Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,431,447 |
G→C |
49.3% |
A250A (GCC→GCG) |
rho ← |
transcription termination factor |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,431,447 | 0 | G | C | 49.3%
| ‑1.5
/ 88.5
| 71 | A250A (GCC→GCG) | rho | transcription termination factor |
| Reads supporting (aligned to +/- strand): ref base G (26/10); new base C (13/22); total (39/32) |
| Fisher's exact test for biased strand distribution p-value = 4.20e-03 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-04 |
CGCGCGCCAGACGAGTGATGGAGTCGAGCAGAATGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTGGCCTTCTCGATCACCATTTCCGCAACCTGAACGTGGCGAGATGCGGGTTCGTCAAAGGTAGAAGCA > minE/2431380‑2431513
|
cgcgcgcCAGACGAGTGATGGAGTCGAGCAGAATGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTg < 1:2285803/68‑1 (MQ=255)
cgcgcgcCAGACGAGTGATGGAGTCGAGCAGAATGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTg < 1:819680/68‑1 (MQ=255)
cgcgcgcCAGACGAGTGATGGAGTCGAGCAGAATGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTg < 1:111217/68‑1 (MQ=255)
cgcgcgcCAGACGAGTGATGGAGTCGAGCAGAATGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTg < 1:2764129/68‑1 (MQ=255)
cgcgcgcCAGACGAGTGATGGAGTCGAGCAGAATGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTg < 1:1946368/68‑1 (MQ=255)
cgcgcgcCAGACGAGTGATGGAGTCGAGCAGAATGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTg < 1:3281599/68‑1 (MQ=255)
cgcgcgcCAGACGAGTGATGGAGTCGAGCAGAATGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTg < 1:161956/68‑1 (MQ=255)
cgcgcgcCAGACGAGTGATGGAGTCGAGCAGAATGAAAACGTCTTTCTTGTGCTCAACCAGGCGTTTg < 1:898733/68‑1 (MQ=255)
gcgcgcCAGACGAGTGATGGAGTCGAGCAGAATGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTGGc < 1:403137/69‑1 (MQ=255)
cgcgcCAGACGAGTGATGGAGTCGAGCAGAATGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTCGcc < 1:983704/69‑1 (MQ=255)
cgcgcCAGACGAGTGATGGAGTCGAGCAGAATGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTCGcc < 1:2468170/69‑1 (MQ=255)
cgcgcCAGACGAGTGATGGAGTCGAGCAGAATGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTCGcc < 1:2350951/69‑1 (MQ=255)
cgcgcCAGACGAGTGATGGAGTCGAGCAGAATGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTCGcc < 1:2702986/69‑1 (MQ=255)
cgcgcCAGACGAGTGATGGAGTCGAGCAGAATGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTCGcc < 1:2734469/69‑1 (MQ=255)
cgcgcCAGACGAGTGATGGAGTCGAGCAGAATGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTCGcc < 1:3012656/69‑1 (MQ=255)
cgcgcCAGACGAGTGATGGAGTCGAGCAGAATGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTCGcc < 1:84072/69‑1 (MQ=255)
cgcgcCAGACGAGTGATGGAGTCGAGCAGAATGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTCGcc < 1:560695/69‑1 (MQ=255)
gcgcCAGACGAGTGATGGAGTCGAGCAGAATGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTCGcc < 1:53454/68‑1 (MQ=255)
gcgcCAGACGAGTGATGGAGTCGAGCAGAATGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTCGcc < 1:640872/68‑1 (MQ=255)
gcgcCAGACGAGTGATGGAGTCGAGCAGAATGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTCGcc < 1:2119389/68‑1 (MQ=255)
gcgcCAGACGAGTGATGGAGTCGAGCAGAATGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTCGcc < 1:3418831/68‑1 (MQ=255)
gcgcCAGACGAGTGATGGAGTCGAGCAGAATGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTCGcc < 1:1768078/68‑1 (MQ=255)
gcgcCAGACGAGTGATGGAGTCGAGCAGAATGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTCGcc < 1:1634499/68‑1 (MQ=255)
cgcCAGACGAGTGATGGAGTCGAGCAGAATGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTCGcc < 1:1281360/67‑1 (MQ=255)
aGACGAGTGATGGAGTCGAGCAGAATGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTGGCCTTCTCg < 1:354956/69‑1 (MQ=255)
tGATGGAGTCGAGCAGAATGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTg < 1:1972342/53‑1 (MQ=255)
ggAGTCGAGCAGAATGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTCGCCTTCTCGATCACCAttt < 1:1501961/68‑1 (MQ=255)
ggAGTCGAGCAGAATGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTCGCCTTCTCGATCACCAttt < 1:510919/68‑1 (MQ=255)
ggAGTCGAGCAGAATGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTCGCCTTCTCGATCACCAttt < 1:1916951/68‑1 (MQ=255)
ggAGTCGAGCAGAATGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTCGCCTTCTCGATCACCAttt < 1:1940405/68‑1 (MQ=255)
ggAGTCGAGCAGAATGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTCGCCTTCTCGATCACCAttt < 1:841179/68‑1 (MQ=255)
ggAGTCGAGCAGAATGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTCGCCTTCTCGATCACCAttt < 1:2043450/68‑1 (MQ=255)
gAGTCGAGCAGAATGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTCGCCTTCTCGATCACCAttt < 1:505362/67‑1 (MQ=255)
gCAGAATGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTGGCCTTCTCGATCACCATTTCCGCa < 1:98820/65‑1 (MQ=255)
gCAGAATGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTGGCCTTCTCGATCACCATTTCCGCa < 1:2661326/65‑1 (MQ=255)
gCAGAATGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTGGCCTTCTCGATCACCATTTCCGCa < 1:2799510/65‑1 (MQ=255)
gCAGAATGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTGGCCTTCTCGATCACCATTTCCGCa < 1:2825073/65‑1 (MQ=255)
gCAGAATGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTGGCCTTCTCGATCACCATTTCCGCa < 1:3293255/65‑1 (MQ=255)
gCAGAATGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTGGCCTTCTCGATCACCATTTCCGCa < 1:2151712/65‑1 (MQ=255)
tGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTGGCCt > 1:212695/1‑39 (MQ=255)
tGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTGGCCTtctc > 1:2951337/1‑43 (MQ=255)
tGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTGGCCTTCTCg > 1:1991852/1‑44 (MQ=255)
tGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTGGCCTTCTCg > 1:1825315/1‑44 (MQ=255)
tGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTGGCCTTCTCg > 1:461701/1‑44 (MQ=255)
tGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTGGCCTTCTCg > 1:1490704/1‑44 (MQ=255)
tGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTGGCCTTCTCg > 1:2736259/1‑44 (MQ=255)
tGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTGGCCTTCTCg > 1:2732191/1‑44 (MQ=255)
tGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTGGCCTTCTCg > 1:2188951/1‑44 (MQ=255)
tGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTGGCCTTCTCg > 1:1097089/1‑44 (MQ=255)
tGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTGGCCTTCTCGATCACCATTTCCGCa > 1:80142/1‑59 (MQ=255)
tGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTGGCCTTCTCGATCACCATTTCCGCa > 1:776902/1‑59 (MQ=255)
tGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTGGCCTTCTCGATCACCATTTCCGCa > 1:1224893/1‑59 (MQ=255)
tGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTGGCCTTCTCGATCACCATTTCCGCAACCTGAACgg < 1:1081593/69‑2 (MQ=255)
tGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTGGCCTTCTCGATCACCATTTCCGCAACCTGAACGt < 1:1502553/69‑1 (MQ=255)
tctttcttGTGCTCAACCAGGCGTTTGGCCTTCTCGATCACCATTTCCGCAACCTGAACGTGGCGAGAt > 1:1090296/1‑69 (MQ=255)
tttcttGTGCTCAACCAGGCGTTTGGCCTTCTCGATCACCATTTCCGCAACCTGAACGTGGCGAGATGc > 1:1827653/1‑69 (MQ=255)
tttcttGTGCTCAACCAGGCGTTTGGCCTTCTCGATCACCATTTCCGCAACCTGAACGTGGCGAGATGc > 1:1339923/1‑69 (MQ=255)
ttGTGCTCAACCAGGCGTTTCGCCTTCTCGATCACCATTTc > 1:1630143/1‑41 (MQ=255)
ttGTGCTCAACCAGGCGTTTCGCCTTCTCGATCACCATTTCCg > 1:2880897/1‑43 (MQ=255)
ttGTGCTCAACCAGGCGTTTCGCCTTCTCGATCACCATTTCCGCa > 1:3296389/1‑45 (MQ=255)
ttGTGCTCAACCAGGCGTTTCGCCTTCTCGATCACCATTTCCGCa > 1:1809698/1‑45 (MQ=255)
ttGTGCTCAACCAGGCGTTTCGCCTTCTCGATCACCATTTCCGCa > 1:2593440/1‑45 (MQ=255)
ttGTGCTCAACCAGGCGTTTCGCCTTCTCGATCACCATTTCCGCa > 1:393965/1‑45 (MQ=255)
ttGTGCTCAACCAGGCGTTTCGCCTTCTCGATCACCATTTCCGCa > 1:419428/1‑45 (MQ=255)
ttGTGCTCAACCAGGCGTTTCGCCTTCTCGATCACCATTTCCGCa > 1:1951261/1‑45 (MQ=255)
ttGTGCTCAACCAGGCGTTTCGCCTTCTCGATCACCATTTCCGCa > 1:1162314/1‑45 (MQ=255)
ttGTGCTCAACCAGGCGTTTCGCCTTCTCGATCACCATTTCCGCa > 1:664222/1‑45 (MQ=255)
ttGTGCTCAACCAGGCGTTTCGCCTTCTCGATCACCATTTCCGCa > 1:1036849/1‑45 (MQ=255)
ttGTGCTCAACCAGGCGTTTCGCCTTCTCGATCACCATTTCCGCa > 1:2770737/1‑45 (MQ=255)
ttGTGCTCAACCAGGCGTTTCGCCTTCTCGATCACCATTTCCGCa > 1:2309911/1‑45 (MQ=255)
tGGCCTTCTCGATCACCATTTCCGCAACCTGAACGTGGCGAGATGCGGGTTCGTCAAAGGTAGAAGca > 1:1753035/1‑68 (MQ=255)
tGGCCTTCTCGATCACCATTTCCGCAACCTGAACGTGGCGAGATGCGGGTTCGTCAAAGGTAGAAGca > 1:896062/1‑68 (MQ=255)
tGGCCTTCTCGATCACCATTTCCGCAACCTGAACGTGGCGAGATGCGGGTTCGTCAAAGGTAGAAGca > 1:919439/1‑68 (MQ=255)
tGGCCTTCTCGATCACCATTTCCGCAACCTGAACGTGGCGAGATGCGGGTTCGTCAAAGGTAGAAGca > 1:3196072/1‑68 (MQ=255)
tGGCCTTCTCGATCACCATTTCCGCAACCTGAACGTGGCGAGATGCGGGTTCGTCAAAGGTAGAAGca > 1:3105279/1‑68 (MQ=255)
tGGCCTTCTCGATCACCATTTCCGCAACCTGAACGTGGCGAGATGCGGGTTCGTCAAAGGTAGAAGca > 1:3014489/1‑68 (MQ=255)
tGGCCTTCTCGATCACCATTTCCGCAACCTGAACGTGGCGAGATGCGGGTTCGTCAAAGGTAGAAGca > 1:1910222/1‑68 (MQ=255)
tGGCCTTCTCGATCACCATTTCCGCAACCTGAACGTGGCGAGATGCGGGTTCGTCAAAGGTAGAAGca > 1:2781633/1‑68 (MQ=255)
tGGCCTTCTCGATAACCATTTCCGCAACCTGAACGTGGCGAGATGCGGGTTCGTCAAAGGTAGAAGca > 1:1092729/1‑68 (MQ=255)
tGGACTTCTCGATCACCATTTCCGCAACCTGAACGTGGCGAGATGCGGGTTCGTCAAAGGTAGAAGca > 1:1028204/1‑68 (MQ=255)
|
CGCGCGCCAGACGAGTGATGGAGTCGAGCAGAATGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTGGCCTTCTCGATCACCATTTCCGCAACCTGAACGTGGCGAGATGCGGGTTCGTCAAAGGTAGAAGCA > minE/2431380‑2431513
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
