Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,344,243 |
T→C |
27.6% |
V303V (GTT→GTC) |
glnG → |
fused DNA‑binding response regulator in two‑component regulatory system with GlnL, nitrogen regulator I (NRI) |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,344,243 | 0 | T | C | 27.6%
| 86.3
/ 34.3
| 83 | V303V (GTT→GTC) | glnG | fused DNA‑binding response regulator in two‑component regulatory system with GlnL, nitrogen regulator I (NRI) |
| Reads supporting (aligned to +/- strand): ref base T (26/34); new base C (20/3); total (46/37) |
| Fisher's exact test for biased strand distribution p-value = 4.14e-04 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CACCAGAATCTCGAACAGCGAGTGCAGGAAGGTAAGTTCCGTGAGGATCTGTTCCACCGCCTGAACGTTATCCGCGTTCATCTGCCGCCGCTGCGCGAACGTCGGGAAGATATTCCCCGTCTGGCGCGCCATTTTT > minE/2344175‑2344310
|
cACCAGAATCTCGAACGGCGAGTGCAGGAAGGTAAGTTCCGTGAGGATCTGTTCCACCGCCTGAACGtt < 1:579467/69‑1 (MQ=255)
cACCAGAATCTCGAACAGCGAGTGCAGGAAGGTAAGTTCCGTGAGGATCTGTTCCACCGCCTGAACGtt < 1:1357338/69‑1 (MQ=255)
cACCAGAATCTCGAACAGCGAGTGCAGGAAGGTAAGTTCCGTGAGGATCTGTTCCACCGCCTGAACGtt < 1:1779072/69‑1 (MQ=255)
cACCAGAATCTCGAACAGCGAGTGCAGGAAGGTAAGTTCCGTGAGGATCTGTTCCACCGCCTGAACGtt < 1:1940778/69‑1 (MQ=255)
cACCAGAATCTCGAACAGCGAGTGCAGGAAGGTAAGTTCCGTGAGGATCTGTTCCACCGCCTGAACGtt < 1:3136342/69‑1 (MQ=255)
cACCAGAATCTCGAACAGCGAGTGCAGGAAGGTAAGTTCCGTGAGGATCTGTTCCACCGCCTGAACGtt < 1:3072374/69‑1 (MQ=255)
cACCAGAATCTCGAACAGCGAGTGCAGGAAGGTAAGTTCCGTGAGGATCTGTTCCACCGCCTGAACGtt < 1:3021106/69‑1 (MQ=255)
cACCAGAATCTCGAACAGCGAGTGCAGGAAGGTAAGTTCCGTGAGGATCTGTTCCACCGCCTGAACGtt < 1:754533/69‑1 (MQ=255)
cACCAGAATCTCGAACAGCGAGTGCAGGAAGGTAAGTTCCGTGAGGATCTGTTCCACCGCCTGAACGtt < 1:2556845/69‑1 (MQ=255)
cACCAGAATCTCGAACAGCGAGTGCAGGAAGGTAAGTTCCGTGAGGATCTGTTCCACCGCCTGAACGtt < 1:2303021/69‑1 (MQ=255)
cACCAGAATCTCGAACAGCGAGTGCAGGAAGGTAAGTTCCGTGAGGATCTGTTCCACCGCCTGAACGtt < 1:2385674/69‑1 (MQ=255)
tccAGAATCTCGAACAGCGAGTGCACGAAGGTAAGTTCCGTGAGGATCTGTTCCACCGCCTGAACGtt < 1:1604848/67‑1 (MQ=255)
cAGAATCTCGAACAGCGAGTGCAGGAAGGTAAGTTCCGTGAGGATCTGTTCCACCGCCTGAACGTTAt > 1:1155683/1‑68 (MQ=255)
aGAATCTCGAACAGCGAGTGCAGGAAGGTAAGTTCCGTGAGGATCTGTTCCACCGCCTGAACGTTATcc > 1:2708352/1‑69 (MQ=255)
aGAATCTCGAACAGCGAGTGCAGGAAGGTAAGTTCCGTGAGGATCTGTTCCACCGCCTGAACGTTATcc > 1:2094469/1‑69 (MQ=255)
aGAATCTCGAACAGCGAGTGCAGGAAGGTAAGTTCCGTGAGGATCTGTTCCACCGCCTGAACGTTATcc > 1:1847375/1‑69 (MQ=255)
aGAATCTCGAACAGCGAGTGCAGGAAGGTAAGTTCCGTGAGGATCTGTTCCACCGCCTGAACGTTATcc > 1:296839/1‑69 (MQ=255)
aGAATCTCGAACAGCGAGTGCAGGAAGGTAAGTTCCGTGAGGATCTGTTCCACCGCCTGAACGTTATcc > 1:2279506/1‑69 (MQ=255)
aGAATCTCGAACAGCGAGTGCAGGAAGGTAAGTTCCGTGAGGATCTGTTCCACCGCCTGAACGTTATcc > 1:2469874/1‑69 (MQ=255)
aGAATCTCGAACAGCGAGTGCAGGAAGGTAAGTTCCGTGAGGATCTGTTCCACCGCCTGAACGTTATcc > 1:3355626/1‑69 (MQ=255)
aGAATCTCGAACAGCGAGTGCAGGAAGGTAAGTTCCGTGAGGATCTGTTCCACCGCCTGAACGTTATcc > 1:763080/1‑69 (MQ=255)
aGAATCTCGAACAGCGAGTGCAGGAAGGTAAGTTCCGTGAGGATCTGTTCCACCGCCTGAACGTTATcc > 1:1700842/1‑69 (MQ=255)
aGAATCTCGAACAGCGAGTGCAGGAAGGTAAGTTCCGTGAGGATCTGTTCCACCGCCTGAACGTTATcc > 1:1463296/1‑69 (MQ=255)
aGAATCTCGAACAGCGAGTGCAGGAAGGTAAGTTCCGTGAGGATCTGTTCCACAGCCTGAACGTTATcc > 1:1308780/1‑69 (MQ=255)
tcGAACAGCGAGTGCAGGAAGGTAAGTTCCGTGAGGATCTGTTCCACCGCCTGAACGTTATCCGCGTTc > 1:2016032/1‑69 (MQ=255)
aCAGCGAGTGCAGGAAGGTAAGTTCCGTGAGGATCTGTTCCACCGCCTGAACGTTATGCGCGTTc < 1:1502828/65‑1 (MQ=255)
aCAGCGAGTGCAGGAAGGTAAGTTCCGTGAGGATCTGTTCCACCGCCTGAACGTTATCCGCGTTc < 1:1225060/65‑1 (MQ=255)
aCAGCGAGTGCAGGAAGGTAAGTTCCGTGAGGATCTGTTCCACCGCCTGAACGTTATCCGCGTTc < 1:2131185/65‑1 (MQ=255)
aCAGCGAGTGCAGGAAGGTAAGTTCCGTGAGGATCTGTTCCACCGCCTGAACGTTATCCGCGTTc < 1:2107789/65‑1 (MQ=255)
aCAGCGAGTGCAGGAAGGTAAGTTCCGTGAGGATCTGTTCCACCGCCTGAACGTTATCCGCGTTc < 1:2757693/65‑1 (MQ=255)
aCAGCGAGTGCAGGAAGGTAAGTTCCGTGAGGATCTGTTCCACCGCCTGAACGTTATCCGCGTTc < 1:2762000/65‑1 (MQ=255)
aCAGCGAGTGCAGGAAGGTAAGTTCCGTGAGGATCTGTTCCACCGCCTGAACGTTATCCGCGTTc < 1:707830/65‑1 (MQ=255)
aCAGCGAGTGCAGGAAGGTAAGTTCCGTGAGGATCTGTTCCACCGCCTGAACGTTATCCGCGTTc < 1:1983062/65‑1 (MQ=255)
aCAGCGAGTGCAGGAAGGTAAGTTCCGTGAGGATCTGTTCCACCGCCTGAACGTTATCCGCGTTc < 1:1549420/65‑1 (MQ=255)
aCAGCGAGTGCAGGAAGGTAAGTTCCGTGAGGATCTGTTCCACCGCCTGAACGTTATCCGCGTTc < 1:1134264/65‑1 (MQ=255)
aCAGCGAGTGCAGGAAGGTAAGTTCCGTGAGGATCTGTTCCACCGCCTGAACGTTATCCGCGTTc < 1:1837680/65‑1 (MQ=255)
aCAGCGAGTGCAGGAAGGTAAGTTCCGTGAGGATCTGTTCCACCGCCTGAACGTTATCCGCGTTc < 1:1797259/65‑1 (MQ=255)
aCAGCGAGTGCAGGAAGGTAAGTTCCGTGAGGATCTGTTCCACCGCCTGAACGTTATCCGCGTTc < 1:2312601/65‑1 (MQ=255)
aCAGCGAGTGCAGGAAGGTAAGTTCAGTGAGGATCTGTTCCACCGCCTGAACGTTATCCGCGTTc < 1:258240/65‑1 (MQ=255)
aaGGTAAGTTCCGTGAGGATCTGTTCCACCGCCTGAACGTTATCCGCGTTCATCTGCCGCCGCTGcgcg < 1:964967/69‑1 (MQ=255)
aaGGTAAGTTCCGTGAGGATCTGTTCCACCGCCTGAACGTTATCCGCGTTCATCTGCCGCCGCTGcgcg < 1:2302967/69‑1 (MQ=255)
aaGGTAAGTTCCGTGAGGATCTGTTCCACCGCCTGAACGTTATCCGCGTTCATCTGCCGCCGCTGcgcg < 1:1061581/69‑1 (MQ=255)
tCCGTGAGGATCTGTTCCACCGCCTGAACGTCATCCGCGTTCATCTgccgc > 1:2564445/1‑51 (MQ=255)
tCCGTGAGGATCTGTTCCACCGCCTGAACGTCATCCGCGTTCATCTgccgc > 1:923219/1‑51 (MQ=255)
tCCGTGAGGATCTGTTCCACCGCCTGAACGTCATCCGCGTTCATCTgccgc > 1:249932/1‑51 (MQ=255)
tCCGTGAGGATCTGTTCCACCGCCTGAACGTCATCCGCGTTCATCTgccgc > 1:586705/1‑51 (MQ=255)
tCCGTGAGGATCTGTTCCACCGCCTGAACGTCATCCGCGTTCATCTgccgc > 1:3234180/1‑51 (MQ=255)
tCCGTGAGGATCTGTTCCACCGCCTGAACGTCATCCGCGTTCATCTgccgc > 1:1077966/1‑51 (MQ=255)
tCCGTGAGGATCTGTTCCACCGCCTGAACGTCATCCGCGTTCATCTgccgc > 1:394107/1‑51 (MQ=255)
tCCGTGAGGATCTGTTCCACCGCCTGAACGTCATCCGCGTTCATCTgccgc > 1:3372000/1‑51 (MQ=255)
tCCGTGAGGATCTGTTCCACCGCCTGAACGTCATCCGCGTTCATCTgccgc > 1:2294854/1‑51 (MQ=255)
tCCGTGAGGATCTGTTCCACCGCCTGAACGTCATCCGCGTTCATCTgccgc > 1:1782561/1‑51 (MQ=255)
tCCGTGAGGATCTGTTCCACCGCCTGAACGTCATCCGCGTTCATCTgccgc > 1:2279807/1‑51 (MQ=255)
tCCGTGAGGATCTGTTCCACCGCCTGAACGTCATCCGCGTTCATCTgccgc > 1:1896981/1‑51 (MQ=255)
tCCGTGAGGATCTGTTCCACCGCCTGAACGTCATCCGCGTTCATCTgccgc > 1:1963023/1‑51 (MQ=255)
tCCGTGAGGATCTGTTCCACCGCCTGAACGTCATCCGCGTTCATCTgccgc > 1:1162794/1‑51 (MQ=255)
tCCGTGAGGATCTGTTCCACCGCCTGAACGTCATCCGCGTTCATCTgccgc > 1:214668/1‑51 (MQ=255)
tCCGTGAGGATCTGTTCCACCGCCTGAACGTCATCCGCGTTCATCTgccgc > 1:2031105/1‑51 (MQ=255)
tCCGTGAGGATCTGTTCCACCGCCTGAACGTCATCCGCGTTCATCTgccg > 1:1531049/1‑50 (MQ=255)
tCCGTGAGGATCTGTTCCACCGCCTGAACGTCATACGCGTTCATCTgccgc > 1:1964474/1‑51 (MQ=38)
tCCGTGAGGATCTGTTCCACCGCCTGAACGTCAACCGCGt > 1:422028/1‑40 (MQ=37)
tCCGTGAGGATCTGTTACACCGCCTGAACGTCATCCGCGTTCATCTgccgc > 1:3425765/1‑51 (MQ=255)
tGAGGATCTGTTCCACCGCGTGAACGTTATCCGCGTTCATCTGCCGCCGCTGCGCGAACGTCGGGAAGa < 1:1644895/69‑1 (MQ=255)
tGAGGATCTGTTCCACCGCCTGAACGTTATCCGCGTTCATCTGCCGCCGCTGCGCGAACGTCGGGAAg < 1:840826/68‑1 (MQ=255)
tGAGGATCTGTTCCACCGCCTGAACGTTATCCGCGTTCATCTGCCGCCGCTGCGCGAACGTCGGGAAg < 1:93331/68‑1 (MQ=255)
tGAGGATCTGTTCCACCGCCTGAACGTTATCCGCGTTCATCTGCCGCCGCTGCGCGAACGTCGGGAAg < 1:1720093/68‑1 (MQ=255)
tGAGGATCTGTTCCACCGCCTGAACGTTATCCGCGTTCATCTGCCGCCGCTGCGCGAACGTCGGGAAg < 1:2030219/68‑1 (MQ=255)
tGAGGATCTGTTCCACCGCCTGAACGTTATCCGCGTTCATCTGCCGCCGCTGCGCGAACGTCGGGAAg < 1:148186/68‑1 (MQ=255)
tGAGGATCTGTTCCACCGCCTGAACGTTATCCGCGTTCATCTGCCGCCGCTGCGCGAACGTCGGGAAg < 1:1246293/68‑1 (MQ=255)
tGAGGATCTGTTCCACCGCCTGAACGTTATCCGCGTTCATCTGCCGCCGCTGCGCGAACGTCGGGAAg < 1:2235056/68‑1 (MQ=255)
tGAGGATCTGTTCCACCGCCTGAACGTTATCCGCGTTCATCTGCCGCCGCTGCGCGAACGTCGGGAAg < 1:3323845/68‑1 (MQ=255)
tGAGGATCTGTTCCACCGCCTGAACGTTATCCGCGTTCATCTGCCGCCGCTGCGCGAACGTCGGGAAg < 1:3328359/68‑1 (MQ=255)
tGAGGATCTGTTCCACCGCCTGAACGTTATCCGCGTTCATCTGCCGCCGCTGCGCGAACGTCGGGAAGa < 1:2435943/69‑1 (MQ=255)
tGAGGATCTGTTCCACCGCCTGAACGTTATCCGCGTTCATCTGCCGCCGCTGCGCGAACGTCGGGAAGa < 1:726482/69‑1 (MQ=255)
tGAGGATCTGTTCCACCGCCTGAACGTTATCCGCGTTCATCTGCCGCCGCTGCGCGAACGTCGGGAAGa < 1:2484888/69‑1 (MQ=255)
tGAGGATCTGTTCCACCGCCTGAACGTTATCCGCGTTCATCTGCCGCCGCTGCGCGAACGTCGGGAAGa < 1:3217208/69‑1 (MQ=255)
tGAGGATCTGTTCCACCGCCTGAACGTTATCCGCGTTCATCTGCCGCCGCTGCGCGAACGTCGGGAAGa < 1:27375/69‑1 (MQ=255)
tGAGGATCTGTTCCACCGCCTGAACGTTATCCGCGTTCATCTGCCGCCGCTGCGCGAACGTCGGGAAGa < 1:809444/69‑1 (MQ=255)
tGAGGATCTGTTCCACCGCCTGAACGTTATCCGCGTTCATCTGCCGCCGCTGCGCGAACGTCGGGAAGa < 1:2767793/69‑1 (MQ=255)
aTCTGTTCCACCGCCTGAACGTCATCCGCGTTCATCTGCCGCCACTGCGCGAACGTCGGGAAGATATTc < 1:2179620/69‑1 (MQ=255)
aTCTGTTCCACCGCCTGAACGTCATCCGCGTTCATCTGCCGCCACTGCGCGAACGTCGGGAAGATATTc < 1:666079/69‑1 (MQ=255)
aTCTGTTCCACCGCCTGAACGTCATCCGCGTTCATCTGCCGCCACTGCGCGAACGTCGGGAAGATATTc < 1:785700/69‑1 (MQ=255)
tCTGTTCCACCGCCTGAACGTTATCCGCGTTCATCTGCCGCCGCTGCGCGAACGTCGGGAAGATAttac > 1:3316893/1‑67 (MQ=255)
tCTGTTCCACCGCCTGAACGTTATCCGCGTTCATCTGCCGCCGCTGCGCGAACGTCGGGAAGATATTcc > 1:991048/1‑69 (MQ=255)
tCTGTTCCACCGCCTGAACGTTATCCGCGTTCATCTGCCGCCGCTGCGCGAACGTCGGGAAGATATTcc > 1:2875004/1‑69 (MQ=255)
tCTGTTCCACCGCCTGAACGTTATCCGCGTTCATCTGCCGCCGCTGCGCGAACGTCGGGAAGATATTcc > 1:2168429/1‑69 (MQ=255)
tCTGTTCAACCGCCTGAACGTTATCCGCGTTCATCTgccgcc > 1:932874/1‑42 (MQ=255)
gCCTGAACGTTATCCGCGTTCATCTGCCGCCGCTGcgcg > 1:2465199/1‑39 (MQ=255)
gCCTGAACGTTATCCGCGTTCATCTGCCGCCGCTGCGCGAACGTCGGGAAGATATTCCCCGTCTGgcgc > 1:3378410/1‑69 (MQ=255)
gCCTGAACGTTATCCGCGTTCATCTGCCGCCGCTGCGCGAACGTCGGGAAGATATTCCCCGTCTGgcgc > 1:3183695/1‑69 (MQ=255)
gCCTGAACGTTATCCGCGTTCATCTGCCGCCGCTGCGCGAACGTCGGGAAGATATTCCCCGTCTGgcgc > 1:2800538/1‑69 (MQ=255)
aaCGTTATCCGCGTTCATCTGCCGCCGCTGCGCGAACGTCGGGAAGatat > 1:129670/1‑50 (MQ=255)
aaCGTTATCCGCGTTCATCTGCCGCCGCTGCGCGAACGTCGGGAAGATATTCCCCGTCTGgcg > 1:2070338/1‑63 (MQ=255)
aaCGTTATCCGCGTTCATCTGCCGCCGCTGCGCGAACGTCGGGAAGATATTCCCCGTCTGGCGCGCCAt > 1:1744494/1‑69 (MQ=255)
aaCGTTATCCGCGTTCATCTGCCGCCGCTGCGCGAACGTCGGGAAGATATTCCCCGTCTGGCGCGCCAt > 1:2104267/1‑69 (MQ=255)
ttATCCGCGTTCATCTGCCGCCGCTGCGCGAACGTCGGGAAGATATTCCCCGTCTGGCGCGCCAttttt < 1:416901/69‑1 (MQ=255)
ttATCCGCGTTCATCTGCCGCCGCTGCGCGAACGTCGGGAAGATATTCCCCGTCTGGCGCGCCAttttt < 1:1172298/69‑1 (MQ=255)
|
CACCAGAATCTCGAACAGCGAGTGCAGGAAGGTAAGTTCCGTGAGGATCTGTTCCACCGCCTGAACGTTATCCGCGTTCATCTGCCGCCGCTGCGCGAACGTCGGGAAGATATTCCCCGTCTGGCGCGCCATTTTT > minE/2344175‑2344310
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
