Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F1 I0 R1 4577 57.4 3436498 77.7% 2670158 63.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,459,805 T→C 45.0% L139L (TTG→CTG)  asnC → DNA‑binding transcriptional dual regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,459,8050TC45.0% 18.1 / 91.1 110L139L (TTG→CTG) asnCDNA‑binding transcriptional dual regulator
Reads supporting (aligned to +/- strand):  ref base T (21/39);  new base C (35/14);  total (57/53)
Fisher's exact test for biased strand distribution p-value = 2.25e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 110 total aligned reads displayed.

ACGCTCTCCAGCATGTACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACATTGATCGTCCTGCAAAACCCGATCATGCGTACCATCAAGCCCTGATCGGCTTTTTTAATCCCA  >  minE/2459740‑2459867
                                                                 |                                                              
atgCTCTCTAGCATGTACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACACTGa                                                             >  1:3139313/3‑69 (MQ=255)
atgCTCTCCAGCATGTACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACACTg                                                              >  1:3028106/3‑68 (MQ=255)
atgCTCTCCAGCATGTACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACACTGa                                                             >  1:1969348/3‑69 (MQ=255)
atgCTCTCCAGCATGTACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACACTGa                                                             >  1:2567415/3‑69 (MQ=255)
atgCTCTCCAGCATGTACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACACTGa                                                             >  1:2071754/3‑69 (MQ=255)
atgCTCTCCAGCATGTACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACACTGa                                                             >  1:213100/3‑69 (MQ=255)
atgCTCTCCAGCATGTACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACACTGa                                                             >  1:2150802/3‑69 (MQ=255)
atgCTCTCCAGCATGTACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACACTGa                                                             >  1:2521574/3‑69 (MQ=255)
atgCTCTCCAGCATGTACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACACTGa                                                             >  1:1008108/3‑69 (MQ=255)
atgCTCTCCAGCATGTACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACACTGa                                                             >  1:2568149/3‑69 (MQ=255)
atgCTCTCCAGCATGTACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACACTGa                                                             >  1:2605412/3‑69 (MQ=255)
atgCTCTCCAGCATGTACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACACTGa                                                             >  1:2641915/3‑69 (MQ=255)
atgCTCTCCAGCATGTACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACACTGa                                                             >  1:2721869/3‑69 (MQ=255)
atgCTCTCCAGCATGTACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACACTGa                                                             >  1:2885493/3‑69 (MQ=255)
atgCTCTCCAGCATGTACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACACTGa                                                             >  1:3409979/3‑69 (MQ=255)
atgCTCTCCAGCATGTACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACACTGa                                                             >  1:3424661/3‑69 (MQ=255)
atgCTCTCCAGCATGTACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACACTGa                                                             >  1:435239/3‑69 (MQ=255)
atgCTCTCCAGCATGTACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACACTGa                                                             >  1:491692/3‑69 (MQ=255)
atgCTCTCCAGCATGTACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACACTGa                                                             >  1:565616/3‑69 (MQ=255)
atgCTCTCCAGCATGTACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACACTGa                                                             >  1:63847/3‑69 (MQ=255)
atgCTCTCCAGCATGTACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACACTGa                                                             >  1:670277/3‑69 (MQ=255)
atgCTCTCCAGCATGTACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACACTGa                                                             >  1:698104/3‑69 (MQ=255)
atgCTCTCCAGCATGTACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACACTGa                                                             >  1:728779/3‑69 (MQ=255)
atgCTCTCCAGCATGTACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACACTGa                                                             >  1:947690/3‑69 (MQ=255)
atgCTCTCCAGCATGTACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACACTGa                                                             >  1:995422/3‑69 (MQ=255)
atgCTCTCCAGCATGTACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACACTGa                                                             >  1:1919268/3‑69 (MQ=255)
atgCTCTCCAGCATGTACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACACTGa                                                             >  1:1673212/3‑69 (MQ=255)
atgCTCTCCAGCATGTACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACACTGa                                                             >  1:125977/3‑69 (MQ=255)
atgCTCTCAAGCATGTACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACACTGa                                                             >  1:1304179/3‑69 (MQ=255)
          gCATGTACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACACTGATCGTCCTGc                                                    <  1:2131359/68‑1 (MQ=255)
          gCATGTACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACACTGATCGTCCTGc                                                    <  1:2661010/68‑1 (MQ=255)
          gCATGTACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACACTGATCGTCCTGc                                                    <  1:3408064/68‑1 (MQ=255)
          gCATGTACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACACTGATCGTCCTGc                                                    <  1:453090/68‑1 (MQ=255)
          gCATGTACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACACTGATCGTCCTGc                                                    <  1:1219061/68‑1 (MQ=255)
               tACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACATTGATCGTCCTGCaaa                                                 >  1:846236/1‑66 (MQ=255)
               tACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACATTGATCGTCCTGCAAAAc                                               >  1:541394/1‑68 (MQ=255)
               tACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACATTGATCGTCCTGCAAAAc                                               >  1:465916/1‑68 (MQ=255)
               tACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACATTGATCGTCCTGCAAAAc                                               >  1:1918691/1‑68 (MQ=255)
               tACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACATTGATCGTCCTGCAAAAc                                               >  1:1081577/1‑68 (MQ=255)
               tACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACATTGATCGTCCTGCAAAAc                                               >  1:105792/1‑68 (MQ=255)
               tACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACATTGATCGTCCTGCAAAAc                                               >  1:3201765/1‑68 (MQ=255)
               tACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACATTGATCGTCCTGCAAAAc                                               >  1:2780664/1‑68 (MQ=255)
               tACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACATTGATCGTCCTGCAAAAc                                               >  1:1049525/1‑68 (MQ=255)
               tACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACATTGATCGTCCTGCAAAAc                                               >  1:1466066/1‑68 (MQ=255)
               tACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACATTGATCGTCCTGCAAAAc                                               >  1:1026843/1‑68 (MQ=255)
               tACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACATTGATCGTCCTGCAAAAc                                               >  1:2488476/1‑68 (MQ=255)
               tACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACATTGATCGTCCTGCAAAAc                                               >  1:2318443/1‑68 (MQ=255)
               tACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACATTGATCGTCCTGCAAAAc                                               >  1:2201924/1‑68 (MQ=255)
               tACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACATTGATCGTCCTGCAAAAc                                               >  1:2034157/1‑68 (MQ=255)
               tACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACATTGATCGTCCTGCAAAAc                                               >  1:2115929/1‑68 (MQ=255)
               tACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACATTGATCGTCCTGCAAAAc                                               >  1:2289717/1‑68 (MQ=255)
               tACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTACACCGAGACATTGATCGTCCTGCAAAAc                                               >  1:944057/1‑68 (MQ=255)
               tACTTATCAACAAGATCCAAACAATTGATGAAAATCAGTCCACCGAGACATAGAt                                                            >  1:2585802/1‑55 (MQ=255)
               tACTAATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACAATGATCGTCCTGCaaaa                                                >  1:2407661/1‑67 (MQ=255)
                   tATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACATTGATCGTCCTGCAAAACCCGa                                           <  1:2289238/68‑1 (MQ=255)
                   tATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACATTGATCGTCCTGCAAAACCCGAt                                          <  1:203391/69‑1 (MQ=255)
                   tATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACATTGATCGTCCTGCAAAACCCGAt                                          <  1:1710701/69‑1 (MQ=255)
                   tATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACATTGATCGTCCTGCAAAACCCGAt                                          <  1:388166/69‑1 (MQ=255)
                   tATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACATTGATCGTCCTGCAAAACCCGAt                                          <  1:1075122/69‑1 (MQ=255)
                   tATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACATTGATAGTCCTGCAAAACCCGAt                                          <  1:3356268/69‑1 (MQ=255)
                       aacaaGATCCAAACAATTGATGAAATTCAGTCCACCGAGACATTGATCGTCCTGCAAAACCCGATCATg                                      <  1:2763545/69‑1 (MQ=255)
                       aacaaGATCCAAACAATTGATGAAATTCAGTCCACCGAGACATTGATCGTCCTGCAAAACCCGATCATg                                      <  1:3054075/69‑1 (MQ=255)
                       aacaaGATCCAAACAATTGATGAAATTCAGTCCACCGAGACATTGATCGTCCTGCAAAACCCGATCATg                                      <  1:1268238/69‑1 (MQ=255)
                         caaGATCCAAACAATTGATGAAATTCAGTCCACCGAGACATTGATCGTCCTGCAAAACCCGAt                                          >  1:2614924/1‑63 (MQ=255)
                             aTCCAAACGATTGATGAAATTCAGTCCACCGAGACATTGATCGTCCTGCAAAACCCGATCATGCGTAcc                                <  1:839994/69‑1 (MQ=255)
                             aTCCAAACAATTGATTAAATTCAGTCCACCGAGACATTGATCGTCCTGCAAAACCCGATCATGCGTAcc                                <  1:1244697/69‑1 (MQ=255)
                             aTCCAAACAATTGATGAAATTCAGTCCACCGATACATTGATCGTCCTGCAAAACCCGATCATGCGTAcc                                <  1:1548229/69‑1 (MQ=255)
                             aTCCAAACAATTGATGAAATTCAGTCCACCGAGACATTGATCGTCCTGCAAAACCCGATCATGCGTAcc                                <  1:1907802/69‑1 (MQ=255)
                             aTCCAAACAATTGATGAAATTCAGTCCACCGAGACATTGATCGTCCTGCAAAACCCGATCATGCGTAcc                                <  1:638577/69‑1 (MQ=255)
                             aTCCAAACAATTGATGAAATTCAGTCCACCGAGACATTGATCGTCCTGCAAAACCCGATCATGCGTAcc                                <  1:1219108/69‑1 (MQ=255)
                             aTCCAAACAATTGATGAAATTCAGTCCACCGAGACATTGATCGTCCTGCAAAACCCGATCATGCGTAcc                                <  1:935865/69‑1 (MQ=255)
                             aTCCAAACAATTGATGAAATTCAGTCCACCGAGACATTGATCGTCCTGCAAAACCCGATCATGCGTAcc                                <  1:238313/69‑1 (MQ=255)
                             aTCCAAACAATTGATGAAATTCAGTCCACCGAGACATTGATCGTCCTGCAAAACCCGATCATGCGTAcc                                <  1:2194119/69‑1 (MQ=255)
                             aTCCAAACAATTGATGAAATTCAGTCCACCGAGACATTGATCGTCCTGCAAAACCCGATCATGCGTAcc                                <  1:2818550/69‑1 (MQ=255)
                             aTCCAAACAATTGATGAAATTCAGTCCACCGAGACATTGATCGTCCTGCAAAACCCGATCATGCGTAcc                                <  1:134928/69‑1 (MQ=255)
                             aTCCAAACAATTGATGAAATTCAGTCCACCGAGACATTGATCGTCCTGCAAAACCCGATCATGCGTAcc                                <  1:2920076/69‑1 (MQ=255)
                              tCCAAACAATTGATGAAATTCAGTCCACCGAGACACTGATCGTCCTGCAGAATCCGATCATGCGTACCa                               <  1:3238179/69‑1 (MQ=255)
                              tCCAAACAATTGATGAAATTCAGTCCACCGAGACACTGATCGTCCTGCAGAATCCGATCATGCGTACCa                               <  1:2032471/69‑1 (MQ=255)
                              tCCAAACAATTGATGAAATTCAGTCCACCGAGACACTGATCGTCCTGCAGAATCCGATCATGCGTACCa                               <  1:2585449/69‑1 (MQ=255)
                              tCCAAACAATTGATGAAATTCAGTCCACCGAGACACTGATCGTCCTGCAGAATCCGATCATGCGTACCa                               <  1:2640753/69‑1 (MQ=255)
                              tCCAAACAATTGATGAAATTCAGTCCACCGAGACACTGATCGTCCTGCAGAATCCGATCATGCGTACCa                               <  1:730684/69‑1 (MQ=255)
                              tCCAAACAATTGATGAAATTCAGTCCACCGAGACACTGATCGTCCTGCAGAATCCGATCATGCGTACCa                               <  1:2123896/69‑1 (MQ=255)
                              tCCAAACAATTGATGAAATTCAGTCCACCGAGACACTGATCGTCCTGCAGAATCCGATCATGCGTACCa                               <  1:2147861/69‑1 (MQ=255)
                              tCCAAACAATTGATGAAATTCAGTCCACCGAGACACTGATCGTCCTGCAGAATCCGATCATGCGTACCa                               <  1:1287994/69‑1 (MQ=255)
                               ccAAACAATTGATGAAATTCAGTCCACCGAGACACTGATCGTCCTGCAGAATCCGATCATGCGTACCa                               <  1:8924/68‑1 (MQ=255)
                                   aCAATTGATGAAATTCAGTCCACCGAGACATTGATCGTCCTGCAAAACCCGATCATGCGTACCATCAAg                          <  1:1833521/69‑1 (MQ=255)
                                   aCAATTGATGAAATTCAGTCCACCGAGACATTGATCGTCCTGCAAAACCCGATCATGCGTACCATCAAg                          <  1:3301960/69‑1 (MQ=255)
                                   aCAATTGATGAAATTCAGTCCACCGAGACATTGATCGTCCTGCAAAACCCGATCATGCGTACCATCAAg                          <  1:387597/69‑1 (MQ=255)
                                   aCAATTGATGAAATTCAGTCCACCGAGACATTGATCGTCCTGCAAAACCCGATCATGCGTACCATCAAg                          <  1:2269808/69‑1 (MQ=255)
                                    cAATTGATGAAATTCAGTCCACCGAGACATTGATCGTCCTGCAAAACCCGAt                                          <  1:1152210/52‑1 (MQ=255)
                                        tgatgaAATTCAGTCCACCGAGACATTGATCGTCCTGCAAAACCCGATCATGCGTACCATCAAGCCCTg                     <  1:2280546/69‑1 (MQ=255)
                                        tgatgaAATTCAGTCCACCGAGACATTGATCGTCCTGCAAAACCCGATCATGCGTACCATCAAGCCCTg                     <  1:1377026/69‑1 (MQ=255)
                                        tgatgaAATTCAGTCCACCGAGACATTGATCGTCCTGCAAAACCCGATCATGCGTACCATCAAGCCCTg                     <  1:1640896/69‑1 (MQ=255)
                                        tgatgaAATTCAGTCCACCGAGACATTGATCGTCCTGCAAAACCCGATCATGCGTACCATCAAGCCCTg                     <  1:2346708/69‑1 (MQ=255)
                                            gaAATTCAGTCCACCGAGACATTGATCGTCCTGCAAAACCCGATCATGCGTACCATCAAGCCCTGATCg                 <  1:557331/69‑1 (MQ=255)
                                            gaAATTCAGTCCACCGAGACATTGATCGTCCTGCAAAACCCGATCATGCGTACCATCAAGCCCTGATCg                 <  1:940259/69‑1 (MQ=255)
                                            gaAATTCAGTCCACCGAGACATTGATCGTCCTGCAAAACCCGATCATGCGTACCATCAAGCCCTGATCg                 <  1:1663855/69‑1 (MQ=255)
                                                           gagaCATTGATCGTCCTGCAAAACCCGATCATGCGTACCATCAAGCCCTGATCGGCTTTTTTAATCCCa  <  1:2110803/69‑1 (MQ=255)
                                                           gagaCATTGATCGTCCTGCAAAACCCGATCATGCGTAACATCAAGCCCTGATCGGCTTTTTTAATCCCa  <  1:485071/69‑1 (MQ=255)
                                                            agaCATTGATCGTCCTGCAAAACCCGATCATGCGTACCATCAAGCCCTg                     <  1:3131205/49‑1 (MQ=255)
                                                                 |                                                              
ACGCTCTCCAGCATGTACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACATTGATCGTCCTGCAAAACCCGATCATGCGTACCATCAAGCCCTGATCGGCTTTTTTAATCCCA  >  minE/2459740‑2459867

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A