACATGCCCCAGCAGGCTACCaaa < 1:698360/68‑1 (MQ=255)
tAAGACGCATTGCTTTCGGCGACGTTGAACAATGTCGCAGGCTGCACATGCCCCAGCAGGCTACCaaa < 1:2136373/68‑1 (MQ=255)
tAAGACGCATTGCTTTCGGCGACGTTGAACAATGTCGCAGGCTGCACATGCCCCAGCAGGCTACCaaa < 1:714634/68‑1 (MQ=255)
tAAGACGCATTGCTTTCGGCGACGTTGAACAATGTCGCAGGCTGCACATGCCCCAGCAGGCTACCaaa < 1:2335987/68‑1 (MQ=255)
tAAGACGCATTGCTTTCGGCGACGTTGAACAATGTCGCAGGCTGCACATGCCCCAGCAGGCTACCaaa < 1:1885379/68‑1 (MQ=255)
tAAGACGCATTGCTTTCGGCGACGTTGAACAATGTCGCAGGCTGCACATGCCCCAGCAGGCTACCaaa < 1:1793678/68‑1 (MQ=255)
tAAGACGCATTGCTTTCGGCGACGTTGAACAATGTCGCAGGCTGCACATGCCCCAGCAGGCTACCaaa < 1:1639404/68‑1 (MQ=255)
tAAGACGCATTGCTTTCGGCGACGTTGAACAATGTCGCAGGCTGCACATGCCCCAGCAGGCTACCaaa < 1:1632927/68‑1 (MQ=255)
tAAGACGCATTGCTTTCGGCGACGTTGAACAATGTCGCAGGCTGCACATGCCCCAGCAGGCTACCaaa < 1:1626609/68‑1 (MQ=255)
tAAGACGCATTGCTTTCGGCGACGTTGAACAATGTCGCAGGCTGCACATGCCCCAGCAGGCTACCaaa < 1:2572912/68‑1 (MQ=255)
tAAGACGCATTGCTTTCGGCGACGTTGAACAATGTCGCAGGCTGCACATGCCCCAGCAGGCTACCaaa < 1:493918/68‑1 (MQ=255)
tAAGACGCATTGCTTTCGGCGACGTTGAACAATGTCGCAGGCTGCACATGCCCCAGCAGGCTACCaaa < 1:1548390/68‑1 (MQ=255)
tAAGACGCATTGCTTTCGGCGACGTTGAACAATGTCGCAGGCTGCACATGCCCCAGCAGGCTACCAAAg < 1:2653923/69‑1 (MQ=255)
tAAGACGCATTGCTTTCGGCGACGTTGAACAATGTCGCAGGCTGCACATGCCCCAGCAGGCTACCAAAg < 1:2022004/69‑1 (MQ=255)
tAAGACGCATTGCTTTCGGCGACGTTGAACAATGTCGCAGGCTGCACATGCCCCAGCAGGCTACCAAAg < 1:2021253/69‑1 (MQ=255)
tAAGACGCATTGCTTTCGGCGACGTTGAACAATGTCGCAGGCTGCACATGCCCCAGCAGGCTACCAAAg < 1:1744405/69‑1 (MQ=255)
tAAGACGCATTGCTTTCGGCGACGTTGAACAATGTCGCAGGCTGCACATGCCCCAGCAGGCTACCAAAg < 1:726449/69‑1 (MQ=255)
tAAGACGCATTGCTTTCGGCGACGTTGAACAATGTCGCAGGCTGCACATGCCCCAGCAGGCTACCAAAg < 1:887075/69‑1 (MQ=255)
tAAGACGCATTACTTTCGGCGACGTTGAACAATGTCGCAGGCTGCACATGCCCCAGCAGGCTACCaaa < 1:2684097/68‑1 (MQ=255)
aCGCATTGCTTTCGGCGACGTTGAACAATGTCGCAGGCTGCACATGCCCCAGCAGGCTACCAAAGGTg < 1:2399777/68‑1 (MQ=255)
aCGCATTGCTTTCGGCGACGTTGAACAATGTCGCAGGCTGCACATGCCCCAGCAGGCTACCAAAGGTg < 1:468432/68‑1 (MQ=255)
aCGCATTGCTTTCGGCGACGTTGAACAATGTCGCAGGCTGCACATGCCCCAGCAGGCTACCAAAGGTg < 1:2887775/68‑1 (MQ=255)
aCGCATTGCTTTCGGCGACGTTGAACAATGTCGCAGGCTGCACATGCCCCAGCAGGCTACCAAAGGTg < 1:131530/68‑1 (MQ=255)
aCGCATTGCTTTCGGCGACGTTGAACAATGTCGCAGGCTGCACATGCCCCAGCAGGCTACCAAAGGTg < 1:294238/68‑1 (MQ=255)
aCGCATTGCTTTCGGCGACGTTGAACAATGTCGCAGGCTGCACATGCCCCAGCAGGCTACCAAAGGTg < 1:2534272/68‑1 (MQ=255)
aCGCATTGCTTTCGGCGACGTTGAACAATGTCGCAGGCTGCACATGCCCCAGCAGGCTACCAAAGGTg < 1:3040532/68‑1 (MQ=255)
gCTTTCGGCGACGTTGAACAATGTCGCAGGCTGCACATGCCCCAGCAGGCTACCAAAGGTGAGgaagaa > 1:339937/1‑69 (MQ=255)
gCTTTCGGCGACGTTGAACAATGTCGCAGGCTGCACATGCCCCAGCAGGCTACCAAAGGTGAGgaagaa > 1:863348/1‑69 (MQ=255)
tgCGACGTTGTTCAACGTCGCAGGCTGCACATGCCCCAGCAGGCTACCGAAGGTGAGGAAGAAGGTAAt > 1:3594/2‑69 (MQ=255)
tgCGACATTGTTCAACGTCGCAGGCTGCACATGCCCCGGCAGGCTACCGAAGGTGAGGAAGAAGGTAAt > 1:1379762/2‑69 (MQ=25)
tgCGACATTGTTCAACGTCGCAGGCTGCACATGCCCCAGCAGGCTACCGAAGGTGAGGAAGAAGGTaa > 1:2536464/2‑68 (MQ=37)
tgCGACATTGTTCAACGTCGCAGGCTGCACATGCCCCAGCAGGCTACCGAAGGTGAGGAAGAAGGTaa > 1:2290954/2‑68 (MQ=37)
tgCGACATTGTTCAACGTCGCAGGCTGCACATGCCCCAGCAGGCTACCGAAGGTGAGGAAGAAGGTAAt > 1:2658812/2‑69 (MQ=37)
tgCGACATTGTTCAACGTCGCAGGCTGCACATGCCCCAGCAGGCTACCGAAGGTGAGGAAGAAGGTAAt > 1:2283440/2‑69 (MQ=37)
tgCGACATTGTTCAACGTCGCAGGCTGCACATGCCCCAGCAGGCTACCGAAGGTGAGGAAGAAGGTAAt > 1:456002/2‑69 (MQ=37)
tgCGACATTGTTCAACGTCGCAGGCTGCACATGCCCCAGCAGGCTACCGAAGGTGAGGAAGAAGGTAAt > 1:1917685/2‑69 (MQ=37)
tgCGACATTGTTCAACGTCGCAGGCTGCACATGCCCCAGCAGGCTACCGAAGGTGAGGAAGAAGGTAAt > 1:759783/2‑69 (MQ=37)
tgCGACATTGTTCAACGTCGCAGGCTGCACATGCCCCAGCAGGCTACCGAAGGTGAGGAAGAAGGTAAt > 1:811204/2‑69 (MQ=37)
tgCGACATTGTTCAACGTCGCAGGCTGCACATGCCCCAGCAGGCTACCGAAGGTGAGGAAGAAGGTAAt > 1:415445/2‑69 (MQ=37)
tgCGACATTGTTCAACGTCGCAGGCTGCACATGCCCCAGCAGGCTACCGAAGGTGAGGAAGAAGGTAAt > 1:1526326/2‑69 (MQ=37)
tgCGACATTGTTCAACGTCGCAGGCTGCACATGCCCCAGCAGGCTACCGAAGGTGAGGAAGAAGGTAAt > 1:1089204/2‑69 (MQ=37)
ggCGACGTTGAACAATGTCGCAGGCTGCACATGCCCCAGCAGGCTACCGAAGGTGAGGAAGAAGGTaa < 1:2968174/68‑1 (MQ=255)
ggCGACGTTGAACAATGTCGCAGGCTGCACATGCCCCAGCAGGCTACCGAAGGTGAGGAAGAAGGTaa < 1:37043/68‑1 (MQ=255)
ggCGACGTTGAACAATGTCGCAGGCTGCACATGCCCCAGCAGGCTACCGAAGGTGAGGAAGAAGGTaa < 1:2036500/68‑1 (MQ=255)
ggCGACGTTGAACAATGTCGCAGGCTGCACATGCCCCAGCAGGCTACCGAAGGTGAGGAAGAAGGTaa < 1:599399/68‑1 (MQ=255)
ggCGACGTTGAACAATGTCGCAGGCTGCACATGCCCCAGCAGGCTACCGAAGGTGAGGAAGAAGGTaa < 1:2223543/68‑1 (MQ=255)
ggCGACGTTGAACAATGTCGCAGGCTGCACATGCCCCAGCAGGCTACCGAAGGTGAGGAAGAAGGTaa < 1:2071513/68‑1 (MQ=255)
ggCGACGTTGAACAATGTCGCAGGCTGCACATGCCCCAGCAGGCTACCGAAGGTGAGGAAGAAGGTaa < 1:1905484/68‑1 (MQ=255)
ggCGACGTTGAACAATGTCGCAGGCTGCACATGCCCCAGCAGGCTACCGAAGGTGAGGAAGAAGGTaa < 1:189644/68‑1 (MQ=255)
ggCGACGTTGAACAATGTCGCAGGCTGCACATGCCCCAGCAGGCTACCGAAGGTGAGGAAGAAGGTaa < 1:85796/68‑1 (MQ=255)
ggCGACGTTGAACAATGTCGCAGGCTGCACATGCCCCAGCAGGCTACCGAAGGTGAGGAAGAAGGTaa < 1:1433594/68‑1 (MQ=255)
ggCGACGTTGAACAATGTCGCAGGCTGCACATGCCCCAGCAGGCTACCGAAGGTGAGGAAGAAGGTaa < 1:1389877/68‑1 (MQ=255)
gCGACGTTGAACAATGTCGCAGGCTGCACATGCCCCAGCAGGCTACCGAAGGTGAGGAAGAAGGTaa < 1:3429362/67‑1 (MQ=255)
gCGACGTTGAACAATGTCGCAGGCTGCACATGCCCCAGCAGGCTACCGAAGGCGAGGAAGAAGGTaa < 1:1563873/67‑1 (MQ=255)
attGTCGCAGGCT‑CACATGCCCCAGCAGGCTACCAAAGGTg < 1:3176815/39‑1 (MQ=38)
gTCGCAGGCTGCACATGCCCCAGCAGGCTACCGAAGGTGAGGAAGAAGGTaa < 1:1116691/52‑1 (MQ=255)
gTCGCAGGCTGCACATGCCCCAGCAGGCTACCAAAGGTGAGgaaga > 1:954800/1‑46 (MQ=255)
gTCGCAGGCTGCACATGCCCCAGCAGGCTACCAAAGGTGAGgaaga > 1:3230599/1‑46 (MQ=255)
gTCGCAGGCTGCACATGCCCCAGCAGGCTACCAAAGGTGAGgaaga > 1:1443994/1‑46 (MQ=255)
gTCGCAGGCTGCACATGCCCCAGCAGGCTACCAAAGGTGAGgaaga > 1:162899/1‑46 (MQ=255)
gTCGCAGGCTGCACATGCCCCAGCAGGCTACCAAAGGTGAGgaag > 1:3381801/1‑45 (MQ=255)
tGCCCCAGCAGGCTACCAAAGGTGAGGAAGAAGGTAATGACTTTCGCCCCCAGCACAATCGCTGTCAt > 1:1052299/1‑68 (MQ=255)
tGCCCCAGCAGGCTACCAAAGGTGAGGAAGAAGGTAATGACTTTCGCCCCCAGCACAATCGCTGTCAt > 1:1551943/1‑68 (MQ=255)
tGCCCCAGCAGGCTACCAAAGGTGAGGAAGAAGGTAATGACTTTCGCCCCCAGCACAATCGCTGTCAt > 1:549448/1‑68 (MQ=255)
tGCCCCAGCAGGCTACCAAAGGTGAGGAAGAAGGTAATGACTTTCGCCCCCAGCACAATCGCTGTCAt > 1:264828/1‑68 (MQ=255)
tGCCCCAGCAGGCTACCAAAGGTGAGGAAGAAGGTAATGACTTTCGCCCCCAGCACAATCGCTGTCAt > 1:2738631/1‑68 (MQ=255)
tGCCCCAGCAGGCTACCAAAGGTGAGGAAGAAGGTAATGACTTTCGCCCCCAGCACAATCGCTGTCAt > 1:3229501/1‑68 (MQ=255)
ccccAGCAGGCTACCAAAGGTGAGGAAGAAGGTAATGACTTTCGCCCCCAGCACAATCGCTGTCATGCg > 1:1689992/1‑69 (MQ=255)
ccccAGCAGGCTACCAAAGGTGAGGAAGAAGGTAATGACTTTCGCCCCCAGCACAATCGCTGTCATGCg > 1:2878853/1‑69 (MQ=255)
ccccAGCAGGCTACCAAAGGTGAGGAAGAAGGTAATGACTTTCGCCCCCAGCACAATCGCTGTCATGCg > 1:871748/1‑69 (MQ=255)
ccccAGCAGGCTACCAAAGGTGAGGAAGAAGGTAATGACTTTCGCCCCCAGCACAATCGCTGTCATGCg > 1:1359046/1‑69 (MQ=255)
ccccAGCAGGCTACCAAAGGTGAGGAAGAAGGTAATGACTTTCGCCCCCAGCACAATCGCTGTCATGCg > 1:948228/1‑69 (MQ=255)
aCCAAAGGTGAGGAAGAAGGTAATGACTTTCGCCCCCAGCACAATCGCTGTCATGCGACTGACGGCttt < 1:2573487/69‑1 (MQ=255)
aCCAAAGGTGAGGAAGAAGGTAATGACTTTCGCCCCCAGCACAATCGCTGTCATGCGACTGACGGCttt < 1:2264185/69‑1 (MQ=255)
aCCAAAGGTGAGGAAGAAGGTAATGACTTTCGCCCCCAGCACAATCGCTGTCATGCGACTGACGGCttt < 1:2072916/69‑1 (MQ=255)
aCCAAAGGTGAGGAAGAAGGTAATGACTTTCGCCCCCAGCACAATCGCTGTCATGCGACTGACGGCttt < 1:1981538/69‑1 (MQ=255)
aCCAAAGGTGAGGAAGAAGGTAATGACTTTCGCCCCCAGCACAATCGCTGTCATGCGACTGACGGCttt < 1:1223719/69‑1 (MQ=255)
|
TAAGACGCATTGCTTTCGGCGACGTTGAACAATGTCGCAGGCTGCACATGCCCCAGCAGGCTACCAAAGGTGAGGAAGAAGGTAATGACTTTCGCCCCCAGCACAATCGCTGTCATGCGACTGACGGCTTT > minE/2130336‑2130466
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
