Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,355,004 |
A→G |
32.8% |
N64N (AAT→AAC) |
dsbA ← |
periplasmic protein disulfide isomerase I |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,355,004 | 0 | A | G | 32.8%
| 104.7
/ 71.9
| 132 | N64N (AAT→AAC) | dsbA | periplasmic protein disulfide isomerase I |
| Reads supporting (aligned to +/- strand): ref base A (35/53); new base G (7/36); total (42/90) |
| Fisher's exact test for biased strand distribution p-value = 9.06e-03 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
Only 100 of 132 total aligned reads displayed.
CACCACCCATGAAGTTGACGTGGTATTTAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAGAACTTCTTCAAACTGATAGCAGTGCGGGCAGAAGAAAGAGAAA > minE/2354940‑2355064
|
caccacCCATGAAGTTGACGTGGTATTTAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACAtt > 1:1880703/1‑67 (MQ=255)
caccacCCATGAAGTTGACGTGGTATTTAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACAtt > 1:2892560/1‑67 (MQ=255)
caccacCCATGAAGTTGACGTGGTATTTAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACAtt > 1:1897659/1‑67 (MQ=255)
caccacCCATGAAGTTGACGTGGTATTTAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACAtt > 1:1913320/1‑67 (MQ=255)
caccacCCATGAAGTTGACGTGGTATTTAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACAtt > 1:2494500/1‑67 (MQ=255)
caccacCCATGAAGTTGACGTGGTATTTAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACAtt > 1:864150/1‑67 (MQ=255)
caccacCCATGAAGTTGACGTGGTATTTAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACAtt > 1:943559/1‑67 (MQ=255)
caccacCCATGAAGTTGACGTGGTATTTAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTAACAtt > 1:2471693/1‑67 (MQ=255)
gTTGACGTTGTATTTAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAg < 1:128768/69‑1 (MQ=255)
gTTGACGTGGTATTTAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACGTTATCAGAAATATGCa > 1:78522/1‑68 (MQ=255)
gTTGACGTGGTATTTAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACGTTATCAGAAATATGCAg > 1:2552337/1‑69 (MQ=255)
gTTGACGTGGTATTTAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACGTTATCAGAAATATGCAg > 1:3338475/1‑69 (MQ=255)
gTTGACGTGGTATTTAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACGTTATCAGAAATATGCAg > 1:233387/1‑69 (MQ=255)
gTTGACGTGGTATTTAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAg < 1:1460274/69‑1 (MQ=255)
gTTGACGTGGTATTTAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAg < 1:1649440/69‑1 (MQ=255)
gTTGACGTGGTATTTAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAg < 1:3391385/69‑1 (MQ=255)
gTTGACGTGGTATTTAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAg < 1:1839665/69‑1 (MQ=255)
gTTGACGTGGTATTTAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAg < 1:1842479/69‑1 (MQ=255)
gTTGACGTGGTATTTAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAg < 1:1589400/69‑1 (MQ=255)
gTTGACGTGGTATTTAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAg < 1:3333868/69‑1 (MQ=255)
gTTGACGTGGTATTTAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAg < 1:2397002/69‑1 (MQ=255)
gTTGACGTGGTATTTAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAg < 1:3301894/69‑1 (MQ=255)
gTTGACGTGGTATTTAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAg < 1:1019712/69‑1 (MQ=255)
gTTGACGTGGTATTTAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAg < 1:3059994/69‑1 (MQ=255)
gTATTTAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACTTTATCAGAAATATGCAGAACttcttc < 1:1654369/69‑1 (MQ=255)
gTATTTAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACGTTATCAGAAATATGCAGAACttcttc < 1:2186863/69‑1 (MQ=255)
gTATTTAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACGTTATCAGAAATATGCAGAACttcttc < 1:1008339/69‑1 (MQ=255)
gTATTTAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACGTTATCAGAAATATGCAGAACttcttc < 1:558745/69‑1 (MQ=255)
gTATTTAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACGTTATCAGAAATATGCAGAACttcttc < 1:2818165/69‑1 (MQ=255)
gTATTTAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACGTTATCAGAAATATGCAGAACttcttc < 1:2839914/69‑1 (MQ=255)
gTATTTAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACGTTATCAGAAATATGCAGAACttcttc < 1:501433/69‑1 (MQ=255)
gTATTTAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACGTTATCAGAAATATGCAGAACttcttc < 1:911244/69‑1 (MQ=255)
gTATTTAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACGTTATCAGAAATATGCAGAACttcttc < 1:1485681/69‑1 (MQ=255)
gTATTTAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACGTTATCAGAAATATGCAGAACttcttc < 1:1187207/69‑1 (MQ=255)
gTATTTAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACGTTATCAGAAATATGCAGAACttcttc < 1:408384/69‑1 (MQ=255)
tATTTAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACGTTATCAGAAATATGCAGAACttcttc < 1:3023795/68‑1 (MQ=255)
aTTTAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACGTTATCAGAAATATGCAGAACttcttc < 1:3211633/67‑1 (MQ=255)
aTTTAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAGAACTTCTTCa < 1:1842470/68‑1 (MQ=255)
aTTTAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAGAACTTCTTCa < 1:312978/68‑1 (MQ=255)
aTTTAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAGAACTTCTTCa < 1:630554/68‑1 (MQ=255)
aTTTAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAGAACTTCTTCa < 1:1963623/68‑1 (MQ=255)
aTTTAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAGAACTTCTTCa < 1:1550829/68‑1 (MQ=255)
aTTTAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAGAACTTCTTCa < 1:1324469/68‑1 (MQ=255)
tAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAGAACTTCTTCa > 1:368026/1‑65 (MQ=255)
tAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAGAACTTCTTCAAACt < 1:2907717/69‑1 (MQ=255)
tAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAGAACTTCTTCAAACt > 1:1059162/1‑69 (MQ=255)
tAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAGAACTTCTTCAAACt < 1:1377342/69‑1 (MQ=255)
tAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAGAACTTCTTCAAACt < 1:3166756/69‑1 (MQ=255)
tAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAGAACTTCTTCAAACt > 1:2534015/1‑69 (MQ=255)
tAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAGAACTTCTTCAAACt < 1:3386006/69‑1 (MQ=255)
tAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAGAACTTCTTCAAACt < 1:193459/69‑1 (MQ=255)
tAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAGAACTTCTTCAAACt > 1:2030234/1‑69 (MQ=255)
aGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAGAACTTCTTCAAACt < 1:340220/68‑1 (MQ=255)
aGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAGAACTTCTTCAAACt < 1:1496972/68‑1 (MQ=255)
aTCTTCACGCCTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAGAACTTCTTCAAACTGATAg < 1:537202/69‑1 (MQ=255)
aTCTTCACGCCTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAGAACTTCTTCAAACTGATAg < 1:1626957/69‑1 (MQ=255)
aTCTTCACGCCTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAGAACTTCTTCAAACTGATAg < 1:519911/69‑1 (MQ=255)
aTCTTCACGCCTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAGAACTTCTTCAAACTGATAg < 1:1447454/69‑1 (MQ=255)
aTCTTCACGCCTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAGAACTTCTTCAAACTGATAg < 1:2287927/69‑1 (MQ=255)
aTCTTCACGCCTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAGAACTTCTTCAAACTGATAg < 1:3254870/69‑1 (MQ=255)
aTCTTCACGCCTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAGAACTTCTTCAAACTGATAg < 1:273701/69‑1 (MQ=255)
aTCTTCACGCCTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAGAACTTCTTCAAACTGATAg < 1:3387410/69‑1 (MQ=255)
cTTCACGCCTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAGAACTTCTTCAAACTGATAGc < 1:2169569/68‑1 (MQ=255)
cTTCACGCCTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAGAACTTCTTCAAACTGATAGc < 1:2395682/68‑1 (MQ=255)
cTTCACGCCTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAGAACTTCTTCAAACTGATAGc < 1:1334913/68‑1 (MQ=255)
cTTCACGCCTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAGAACTTCTTCAAACTGATAGc < 1:2545771/68‑1 (MQ=255)
ttCACGCCTTCCGGCAGTTTTTTCTTCACATTATGAGAAATATGCAGAACTTCTTCAAACTGATAGCAg > 1:219449/1‑69 (MQ=255)
ttCACGCCTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAGAACTTCTTCa > 1:488971/1‑57 (MQ=255)
ttCACGCCTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAGAACTTCTTCa > 1:499455/1‑57 (MQ=255)
ttCACGCCTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAGAACTTCTTCa > 1:2894024/1‑57 (MQ=255)
ttCACGCCTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAGAACTTCTTCa > 1:2482710/1‑57 (MQ=255)
ttCACGCCTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAGAACTTCTTCa > 1:694012/1‑57 (MQ=255)
ttCACGCCTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAGAACTTCTTCAAACTGATAGCAg > 1:2212786/1‑69 (MQ=255)
ttCACGCCTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAGAACTTCTTCAAACTGATAGCAg > 1:3356334/1‑69 (MQ=255)
ttCACGCCTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAGAACTTCTTCAAACTGATAGCAg > 1:139052/1‑69 (MQ=255)
ttCACGCCTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAGAACTTCTTCAAACTGATAGCAg > 1:45033/1‑69 (MQ=255)
ttCACGCCTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAGAACTTCTTCAAACTGATAGCAg > 1:943684/1‑69 (MQ=255)
cTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAGAACTTCTTGAAACTGATAGCAGTGCg > 1:686654/1‑66 (MQ=255)
cTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAGAACTTCTTCAAACTGATAGCAGTGCg > 1:2756128/1‑66 (MQ=255)
cGGCAGTTTTTTCTTCACGTTATCAGAAATATGCAGAACttcttc < 1:1230644/45‑1 (MQ=255)
ttttCTTCACGTTATCAGAAATATGCAGAACTTCTTCAAACTGATAGc < 1:657001/48‑1 (MQ=255)
ttttCTTCACGTTATCAGAAATATGCAGAACTTCTTCAAACTGATAGc < 1:785771/48‑1 (MQ=255)
ttttCTTCACGTTATCAGAAATATGCAGAACTTCTTCAAACTGATAGc < 1:910729/48‑1 (MQ=255)
ttttCTTCACGTTATCAGAAATATGCAGAACTTCTTCAAACTGATAGc < 1:953957/48‑1 (MQ=255)
ttttCTTCACGTTATCAGAAATATGCAGAACTTCTTCAAACTGATAGc < 1:2945520/48‑1 (MQ=255)
ttttCTTCACGTTATCAGAAATATGCAGAACTTCTTCAAACTGATAGc < 1:2869843/48‑1 (MQ=255)
ttttCTTCACGTTATCAGAAATATGCAGAACTTCTTCAAACTGATAGc < 1:2768363/48‑1 (MQ=255)
ttttCTTCACGTTATCAGAAATATGCAGAACTTCTTCAAACTGATAGc < 1:2600096/48‑1 (MQ=255)
ttttCTTCACGTTATCAGAAATATGCAGAACTTCTTCAAACTGATAGc < 1:1957222/48‑1 (MQ=255)
ttttCTTCACGTTATCAGAAATATGCAGAACTTCTTCAAACTGATAGc < 1:1624850/48‑1 (MQ=255)
ttttCTTCACGTTATCAGAAATATGCAGAACTTCTTCAAACTGATAGc < 1:1430858/48‑1 (MQ=255)
ttttCTTCACGTTATCAGAAATATGCAGAACTTCTTCAAACTGATAGc < 1:101273/48‑1 (MQ=255)
tttCTTCACGTTATCAGAAATATGCAGAACTTCTTCAAACTGATAGc < 1:1403051/47‑1 (MQ=255)
tcttcACATTATCAGAAATATGCAGAACTTCTTCAAACTGATAGCAGTGCGGGCAGAAGAAAGAGaaa < 1:2951521/68‑1 (MQ=255)
tcttcACATTATCAGAAATATGCAGAACTTCTTCAAACTGATAGCAGTGCGGGCAGAAGAAAGAGaaa < 1:1890630/68‑1 (MQ=255)
tcttcACATTATCAGAAATATGCAGAACTTCTTCAAACTGATAGCAGTGCGGGCAGAAGAAAGAGaaa < 1:1863574/68‑1 (MQ=255)
tcttcACATTATCAGAAATATGCAGAACTTCTTCAAACTGATAGCAGTGCGGGCAGAAGAAAGAGaaa < 1:1859678/68‑1 (MQ=255)
tcttcACATTATCAGAAATATGCAGAACTTCTTCAAACTGATAGCAGTGCGGGCAGAAGAAAGAGaaa < 1:1762941/68‑1 (MQ=255)
tcttcACATTATCAGAAATATGCAGAACTTCTTCAAACTGATAGCAGTGCGGGCAGAAGAAAGAGaaa < 1:1759056/68‑1 (MQ=255)
tcttcACATTATCAGAAATATGCAGAACTTCTTCAAACTGATAGCAGTGCGGGCAGAAGAAAGAGaaa < 1:1154492/68‑1 (MQ=255)
|
CACCACCCATGAAGTTGACGTGGTATTTAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAGAACTTCTTCAAACTGATAGCAGTGCGGGCAGAAGAAAGAGAAA > minE/2354940‑2355064
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
