Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,210,179 |
A→C |
29.6% |
T454T (ACT→ACG) |
yhdP ← |
conserved membrane protein, predicted transporter |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,210,179 | 0 | A | C | 29.6%
| 104.3
/ 66.0
| 98 | T454T (ACT→ACG) | yhdP | conserved membrane protein, predicted transporter |
| Reads supporting (aligned to +/- strand): ref base A (17/52); new base C (17/12); total (34/64) |
| Fisher's exact test for biased strand distribution p-value = 2.21e-03 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01 |
TTGGCTTTAACGTCAATATTACGCCCATCCAGCTGGAAACCTTTGTTATTGTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGGCGCACGGAATACCGTTTCGTAAGGCATCTTTG > minE/2210114‑2210240
|
ttggctttAACGTCAATATTACGCCCATCCAGCTGGAAACCTTTGTTATTGTTCAGCCAGCTTATAGtt > 1:1146457/1‑69 (MQ=255)
ttAACGTCAATATTACGCCCATCCAGCTGGAAACCTTTGTCATTGTTCAGCCAGCTTATCGTTGCCTgg > 1:286350/1‑69 (MQ=255)
ttAACGTCAATATTACGCCCATCCAGCTGGAAACCTTTGTCATTGTTCAGCCAGCTTATCGTTGCCTgg > 1:2185524/1‑69 (MQ=255)
ttAACGTCAATATTACGCCCATCCAGCTGGAAACCTTTGTCATTGTTCAGCCAGCTTATCGTTGCCTgg > 1:221106/1‑69 (MQ=255)
ttAACGTCAATATTACGCCCATCCAGCTGGAAACCTTTGTCATTGTTCAGCCAGCTTATCGTTGCCTgg > 1:2225753/1‑69 (MQ=255)
ttAACGTCAATATTACGCCCATCCAGCTGGAAACCTTTGTCATTGTTCAGCCAGCTTATCGTTGCCTgg > 1:1534108/1‑69 (MQ=255)
ttAACGTCAATATTACGCCCATCCAGCTGGAAACCTTTGTCATTGTTCAGCCAGCTTATCGTTGCCTgg > 1:2311982/1‑69 (MQ=255)
ttAACGTCAATATTACGCCCATCCAGCTGGAAACCTTTGTCATTGTTCAGCCAGCTTATCGTTGCCTgg > 1:2602716/1‑69 (MQ=255)
ttAACGTCAATATTACGCCCATCCAGCTGGAAACCTTTGTCATTGTTCAGCCAGCTTATCGTTGCCTgg > 1:948622/1‑69 (MQ=255)
ttAACGTCAATATTACGCCCATCCAGCTGGAAACCTTTGTCATTGTTCAGCCAGCTTATCGTTGCCTgg > 1:2673968/1‑69 (MQ=255)
ttAACGTCAATATTACGCCCATCCAGCTGGAAACCTTTGTCATTGTTCAGCCAGCTTATCGTTGCCTgg > 1:2921376/1‑69 (MQ=255)
ttAACGTCAATATTACGCCCATCCAGCTGGAAACCTTTGTCATTGTTCAGCCAGCTTATCGTTGCCTgg > 1:128381/1‑69 (MQ=255)
ttAACGTCAATATTACGCCCATCCAGCTGGAAACCTTTGTCATTGTTCAGCCAGCTTATCGTTGCCTgg > 1:3242212/1‑69 (MQ=255)
ttAACGTCAATATTACGCCCATCCAGCTGGAAACCTTTGTCATTGTTCAGCCAGCTTATCGTTGCCTgg > 1:3328134/1‑69 (MQ=255)
ttAACGTCAATATTACGCCCATCCAGCTGGAAACCTTTGTCATTGTTCAGCCAGCTTATCGTTGCCTgg > 1:419175/1‑69 (MQ=255)
ttAACGTCAATATTACGCCCATCCAGCTGGAAACCTTTGTCATTGTTCAGCCAGCTTATCGTTGCCTgg > 1:466640/1‑69 (MQ=255)
ttAACGTCAATATTACGCCCATCCAGCTGGAAACCTTTGTCATTGTTCAGCCAGCTTATCGTTGCCTgg > 1:547395/1‑69 (MQ=255)
ttAACGTCAATATTACGCCCATCCAGCTGGAAACCTTTGTCATTGTTCAGCCAGCTTATCGTTGCCTg > 1:789975/1‑68 (MQ=255)
gTCAATATTACGCCCATCCAGCTGGAAACCTTTGTTATTGTTCAGCCAGCTTATAGTTGCCTGGCCGt > 1:1739727/1‑68 (MQ=255)
gTCAATATTACGCCCATCCAGCTGGAAACCTTTGTTATTGTTCAGCCAGCTTATAGTTGCCTGGCCGTc > 1:2810146/1‑69 (MQ=255)
ccAGCTGGAAACCTTTGTTATTGTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGgcg < 1:2318712/69‑1 (MQ=255)
ccAGCTGGAAACCTTTGTTATTGTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGgcg < 1:2823238/69‑1 (MQ=255)
ccAGCTGGAAACCTTTGTTATTGTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGgcg < 1:1278967/69‑1 (MQ=255)
ccAGCTGGAAACCTTTGTTATTGTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGgcg < 1:483914/69‑1 (MQ=255)
ccAGCTGGAAACCTTTGTTATTGTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGgcg < 1:3019891/69‑1 (MQ=255)
ccAGCTGGAAACCTTTGTTATTGTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGgcg < 1:3023963/69‑1 (MQ=255)
ccAGCTGGAAACCTTTGTTATTGTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGgcg < 1:3196593/69‑1 (MQ=255)
ccAGCTGGAAACCTTTGTTATTGTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGgcg < 1:1862030/69‑1 (MQ=255)
cAGCTGGAAACCTTTGTTATTGTTCGGCCAGCTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGgcgc > 1:2371171/1‑69 (MQ=255)
cAGCTGGAAACCTTTGTTATTGTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGgcgc > 1:51627/1‑69 (MQ=255)
cAGCTGGAAACCTTTGTTATTGTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGgcgc > 1:2063979/1‑69 (MQ=255)
cAGCTGGAAACCTTTGTTATTGTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGgcgc > 1:2983256/1‑69 (MQ=255)
cAGCTGGAAACCTTTGTTATTGTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGgcgc > 1:3223960/1‑69 (MQ=255)
cAGCTGGAAACCTTTGTTATTGTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGgcgc > 1:955685/1‑69 (MQ=255)
cAGCTGGAAACCTTTGTTATTGTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGgcgc > 1:756263/1‑69 (MQ=255)
cAGCTGGAAACCTTTGTTATTGTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGgcg > 1:2524827/1‑68 (MQ=255)
cAGCTGGAAACCTTTGTTATTGTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGgcg > 1:1474400/1‑68 (MQ=255)
tgtaaaCCTTTGTTATTGTTCATCCAGCTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGGCGCACgg < 1:1256439/66‑1 (MQ=255)
tGGAAACCTTTGTTATTGTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGGCGCACgg < 1:233546/69‑1 (MQ=255)
tGGAAACCTTTGTTATTGTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGGCGCACgg < 1:518240/69‑1 (MQ=255)
tGGAAACCTTTGTTATTGTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGGCGCACgg < 1:3083236/69‑1 (MQ=255)
tGGAAACCTTTGTTATTGTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGGCGCACgg < 1:3077790/69‑1 (MQ=255)
tGGAAACCTTTGTTATTGTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGGCGCACgg < 1:2001769/69‑1 (MQ=255)
tGGAAACCTTTGTTATTGTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGGCGCACgg < 1:698585/69‑1 (MQ=255)
tGGAAACCTTTGTTATTGTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGGCGCACgg < 1:1585351/69‑1 (MQ=255)
tGGAAACCTTTGTTATTGTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGGCGCACgg < 1:1384828/69‑1 (MQ=255)
tGGAAACCTTTGTTATTGTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGGCGCACgg < 1:2890540/69‑1 (MQ=255)
tGGAAACCTTTGTTATTGTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGGCGCACgg < 1:2868600/69‑1 (MQ=255)
tGGAAACCTTTGTTATTGTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGGCGCACgg < 1:3242234/69‑1 (MQ=255)
tGGAAACCTTTGTTATTGTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGGCGCACgg < 1:583971/69‑1 (MQ=255)
tGGAAACCTTTGTTATTGTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGGCGCACgg < 1:2697496/69‑1 (MQ=255)
tGGAAACCTTTGTTATTGTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGGCGCACgg < 1:2319218/69‑1 (MQ=255)
tGGAAACCTTTGTTATTGTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGGCGCACgg < 1:1234955/69‑1 (MQ=255)
tGGAAACCTTTGTTATTGTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGGCGCACgg < 1:3331700/69‑1 (MQ=255)
tGGAAACCTTTGTTATTGTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGGCGCACgg < 1:2643743/69‑1 (MQ=255)
tGGAAACCTTTGTTATTGTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGGCGCACgg < 1:2498209/69‑1 (MQ=255)
tGGAAACCTTTGTTATTGTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGGCGCACgg < 1:589373/69‑1 (MQ=255)
tGGAAACCTTTGTTATTGTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGGCGCACgg < 1:2580609/69‑1 (MQ=255)
ttGTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGATTTGTa > 1:1446326/1‑43 (MQ=255)
ttGTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGATTTCTa > 1:2141374/1‑43 (MQ=255)
ttGTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGATTTCTa > 1:1964678/1‑43 (MQ=255)
ttGTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGATTTCTa > 1:324693/1‑43 (MQ=255)
ttGTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGATTTCTa > 1:3149643/1‑43 (MQ=255)
ttGTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGGCGCACgg < 1:1581525/54‑1 (MQ=255)
tGTTCAGCCAGCTTATCGTTGCCTGGCCGTCGGCGATTTCCAGTGGCGCACGGAATACCGTTTCGTAAg < 1:1010402/69‑1 (MQ=255)
tGTTCAGCCAGCTTATCGTTGCCTGGCCGTCGGCGATTTCCAGTGGCGCACGGAATACCGTTTCGTAAg < 1:427261/69‑1 (MQ=255)
tGTTCAGCCAGCTTATCGTTGCCTGGCCGTCGGCGATTTCCAGTGGCGCACGGAATACCGTTTCGTAAg < 1:463544/69‑1 (MQ=255)
tGTTCAGCCAGCTTATCGTTGCCTGGCCGTCGGCGATTTCCAGTGGCGCACGGAATACCGTTTCGTAAg < 1:310325/69‑1 (MQ=255)
tGTTCAGCCAGCTTATCGTTGCCTGGCCGTCGGCGATTTCCAGTGGCGCACGGAATACCGTTTCGTAAg < 1:1918642/69‑1 (MQ=255)
tGTTCAGCCAGCTTATCGTTGCCTGGCCGTCGGCGATTTCCAGTGGCGCACGGAATACCGTTTCGTAAg < 1:2109142/69‑1 (MQ=255)
tGTTCAGCCAGCTTATCGTTGCCTGGCCGTCGGCGATTTCCAGTGGCGCACGGAATACCGTTTCGTAAg < 1:2260013/69‑1 (MQ=255)
tGTTCAGCCAGCTTATCGTTGCCTGGCCGTCGGCGATTTCCAGTGGCGCACGGAATACCGTTTCGTAAg < 1:802641/69‑1 (MQ=255)
tGTTCAGCCAGCTTATCGTTGCCTGGCCGTCGGCGATTTCCAGTGGCGCACGGAATACCGTTTCGTAAg < 1:2306608/69‑1 (MQ=255)
tGTTCAGCCAGCTTATCGTTGCCTGGCCGTCGGCGATTTCCAGTGGCGCACGGAATACCGTTTCGTAAg < 1:1473693/69‑1 (MQ=255)
tGTTCAGCCAGCTTATCGTTGCCTGGCCGTCGGCGATTTCCAGTGGCGCACGGAATACCGTTTCGTAAg < 1:1391871/69‑1 (MQ=255)
tGTTCAGCCAGCTTATCGTTGCCTGGCCGTCGGCGATTTCCAGTGGCGCACGGAATACCGTTTCGTAAg < 1:2905816/69‑1 (MQ=255)
gTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGa < 1:2438228/35‑1 (MQ=255)
gTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGa < 1:2301125/35‑1 (MQ=255)
gTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGa < 1:3060812/35‑1 (MQ=255)
gTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGa < 1:1951873/35‑1 (MQ=255)
gTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGa < 1:3131600/35‑1 (MQ=255)
cagccagcTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGGCGCACGGAATACCGTTTCGTAAGGCAt < 1:3309435/69‑1 (MQ=255)
cagccagcTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGGCGCACGGAATACCGTTTCGTAAGGCAt < 1:2448407/69‑1 (MQ=255)
cagccagcTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGGCGCACGGAATACCGTTTCGTAAGGCAt < 1:92705/69‑1 (MQ=255)
agcTTATAGTTGCCTGGCCGTCGTCGATTTCTAGTGGCGCACGGAATACCGTTTCGTAAGGCATCTTTg < 1:3246141/69‑1 (MQ=255)
agcTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGGCGCACGGAATACCGTTTCGTAAGGCATCTTTg < 1:761628/69‑1 (MQ=255)
agcTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGGCGCACGGAATACCGTTTCGTAAGGCATCTTTg < 1:990863/69‑1 (MQ=255)
agcTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGGCGCACGGAATACCGTTTCGTAAGGCATCTTTg < 1:3307531/69‑1 (MQ=255)
agcTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGGCGCACGGAATACCGTTTCGTAAGGCATCTTTg < 1:3007849/69‑1 (MQ=255)
agcTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGGCGCACGGAATACCGTTTCGTAAGGCATCTTTg < 1:2662141/69‑1 (MQ=255)
agcTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGGCGCACGGAATACCGTTTCGTAAGGCATCTTTg < 1:2658128/69‑1 (MQ=255)
agcTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGGCGCACGGAATACCGTTTCGTAAGGCATCTTTg < 1:2535579/69‑1 (MQ=255)
agcTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGGCGCACGGAATACCGTTTCGTAAGGCATCTTTg < 1:2305104/69‑1 (MQ=255)
agcTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGGCGCACGGAATACCGTTTCGTAAGGCATCTTTg < 1:2156489/69‑1 (MQ=255)
agcTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGGCGCACGGAATACCGTTTCGTAAGGCATCTTTg < 1:1470698/69‑1 (MQ=255)
agcTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGGCGCACGGAATACCGTTTCGTAAGGCATCTTTg < 1:1187529/69‑1 (MQ=255)
agcTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGGCGCACGGAATACCGTTTCGTAAGGCATCTTTg < 1:1047671/69‑1 (MQ=255)
ttATAGTTGCCTGGCCGTAGGCGATTTCTAGTGGCGCACgg < 1:455171/41‑1 (MQ=255)
|
TTGGCTTTAACGTCAATATTACGCCCATCCAGCTGGAAACCTTTGTTATTGTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGGCGCACGGAATACCGTTTCGTAAGGCATCTTTG > minE/2210114‑2210240
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
