Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F1 I0 R1	4577	57.4	3436498	77.7%	2670158	63.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,145,954	A→G	54.2%	V251V (GTT→GTC)	yhbX ←	predicted hydrolase, inner membrane

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,145,954	0	A	G	54.2%	18.1 / 78.3	96	V251V (GTT→GTC)	yhbX	predicted hydrolase, inner membrane
Reads supporting (aligned to +/- strand): ref base A (20/24); new base G (8/44); total (28/68)
Fisher's exact test for biased strand distribution p-value = 1.59e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGTGTAAGGTGCGCCGCTTATTGCTTGATTAAACAGTTTGATCTGTTTTCTTTGTGCTTCAACTTGCGGTGTCGTAGAGCGTGTATATCCATACAAAGACATATTGTCGACACGTACAGACTC  >  minE/2145893‑2146016

cAGTGTAATGTGCACCGCTTATTGCTTGATTAAACAGTTTGATCTGTTTTCTTTGTGCTTCGACTTGCg                                                         <  1:2976548/69‑1 (MQ=255)

cAGTGTAAGGTGCACCGCTTATTGCTTGATTAAACAGTTTGATCTGTTTTCTTTGTGCTTCGACTTGct                                                         <  1:1587004/69‑2 (MQ=255)

cAGTGTAAGGTGCACCGCTTATTGCTTGATTAAACAGTTTGATCTGTTTTCTTTGTGCTTCGACTTGCg                                                         <  1:3049351/69‑1 (MQ=255)

cAGTGTAAGGTGCACCGCTTATTGCTTGATTAAACAGTTTGATCTGTTTTCTTTGTGCTTCGACTTGCg                                                         <  1:1837775/69‑1 (MQ=255)

cAGTGTAAGGTGCACCGCTTATTGCTTGATTAAACAGTTTGATCTGTTTTCTTTGTGCTTCGACTTGCg                                                         <  1:3309836/69‑1 (MQ=255)

cAGTGTAAGGTGCACCGCTTATTGCTTGATTAAACAGTTTGATCTGTTTTCTTTGTGCTTCGACTTGCg                                                         <  1:2022305/69‑1 (MQ=255)

cAGTGTAAGGTGCACCGCTTATTGCTTGATTAAACAGTTTGATCTGTTTTCTTTGTGCTTCGACTTGCg                                                         <  1:1684361/69‑1 (MQ=255)

cAGTGTAAGGTGCACCGCTTATTGCTTGATTAAACAGTTTGATCTGTTTTCTTTGTGCTTCGACTTGCg                                                         <  1:1461639/69‑1 (MQ=255)

cAGTGTAAGGTGCACCGCTTATTGCTTGATTAAACAGTTTGATCTGTTTTCTTTGTGCTTCGACTTGCg                                                         <  1:1026903/69‑1 (MQ=255)

cAGTGTAAGGTGCACCGCTTATTGCTTGATTAAACAGTTTGATCTGTTTTCTTTGTGCTTCGACTTGCg                                                         <  1:3007011/69‑1 (MQ=255)

cAGTGTAAGGTGCACCGCTTATTGCTTGATTAAACAGTTTGATCTGTTTTCTTTGTGCTTCGACTTGCg                                                         <  1:28673/69‑1 (MQ=255)

cAGTGTAAGGTGCACCGCTTATTGCTTGATTAAACAGTTTGATCTGTTTTCTTTGTGCTTCGACTTGCg                                                         <  1:86487/69‑1 (MQ=255)

cAGTGTAAGGTGCACCGCTTATTGCTTGATTAAACAGTTTGATCTGTTTTCTTTGTGCTTCGACTTGCg                                                         <  1:3006695/69‑1 (MQ=255)

cAGTGTAAGGTGCACCGCTTATTGCTTGATTAAACAGTTTGATCTGTTTTCTTTGTGCTTCGACTTGCg                                                         <  1:2647262/69‑1 (MQ=255)

cAGTGTAAGGTGCACCGCTTATTGCTTGATTAAACAGTTTGATCTGTTTTCTTTGTGCTTCGACTTGCg                                                         <  1:1139225/69‑1 (MQ=255)

cAGTGTAAGGTGCACCGCTTATTGCTTGATTAAACAGTTTGATCTGTTTTCTTTGTGCTTCGACTTGCg                                                         <  1:267816/69‑1 (MQ=255)

 aGTGTAAGGTGCACCGCTTATTGCTTGATTAAACAGTTTGATCTGTTTTCTTTGTGCTTCGACTTGCg                                                         <  1:2896117/68‑1 (MQ=255)

  gtgtAAGGTGCACCGCTTATTGCTTGATTAAACAGTTTGATCTGTTTTCTTTGTGCTTCGACTTGCg                                                         <  1:193356/67‑1 (MQ=255)

  gtgtAAGGTGCACCGCTTATTGCTTGATTAAACAGTTTGATCTGTTTTCTTTGTGCTTCGACTTGCGgt                                                       <  1:2053493/69‑1 (MQ=255)

  gtgtAAGGTGCACCGCTTATTGCTTGATTAAACAGTTTGATCTGTTTTCTTTGTGCTTCGACTTGCGgt                                                       <  1:2982303/69‑1 (MQ=255)

  gtgtAAGGTGCACCGCTTATTGCTTGATTAAACAGTTTGATCTGTTTTCTTTGTGCTTCGACTTGCGgt                                                       <  1:313257/69‑1 (MQ=255)

  gtgtAAGGTGCACCGCTTATTGCTTGATTAAACAGTTTGATCTGTTTTCTTTGTGCTTCGACTTGCGgt                                                       <  1:3118456/69‑1 (MQ=255)

  gtgtAAGGTGCACCGCTTATTGCTTGATTAAACAGTTTGATCTGTTTTCTTTGTGCTTCGACTTGCGgt                                                       <  1:2311783/69‑1 (MQ=255)

  gtgtAAGGTGCACCGCTTATTGCTTGATTAAACAGTTTGATCTGTTTTCTTTGTGCTTCGACTTGCGgt                                                       <  1:211154/69‑1 (MQ=255)

  gtgtAAGGTGCACCGCTTATTGCTTGATTAAACAGTTTGATCTGTTTTCTTTGTGCTTCGACTTGCGgt                                                       <  1:2113746/69‑1 (MQ=255)

  gtgtAAGGTGCACCGCTTATTGCTTGATTAAACAGTTTGATCTGTTTTCTTTGTGCTTCGACTTGCGgt                                                       <  1:2412891/69‑1 (MQ=255)

  gtgtAAGGTGCACCGCTTATTGCTTGATTAAACAGTTTGATCTGTTTTCTTTGTGCTTCGACTTGCGgt                                                       <  1:2179443/69‑1 (MQ=255)

  gtgtAAGGTGCACCGCTTATTGCTTGATTAAACAGTTTGATCTGTTTTCTTTGTGCTTCGACTTGCGgt                                                       <  1:223645/69‑1 (MQ=255)

  gtgtAAGGTGCACCGCTTATTGCTTGATTAAACAGTTTGATCTGTTTTCTTTGTGCTTCGACTTGCGgt                                                       <  1:1826100/69‑1 (MQ=255)

  gtgtAAGGTGCACCGCTTATTGCTTGATTAAACAGTTTGATCTGTTTTCTTTGTGCTTCGACTTGCGgt                                                       <  1:1060123/69‑1 (MQ=255)

  gtgtAAGGTGCACCGCTTATTGCTTGATTAAACAGTTTGATCTGTTTTCTTTGTGCTTCGACTTGCGgt                                                       <  1:1204642/69‑1 (MQ=255)

  gtgtAAGGTGCACCGCTTATTGCTTGATTAAACAGTTTGATCTGTTTTCTTTGTGCTTCGACTTGCGgt                                                       <  1:743821/69‑1 (MQ=255)

  gtgtAAGGTGCACCGCTTATTGCTTGATTAAACAGTTTGATCTGTTTTCTTTGTGCTTCGACTTGCGgt                                                       <  1:2961777/69‑1 (MQ=255)

  gtgtAAGGTGCACCGCTTATTGCTTGATTAAACAGTTTGATCTGTTTTCTTTGTGCTTCGACTTGCGgt                                                       <  1:705486/69‑1 (MQ=255)

  gtgtAAGGTGCACCGCTTATTGCTTGATTAAACAGTTTGATCTGTTTTCTTTGTGCTTCGACTTGCGgt                                                       <  1:1317514/69‑1 (MQ=255)

  gtgtAAGGTGCACCGCTTATTGCTTGATTAAACAGTTTGATCTGTTTTCTTTGTGCTTCGACTTGCGgt                                                       <  1:64423/69‑1 (MQ=255)

  gtgtAAGGTGCACCGCTTATTGCTTGATTAAACAGTTTGATCTGTTTTCTTTGTGCTTCGACTTGCGgt                                                       <  1:1424968/69‑1 (MQ=255)

  gtgtAAGGTGCACCGCTTATTGCTTGATTAAACAGTTTGATCTGTTTTCTTTGTGCTTCGACTTGCGgt                                                       <  1:2613232/69‑1 (MQ=255)

  gtgtAAGGTGCACCGCTTATTGCTTGATTAAACAGTTTGATCTGTTTTCTTTGTGCTTCGACTTGCGgt                                                       <  1:373131/69‑1 (MQ=255)

  gtgtAAGGTGCACCGCTTATTGCTTGATTAAACAGTTTGATCTGTTTTCTTTGTGCTTCGACTTGCGgt                                                       <  1:2549161/69‑1 (MQ=255)

  gtgtAAGGTGCACCGCTTATTGCTTGATTAAACAGTTTGATATGTTTTCTTTGTGCTTCGACTTGCGgt                                                       <  1:338068/69‑1 (MQ=255)

   tgtAAGGTGCACCGCTTATTGCTTGATTAAACAGTTTGATCTGTTTTCTTTGTGCTTCGACTTGCGgt                                                       <  1:2374955/68‑1 (MQ=255)

     tAAGGTGCGCCGCTTATTGCTTGATTAAACAGTTTGATCTGTTTTCTTTGTGCTTCAACTTGCGgtgt                                                     >  1:59757/1‑68 (MQ=255)

     tAAGGTGCGCCGCTTATTGCTTGATTAAACAGTTTGATCTGTTTTCTTTGTGCTTCAACTTGCGgtgt                                                     >  1:1592473/1‑68 (MQ=255)

          tGCGCCGCTTATTGCTTGATTAAACAGTTTGATCTGTTTTCTTTGTGCTTCAACTTGCGGTGTCGTaga                                               >  1:1835706/1‑69 (MQ=255)

          tGCGCCGCTTATTGCTTGATTAAACAGTTTGATCTGTTTTCTTTGTGCTTCAACTTGCGGTGTCGTa                                                 <  1:3026872/67‑1 (MQ=255)

          tGCGCCGCTTATTGCTTGATTAAACAGTTTGATCTGTTTTCTTTGTGCTTCAACTCGCGGTGTCGTa                                                 <  1:2819617/67‑1 (MQ=255)

               cgcTTATTGCTTGATTAAACAGTTTGATCTGTTTTCTTTGTGCTTCAACTTGCGGTGTCGTAGAGCgtg                                          <  1:2565823/69‑1 (MQ=255)

               cgcTTATTGCTTGATTAAACAGTTTGATCTGTTTTCTTTGTGCTTCAACTTGCGGTGTCGTAGAGCgtg                                          <  1:1168310/69‑1 (MQ=255)

               cgcTTATTGCTTGATTAAACAGTTTGATCTGTTTTCTTTGTGCTTCAACTTGCGGTGTCGTAGAGCgtg                                          <  1:995641/69‑1 (MQ=255)

                   tATTGCTTGATTAAACAGTTTGATCTGTTTTCTTTGTGCTTCAACTTGCGGTGTCg                                                   >  1:2221101/1‑56 (MQ=255)

                   tATTGCTTGATTAAACAGTTTGATCTGTTTTCTTTGTGCTTCAACTTGCGGTGTCg                                                   >  1:499926/1‑56 (MQ=255)

                   tATTGCTTGATTAAACAGTTTGATCTGTTTTCTTTGTGCTTCAACTTGCGGTGTCg                                                   >  1:934879/1‑56 (MQ=255)

                    aTTGCTTGATTAAACAGTTTGATCTGTTTTCTTTGTGCTTCGACTTGCg                                                         <  1:2790234/49‑1 (MQ=255)

                     ttGCTTGATTAAACAGTTTGATCTGTTTTCTTTGTGCTTCGACTTGCGgt                                                       <  1:1060182/50‑1 (MQ=255)

                      tGCTTGATTAAACAGTTTGATCTGTTTTCTTTGTGCTTCAACTTGCGGTGTCGTGGAGCGTGTATATc                                    <  1:469387/68‑1 (MQ=255)

                      tGCTTGATTAAACAGTTTGATCTGTTTTCTTTGTGCTTCAACTTGCGGTGTCGTGGAGCGTGTATATc                                    <  1:1259691/68‑1 (MQ=255)

                      tGCTTGATTAAACAGTTTGATCTGTTTTCTTTGTGCTTCAACTTGCGGTGTCGTAGAGCGTGTATATc                                    <  1:2409555/68‑1 (MQ=255)

                      tGCTTGATTAAACAGTTTGATCTGTTTTCTTTGTGCTTCAACTTGCGGTGTCGTAGAGCGTGTATATc                                    <  1:346306/68‑1 (MQ=255)

                      tGCTTGATTAAACAGTTTGATCTGTTTTCTTTGTGCTTCAACTTGCGGTGTCGTAGAGCGTGTATATc                                    <  1:2753999/68‑1 (MQ=255)

                       gCTTTATTAAACAGTTTGATCTGTTTTCTTTGTGCTTAAACTTGAGGTGTCGTATAGCGTGTATATGCa                                  >  1:2507074/1‑69 (MQ=255)

                       gCTTGATTAAACAGTTTGATCTGTTTTCTTTGTGCTTCAACTTGCGGTGTCGTAGAGCGTGTATATc                                    <  1:1452341/67‑1 (MQ=255)

                       gCTTGATTAAACAGTTTGATCTGTTTTCTTTGTGCTTCAACTTGCGGTGTCGTAGAGCGTGTATATCCa                                  >  1:1475288/1‑69 (MQ=255)

                       gCTTGATTAAACAGTTTGATCTGTTTTCTTTGTGCTTCAACTTGCGGTGTCGTAGAGCGTGTATATCCa                                  >  1:93778/1‑69 (MQ=255)

                       gCTTGATTAAACAGTTTGATCTGTTTTCTTTGTGCTTCAACTTGCGGTGTCGTAGAGCGTGTATATCCa                                  >  1:1857986/1‑69 (MQ=255)

                       gCTTGATTAAACAGTTTGATCTGTTTTCTTTGTGCTTCAACTTGCGGTGTCGTAGAGCGTGTATATCCa                                  >  1:2558621/1‑69 (MQ=255)

                       gCTTGATTAAACAGTTTGATCTGTTTTCTTTGTGCTTCAACTTGCGGTGTCGTAGAGCGTGTATATCCa                                  >  1:2502996/1‑69 (MQ=255)

                       gCTTGATTAAACAGTTTGATCTGTTTTCTTTGTGCTTCAACTTGCGGTGTCGTAGAGCGTGTATATCCa                                  >  1:737206/1‑69 (MQ=255)

                       gCTTGATTAAACAGTTTGATCTGTTTTCTTTGTGCTTCAACTTGCGGTGTCGTAGAGCGTGTATATCCa                                  >  1:2300698/1‑69 (MQ=255)

                       gCTTGATTAAACAGTTTGATCTGTTTTCTTTGTGCTTCAACTTGCGGTGTCGTAGAGCGTGTATATCCa                                  >  1:2230553/1‑69 (MQ=255)

                       gCTTGATTAAACAGTTTGATCTGTTTTCTTTGTGCTTCAACTTGCGGTGTCGTAGAGCGTGTATATCCa                                  >  1:1991406/1‑69 (MQ=255)

                       gCTTGATTAAACAGTTTGATCTGTTTTCTTTGTGCTTCAACTTGCGGTGTCGTAGAGCGTGTATATCCa                                  >  1:1417040/1‑69 (MQ=255)

                       gCTTGAATAAACAGTTTGATCTGTTTTCTTTGTGCTTCAACTTGCGGTGTCGTAGAGCGTGTATATCCa                                  >  1:1168/1‑69 (MQ=255)

                               aaaCAGTTTGATCTGTTTTCTTTGTGCTTCAACTTGCGGTGTCGTAGAGCGTGTATATCCATACAAAGa                          <  1:1502565/69‑1 (MQ=255)

                               aaaCAGTTTGATCTGTTTTCTTTGTGCTTCAACTTGCGGTGTCGTAGAGCGTGTATATCCATACAAAGa                          <  1:306938/69‑1 (MQ=255)

                               aaaCAGTTTGATCTGTTTTCTTTGTGCTTCAACTTGCGGTGTCGTAGAGCGTGTATATCCATACAAAGa                          <  1:2744631/69‑1 (MQ=255)

                                aaCAGTTTGATCTGTTTTCTTTGTGCTTCAACTTGCGGTGTCGTAGAGCGTGTATATCCATACAAAGa                          <  1:1209091/68‑1 (MQ=255)

                                   aGTTTGATCTGTTTTCTTTGTGCTTCAACTTGCGGTGTCGTAGAGCGTGTATATCCATACAAAGACata                      <  1:1296353/69‑1 (MQ=255)

                                   aGTTTGATCTGTTTTCTTTGTGCTTCAACTTGCGGTGTCGTAGAGCGTGTATATCCATACAAAGACata                      <  1:1048288/69‑1 (MQ=255)

                                   aGTTTGATCTGTTTTCTTTGTGCTTCAACTTGCGGTGTCGTAGAGCGTGTATATCCATACAAAGACata                      <  1:1693945/69‑1 (MQ=255)

                                   aGTTTGATCTGTTTTCTTTGTGCTTCAACTTGCGGTGTCGTAGAGCGTGTATATCCATACAAAGACata                      <  1:2117636/69‑1 (MQ=255)

                                   aGTTTGATCTGTATTCTTTTTGCTTCAACTTGCGGAGTCGTAGAGCGTGTATATCCATACAAAGTCata                      <  1:332754/69‑1 (MQ=255)

                                     tttGATCTGTTTTCTTTGTGCTTCAACTTGCGGTGTCGTAGAGCGTGTATATCCATACAAAGACATAtt                    <  1:2846421/69‑1 (MQ=255)

                                      ttGATCTGTTTTCTTTGTGCTTCAACTTGCGGTGTCGTAGAGCgtg                                          >  1:2840878/1‑46 (MQ=255)

                                       tGATCTGTTTTCTTTGTGCTTCAACTTGCGGTGTCGTAGAGCGTGTATATCCATACAAAGACATATTg                   >  1:94431/1‑68 (MQ=255)

                                                     tgtgCTTCGACTTGCGGTGTCGTAGAGCGTGTATATCCATACAAAGCCATATTGTCGACACGTACAGAc    >  1:2769286/1‑69 (MQ=255)

                                                     tgtgCTTCGACTTGCGGTGTCGTAGAGCGTGTATATCCATACAAAGACATATTGTCGACACGTACAGAc    >  1:3382912/1‑69 (MQ=255)

                                                     tgtgCTTCGACTTGCGGTGTCGTAGAGCGTGTATATCCATACAAAGACATATTGTCGACACGTACAGAc    >  1:1476584/1‑69 (MQ=255)

                                                     tgtgCTTCGACTTGCGGTGTCGTAGAGCGTGTATATCCATACAAAGACATATTGTCGACACGTACAGAc    >  1:2050047/1‑69 (MQ=255)

                                                     tgtgCTTCGACTTGCGGTGTCGTAGAGCGTGTATATCCATACAAAGACATATTGTCGACACGTACAGAc    >  1:2385537/1‑69 (MQ=255)

                                                     tgtgCTTCGACTTGCGGTGTCGTAGAGCGTGTATATCCATACAAAGACATATTGTCGACACGTACAGAc    >  1:843548/1‑69 (MQ=255)

                                                     tgtgCTTCGACTTGCGGTGTCGTAGAGCGTGTATATCCATACAAAGACATATTGTCGACACGTACAGAc    >  1:2622134/1‑69 (MQ=255)

                                                     tgtgCTTCGACTTGCGGTGTCGTAGAGCGTGTATATCCATACAAAGACATATTGTCGACACGTACAGAc    >  1:929886/1‑69 (MQ=255)

                                                        gCTTCAACTTGCGGTGTCGTAGAGCGTGTATATCCATACAAAGACATATTGTCGACACGTACAGACTc  <  1:1688838/68‑1 (MQ=255)

                                                        gCTTCAACTTGCGGTGTCGTAGAGCGTGTATATCCATACAAAGACATATTGTCGACACGTACAGACTc  <  1:2791542/68‑1 (MQ=255)

                                                        gCTTCAACTTGCGGTGTCGTAGAGCGTGTATATCCATACAAAGACATATTGTCGACACGTACAGACTc  <  1:1231469/68‑1 (MQ=255)

CAGTGTAAGGTGCGCCGCTTATTGCTTGATTAAACAGTTTGATCTGTTTTCTTTGTGCTTCAACTTGCGGTGTCGTAGAGCGTGTATATCCATACAAAGACATATTGTCGACACGTACAGACTC  >  minE/2145893‑2146016

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A