Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F1 I0 R1 4577 57.4 3436498 77.7% 2670158 63.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,282,451 A→G 24.0% L285L (CTA→CTG)  sthA → pyridine nucleotide transhydrogenase, soluble

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,282,4510AG24.0% 147.8 / 40.4 118L285L (CTA→CTG) sthApyridine nucleotide transhydrogenase, soluble
Reads supporting (aligned to +/- strand):  ref base A (60/29);  new base G (11/17);  total (71/47)
Fisher's exact test for biased strand distribution p-value = 1.37e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 124 total aligned reads displayed.

TGCTCTATGCCAACGGTCGCACCGGTAATACCGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCATGTATCAGACCGCACAGCCACACGTTTACGC  >  minE/2282390‑2282519
                                                             |                                                                    
tgcTCTATGCCAACGGTCGCACCGGTAATACCGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACt                                                                <  1:1121325/68‑1 (MQ=255)
tgcTCTATGCCAACGGTCGCACCGGTAATACCGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACt                                                                <  1:189039/68‑1 (MQ=255)
tgcTCTATGCCAACGGTCGCACCGGTAATACCGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACt                                                                >  1:1620422/1‑68 (MQ=255)
tgcTCTATGCCAACGGTCGCACCGGTAATACCGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACt                                                                <  1:2603054/68‑1 (MQ=255)
tgcTCTATGCCAACGGTCGCACCGGTAATACCGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACt                                                                <  1:2746941/68‑1 (MQ=255)
tgcTCTATGCCAACGGTCGCACCGGTAATACCGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACt                                                                <  1:2894626/68‑1 (MQ=255)
tgcTCTATGCCAACGGTCGCACCGGTAATACCGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACt                                                                >  1:817825/1‑68 (MQ=255)
tgcTCTATGCCAACGGTCGCACCGGTAATACCGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACt                                                                <  1:94930/68‑1 (MQ=255)
      aTGCCAACGGTCGCACCGGTAATACCGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACt                                                                <  1:2482565/62‑1 (MQ=255)
         ccAACGGTCGCACCGGTAATACCGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCcgcg                                                      >  1:3350507/1‑69 (MQ=255)
          cAACGGTCGCACCGGTAATACCGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCcgcg                                                      <  1:1909394/68‑1 (MQ=255)
                    aCCGGTAATACCGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAg                                           <  1:577063/69‑1 (MQ=255)
                             aCCGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACa                                    >  1:2120193/1‑67 (MQ=255)
                             aCCGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAg                                   >  1:386604/1‑68 (MQ=255)
                             aCCGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAg                                   >  1:207475/1‑68 (MQ=255)
                             aCCGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGc                                  >  1:1942940/1‑69 (MQ=255)
                             aCCGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGc                                  >  1:983093/1‑69 (MQ=255)
                             aCCGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGc                                  >  1:1817058/1‑69 (MQ=255)
                              ccGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACt                                                                >  1:2115152/1‑38 (MQ=255)
                              ccGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACt                                                                >  1:1371503/1‑38 (MQ=255)
                              ccGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACt                                                                >  1:2662204/1‑38 (MQ=255)
                              ccGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACt                                                                >  1:2406870/1‑38 (MQ=255)
                              ccGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACt                                                                >  1:3004233/1‑38 (MQ=255)
                              ccGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACt                                                                >  1:560409/1‑38 (MQ=255)
                              ccGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACt                                                                >  1:564338/1‑38 (MQ=255)
                              ccGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACt                                                                >  1:1024597/1‑38 (MQ=255)
                              ccGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACt                                                                >  1:1184943/1‑38 (MQ=255)
                              ccGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACt                                                                >  1:199436/1‑38 (MQ=255)
                              ccGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCa                                 >  1:3135152/1‑69 (MQ=255)
                              ccGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCa                                 >  1:2609803/1‑69 (MQ=255)
                              ccGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCa                                 >  1:1123254/1‑69 (MQ=255)
                              ccGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCa                                 <  1:910484/69‑1 (MQ=255)
                              ccGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCa                                 >  1:2719383/1‑69 (MQ=255)
                              ccGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCa                                 >  1:283333/1‑69 (MQ=255)
                              ccGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCa                                 >  1:3125284/1‑69 (MQ=255)
                              ccGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCa                                 >  1:3138336/1‑69 (MQ=255)
                              ccGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCa                                 >  1:1266806/1‑69 (MQ=255)
                              ccGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCa                                 >  1:713121/1‑69 (MQ=255)
                              ccGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCa                                 >  1:1819636/1‑69 (MQ=255)
                              ccGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCa                                 >  1:642735/1‑69 (MQ=255)
                              ccGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCa                                 >  1:119065/1‑69 (MQ=255)
                              ccGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCa                                 >  1:577187/1‑69 (MQ=255)
                              ccGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCa                                 >  1:630142/1‑69 (MQ=255)
                              ccGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCa                                 <  1:1372550/69‑1 (MQ=255)
                              ccGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCa                                 <  1:1791002/69‑1 (MQ=255)
                              ccGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCa                                 >  1:1699602/1‑69 (MQ=255)
                              ccGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCa                                 >  1:1923446/1‑69 (MQ=255)
                              ccGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCa                                 <  1:1573766/69‑1 (MQ=255)
                              ccGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCa                                 >  1:2003974/1‑69 (MQ=255)
                              ccGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCa                                 >  1:1472304/1‑69 (MQ=255)
                              ccGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCa                                 >  1:2296181/1‑69 (MQ=255)
                              ccGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCa                                 >  1:2446948/1‑69 (MQ=255)
                              ccGATTCGCTGGCGTTACAGAACAATGGGCTAGAAACTCACAGCCGCGGACAGCTGAAGGTCAACAGCa                                 >  1:366670/1‑69 (MQ=255)
                               cGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCa                                 <  1:824249/68‑1 (MQ=255)
                                gATTCGCTGGCGTTACAGAACATTGGGCTGGAAACTGACAGTCGCGGACAGCTGAAGGTCCACAGCAt                                >  1:2244776/1‑68 (MQ=255)
                                gATTCGCTGGCGTTACAGAACATTGGGCTGGAAACTGACAGTCGCGGACAGCTGAAGGTCAACAGCATg                               >  1:3343041/1‑69 (MQ=255)
                                gATTCGCTGGCGTTACAGAACATTGGGCTGGAAACTGACAGTCGCGGACAGCTGAAGGTCAACAGCATg                               >  1:3319323/1‑69 (MQ=255)
                                gATTCGCTGGCGTTACAGAACATTGGGCTGGAAACTGACAGTCGCGGACAGCTGAAGGTCAACAGCATg                               >  1:2223692/1‑69 (MQ=255)
                                gATTCGCTGGCGTTACAGAACATTGGGCTGGAAACTGACAGTCGCGGACAGCTGAAGGTCAACAGCATg                               >  1:749776/1‑69 (MQ=255)
                                gATTCGCTGGCGTTACAGAACATTGGGCTGGAAACTGACAGTCGCGGACAGCTGAAGGTCAACAGCATg                               >  1:691075/1‑69 (MQ=255)
                                gATTCGCTGGCGTTACAGAACATTGGGCTGGAAACTGACAGTCGCGGACAGCTGAAGGTCAACAGCATg                               >  1:3016988/1‑69 (MQ=255)
                                gATTCGCTGGCGTTACAGAACATTGGGCTGGAAACTGACAGTCGCGGACAGCTGAAGATCAACAGCATg                               >  1:3387132/1‑69 (MQ=255)
                                gATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGTCGCGGACAGCTGAAGGTCAACAGCATg                               >  1:2645496/1‑69 (MQ=255)
                                gATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCAtt                               <  1:3122315/69‑2 (MQ=255)
                                gATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCATg                               >  1:2017450/1‑69 (MQ=255)
                                gATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCATg                               <  1:2328867/69‑1 (MQ=255)
                                 aTTCGCTGGCGTTACAGAACATTGGGCTTGAAACTGACAGTCGCGGACAGCTGAAGGTCAACAGCAt                                <  1:2719587/67‑1 (MQ=255)
                                 aTTCGCTGGCGTTACAGAACATTGGGCTGGAAACTGACAGTCGCGGACAGCTGAAGGTCAACAGCAt                                <  1:69201/67‑1 (MQ=255)
                                 aTTCGCTGGCGTTACAGAACATTGGGCTGGAAACTGACAGTCGCGGACAGCTGAAGGTCAACAGCAt                                <  1:722281/67‑1 (MQ=255)
                                 aTTCGCTGGCGTTACAGAACATTGGGCTGGAAACTGACAGTCGCGGACAGCTGAAGGTCAACAGCAt                                <  1:1953505/67‑1 (MQ=255)
                                 aTTCGCTGGCGTTACAGAACATTGGGCTGGAAACTGACAGTCGCGGACAGCTGAAGGTCAACAGCAt                                <  1:49989/67‑1 (MQ=255)
                                 aTTCGCTGGCGTTACAGAACATTGGGCTGGAAACTGACAGTCGCGGACAGCTGAAGGTCAACAGCAt                                <  1:1574590/67‑1 (MQ=255)
                                 aTTCGCTGGCGTTACAGAACATTGGGCTGGAAACTGACAGTCGCGGACAGCTGAAGGTCAACAGCAt                                <  1:2719716/67‑1 (MQ=255)
                                 aTTCGCTGGCGTTACAGAACATTGGGCTGGAAACTGACAGTCGCGGACAGCTGAAGGTCAACAGCAt                                <  1:2132150/67‑1 (MQ=255)
                                 aTTCGCTGGCGTTACAGAACATTGGGCTGGAAACTGACAGTCGCGGACAGCTGAAGGTCAACAGCAt                                <  1:3330031/67‑1 (MQ=255)
                                 aTTCGCTGGCGTTACAGAACATTGGGCTGGAAACTGACAGTCGCGGACAGCTGAAGGTCAACAGCAt                                <  1:2769052/67‑1 (MQ=255)
                                 aTTCGCTGGCGTTACAGAACATTGGGCTGGAAACTGACAGTCGCGGACAGCTGAAGGTCAACAGCAt                                <  1:2850878/67‑1 (MQ=255)
                                 aTTCGCTGGCGTTACAGAACATTGGGCTGGAAACTGACAGTCGCGGACAGCTGAAGGTCAACAGCAt                                <  1:3250217/67‑1 (MQ=255)
                                 aTTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCATg                               <  1:777012/68‑1 (MQ=255)
                                 aTTCGCTGGCGTTACAGAACATT‑GGCTGGAAACTGACAGTCGCGGACAGCTGAAGGTCAACAGCAt                                <  1:244392/66‑1 (MQ=255)
                                  ttCGCTGGCGTTACAGAACATTGGGCTGGAAACTGACAGTCGCGGACAGCTGAAGGTCAACAGCAt                                <  1:1442045/66‑1 (MQ=255)
                                         gCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCATGTATCAGAc                       >  1:2552723/1‑68 (MQ=255)
                                         gCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCATGTATCAGAc                       >  1:2873277/1‑68 (MQ=255)
                                         gCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCATGTATCAGAc                       >  1:2978637/1‑68 (MQ=255)
                                         gCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCATGTATCAGAc                       >  1:631828/1‑68 (MQ=255)
                                         gCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCATGTATCAGAc                       >  1:627970/1‑68 (MQ=255)
                                         gCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCATGTATCAGAc                       >  1:2678597/1‑68 (MQ=255)
                                         gCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCATGTATCAGAc                       >  1:401456/1‑68 (MQ=255)
                                         gCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCATGAATCAGAc                       >  1:2209255/1‑68 (MQ=255)
                                             tACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCATGTATCAGACCGcac                  <  1:2361540/69‑1 (MQ=255)
                                             tACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCATGTATCAGACCGcac                  <  1:1234773/69‑1 (MQ=255)
                                              aCAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCATGTATCAGACCGcac                  <  1:864237/68‑1 (MQ=255)
                                                    cATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCATGTATCAGACCGCACAGCcaca           >  1:129210/1‑69 (MQ=255)
                                                    cATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCATGTATCAGACCGCACAGCcaca           >  1:1349185/1‑69 (MQ=255)
                                                      ttGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCATGTATCAGACCGCACAGCCACACg         <  1:202845/69‑1 (MQ=255)
                                                        gggCTAGAAACTGACAGCCGCGGACAGCTGAAGGTAAACAGCATGTATCAGACCGCACAGCCACACGtt       <  1:306008/69‑1 (MQ=255)
                                                             aGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCATGTATCAGACCGCACAGCCACACGTTTAcgc  <  1:2664898/69‑1 (MQ=255)
                                                             aGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCATGTATCAGACCGCACAGCCACACGTTTAcgc  <  1:3298825/69‑1 (MQ=255)
                                                             aGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCATGTATCAGACCGCACAGCCACACGTTTAcgc  <  1:404260/69‑1 (MQ=255)
                                                             aGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCATGTATCAGACCGCACAGCCACACGTTTAcgc  <  1:3369881/69‑1 (MQ=255)
                                                             |                                                                    
TGCTCTATGCCAACGGTCGCACCGGTAATACCGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCATGTATCAGACCGCACAGCCACACGTTTACGC  >  minE/2282390‑2282519

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A