Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,332,248 |
G→C |
32.2% |
V37V (GTC→GTG) |
fdhD ← |
formate dehydrogenase formation protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,332,248 | 0 | G | C | 32.2%
| 122.6
/ 82.8
| 112 | V37V (GTC→GTG) | fdhD | formate dehydrogenase formation protein |
| Reads supporting (aligned to +/- strand): ref base G (39/36); new base C (15/21); total (54/58) |
| Fisher's exact test for biased strand distribution p-value = 3.20e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01 |
Only 100 of 120 total aligned reads displayed.
GGCGACGCCATCATCACCACATGCGAAATGCCGTTGTAGACCAGCGCAACGGGAACTTCTTCCGCGACCTCATCTAACCGTGGGTGTTGTAAATCATCACGCCGCCATAACTCAATTTGACG > minE/2332183‑2332304
|
ggCGACGCCATCATCACCACATGCGAAATGCCGTTGTAGACCAGCGCAACGGGAACTTCTTCcgcg < 1:1055802/66‑1 (MQ=255)
ggCGACGCCATCATCACCACATGCGAAATGCCGTTGTAGACCAGCGCAACGGGAACTTCTTCcgcg < 1:3203175/66‑1 (MQ=255)
ggCGACGCCATCATCACCACATGCGAAATGCCGTTGTAGACCAGCGCAACGGGAACTTCTTCcgcg < 1:3175849/66‑1 (MQ=255)
ggCGACGCCATCATCACCACATGCGAAATGCCGTTGTAGACCAGCGCAACGGGAACTTCTTCcgcg < 1:3104880/66‑1 (MQ=255)
ggCGACGCCATCATCACCACATGCGAAATGCCGTTGTAGACCAGCGCAACGGGAACTTCTTCcgcg < 1:1327199/66‑1 (MQ=255)
ggCGACGCCATCATCACCACATGCGAAATGCCGTTGTAGACCAGCGCAACGGGAACTTCTTCcgcg < 1:2881504/66‑1 (MQ=255)
ggCGACGCCATCATCACCACATGCGAAATGCCGTTGTAGACCAGCGCAACGGGAACTTCTTCcgcg < 1:17821/66‑1 (MQ=255)
ggCGACGCCATCATCACCACATGCGAAATGCCGTTGTAGACCAGCGCAACGGGAACTTCTTCcgcg < 1:1725919/66‑1 (MQ=255)
gCCATCATCACCACATGCGAAATGCCGTTGTAGACCAGCGCAACGGGAACTTCTTCCGCGACCTCa < 1:973999/66‑1 (MQ=255)
gCCATCATCACCACATGCGAAATGCCGTTGTAGACCAGCGCAACGGGAACTTCTTCCGCGACCTCa < 1:949410/66‑1 (MQ=255)
gCCATCATCACCACATGCGAAATGCCGTTGTAGACCAGCGCAACGGGAACTTCTTCCGCGACCTCa < 1:2615626/66‑1 (MQ=255)
gCCATCATCACCACATGCGAAATGCCGTTGTAGACCAGCGCAACGGGAACTTCTTCCGCGACCTCa < 1:216591/66‑1 (MQ=255)
gCCATCATCACCACATGCGAAATGCCGTTGTAGACCAGCGCAACGGGAACTTCTTCCGCGACCTCa < 1:1847524/66‑1 (MQ=255)
gCCATCATCACCACATGCGAAATGCCGTTGTAGACCAGCGCAACGGGAACTTCTTCCGCGACCTCa < 1:1734413/66‑1 (MQ=255)
gCCATCATCACCACATGCGAAATGCCGTTGTAGACCAGCGCAACGGGAACTTCTTCCGCGACCTCATCt > 1:977555/1‑69 (MQ=255)
gCCATCATCACCACATGCGAAATGCCGTTGTAGACCAGCGCAACGGGAACTTCTTCCGCGACCTCATCt > 1:777597/1‑69 (MQ=255)
gCCATCATCACCACATGCGAAATGCCGTTGTAGACCAGCGCAACGGGAACTTCTTCCGCGACCTCATCt > 1:1078747/1‑69 (MQ=255)
ccATCATCACCACATGCGAAATGCCGTTGTAGACCAGCGCAACGGGAACTTCTTCCGCGACCTCATCTa < 1:3035243/69‑1 (MQ=255)
ccATCATCACCACATGCGAAATGCCGTTGTAGACCAGCGCAACGGGAACTTCTTCCGCGACCTCATCTa < 1:391185/69‑1 (MQ=255)
ccATCATCACCACATGCGAAATGCCGTTGTAGACCAGCGCAACGGGAACTTCTTCCGCGACCTCATCTa < 1:478871/69‑1 (MQ=255)
catcaCCACATGCGAAATGCCGTTGTAGACCAGCGCAACGGGAACTTCTTCCGCGACCTCATCTAACCg > 1:710252/1‑69 (MQ=255)
atcaCCACATGCGAAATGCCGTTGTAGACCAGCGCAACGGGAACTTCTTCCGCGACCTCATCTAACCGt > 1:3022054/1‑69 (MQ=255)
atcaCCACATGCGAAATGCCGTTGTAGACCAGCGCAACGGGAACTTCTTCCGCGACCTCATCTAACCGt > 1:610417/1‑69 (MQ=255)
aaTGCCGTTGTAGACCAGCGCAACGGGAACTTCTTCCGCGACCTCATCTAACCGTGGGTGTTGTAAatc < 1:974873/69‑1 (MQ=255)
aaTGCCGTTGTAGACCAGCGCAACGGGAACTTCTTCCGCGACCTCATCTAACCGTGGGTGTTGTAAatc < 1:2850164/69‑1 (MQ=255)
aaTGCCGTTGTAGACCAGCGCAACGGGAACTTCTTCCGCGACCTCATCTAACCGTGGGTGTTGTAAatc < 1:385291/69‑1 (MQ=255)
tGCCGTTGTAGACCAGCGCAACGGGAACTTCTTCCGCGACCTCATCTAACCGTGGGTGTTGTAAatcat < 1:3287809/69‑1 (MQ=255)
tGCCGTTGTAGACCAGCGCAACGGGAACTTCTTCCGCGACCTCATCTAACCGTGGGTGTTGTAAatcat < 1:808653/69‑1 (MQ=255)
tGCCGTTGTAGACCAGCGCAACGGGAACTTCTTCCGCGACCTCATCTAACCGTGGGTGTTGTAAatcat < 1:680257/69‑1 (MQ=255)
ttGTAGACCAGCGCAACGGGAACTTCTTCGGCCACCTCATCTAACCGTGGGTGTTGTaa < 1:539990/59‑1 (MQ=255)
ttGTAGACCAGCGCAACGGGAACTTCTTCCGCCACCTCATCTAACCGTGGGTGTTGTaa < 1:3134569/59‑1 (MQ=255)
ttGTAGACCAGCGCAACGGGAACTTCTTCCGCCACCTCATCTAACCGTGGGTGTTGTaa < 1:3191305/59‑1 (MQ=255)
ttGTAGACCAGCGCAACGGGAACTTCTTCCGCCACCTCATCTAACCGTGGGTGTTGTaa < 1:2268757/59‑1 (MQ=255)
ttGTAGACCAGCGCAACGGGAACTTCTTCCGCCACCTCATCTAACCGTGGGTGTTGTaa < 1:1006601/59‑1 (MQ=255)
ttGTAGACCAGCGCAACGGGAACTTCTTCCGCCACCTCATCTAACCGTGGGTGTTGTaa < 1:3098506/59‑1 (MQ=255)
ttGTAGACCAGCGCAACGGGAACTTCTTCCGCCACCTCATCTAACCGTGGGTGTTGTaa < 1:2967941/59‑1 (MQ=255)
ttGTAGACCAGCGCAACGGGAACTTCTTCCGCCACCTCATCTAACCGTGGGTGTTGTaa < 1:2658574/59‑1 (MQ=255)
ttGTAGACCAGCGCAACGGGAACTTCTTCCGCCACCTCATCTAACCGTGGGTGTTGTaa < 1:2632941/59‑1 (MQ=255)
ttGTAGACCAGCGCAACGGGAACTTCTTCCGCCACCTCATCTAACCGTGGGTGTTGTaa < 1:225588/59‑1 (MQ=255)
ttGTAGACCAGCGCAACGGGAACTTCTTCCGCCACCTCATCTAACCGTGGGTGTTGTaa < 1:705871/59‑1 (MQ=255)
ttGTAGACCAGCGCAACGGGAACTTCTTCCGCCACCTCATCTAACCGTGGGTGTTGTaa < 1:771072/59‑1 (MQ=255)
ttGTAGACCAGCGCAACGGGAACTTCTTCCGCCACCTCATCTAACCGTGGGTGTTGTaa < 1:1983173/59‑1 (MQ=255)
ttGTAGACCAGCGCAACGGGAACTTCTTCCGCCACCTCATCTAACCGTGGGTGTTGTaa < 1:811280/59‑1 (MQ=255)
ttGTAGACCAGCGCAACGGGAACTTCTTCCGCCACCTCATCTAACCGTGGGTGTTGTaa < 1:1888332/59‑1 (MQ=255)
ttGTAGACCAGCGCAACGGGAACTTCTTCCGCCACCTCATCTAACCGTGGGTGTTGTaa < 1:1884677/59‑1 (MQ=255)
ttGTAGACCAGCGCAACGGGAACTTCTTCCGCCACCTCATCTAACCGTGGGTGTTGTaa < 1:1870171/59‑1 (MQ=255)
ttGTAGACCAGCGCAACGGGAACTTCTTCCGCCACCTCATCTAACCGTGGGTGTTGTaa < 1:1560161/59‑1 (MQ=255)
gTAGACCAGCGCAACGGGAACTTCTTCCGCGACCTCATCTAACCGTGGGTGTTGTAAatc < 1:1316242/60‑1 (MQ=255)
gTAGACCAGCGCAACGGGAACTTCTTCCGCGACCTCATCTAACCGTGGGTGTTGTAAatc < 1:1696003/60‑1 (MQ=255)
gTAGACCAGCGCAACGGGAACTTCTTCCGCGACCTCATCTAACCGTGGGTGTTGTAAatc < 1:136695/60‑1 (MQ=255)
gTAGACCAGCGCAACGGGAACTTCTTCCGCGACCTCATCTAACCGTGGGTGTTGTAAatc < 1:1387385/60‑1 (MQ=255)
gTAGACCAGCGCAACGGGAACTTCTTCCGCGACCTCATCTAACCGTGGGTGTTGTAAatc < 1:2559635/60‑1 (MQ=255)
gTAGACCAGCGCAACGGGAACTTCTTCCGCGACCTCATCTAACCGTGGGTGTTGTAAatc < 1:2255143/60‑1 (MQ=255)
gTAGACCAGCGCAACGGGAACTTCTTCCGCGACCTCATCTAACCGTGGGTGTTGTAAatc < 1:1985545/60‑1 (MQ=255)
gTAGACCAGCGCAACGGGAACTTCTTCCGCGACCTCATCTAACCGTGGGTGTTGTAAATCATCAcgcc > 1:2517550/1‑68 (MQ=255)
gTAGACCAGCGCAACGGGAACTTCTTCCGCGACCTCATCTAACCGTGGGTGTTGTAAATCATCAcgcc > 1:3017409/1‑68 (MQ=255)
gTAGACCAGCGCAACGGGAACTTCTTCCGCGACCTCATCTAACCGTGGGTGTTGTAAATCATCAcgcc > 1:812522/1‑68 (MQ=255)
gTAGACCAGCGCAACGGGAACTTCTTCCGCAACCTCATCTAACCGTGGGTGTTGTAAatc < 1:2008070/60‑1 (MQ=255)
tAGACCAGCGCAACGGGAACTTCTTCCGCGACCTCATCTAACCGTGGGTGTTGTAAatc < 1:2613881/59‑1 (MQ=255)
aCCAGCGCAACGGGAACTTCTTCCGCCACCTCATCTAACCGTGGGTGTTGTAAATCATCACGCCGCCa > 1:1949827/1‑68 (MQ=255)
aCCAGCGCAACGGGAACTTCTTCCGCCACCTCATCTAACCGTGGGTGTTGTAAATCATCACGCCGCCAt > 1:464693/1‑69 (MQ=255)
aCCAGCGCAACGGGAACTTCTTCCGCCACCTCATCTAACCGTGGGTGTTGTAAATCATCACGCCGCCAt > 1:512428/1‑69 (MQ=255)
aCCAGCGCAACGGGAACTTCTTCCGCCACCTCATCTAACCGTGGGTGTTGTAAATCATCACGCCGCCAt > 1:3428126/1‑69 (MQ=255)
aCCAGCGCAACGGGAACTTCTTCCGCCACCTCATCTAACCGTGGGTGTTGTAAATCATCACGCCGCCAt > 1:2265392/1‑69 (MQ=255)
aCCAGCGCAACGGGAACTTCTTCCGCCACCTCATCTAACCGTGGGTGTTGTAAATCATCACGCCGCCAt > 1:721774/1‑69 (MQ=255)
aCCAGCGCAACGGGAACTTCTTCCGCCACCTCATCTAACCGTGGGTGTTGTAAATCATCACGCCGCCAt > 1:3169403/1‑69 (MQ=255)
aCCAGCGCAACGGGAACTTCTTCCGCCACCTCATCTAACCGTGGGTGTTGTAAATCATCACGCCGCCAt > 1:2372876/1‑69 (MQ=255)
aCCAGCGCAACGGGAACTTCTTCCGCCACCTCATCTAACCGTGGGTGTTGTAAATCATCACGCCGCCAt > 1:922837/1‑69 (MQ=255)
aCCAGCGCAACGGGAACTTCTTCCGCCACCTCATCTAACCGTGGGTGTTGTAAATCATCACGCCGCCAt > 1:1077253/1‑69 (MQ=255)
aCCAGCGCAACGGGAACTTCTTCCGCCACCTCATCTAACCGTGGGTGTTGTAAATCATCACGCCGCCAt > 1:2664817/1‑69 (MQ=255)
ccAGCGCAACGGGAACTTCTTCCGCGACCTCATCTAACCGTGGGTGTTGTAAatcat < 1:2429751/57‑1 (MQ=255)
cAGCGCAACGGGAACTTCTTCCGCGACCTCATCTAACCGTg > 1:2114564/1‑41 (MQ=255)
cAGCGCAACGGGAACTTCTTCCGCGACCTCATCTAACCGTGGGTGTTGTAAatcatc > 1:2508973/1‑57 (MQ=255)
cAGCGCAACGGGAACTTCTTCCGCGACCTCATCTAACCGTGGGTGTTGTAAATCATCACGCCGCCATaa > 1:1099102/1‑69 (MQ=255)
cAGCGCAACGGGAACTTCTTCCGCGACCTCATCTAACCGTGGGTGTTGTAAATCATCACGCCGCCATaa > 1:1531231/1‑69 (MQ=255)
cAGCGCAACGGGAACTTCTTCCGCGACCTCATCTAACCGTGGGTGTTGTAAATCATCACGCCGCCATaa > 1:689395/1‑69 (MQ=255)
cAGCGCAACGGGAACTTCTTCCGCGACCTCATCTAACCGTGGGTGTTGTAAATCATCACGCCGCCATaa > 1:155075/1‑69 (MQ=255)
cAGCGCAACGGGAACTTCTTCCGCGACCTCATCTAACCGTGGGTGTTGTAAATCATCACGCCGCCATaa > 1:2058070/1‑69 (MQ=255)
cAGCGCAACGGGAACTTCTTCCGCGACCTCATCTAACCGTGGGTGTTGTAAATCATCACGCCGCCATaa > 1:1655956/1‑69 (MQ=255)
cAGCGCAACGGGAACTTCTTCCGCGACCTCATCTAACCGTGGGTGTTGTAAATCATCACGCCGCCATaa > 1:2561000/1‑69 (MQ=255)
cAGCGCAACGGGAACTTCTTCCGCGACCTCATCTAACCGTGGGTGTTGTAAATCATCACGCCGCCATaa > 1:2430316/1‑69 (MQ=255)
cAGCGCAACGGGAACTTCTTCCGCGACCTCATCTAACCGTGGGTGTTGTAAATCATCACGCCGCCATaa > 1:362271/1‑69 (MQ=255)
cAGCGCAACGGGAACTTCTTCCGCGACCTCATCTAACCGTGGGTGTTGTAAATCATCACGCCGCCATaa > 1:2334305/1‑69 (MQ=255)
cAGCGCAACGGGAACTTCTTCCGCGACCTCATCTAACCGTGGGTGTTGTAAATCATCACGCCGCCATaa > 1:3286630/1‑69 (MQ=255)
cAGCGCAACGGGAACTTCTTCCGCGACCTCATCTAACCGTGGGTGTTGTAAATCATCACGCCGCCATaa > 1:3180180/1‑69 (MQ=255)
cAGCGCAACGGGAACTTCTTCCGCGACCTCATCTAACCGTGGGTGTTGTAAATCATCACGCCGCCATaa > 1:2932071/1‑69 (MQ=255)
aCGGGAACTTCTTCCGCGACCTCATCTAACCGTGGGTGTTGTAAATCATCACGCCGCCATAACTCAAt > 1:2478045/1‑68 (MQ=255)
aCGGGAACTTCTTCCGCGACCTCATCTAACCGTGGGTGTTGTAAATCATCACGCCGCCATAACTCAAt > 1:3025025/1‑68 (MQ=255)
aCGGGAACTTCTTCCGCGACCTCATCTAACCGTGGGTGTTGTAAATCATCACGCCGCCATAACTCAAt > 1:2360431/1‑68 (MQ=255)
aCGGGAACTTCTTCCGCGACCTCATCTAACCGTGGGTGTTGTAAATCATCACGCCGCCATAACTCAAt > 1:3420725/1‑68 (MQ=255)
aCGGGAACTTCTTCCGCGACCTCATCTAACCGTGGGTGTTGTAAATCATCACGCCGCCATAACTCAAt > 1:2865729/1‑68 (MQ=255)
aCGGGAACTTCTTCCGCGACCTCATCTAACCGTGGGTGTTGTAAATCATCACGCCGCCATAACTCAAt > 1:2117808/1‑68 (MQ=255)
aaCTTCTTCCGCGACCTCATCTAACCGTGGGTGTTGTAAATCATCACGCCGCCATAACTCAATTTGACg < 1:1170112/69‑1 (MQ=255)
aaCTTCTTCCGCGACCTCATCTAACCGTGGGTGTTGTAAATCATCACGCCGCCATAACTCAATTTGACg < 1:1311749/69‑1 (MQ=255)
aaCTTCTTCCGCGACCTCATCTAACCGTGGGTGTTGTAAATCATCACGCCGCCATAACTCAATTTGACg < 1:1976033/69‑1 (MQ=255)
aaCTTCTTCCGCGACCTCATCTAACCGTGGGTGTTGTAAATCATCACGCCGCCATAACTCAATTTGACg < 1:2098863/69‑1 (MQ=255)
cttcttCCGCGACCTCATCTAACCGTGGGTGTTGTAAatcat > 1:279852/1‑42 (MQ=255)
cttcttCCGCGACCTCATCTAACCGTGGGTGTTGTAAatcat > 1:2004453/1‑42 (MQ=255)
cttcttCCGCGACCTCATCTAACCGTGGGTGTTGTAAatcat > 1:1413409/1‑42 (MQ=255)
cttcttCCGCGACCTCATCTAACCGTGGGTGTTGTAAatcat > 1:2114733/1‑42 (MQ=255)
|
GGCGACGCCATCATCACCACATGCGAAATGCCGTTGTAGACCAGCGCAACGGGAACTTCTTCCGCGACCTCATCTAACCGTGGGTGTTGTAAATCATCACGCCGCCATAACTCAATTTGACG > minE/2332183‑2332304
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
