Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,222,098 |
A→T |
20.3% |
T300T (ACA→ACT) |
accC → |
acetyl‑CoA carboxylase, biotin carboxylase subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,222,098 | 0 | A | T | 20.3%
| 105.9
/ 21.1
| 59 | T300T (ACA→ACT) | accC | acetyl‑CoA carboxylase, biotin carboxylase subunit |
| Reads supporting (aligned to +/- strand): ref base A (22/25); new base T (7/5); total (29/30) |
| Fisher's exact test for biased strand distribution p-value = 5.32e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.71e-01 |
GTTCGAAAACGGCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATCGCTGCCGGTCAACCGCTGTC > minE/2222032‑2222163
|
gTTCGAAAACGGCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAGGTAGAACACCCGGTTACAGa < 1:1126292/69‑1 (MQ=255)
gTTCGAAAACGGCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAGGTAGAACACCCGGTTACAGa < 1:495871/69‑1 (MQ=255)
gTTCGAAAACGGCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAGGTAGAACACCCGGTTACAGa < 1:3315824/69‑1 (MQ=255)
gTTCGAAAACGGCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAGGTAGAACACCCGGTTACAGa < 1:2542474/69‑1 (MQ=255)
gTTCGAAAACGGCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAGGTAGAACACCCGGTTACAGa < 1:2499430/69‑1 (MQ=255)
gTTCGAAAACGGCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAGGTAGAACACCCGGTTACAGa < 1:1392706/69‑1 (MQ=255)
gTTCGAAAACGGCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAGGTAGAACACCCGGTTACAGa < 1:2341364/69‑1 (MQ=255)
gTTCGAAAACGGCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAGGTAGAACACCCGGTTACAGa < 1:2220227/69‑1 (MQ=255)
gTTCGAAAACGGCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAGGTAGAACACCCGGTTACAGa < 1:2012703/69‑1 (MQ=255)
ttCGAAAACGGCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAGGTAGAACACCCGGTTACAGa < 1:98797/68‑1 (MQ=255)
aaaaCGGCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAGGTAGAACACCCGGTTACAGAAATg < 1:1630676/68‑1 (MQ=255)
aaaaCGGCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAGGTAGAACACCCGGTTACAGAAATg < 1:1676233/68‑1 (MQ=255)
aaaaCGGCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAGGTAGAACACCCGGTTACAGAAATg < 1:965375/68‑1 (MQ=255)
aaaaCGGCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAGGTAGAACACCCGGTTACAGAAATg < 1:909010/68‑1 (MQ=255)
aaaaCGGCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAGGTAGAACACCCGGTTACAGAAATg < 1:2695945/68‑1 (MQ=255)
aaaaCGGCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAGGTAGAACACCCGGTTACAGAAATg < 1:1877511/68‑1 (MQ=255)
aaaaCGGCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAGGTAGAACACCCGGTTACAGAAATg < 1:2999803/68‑1 (MQ=255)
aaaaCGGCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAGGTAGAACACCCGGTTACAGAAATg < 1:3075110/68‑1 (MQ=255)
aaaaCGGCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAGGTAGAACACCCGGTTACAGAAATg < 1:397023/68‑1 (MQ=255)
aCGGCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAGGTAGAACACCCGGTTACAGAAATg > 1:986480/1‑65 (MQ=255)
aCGGCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAGGTAGAACACCCGGTTACAGAAATg > 1:3134974/1‑65 (MQ=255)
cgtgTTCTATTTCATCGAAATGAACACCCGTATTCAGGTAGAACACCCGGTTACAGAAATg < 1:3426152/58‑1 (MQ=255)
ttCTATTTCATCGAAATGAACACCCGTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGtt > 1:462951/1‑69 (MQ=255)
ttCTATTTCATCGAAATGAACACCCGTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGtt > 1:884456/1‑69 (MQ=255)
tCTATTTCATCGAAATGAACACCCGTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTg > 1:3410777/1‑69 (MQ=255)
cTATTTCATCGAAATGAACACCCGTATTCAGGTAGAACACCCGGTTACAGAAATg > 1:668650/1‑55 (MQ=255)
cTATTTCATCGAAATGAACACCCGTATTCAGGTAGAACACCCGGTTACAGAAATg > 1:85360/1‑55 (MQ=255)
cTATTTCATCGAAATGAACACCCGTATTCAGGTAGAACACCCGGTTACAGAAATg > 1:875355/1‑55 (MQ=255)
cTATTTCATCGAAATGAACACCCGTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGa > 1:1543685/1‑69 (MQ=255)
cTATTTCATCGAAATGAACACCCGTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGa > 1:2292664/1‑69 (MQ=255)
tCATCGAAATGAACACCCGTATTCAGGTAGAACACCCGGTTACTGAAATGATCACCGGCGTTGAc < 1:1593493/65‑1 (MQ=255)
tCATCGAAATGAACACCCGTATTCAGGTAGAACACCCGGTTACTGAAATGATCACCGGCGTTGAc < 1:2628246/65‑1 (MQ=255)
tCATCGAAATGAACACCCGTATTCAGGTAGAACACCCGGTTACTGAAATGATCACCGGCGTTGAc < 1:1218052/65‑1 (MQ=255)
tCATCGAAATGAACACCCGTATTCAGGTAGAACACCCGGTTACTGAAATGATCACCGGCGTTGAc < 1:1544815/65‑1 (MQ=255)
tCATCGAAATGAACACCCGTATTCAGGTAGAACACCCGGTTACTGAAATGATCACCGGCGTTGAc < 1:1479699/65‑1 (MQ=255)
aaaTGAACACCCGTATTCAGGTAGAACACCCGGTTACAGAAATGATCAcc < 1:619525/50‑1 (MQ=255)
ttCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATCg > 1:1806315/1‑69 (MQ=255)
ttCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATCg > 1:2144010/1‑69 (MQ=255)
ttCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATCg > 1:215931/1‑69 (MQ=255)
aGAACACCCGGTTACTGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATCGCTGc > 1:403797/1‑66 (MQ=255)
aGAACACCCGGTTACTGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATCGCTGc > 1:1580953/1‑66 (MQ=255)
aGAACACCCGGTTACTGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATCGCTGc > 1:1763119/1‑66 (MQ=255)
aGAACACCCGGTTACTGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATCGCTGc > 1:624258/1‑66 (MQ=255)
aGAACACCCGGTTACTGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATCGCTGc > 1:2160413/1‑66 (MQ=255)
aGAACACCCGGTTACTGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATCGCTGc > 1:2275258/1‑66 (MQ=255)
aGAACACCCGGTTACTGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATCGCTGc > 1:2283724/1‑66 (MQ=255)
acCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATCGCTGCCGGTCAAc < 1:3359791/69‑1 (MQ=255)
acCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATCGCTGCCGGTCAAc < 1:113156/69‑1 (MQ=255)
acCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATCGCTGCCGGTCAAc < 1:8640/69‑1 (MQ=255)
acCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATCGCTGCCGGTCAAc < 1:2761091/69‑1 (MQ=255)
tACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATCGCTGCCGGTCAACCGCTGTc > 1:2578053/1‑69 (MQ=255)
tACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATCGCTGCCGGTCAACCGCTGTc > 1:1175655/1‑69 (MQ=255)
tACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATCGCTGCCGGTCAACCGCTGTc > 1:2081371/1‑69 (MQ=255)
tACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATCGCTGCCGGTCAACCGCTGTc > 1:2420004/1‑69 (MQ=255)
tACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATCGCTGCCGGTCAACCGCTGTc > 1:1885500/1‑69 (MQ=255)
tACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATCGCTGCCGGTCAACCGCTGTc > 1:2730798/1‑69 (MQ=255)
tACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATCGCTGCCGGTCAACCGCTGTc > 1:173666/1‑69 (MQ=255)
tACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATCGCTGCCGGTCAACCGCTGTc > 1:1296764/1‑69 (MQ=255)
tACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATCGCTGCCGGTCAACCGCTGTc > 1:1123217/1‑69 (MQ=255)
|
GTTCGAAAACGGCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATCGCTGCCGGTCAACCGCTGTC > minE/2222032‑2222163
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
