Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F1 I0 R1 4577 57.4 3436498 77.7% 2670158 63.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,292,956 A→G 50.8% A29A (GCA→GCG)  gldA → glycerol dehydrogenase, NAD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,292,9560AG50.8% 1.6 / 126.5 136A29A (GCA→GCG) gldAglycerol dehydrogenase, NAD
Reads supporting (aligned to +/- strand):  ref base A (32/35);  new base G (55/14);  total (87/49)
Fisher's exact test for biased strand distribution p-value = 1.52e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 137 total aligned reads displayed.

CACCGGGTAAATACATCCAGGGCGCTGATGTGATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAGTGGTGGGTGACAAATTTGTTTTAGGTTTTGCTCAATCCACTGTCGAGAAAAG  >  minE/2292889‑2293021
                                                                   |                                                                 
cACCGGGTAAATACATCCAGGGCGCTGATGTGATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAg                                                                  >  1:2293560/1‑69 (MQ=255)
cACCGGGTAAATACATCCAGGGCGCTGATGTGATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAg                                                                  >  1:874063/1‑69 (MQ=255)
      gTAAATACATCCAGGGCGCTGATGTGATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCt                                                            >  1:2841408/1‑69 (MQ=255)
       tAAATACATCCAGGGCGCTGATGTGATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTg                                                           >  1:1767340/1‑69 (MQ=255)
           tACATCCAGGGCGCTGATGTGATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGtt                                                        <  1:1973186/68‑1 (MQ=255)
           tACATCCAGGGCGCTGATGTGATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGtt                                                        <  1:3024846/68‑1 (MQ=255)
             cATCCAGGGCGCTGATGTGATTAATCGTCTTGGCGAATACCTGAAGCCTCTGGCAGAACGCTGGTTAgt                                                     <  1:1583326/69‑1 (MQ=255)
             cATCCAGGGCGCTGATGTGATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAgt                                                     <  1:828978/69‑1 (MQ=255)
             cATCCAGGGCGCTGATGTGATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAgt                                                     <  1:2880791/69‑1 (MQ=255)
             cATCCAGGGCGCTGATGTGATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAgt                                                     <  1:1657349/69‑1 (MQ=255)
             cATCCAGGGCGCTGATGTGATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAgt                                                     <  1:3431756/69‑1 (MQ=255)
                ccAGGGCGCTGATGTGATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAgtggt                                                  <  1:3285427/69‑1 (MQ=255)
                ccAGGGCGCTGATGTGATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAgtggt                                                  <  1:2858857/69‑1 (MQ=255)
                ccAGGGCGCTGATGTGATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAgtggt                                                  <  1:2088787/69‑1 (MQ=255)
                ccAGGGCGCTGATGTGATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAgtggt                                                  <  1:1905826/69‑1 (MQ=255)
                ccAGGGCGCTGATGTGATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAgtggt                                                  <  1:1013713/69‑1 (MQ=255)
                ccAGGGCGCTGATGTGATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAgtggt                                                  <  1:3007164/69‑1 (MQ=255)
                ccAGGGCGCTGATGTGATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAgtggt                                                  <  1:577405/69‑1 (MQ=255)
                 cAGGGCGCTGATGTGATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAg                                                                  <  1:1145431/52‑1 (MQ=255)
                 cAGGGCGCTGATGTGATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAg                                                                  <  1:1867118/52‑1 (MQ=255)
                 cAGGGCGCTGATGTGATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAg                                                                  <  1:767971/52‑1 (MQ=255)
                 cAGGCCGCTGATGTGATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAg                                                                  <  1:2647175/52‑1 (MQ=255)
                  aGGGCGCTGATGTGATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAg                                                                  <  1:236127/51‑1 (MQ=255)
                  aGGGCGCAGATGTAATTAATCGTCTGGGCGAGTACCTGAAGCCACTGGCGGAACGCTGGTTAGtggtgg                                                >  1:588443/1‑69 (MQ=255)
                  aGGGCGCAGATGTAATTAATCGTCTGGGCGAGTACCTGAAGCCACTGGCGGAACGCTGGTTAGtggtgg                                                >  1:1924559/1‑69 (MQ=255)
                  aGGGCGCAGATGTAATTAATCGTCTGGGCGAGTACCTGAAGCCACTGGCGGAACGCTGGTTAGtggtgg                                                >  1:3324892/1‑69 (MQ=255)
                  aGGGCGCAGATGTAATTAATCGTCTGGGCGAGTACCTGAAGCCACTGGCGGAACGCTGGTTAGtggtgg                                                >  1:3413644/1‑69 (MQ=255)
                           aTGTAATTAATCGTCTGGTCGAGTACCTGAAGCCACTGGCGGAACGCTGGTTAGTGGTGGGTGACAAAt                                       <  1:3417625/69‑1 (MQ=255)
                           aTGTAATTAATCGTCTGGGCGAGTACCTGAAGCCACTGGCGGAACGCTGGTTAGTGGTGGGTGACAAAt                                       <  1:2635744/69‑1 (MQ=255)
                           aTGTAATTAATCGTCTGGGCGAGTACCTGAAGCCACTGGCGGAACGCTGGTTAGTGGTGGGTGACAAAt                                       <  1:1568136/69‑1 (MQ=255)
                           aTGTAATTAATCGTCTGGGCGAGTACCTGAAGCCACTGGCGGAACGCTGGTTAGTGGTGGGTGACAAAt                                       <  1:2479502/69‑1 (MQ=255)
                           aTGTAATTAATCGTCTGGGCGAGTACCTGAAGCCACTGGCGGAACGCTGGTTAGTGGTGGGTGACAAAt                                       <  1:2903540/69‑1 (MQ=255)
                           aTGTAATTAATCGTCTGGGCGAGTACCTGAAGCCACTGGCGGAACGCTGGTTAGTGGTGGGTGACAAAt                                       <  1:774212/69‑1 (MQ=255)
                           aTGTAATTAATCGTCTGGGCGAGTACCTGAAGCCACTGGCGGAACGCTGGTTAGTGGTGGGTGACAAAt                                       <  1:807632/69‑1 (MQ=255)
                             gtgATTAATCGTCTGGGCGAATACGTGAAGCCGCTGGCAGAACGCTGGTTAGTGGTGGGTGACAAAttt                                     >  1:3295486/1‑69 (MQ=255)
                             gtgATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAgt                                                     <  1:1063984/53‑1 (MQ=255)
                             gtgATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAgt                                                     <  1:1826025/53‑1 (MQ=255)
                             gtgATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAgt                                                     <  1:1832507/53‑1 (MQ=255)
                             gtgATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAGTGGTGGGTGACAAAttt                                     >  1:3272618/1‑69 (MQ=255)
                             gtgATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAGTGGTGGGTGACAAAttt                                     >  1:3304379/1‑69 (MQ=255)
                             gtgATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAGTGGTGGGTGACAAAttt                                     >  1:2860437/1‑69 (MQ=255)
                             gtgATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAGTGGTGGGTGACAAAttt                                     >  1:1668464/1‑69 (MQ=255)
                             gtgATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAGTGGTGGGTGACAAAttt                                     >  1:2591944/1‑69 (MQ=255)
                             gtgATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAGTGGTGGGTGACAAAttt                                     >  1:755993/1‑69 (MQ=255)
                             gtgATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAGTGGTGGGTGACAAAttt                                     >  1:2894449/1‑69 (MQ=255)
                             gtgATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAGTGGTGGGTGACAAAtt                                      >  1:1079781/1‑68 (MQ=255)
                                aTTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAGTGGTGGGTGACAAATTTGt                                   >  1:1736648/1‑68 (MQ=255)
                                     tCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAGTGGTGGGTGACAAATTTGTTTTAgg                             >  1:899239/1‑69 (MQ=255)
                                     tCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAGTGGTGGGTGACAAATTTGTTTTAgg                             >  1:3160027/1‑69 (MQ=255)
                                     tCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAGTGGTGGGTGACAAATTTGTTTTAgg                             >  1:3184474/1‑69 (MQ=255)
                                     tCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAGTGGTGGGTGACAAATTTGTTTTAgg                             >  1:603561/1‑69 (MQ=255)
                                     tCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAGTGGTGGGTGACAAATTTGTTTTAgg                             >  1:640454/1‑69 (MQ=255)
                                     tCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAGTGGTGGGTGACAAATTTGTTTTAgg                             >  1:89491/1‑69 (MQ=255)
                                       gTCTGGGCGAGTACCTGAAGCCACTGGCGGAACGCTGGTTAGTGGTGGGTGACAAATTTGTTTTAGGt                            >  1:3369345/1‑68 (MQ=255)
                                       gTCTGGGCGAGTACCTGAAGCCACTGGCGGAACGCTGGTTAGTGGTGGGTGACAAATTTGTTTTAGGt                            >  1:630986/1‑68 (MQ=255)
                                       gTCTGGGCGAGTACCTGAAGCCACTGGCGGAACGCTGGTTAGTGGTGGGTGACAAATTTGTTTTAGGt                            >  1:2578488/1‑68 (MQ=255)
                                       gTCTGGGCGAGTACCTGAAGCCACTGGCGGAACGCTGGTTAGTGGTGGGTGACAAATTTGTTTTAGGt                            >  1:1353236/1‑68 (MQ=255)
                                       gTCTGGGCGAGTACCTGAAGCCACTGGCGGAACGCTGGTTAGTGGTGGGTGACAAATTTGTTTTAGGt                            >  1:1430142/1‑68 (MQ=255)
                                       gTCTGGGCGAGTACCTGAAGCCACTGGCGGAACGCTGGTTAGTGGTGGGTGACAAATTTGTTTTAGGt                            >  1:1724767/1‑68 (MQ=255)
                                       gTCTGGGCGAGTACCTGAAGCCACTGGCGGAACGCTGGTTAGTGGTGGGTGACAAATTTGTTTTAGGt                            >  1:2536852/1‑68 (MQ=255)
                                       gTCTGGGCGAGTACCTGAAGCCACTGGCGGAACGCTGGTTAGTGGTGGGTGACAAATTTGTTTTAGGt                            >  1:2503040/1‑68 (MQ=255)
                                       gTCTGGGCGAGTACCTGAAGCCACTGGCGGAACGCTGGTTAGTGGTGGGTGACAAATTTGTTTTAGGt                            >  1:2240197/1‑68 (MQ=255)
                                       gTCTGGGCGAGTACCTGAAGCCACTGGCGGAACGCTGGTTAGTGGTGGGTGACAAATTTGTTTTAGGt                            >  1:1743801/1‑68 (MQ=255)
                                       gTCTGGGCGAGTACCTGAAGCCACTGGCGGAACGCTGGTTAGTGGTGGGTGACAAATTTGTTTTAGGt                            >  1:1749275/1‑68 (MQ=255)
                                       gTCTGGGCGAGTACCTGAAGCCACTGGCGGAACGCTGGTTAGTGGTGGGTGACAAATTTGTTTTAGGt                            >  1:2190152/1‑68 (MQ=255)
                                       gTCTGGGCGAGTACCTGAAGCCACTGGCGGAACGCTGGTTAGTGGTGGGTGACAAATTTGTTTTAGGt                            >  1:1858807/1‑68 (MQ=255)
                                       gTCTGGGCGAGTACCTGAAGCCACTGGCGGAACGCTGGTTAGTGGTGGGTGACAAATTTGTTTTAGGt                            >  1:2053492/1‑68 (MQ=255)
                                       gTCTGGGCGAGTACCTGAAGCCACTGGCGGAACGCTGGTTAGTGGTGGGTGACAAATTTGTTTTAGGt                            >  1:2094912/1‑68 (MQ=255)
                                                 aTACCTGAAGCCGCTGGCAGAACGCTGGTTAGTGGTGGGTGACAAATTTGTTTTAGGTTTTGCTCAATc                 <  1:3340777/69‑1 (MQ=255)
                                                  tACCTGAAGCCGCTGGCAGAACGCTGGTTAGTGGTGGGTGACAAATTTGTTTTAGGTTTTGCTCAATcc                >  1:2806935/1‑69 (MQ=255)
                                                  tACCTGAAGCCGCTGGCAGAACGCTGGTTAGTGGTGGGTGACAAATTTGTTTTAGGTTTTGCTCAATcc                >  1:2765592/1‑69 (MQ=255)
                                                          gCCACTGGCGGAACGCTGGTTAGTGGTGGGTGACAAATTTGTTTTAGGTTTTGCTCAATCCACTGTc          <  1:1021912/67‑1 (MQ=255)
                                                          gCCACTGGCGGAACGCTGGTTAGTGGTGGGTGACAAATTTGTTTTAGGTTTTGCTCAATCCACTGTc          <  1:1504679/67‑1 (MQ=255)
                                                          gCCACTGGCGGAACGCTGGTTAGTGGTGGGTGACAAATTTGTTTTAGGTTTTGCTCAATCCACTGTc          <  1:6207/67‑1 (MQ=255)
                                                          gCCACTGGCGGAACGCTGGTTAGTGGTGGGTGACAAATTTGTTTTAGGTTTTGCTCAATCCACTGTc          <  1:485974/67‑1 (MQ=255)
                                                          gCCACTGGCGGAACGCTGGTTAGTGGTGGGTGACAAATTTGTTTTAGGTTTTGCTCAATCCACTGTc          <  1:1441098/67‑1 (MQ=255)
                                                               tGGCGGAACGCTGGTTAGTGGTGGGTGACAAATTTGTTTTAGGTTTTGCTCAATCCACTGTCgaga      >  1:199857/1‑66 (MQ=255)
                                                               tGGCGGAACGCTGGTTAGTGGTGGGTGACAAATTTGTTTTAGGTTTTGCTCAATCCACTGTCGAGaa     >  1:1315020/1‑67 (MQ=255)
                                                               tGGCGGAACGCTGGTTAGTGGTGGGTGACAAATTTGTTTTAGGTTTTGCTCAATCCACTGTCGAGaa     >  1:2807447/1‑67 (MQ=255)
                                                               tGGCGGAACGCTGGTTAGTGGTGGGTGACAAATTTGTTTTAGGTTTTGCTCAATCCACTGTCGAGaa     >  1:1405177/1‑67 (MQ=255)
                                                               tGGCGGAACGCTGGTTAGTGGTGGGTGACAAATTTGTTTTAGGTTTTGCTCAATCCACTGTCGAGaa     >  1:142417/1‑67 (MQ=255)
                                                               tGGCGGAACGCTGGTTAGTGGTGGGTGACAAATTTGTTTTAGGTTTTGCTCAATCCACTGTCGAGaa     >  1:1314335/1‑67 (MQ=255)
                                                               tGGCGGAACGCTGGTTAGTGGTGGGTGACAAATTTGTTTTAGGTTTTGCTCAATCCACTGTCGAGaa     >  1:1489820/1‑67 (MQ=255)
                                                               tGGCGGAACGCTGGTTAGTGGTGGGTGACAAATTTGTTTTAGGTTTTGCTCAATCCACTGTCGAGaa     >  1:880117/1‑67 (MQ=255)
                                                               tGGCGGAACGCTGGTTAGTGGTGGGTGACAAATTTGTTTTAGGTTTTGCTCAATCCACTGTCGAGaa     >  1:1077626/1‑67 (MQ=255)
                                                               tGGCGGAACGCTGGTTAGTGGTGGGTGACAAATTTGTTTTAGGTTTTGCTCAATCCACTGTCGAGaa     >  1:606967/1‑67 (MQ=255)
                                                               tGGCGGAACGCTGGTTAGTGGTGGGTGACAAATTTGTTTTAGGTTTTGCTCAATCCACTGTCGAGaa     >  1:2561772/1‑67 (MQ=255)
                                                               tGGCGGAACGCTGGTTAGTGGTGGGTGACAAATTTGTTTTAGGTTTTGCTCAATCCACTGTCGAGaa     >  1:2981678/1‑67 (MQ=255)
                                                               tGGCGGAACGCTGGTTAGTGGTGGGTGACAAATTTGTTTTAGGTTTTGCTCAATCCACTGTCGAGaa     >  1:2990083/1‑67 (MQ=255)
                                                               tGGCGGAACGCTGGTTAGTGGTGGGTGACAAATTTGTTTTAGGTTTTGCTCAATCCACTGTCGAGaa     >  1:1763970/1‑67 (MQ=255)
                                                               tGGCGGAACGCTGGTTAGTGGTGGGTGACAAATTTGTTTTAGGTTTTGCTCAATCCACTGTCGAGaa     >  1:1813786/1‑67 (MQ=255)
                                                               tGGCGGAACGCTGGTTAGTGGTGGGTGACAAATTTGTTTTAGGTTTTGCTCAATCCACTGTCGAGaa     >  1:203408/1‑67 (MQ=255)
                                                               tGGCGGAACGCTGGTTAGTGGTGGGTGACAAATTTGTTTTAGGTTTTGCTCAATCCACTGTCGAGaa     >  1:309149/1‑67 (MQ=255)
                                                               tGGCGGAACGCTGGTTAGTGGTGGGTGACAAATTTGTTTTAGGTTTTGCTCAATCCACTGTCGAGaa     >  1:2070748/1‑67 (MQ=255)
                                                               tGGCGGAACGCTGGTTAGTGGTGGGTGACAAATTTGTTTTAGGTTTTGCTCAATCCACTGTCGAGaa     >  1:3268777/1‑67 (MQ=255)
                                                               tGGCGGAACGCTGGTTAGTGGTGGGTGACAAAATTGATTTAGGTTTTGCTCAATCCACTGTCGAGaa     >  1:2180578/1‑67 (MQ=255)
                                                               aggCGGAACGCTGGTTAGTGGTGGGTGACAAATTTGTTTTAGGTTTTGCTCAATCCACTGTCGAGaa     >  1:1892267/2‑67 (MQ=255)
                                                                ggCAGAACGCTGGTTAGTGGTGGGTGACAAATTTGTTTTAGGTTTTGTTCAATCCACTGTCGAGAAAAg  <  1:2156482/69‑1 (MQ=255)
                                                                ggCAGAACGCTGGTTAGTGGTGGGTGACAAATTTGTTTTAGGTTTTGCTCAATCCACTGTCGAGAAAAg  <  1:451350/69‑1 (MQ=255)
                                                                ggCAGAACGCTGGTTAGTGGTGGGTGACAAATTTGTTTTAGGTTTTGCTCAATCCACTGTCGAGAAAAg  <  1:3024293/69‑1 (MQ=255)
                                                                   |                                                                 
CACCGGGTAAATACATCCAGGGCGCTGATGTGATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAGTGGTGGGTGACAAATTTGTTTTAGGTTTTGCTCAATCCACTGTCGAGAAAAG  >  minE/2292889‑2293021

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A