Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,292,688 |
A→T |
58.2% |
intergenic (+239/‑182) |
fsaB → / → gldA |
fructose‑6‑phosphate aldolase 2/glycerol dehydrogenase, NAD |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,292,688 | 0 | A | T | 58.2%
| 20.0
/ 63.9
| 67 | intergenic (+239/‑182) | fsaB/gldA | fructose‑6‑phosphate aldolase 2/glycerol dehydrogenase, NAD |
| Reads supporting (aligned to +/- strand): ref base A (15/13); new base T (13/26); total (28/39) |
| Fisher's exact test for biased strand distribution p-value = 1.33e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.00e-01 |
TCGTACGGTTCAGGAGCTGCAAACGCTGTTAGAAATGCACGCGCCAGAAAGCATGGTGCTGGCAGCCAGCTTTAAAACGCCGCGTCAGGCGCTGGACTGTTTACTGGCAGGATGTGAATCCATCACCCTGCC > minE/2292625‑2292756
|
tCGTACGGTTCAGGAGCTGCAAACGCTGTTAGAAATGCACGCGCCAGAAAGCATGGTGCTGGcagccag < 1:664749/69‑1 (MQ=255)
cGTACGGTTCAGGAGCTGCAAACGCTGTTAGAAATGCACGCGCCAGAAAGCATGGTTCTGGcagccagc > 1:3395390/1‑69 (MQ=255)
cGTACGGTTCAGGAGCTGCAAACGCTGTTAGAAATGCACGCGCCAGAAAGCATGGTGCTGGcagccagc > 1:793369/1‑69 (MQ=255)
cGTACGGTTCAGGAGCTGCAAACGCTGTTAGAAATGCACGCGCCAGAAAGCATGGTGCTGGcagccagc > 1:1827185/1‑69 (MQ=255)
cGTACGGTTCAGGAGCTGCAAACGCTGTTAGAAATGCACGCGCCAGAAAGCATGGTGCTGGcagccagc > 1:1848194/1‑69 (MQ=255)
cGTACGGTTCAGGAGCTGCAAACGCTGTTAGAAATGCACGCGCCAGAAAGCATGGTGCTGGcagccagc > 1:729822/1‑69 (MQ=255)
cGTACGGTTCAGGAGCTGCAAACGCTGTTAGAAATGCACGCGCCAGAAAGCATGGTGCTGGcagccagc < 1:2349316/69‑1 (MQ=255)
cGTACGGTTCAGGAGCTGCAAACGCTGTTAGAAATGCACGCGCCAGAAAGCATGGTGCTGGcagccagc > 1:1526819/1‑69 (MQ=255)
cGTACGGTTCAGGAGCTGCAAACGCTGTTAGAAATGCACGCGCCAGAAAGCATGGTGCTGGcagccagc < 1:60902/69‑1 (MQ=255)
cGTACGGTTCAGGAGCTGCAAACGCTGTTAGAAATGCACGCGCCAGAAAGCATGGTGCTGGcagccagc > 1:2844412/1‑69 (MQ=255)
cGTACGGTTCAGGAGCTGCAAACGCTGTTAGAAATGCACGCGCCAGAAAGCATGGTGCTGGcagccagc > 1:2882748/1‑69 (MQ=255)
cGTACGGTTCAGGAGCTGCAAACGCTGTTAGAAATGCACGCGCCAGAAAGCATGGTGCTGGcagccagc > 1:2935276/1‑69 (MQ=255)
cGTACGGTTCAGGAGCTGCAAACGCTGTTAGAAATGCACGCGCCAGAAAGCATGGTGCTGGcagccagc > 1:544360/1‑69 (MQ=255)
cGTACGGTTCAGGAGCTGCAAACGCTGTTAGAAATGCACGCGCCAGAAAGCATGGTGCTGGcagccagc > 1:539981/1‑69 (MQ=255)
cGTACGGTTCAGGAGCTGCAAACGCTGTTAGAAATGCACGCGCCAGAAAGCATGGTGCTGGcagccagc < 1:472060/69‑1 (MQ=255)
cGTACGGTTCAGGAGCTGCAAACGCTGTTAGAAATGCACGCGCCAGAAAGCATGGTGCTGGcagccagc > 1:436781/1‑69 (MQ=255)
cGTACGGTTCAGGAGCTGCAAACGCTGTTAGAAATGCACGCGCCAGAAAGCATGGTGCTGGcagccagc > 1:383536/1‑69 (MQ=255)
ggAGTTGCAAGCGTTACTGGAAATGCATGCGCCAGAAAGCATGGTGCTGGCTGCCAGCTTTAAAAcacc > 1:2362998/1‑69 (MQ=255)
ggAGTTGCAAGCGTTACTGGAAATGCATGCGCCAGAAAGCATGGTGCTGGCTGCCAGCTTTAAAAcacc > 1:2347231/1‑69 (MQ=255)
ggAGTTGCAAGCGTTACTGGAAATGCATGCGCCAGAAAGCATGGTGCTGGCTGCCAGCTTTAAAAcacc > 1:1864136/1‑69 (MQ=255)
ggAGTTGCAAGCGTTACTGGAAATGCATGCGCCAGAAAGCATGGTGCTGGCTGCCAGCTTTAAAAcacc > 1:2284239/1‑69 (MQ=255)
ggAGTTGCAAGCGTTACTGGAAATGCATGCGCCAGAAAGCATGGTGCTGGCTGCCAGCTTTAAAAcacc > 1:1101255/1‑69 (MQ=255)
ggAGTTGCAAGCGTTACTGGAAATGCATGCGCCAGAAAGCATGGTGCTGGCTGCCAGCTTTAAAAcacc > 1:1493469/1‑69 (MQ=255)
ggAGTTGCAAGCGTTACTGGAAATGCATGCGCCAGAAAGCATGGTGCTGGCTGCCAGCTTTAAAAcacc > 1:1209629/1‑69 (MQ=255)
ggAGTTGCAAGCGTTACTGGAAATGCATGCGCCAGAAAGCATGGTGCTGGCTGCCAGCTTTAAAAcacc > 1:1568697/1‑69 (MQ=255)
ggAGTTGCAAGCGTTACTGGAAATGCATGCGCCAGAAAGCATGGTGCTGGCTGCCAGCTTTAAAAcacc > 1:1692895/1‑69 (MQ=255)
ggAGTTGCAAGCGTTACTGGAAATGCATGCGCCAGAAAGCATGGTGCTGGCTGCCAGCTTTAAAAcacc > 1:1668258/1‑69 (MQ=255)
ggAGTTGCAAGCGTTACTGGAAATGCATGCGCCAGAAAGCATGGTGCTGGCTGCCAGCTTTAAAAcacc > 1:1162700/1‑69 (MQ=255)
ggAGTTGCAAGCGTTACTGGAAATGCATGCGCCAGAAAGCATGGTGCTGGCTGCCAGCTTTAAAAcac > 1:953027/1‑66 (MQ=255)
ggAGTTGCAAGCGTTACTGGAAATGCATGCGCAAGAAAGCATGGTGCTGGCTGCCAGCTTTAAAAcac > 1:2794384/1‑66 (MQ=255)
aaCGCTGTTAGAAATGCACGCGCCAGAAAGCATGGTGCTGGCAGCCAGCTTTaa > 1:1043137/1‑54 (MQ=255)
aGAAATGCACGCGCCAGAAAGCATGGTGCTGGCAGCCAGCTTTAAAACGCCGCGTCAGGCGCTGGACTg > 1:327913/1‑69 (MQ=255)
aGAAATGCACGCGCCAGAAAGCATGGTGCTGGCAGCCAGCTTTAAAACGCCGCGTCAGGCGCTGGACTg > 1:676729/1‑69 (MQ=255)
aGAAATGCACGCGCCAGAAAGCATGGTGCTGGCAGCCAGCTTTAAAACGCCGCGTCAGGCGCTGGACTg > 1:1433290/1‑69 (MQ=255)
aGAAATGAACGCGCCAGAAAGCATGGTGCTGGCAGCCAGCTTTAAAACGCCGCGTCAGGCGCTGGACTg > 1:1608528/1‑69 (MQ=255)
aaTGCACGCGCCAGAAAGGATGGTGCTGGCAGCCAGCTTTAAAACGCCGCGGCAGGCGCTGGACTGtt < 1:1030740/68‑1 (MQ=255)
aaTGCACGCGCCAGAAAGCATGGTGCTGGCAGCCAGCTTTAAAACGCCGCGTCAGGCGCTGGACTGtt < 1:3291755/68‑1 (MQ=255)
aaTGCACGCGCCAGAAAGCATGGTGCTGGCAGCCAGCTTTAAAACGCCGCGTCAGGCGCTGGACTGtt < 1:779308/68‑1 (MQ=255)
aaTGCACGCGCCAGAAAGCATGGTGCTGGCAGCCAGCTTTAAAACGCCGCGTCAGGCGCTGGACTGtt < 1:298829/68‑1 (MQ=255)
aaTGCACGCGCCAGAAAGCATGGTGCTGGCAGCCAGCTTTAAAACGCCGCGTCAGGCGCTGGACTGtt < 1:3319715/68‑1 (MQ=255)
aaTGCACGCGCCAGAAAGCATGGTGCTGGCAGCCAGCTTTAAAACGCCGCGTCAGGCGCTGGACTGtt < 1:2956745/68‑1 (MQ=255)
aaTGCACGCGCCAGAAAGCATGGTGCTGGCAGCCAGCTTTAAAACGCCGCGTCAGGCGCTGGACTGtt < 1:1890978/68‑1 (MQ=255)
aaTGCACGCGCCAGAAAGCATGGTGCTGGCAGCCAGCTTTAAAACGCCGCGTCAGGCGCTGGACTGtt < 1:2689710/68‑1 (MQ=255)
aaTGCACGCGCCAGAAAGCATGGTGCTGGCAGCCAGCTTTAAAACGCCGCGTCAGGCGCTGGACTGtt < 1:2774470/68‑1 (MQ=255)
tGCACGCGCCAGAAAGCATGGTGCTGGCAGCCAGCTTTAAAACGCCGCGTCAGGCGCTGGACTGTTTAc < 1:1175372/69‑1 (MQ=255)
tGCACGCGCCAGAAAGCATGGTGCTGGCAGCCAGCTTTAAAACGCCGCGTCAGGCGCTGGACTGTTTAc > 1:908755/1‑69 (MQ=255)
gCACGCGCCAGAAAGCATGGTGCTGGCAGCCAGCTTTAAAACGCCGCGTCAGGCGCTGGACTGTTTACt > 1:557130/1‑69 (MQ=255)
gCACGCGCCAGAAAGCATGGTGCTGGCAGCCAGCTTTAAAACGCCGCGTCAGGCGCTGGACTGTTTACt > 1:2927365/1‑69 (MQ=255)
gCACGCGCCAGAAAGCATGGTGCTGGCAGCCAGCTTTAAAACGCCGCGTCAGGCGCTGGACTGTTTACt > 1:632451/1‑69 (MQ=255)
gCACGCGCCAGAAAGCATGGTGCTGGCAGCCAGCTTTAAAACGCCGCGTCAGGCGCTGGACTGTTTACt > 1:2422347/1‑69 (MQ=255)
gCACGCGCCAGAAAGCATGGTGCTGGCAGCCAGCTTTAAAACGCCGCGTCAGGCGCTGGACTGTTTACt > 1:2063894/1‑69 (MQ=255)
gCACGCGCCAGAAAGCATGGTGCTGGCAGCCAGCTTTAAAACGCCGCGTCAGGCGCTGGACTGTTTACt > 1:2106674/1‑69 (MQ=255)
gCACGCGCCAGAAAGCATGGTGCTGGCAGCCAGCTTTAAAACGCCGCGTCAGGCGCTGGACTGTTTACt > 1:2283472/1‑69 (MQ=255)
gCACGCGCCAGAAAGCATGGTGCTGGCAGCCAGCTTTAAAACGCCGCGTCAGGCGCTGGACTGTTTACt > 1:415729/1‑69 (MQ=255)
gCACGCGCCAGAAAGCATGGTGCTGGCAGCCAGCTTTAAAACGCCGCGTCAGGCGATGGACTGttt > 1:2532585/1‑66 (MQ=255)
aCGCGCCAGAAAGCATGGTGCTGGCAGCCAGCTTTAAAACGCCGCGTCAGGCGCTGGACTGtt < 1:2278005/63‑1 (MQ=255)
tgcgcCAGAAAGCTTGGTGCTGGCTGCCAGCTTTATAACACCACGTCAGGCGCTGGATTGTTTGCTGGc < 1:1190198/68‑1 (MQ=21)
tgcgcCAGAAAGCATGGTGCTGGCTGCCAGCTTTAAAACACCACGTCAGGCGCTGGATTGTTTGCTGGc < 1:883251/68‑1 (MQ=25)
tgcgcCAGAAAGCATGGTGCTGGCTGCCAGCTTTAAAACACCACGTCAGGCGCTGGATTGTTTGCTGGc < 1:912062/68‑1 (MQ=25)
tgcgcCAGAAAGCATGGTGCTGGCTGCCAGCTTTAAAACACCACGTCAGGCGCTGGATTGTTTGCTGGc < 1:991930/68‑1 (MQ=25)
tgcgcCAGAAAGCATGGTGCTGGCTGCCAGCTTTAAAACACCACGTCAGGCGCTGGATTGTTTGCTGGc < 1:719790/68‑1 (MQ=25)
tgcgcCAGAAAGCATGGTGCTGGCTGCCAGCTTTAAAACACCACGTCAGGCGCTGGATTGTTTGCTGGc < 1:607024/68‑1 (MQ=25)
tgcgcCAGAAAGCATGGTGCTGGCTGCCAGCTTTAAAACACCACGTCAGGCGCTGGATTGTTTGCTGGc < 1:976558/68‑1 (MQ=25)
tgcgcCAGAAAGCATGGTGCTGGCTGCCAGCTTTAAAACACCACGTCAGGCGCTGGATTGTTTGCTGGc < 1:3230551/68‑1 (MQ=25)
tgcgcCAGAAAGCATGGTGCTGGCTGCCAGCTTTAAAACACCACGTCAGGCGCTGGATTGTTTGCTGGc < 1:130427/68‑1 (MQ=25)
tgcgcCAGAAAGCATGGTGCTGGCTGCCAGCTTTAAAACACCACGTCAGGCGCTGGATTGTTTGCTGGc < 1:1484610/68‑1 (MQ=25)
tgcgcCAGAAAGCATGGTGCTGGCTGCCAGCTTTAAAACACCACGTCAGGCGCTGGATTGTTTGCTGGc < 1:1543156/68‑1 (MQ=25)
tgcgcCAGAAAGCATGGTGCTGGCTGCCAGCTTTAAAACACCACGTCAGGCGCTGGATTGTTTGCTGGc < 1:1710333/68‑1 (MQ=25)
tgcgcCAGAAAGCATGGTGCTGGCTGCCAGCTTTAAAACACCACGTCAGGCGCTGGATTGTTTGCTGGc < 1:1752929/68‑1 (MQ=25)
tgcgcCAGAAAGCATGGTGCTGGCTGCCAGCTTTAAAACACCACGTCAGGCGCTGGATTGTTTGCTGGc < 1:1777589/68‑1 (MQ=25)
tgcgcCAGAAAGCATGGTGCTGGCTGCCAGCTTTAAAACACCACGTCAGGCGCTGGATTGTTTGCTGGc < 1:2086639/68‑1 (MQ=25)
tgcgcCAGAAAGCATGGTGCTGGCTGCCAGCTTTAAAACACCACGTCAGGCGCTGGATTGTTTGCTGGc < 1:2108534/68‑1 (MQ=25)
tgcgcCAGAAAGCATGGTGCTGGCTGCCAGCTTTAAAACACCACGTCAGGCGCTGGATTGTTTGCTGGc < 1:2373826/68‑1 (MQ=25)
tgcgcCAGAAAGCATGGTGCTGGCTGCCAGCTTTAAAACACCACGTCAGGCGCTGGATTGTTTGCTGGc < 1:2532134/68‑1 (MQ=25)
tgcgcCAGAAAGCATGGTGCTGGCTGCCAGCTTTAAAACACCACGTCAGGCGCTGGATTGTTTGCTGGc < 1:2560783/68‑1 (MQ=25)
tgcgcCAGAAAGCATGGTGCTGGCTGCCAGCTTTAAAACACCACGTCAGGCGCTGGATTGTTTGCTGGc < 1:3093352/68‑1 (MQ=25)
tgcgcCAGAAAGCATGGTGCTGGCTGCCAGCTTTAAAACACCACGTCAGGCGCTGGATTGTTTGCTGGc < 1:3127883/68‑1 (MQ=25)
tgcgcCAGAAAGCATGGTGCTGGCTGCCAGCTTTAAAACACCACGTCAGGCGCTGGATTGTTTGCTGGc < 1:3325272/68‑1 (MQ=25)
tgcgcCAGAAAGCATGGTGCTGGCTGCCAGCTTTAAAAAACCACGTCAGGCGCTGGATTGTTTGCTGGc < 1:2638173/68‑1 (MQ=25)
gcgcCAGAAAGCTTGGTGCTGGCTGCCAGCTTTAAAACACCACGTCAGGCGCTGGATTGTTTGCTGGc < 1:3119480/68‑1 (MQ=21)
gcgcCAGAAAGCATGGTGCTGGCTGCCAGCTTTAAAACACCACGTCAGGCGCTGGATTGTTTGCTGGc < 1:3086742/68‑1 (MQ=25)
gcgcCAGAAAGCATGGTGCTGGCTGCCAGCTTTAAAACACCACGTCAGGCGCTGGATTGTTTGCTGGc < 1:1694458/68‑1 (MQ=25)
cAGAAAGCATGGTGCTGGCAGCCAGCTTTAAAACGCCGCGTCAGGCGCTGGACTGTTTACTGGCAGGAt < 1:2594532/69‑1 (MQ=255)
cAGAAAGCATGGTGCTGGCAGCCAGCTTTAAAACGCCGCGTCAGGCGCTGGACTGTTTACTGGCAGGAt < 1:2781200/69‑1 (MQ=255)
agccagcTTTAAAACGCCGCGTCAGGCGCTGGACTGTTTACTGGCAGGATGTGAATCCATCACCCTGcc < 1:2508425/69‑1 (MQ=255)
|
TCGTACGGTTCAGGAGCTGCAAACGCTGTTAGAAATGCACGCGCCAGAAAGCATGGTGCTGGCAGCCAGCTTTAAAACGCCGCGTCAGGCGCTGGACTGTTTACTGGCAGGATGTGAATCCATCACCCTGCC > minE/2292625‑2292756
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
