Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,416,428 |
A→G |
40.5% |
V433V (GTT→GTC) |
yifK ← |
predicted transporter |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,416,428 | 0 | A | G | 40.5%
| 43.2
/ 76.7
| 106 | V433V (GTT→GTC) | yifK | predicted transporter |
| Reads supporting (aligned to +/- strand): ref base A (29/34); new base G (18/25); total (47/59) |
| Fisher's exact test for biased strand distribution p-value = 6.95e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.12e-01 |
Only 100 of 107 total aligned reads displayed.
GCGATTAAGGCCAAAAACTTTATAAATCGCCGTCACCGCCAGCATAAAGATGATGCCAACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCAAAACGCAAATCAGGAATGCC > minE/2416371‑2416494
|
gCGATTAAGGCCAAAAACTTTATAAATCGCCGTCACCGCCAGCATAAAGATGATGCcaacaa > 1:2737078/1‑62 (MQ=255)
gCGATTAAGGCCAAAAACTTTATAAATCGCCGTCACCGCCAGCATAAAGATGATGCCAACAAACAGCg > 1:3173859/1‑68 (MQ=255)
gCGATTAAGGCCAAAAACTTTATAAATCGCCGTCACCGCCAGCATAAAGATGATGCCAACAAACAGCGa > 1:708907/1‑69 (MQ=255)
gCGATTAAGGCCAAAAACTTTATAAATCGCCGTCACCGCCAGCATAAAGATGATGCCAACAAACAGCGa > 1:2996895/1‑69 (MQ=255)
gCGATTAAGGCCAAAAACTTTATAAATCGCCGTCACCGCCAGCATAAAGATGATGCCAACAAACAGCGa > 1:1067114/1‑69 (MQ=255)
gCGATTAAGGCCAAAAACTTTATAAATCGCCGTCACCGCCAGCATAAAGATGATGCCAACAAACAGCGa > 1:2302054/1‑69 (MQ=255)
gCGATTAAGGCCAAAAACTTTATAAATCGCCGTCACCGCCAGCATAAAGATGATGCCAACAAACAGCGa > 1:3317196/1‑69 (MQ=255)
gCCAAAAACTTTATAAATCGCCGTCACCGCCAGCATAAAGATGATGCCAACAAACAGCGACATACGCGt < 1:1108180/69‑1 (MQ=255)
gCCAAAAACTTTATAAATCGCCGTCACCGCCAGCATAAAGATGATGCCAACAAACAGCGACATACGCGt < 1:3230078/69‑1 (MQ=255)
gCCAAAAACTTTATAAATCGCCGTCACCGCCAGCATAAAGATGATGCCAACAAACAGCGACATACGCGt < 1:3207909/69‑1 (MQ=255)
gCCAAAAACTTTATAAATCGCCGTCACCGCCAGCATAAAGATGATGCCAACAAACAGCGACATACGCGt < 1:2293208/69‑1 (MQ=255)
aaTCGCCGTCACCGCCAGCATAAAGATGATGCCAACAAACAGCGACATACGCGTATCTTCATTAAAGt < 1:2174302/68‑1 (MQ=255)
aaTCGCCGTCACCGCCAGCATAAAGATGATGCCAACAAACAGCGACATACGCGTATCTTCATTAAAGt < 1:3161529/68‑1 (MQ=255)
aaTCGCCGTCACCGCCAGCATAAAGATGATGCCAACAAACAGCGACATACGCGTATCTTCATTAAAGt < 1:504283/68‑1 (MQ=255)
aaTCGCCGTCACCGCCAGCATAAAGATGATGCCAACAAACAGCGACATACGCGTATCTTCATTAAAGTa < 1:1142914/69‑1 (MQ=255)
aaTCGCCGTCACCGCCAGCATAAAGATGATGCCAACAAACAGCGACATACGCGTATCTTCATTAAAGTa < 1:588787/69‑1 (MQ=255)
aaTCGCCGTCACCGCCAGCATAAAGATGATGCCAACAAACAGCGACATACGCGTATCTTCATTAAAGTa < 1:2038280/69‑1 (MQ=255)
aaTCGCCGTCACCGCCAGCATAAAGATGATGCCAACAAACAGCGACATACGCGTATCTTCATTAAAGTa < 1:3039090/69‑1 (MQ=255)
aaTCGCCGTCACCGCCAGCATAAAGATGATGCCAACAAACAGCGACATACGCGTATCTTCATTAAAGTa < 1:2175219/69‑1 (MQ=255)
aaTCGCCGTCACCGCCAGCATAAAGATGATGCCAACAAACAGCGACATACGCGTATCTTCATTAAAGTa < 1:2430118/69‑1 (MQ=255)
gTCACCACCAGCATGAAGATGATGCCGACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCg > 1:3199654/1‑69 (MQ=38)
gTCACCACCAGCATAAAGATGATGCCGACAAACAGCGACATGCGCGTATCTTCATTAAAGTACATGCCg > 1:1231890/1‑69 (MQ=255)
gTCACCACCAGCATAAAGATGATGCCGACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCg > 1:2011772/1‑69 (MQ=255)
gTCACCACCAGCATAAAGATGATGCCGACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCg > 1:1803714/1‑69 (MQ=255)
gTCACCACCAGCATAAAGATGATGCCGACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCg > 1:1998110/1‑69 (MQ=255)
gTCACCACCAGCATAAAGATGATGCCGACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCg > 1:1853960/1‑69 (MQ=255)
gTCACCACCAGCATAAAGATGATGCCGACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCg > 1:1041032/1‑69 (MQ=255)
gTCACCACCAGCATAAAGATGATGCCGACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCg > 1:227917/1‑69 (MQ=255)
gTCACCACCAGCATAAAGATGATGCCGACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCg > 1:2413419/1‑69 (MQ=255)
gTCACCACCAGCATAAAGATGATGCCGACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCg > 1:685925/1‑69 (MQ=255)
gTCACCACCAGCATAAAGATGATGCCGACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCg > 1:700242/1‑69 (MQ=255)
gTCACCACCAGCATAAAGATGATGCCGACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCg > 1:744235/1‑69 (MQ=255)
gTCACCACCAGCATAAAGATGATGCCGACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCg > 1:1100072/1‑69 (MQ=255)
gTCACCACCAGCATAAAGATGATGCCGACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCg > 1:1393483/1‑69 (MQ=255)
gTCACCACCAGCATAAAGATGATGCCGACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCg > 1:1246316/1‑69 (MQ=255)
gTCACCACCAGCATAAAGATGATGCCGACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCg > 1:1122509/1‑69 (MQ=255)
gTCACCACCAGCATAAAGATGATGCCGACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCg > 1:1080373/1‑69 (MQ=255)
aCCGCCAGCATAAAGATGATGCCAACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGa > 1:326739/1‑67 (MQ=255)
aCCGCCAGCATAAAGATGATGCCAACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGAt > 1:3011583/1‑68 (MQ=255)
aCCGCCAGCATAAAGATGATGCCAACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGAt > 1:1119876/1‑68 (MQ=255)
aCCGCCAGCATAAAGATGATGCCAACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGAt > 1:2993186/1‑68 (MQ=255)
aCCGCCAGCATAAAGATGATGCCAACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGAt > 1:1522842/1‑68 (MQ=255)
aCCGCCAGCATAAAGATGATGCCAACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGAt > 1:3204486/1‑68 (MQ=255)
aCCGCCAGCATAAAGATGATGCCAACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGAt > 1:100299/1‑68 (MQ=255)
aCCGCCAGCATAAAGATGATGCCAACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGAt > 1:1491723/1‑68 (MQ=255)
aaGATTATGCCAACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCAAAACGCAAATc > 1:1390209/1‑69 (MQ=255)
aaGATGATGCCGACAAACAGCGACATACGCGTATCTTCTTTAAAGTACATGCCGATCAAAACg < 1:450425/63‑1 (MQ=39)
aaGATGATGCCGACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCTAAACg < 1:1830597/63‑1 (MQ=255)
aaGATGATGCCGACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCAAAACg < 1:1237926/63‑1 (MQ=255)
aaGATGATGCCGACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCAAAACg < 1:2868114/63‑1 (MQ=255)
aaGATGATGCCGACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCAAAACg < 1:3036308/63‑1 (MQ=255)
aaGATGATGCCGACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCAAAACg < 1:1789447/63‑1 (MQ=255)
aaGATGATGCCGACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCAAAACg < 1:3197595/63‑1 (MQ=255)
aaGATGATGCCGACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCAAAACg < 1:1182170/63‑1 (MQ=255)
aaGATGATGCCGACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCAAAACg < 1:566603/63‑1 (MQ=255)
aaGATGATGCCGACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCAAAACg < 1:1079254/63‑1 (MQ=255)
aaGATGATGCCGACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCAAAACg < 1:1045552/63‑1 (MQ=255)
aaGATGATGCCGACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCAAAACg < 1:766707/63‑1 (MQ=255)
aaGATGATGCCGACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCAAAACg < 1:1785537/63‑1 (MQ=255)
aaGATGATGCCGACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCAAAACg < 1:2643713/63‑1 (MQ=255)
aaGATGATGCCGACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCAAAACg < 1:180851/63‑1 (MQ=255)
aaGATGATGCCGACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCAAAACg < 1:2605561/63‑1 (MQ=255)
aaGATGATGCCGACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCAAAACg < 1:2590122/63‑1 (MQ=255)
aaGATGATGCCGACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCAAAACg < 1:2453466/63‑1 (MQ=255)
aaGATGATGCCGACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCAAAACg < 1:1973274/63‑1 (MQ=255)
aaGATGATGCCGACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCAAAACg < 1:1291306/63‑1 (MQ=255)
aaGATGATGCCAACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCAAAACGCAAATc > 1:2805997/1‑69 (MQ=255)
aaGATGATGCCAACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCAAAACGCAAATc > 1:1050796/1‑69 (MQ=255)
aaGATGATGCCAACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCAAAACGCAAATc > 1:489685/1‑69 (MQ=255)
aaGATGATGCCAACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCAAAACGCAAATc > 1:447042/1‑69 (MQ=255)
aaGATGATGCCAACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCAAAACGCAAATc > 1:424220/1‑69 (MQ=255)
aaGATGATGCCAACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCAAAACGCAAATc > 1:3430242/1‑69 (MQ=255)
aaGATGATGCCAACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCAAAACGCAAATc > 1:3235285/1‑69 (MQ=255)
aaGATGATGCCAACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCAAAACGCAAATc > 1:136145/1‑69 (MQ=255)
aaGATGATGCCAACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCAAAACGCAAATc > 1:1266432/1‑69 (MQ=255)
aaGATGATGCCAACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCAAAACGCAAATc > 1:3081684/1‑69 (MQ=255)
aaGATGATGCCAACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCAAAACGCAAATc > 1:3080231/1‑69 (MQ=255)
aaGATGATGCCAACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCAAAACGCAAATc > 1:1259949/1‑69 (MQ=255)
aaGATGATGCCAACAAACAGCGACATACGCGAATCTTCATTAAAGTACATGCCGATCa > 1:2913244/1‑58 (MQ=255)
atgatgCCAACAAACAGCGACATACGCTTATCTTCATTAAAGTACATGCCGATCAAAACGCAAATCAgg < 1:1587065/69‑1 (MQ=255)
atgatgCCAACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCAAAACGCAAATCAgg < 1:2266265/69‑1 (MQ=255)
atgatgCCAACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCAAAACGCAAATCAgg < 1:850154/69‑1 (MQ=255)
atgatgCCAACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCAAAACGCAAATCAgg < 1:1739540/69‑1 (MQ=255)
atgatgCCAACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCAAAACGCAAATCAgg < 1:1074572/69‑1 (MQ=255)
atgatgCCAACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCAAAACGCAAATCAgg < 1:647289/69‑1 (MQ=255)
atgatgCCAACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCAAAACGCAAATCAgg < 1:61070/69‑1 (MQ=255)
atgatgCCAACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCAAAACGCAAATCAgg < 1:1665772/69‑1 (MQ=255)
atgatgCCAACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCAAAACGCAAATCAgg < 1:2986575/69‑1 (MQ=255)
atgatgCCAACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCAAAACGCAAATCAgg < 1:3432373/69‑1 (MQ=255)
atgatgCCAACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCAAAACGCAAATCAgg < 1:3302414/69‑1 (MQ=255)
atgatgCCAACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCAAAACGCAAATCAgg < 1:1654498/69‑1 (MQ=255)
atgatgCCAACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCAAAACGCAAATCAgg < 1:3114590/69‑1 (MQ=255)
atgatgCCAACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCAAAACGCAAATCAgg < 1:1527794/69‑1 (MQ=255)
atgatgCCAACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCAAAACGCAAATCAgg < 1:1496909/69‑1 (MQ=255)
atgatgCCAACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCAAAACGCAAATCAgg < 1:1216765/69‑1 (MQ=255)
tgatgCCAACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCAAAACGCAAATCAgg < 1:2929216/68‑1 (MQ=255)
gatgCCGACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCAAAACg < 1:194919/58‑1 (MQ=255)
gCCAACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCAAAACGCAAATCAGGAATGc < 1:1879162/69‑1 (MQ=255)
gCCAACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCAAAACGCAAATCAGGAATGc < 1:1837761/69‑1 (MQ=255)
ccAACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCAAAACGCAAATCAGGAATGcc > 1:2558072/1‑69 (MQ=255)
|
GCGATTAAGGCCAAAAACTTTATAAATCGCCGTCACCGCCAGCATAAAGATGATGCCAACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCAAAACGCAAATCAGGAATGCC > minE/2416371‑2416494
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
