Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F1 I0 R1 4577 57.4 3436498 77.7% 2670158 63.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,464,777 G→T 53.2% A145A (GCG→GCT)  atpB → F0 sector of membrane‑bound ATP synthase, subunit a

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,464,7770GT53.2% ‑2.9 / 169.8 141A145A (GCG→GCT) atpBF0 sector of membrane‑bound ATP synthase, subunit a
Reads supporting (aligned to +/- strand):  ref base G (15/51);  new base T (25/50);  total (40/101)
Fisher's exact test for biased strand distribution p-value = 1.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01

Only 100 of 143 total aligned reads displayed.

TCGACCTGCTGCCGTACATTGCTGAACATGTACTGGGTCTGCCTGCACTGCGTGTGGTTCCGTCTGCGGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTATTTATCCTGATTCTGTTCTACAGCATCAA  >  minE/2464710‑2464842
                                                                   |                                                                 
tCGACCTGCTGCCGTACATTGCTGAACATGTACTGGGTCTGCCTGCACTGCGTGTGGTTCCGTCTGCgg                                                                  >  1:3257733/1‑69 (MQ=255)
tCGACCTGCTGCCGTACATTGCTGAACATGTACTGGGTCTGCCTGCACTGCGTGTGGTTCCGTCTGCg                                                                   >  1:1064944/1‑68 (MQ=255)
  gACCTGCTGCCGTACATTGCTGAACATGTACTGGGTCTGCCTGCACTGCGTGTGGTTCCGTCTGCGGAc                                                                >  1:353165/1‑69 (MQ=255)
  gACCTGCTGCCGTACATTGCTGAACATGTACTGGGTCTGCCTGCACTGCGTGTGGTTCCGTCTGCGGAc                                                                >  1:234453/1‑69 (MQ=255)
     ctgctgCCGTACATTGCTGAACATGTACTGGGTCTGCCTGCACTGCGTGTGGTTCCGTCTGCGGACGTg                                                             <  1:779092/69‑1 (MQ=255)
     ctgctgCCGTACATTGCTGAACATGTACTGGGTCTGCCTGCACTGCGTGTGGTTCCGTCTGCGGACGTg                                                             <  1:387471/69‑1 (MQ=255)
     ctgctgCCGTACATTGCTGAACATGTACTGGGTCTGCCTGCACTGCGTGTGGTTCCGTCTGCGGACGTg                                                             <  1:1759456/69‑1 (MQ=255)
     ctgctgCCGTACATTGCTGAACATGTACTGGGTCTGCCTGCACTGCGTGTGGTTCCGTCTGCGGACGTg                                                             <  1:224178/69‑1 (MQ=255)
     ctgctgCCGTACATTGCTGAACATGTACTGGGTCTGCCTGCACTGCGTGTGGTTCCGTCTGCGGACGTg                                                             <  1:1514104/69‑1 (MQ=255)
     ctgctgCCGTACATTGCTGAACATGTACTGGGTCTGCCTGCACTGCGTGTGGTTCCGTCTGCGGACGTg                                                             <  1:2857345/69‑1 (MQ=255)
             gTACATTGCTGAACATGTACTGGGTCTGCCTGCACTGCGTGTGGTTCCGTCTGCTGACGTGAACGTaa                                                      <  1:1756251/68‑1 (MQ=255)
             gTACATTGCTGAACATGTACTGGGTCTGCCTGCACTGCGTGTGGTTCCGTCTGCTGACGTGAACGTaa                                                      <  1:2365390/68‑1 (MQ=255)
             gTACATTGCTGAACATGTACTGGGTCTGCCTGCACTGCGTGTGGTTCCGTCTGCTGACGTGAACGTaa                                                      <  1:2445931/68‑1 (MQ=255)
             gTACATTGCTGAACATGTACTGGGTCTGCCTGCACTGCGTGTGGTTCCGTCTGCTGACGTGAACGTaa                                                      <  1:1409122/68‑1 (MQ=255)
             gTACATTGCTGAACATGTACTGGGTCTGCCTGCACTGCGTGTGGTTCCGTCTGCTGACGTGAACGTaa                                                      <  1:2917558/68‑1 (MQ=255)
             gTACATTGCTGAACATGTACTGGGTCTGCCTGCACTGCGTGTGGTTCCGTCTGCTGACGTGAACGTaa                                                      <  1:293296/68‑1 (MQ=255)
             gTACATTGCTGAACATGTACTGGGTCTGCCTGCACTGCGTGTGGTTCCGTCTGCTGACGTGAACGTaa                                                      <  1:1357666/68‑1 (MQ=255)
             gTACATTGCTGAACATGTACTGGGTCTGCCTGCACTGCGTGTGGTTCCGTCTGCTGACGTGAACGTaa                                                      <  1:113247/68‑1 (MQ=255)
             gTACATTGCTGAACATGTACTGGGTCTGCCTGCACTGCGTGTGGTTCCGTCTGCTGACGTGAACGTaa                                                      <  1:1157903/68‑1 (MQ=255)
              tACATTGCTGAACATGTACTGGGTCTGCCTGCACTGCGTGTGGTTCCGTCTGCTGACGTGAACGTaa                                                      <  1:2970134/67‑1 (MQ=255)
              tACATTGCTGAACATGTACTGGGTCTGCCTGCACTGCGTGTGGTTCCGTCTGCTGACGTGAACGTaa                                                      <  1:3380714/67‑1 (MQ=255)
              tACATTGCTGAACATGTACTGGGTCTGCCTGCACTGCGTGTGGTTCCGTCTGCTGACGTGAACGTaa                                                      <  1:3247106/67‑1 (MQ=255)
               aCATTGCTGAACATGTACTGGGTCTGCCTGCACTGCGTGTGGTTCCGTCTGCGGACGTGAACGTAACGc                                                   <  1:2685051/69‑1 (MQ=255)
               aCATTGCTGAACATGTACTGGGTCTGCCTGCACTGCGTGTGGTTCCGTCTGCGGACGTGAACGTAACGc                                                   <  1:240198/69‑1 (MQ=255)
               aCATTGCTGAACATGTACTGGGTCTGCCTGCACTGCGTGTGGTTCCGTCTGCGGACGTGAACGTAACGc                                                   <  1:3414515/69‑1 (MQ=255)
                   tGCTGAACATGTACTGGGTCTGCCTGCACTGCGTGTGGTTCCGTCTGCGGACGTGAACGTAACGCTGTc                                               <  1:1228290/69‑1 (MQ=255)
                    gCTGAACATGTACTGGGTCTGCCTGCACTGCGTGTGGTTCCGTCTGCGGACGTGAACGTAACGCTGTc                                               <  1:1503133/68‑1 (MQ=255)
                    gCTGAACATGTACTGGGTCTGCCTGCACTGCGTGTGGTTCCGTCTGCGGACGTGAACGTAACGCTGTc                                               <  1:1441355/68‑1 (MQ=255)
                       gAACATGTACTGGGTCTGCCTGCACTGCGTGTGGTTCCGTCTGCGGACGTGAACGTAACGCTGTCTATg                                           <  1:1443686/69‑1 (MQ=255)
                          cATGTACTGGGTCTGCCTGCACTGCGTGTGGTTCCGTCTGCGGACGTGAACGTAACGCTGTCTATGGc                                         <  1:2229543/68‑1 (MQ=255)
                          cATGTACTGGGTCTGCCTGCACTGCGTGTGGTTCCGTCTGCGGACGTGAACGTAACGCTGTCTATGGc                                         <  1:3242575/68‑1 (MQ=255)
                          cATGTACTGGGTCTGCCTGCACTGCGTGTGGTTCCGTCTGCGGACGTGAACGTAACGCTGTCTATGGc                                         <  1:972370/68‑1 (MQ=255)
                          cATGTACTGGGTCTGCCTGCACTGCGTGTGGTTCCGTCTGCGGACGTGAACGTAACGCTGTCTATGGc                                         <  1:108346/68‑1 (MQ=255)
                           aTGTACTGGGTCTGCCTGCACTGCGTGTGGTTCCGTCTGCGGACGTGAACGTAACGCTGTc                                               <  1:1379554/61‑1 (MQ=255)
                                    gTCTGCCTGCACTGCGTGTGGTTCCGTCTGCTGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTa                               >  1:1724494/1‑68 (MQ=255)
                                    gTCTGCCTGCACTGCGTGTGGTTCCGTCTGCTGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTa                               >  1:2728735/1‑68 (MQ=255)
                                    gTCTGCCTGCACTGCGTGTGGTTCCGTCTGCTGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTa                               >  1:2090457/1‑68 (MQ=255)
                                    gTCTGCCTGCACTGCGTGTGGTTCCGTCTGCTGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTa                               >  1:1324500/1‑68 (MQ=255)
                                    gTCTGCCTGCACTGCGTGTGGTTCCGTCTGCTGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTa                               >  1:3006451/1‑68 (MQ=255)
                                    gTCTGCCTGCACTGCGTGTGGTTCCGTCTGCTGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTa                               >  1:794675/1‑68 (MQ=255)
                                    gTCTGCCTGCACTGCGTGTGGTTCCGTCTGCTGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTa                               >  1:494429/1‑68 (MQ=255)
                                    gTCTGCCTGCACTGCGTGTGGTTCCGTCTGCTGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTa                               >  1:1121885/1‑68 (MQ=255)
                                    gTCTGCCTGCACTGCGTGTGGTTCCGTCTGCTGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTa                               >  1:490378/1‑68 (MQ=255)
                                     tCTGCCTGCACTGCGTGTGGTTCCGTCTGCTGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTa                               <  1:2798551/67‑1 (MQ=255)
                                     tCTGCCTGCACTGCGTGTGGTTCCGTCTGCTGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTa                               <  1:2673414/67‑1 (MQ=255)
                                     tCTGCCTGCACTGCGTGTGGTTCCGTCTGCTGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTa                               <  1:2849916/67‑1 (MQ=255)
                                     tCTGCCTGCACTGCGTGTGGTTCCGTCTGCTGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTa                               <  1:1398580/67‑1 (MQ=255)
                                     tCTGCCTGCACTGCGTGTGGTTCCGTCTGCTGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTa                               <  1:1286090/67‑1 (MQ=255)
                                     tCTGCCTGCACTGCGTGTGGTTCCGTCTGCTGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTa                               <  1:3362584/67‑1 (MQ=255)
                                     tCTGCCTGCACTGCGTGTGGTTCCGTCTGCTGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTa                               <  1:3281362/67‑1 (MQ=255)
                                     tCTGCCTGCACTGCGTGTGGTTCCGTCTGCTGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTa                               <  1:1369818/67‑1 (MQ=255)
                                     tCTGCCTGCACTGCGTGTGGTTCCGTCTGCTGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTa                               <  1:3051789/67‑1 (MQ=255)
                                     tCTGCCTGCACTGCGTGTGGTTCCGTCTGCTGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTa                               <  1:3251417/67‑1 (MQ=255)
                                     tCTGCCTGCACTGCGTGTGGTTCCGTCTGCTGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTa                               <  1:3191295/67‑1 (MQ=255)
                                     tCTGCCTGCACTGCGTGTGGTTCCGTCTGCTGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTa                               <  1:135690/67‑1 (MQ=255)
                                     tCTGCCTGCACTGCGTGTGGTTCCGTCTGCTGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTa                               <  1:339813/67‑1 (MQ=255)
                                     tCTGCCTGCACTGCGTGTGGTTCCGTCTGCTGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTa                               <  1:2714079/67‑1 (MQ=255)
                                     tCTGCCTGCACTGCGTGTGGTTCCGTCTGCTGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTa                               <  1:1263612/67‑1 (MQ=255)
                                     tCTGCCTGCACTGCGTGTGGTTCCGTCTGCTGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTa                               <  1:2636539/67‑1 (MQ=255)
                                     tCTGCCTGCACTGCGTGTGGTTCCGTCTGCTGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTa                               <  1:2507744/67‑1 (MQ=255)
                                     tCTGCCTGCACTGCGTGTGGTTCCGTCTGCTGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTa                               <  1:1589490/67‑1 (MQ=255)
                                     tCTGCCTGCACTGCGTGTGGTTCCGTCTGCTGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTa                               <  1:811166/67‑1 (MQ=255)
                                     tCTGCCTGCACTGCGTGTGGTTCCGTCTGCTGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTa                               <  1:2338158/67‑1 (MQ=255)
                                     tCTGCCTGCACTGCGTGTGGTTCCGTCTGCTGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTa                               <  1:841341/67‑1 (MQ=255)
                                     tCTGCCTGCACTGCGTGTGGTTCCGTCTGCTGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTa                               <  1:8610/67‑1 (MQ=255)
                                     tCTGCCTGCACTGCGTGTGGTTCCGTCTGCTGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTa                               <  1:877005/67‑1 (MQ=255)
                                     tCTGCCTGCACTGCGTGTGGTTCCGTCTGCTGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTa                               <  1:2082807/67‑1 (MQ=255)
                                     tCTGCCTGCACTGCGTGTGGTTCCGTCTGCTGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTa                               <  1:1893855/67‑1 (MQ=255)
                                     tCTGCCTGCACTGCGTGTGGTTCCGTCTGCGGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTAtt                             >  1:1026867/1‑69 (MQ=255)
                                     tCTGCCTGCACTGCGTGTGGTTCCGTCTGCGGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTAtt                             >  1:3209189/1‑69 (MQ=255)
                                     tCTGCCTGCACTGCGTGTGGTTCCGTCTGCGGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTAtt                             >  1:2147109/1‑69 (MQ=255)
                                     tCTGCCTGCACTGCGTGTGATTCCGTCTGCGGACGTGAAc                                                          >  1:1489472/1‑40 (MQ=255)
                                       tgcctgcACTGCGTGTGGTTCCGTCTGCGGACGTGAACGTAACGc                                                   <  1:1634245/45‑1 (MQ=255)
                                           tgcACTGTGTGTGGTTCCGTCTGCTGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTATTTATCCt                       >  1:1327993/1‑69 (MQ=255)
                                           tgcACTGTGTGTGGTTCCGTCTGCTGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTATTTATCCt                       >  1:3037378/1‑69 (MQ=255)
                                           tgcACTGTGTGTGGTTCCGTCTGCTGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTATTTATCCt                       >  1:921364/1‑69 (MQ=255)
                                           tgcACTGTGTGTGGTTCCGTCTGCTGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTATTTATCCt                       >  1:2885698/1‑69 (MQ=255)
                                           tgcACTGTGTGTGGTTCCGTCTGCTGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTATTTATCCt                       >  1:696513/1‑69 (MQ=255)
                                           tgcACTGTGTGTGGTTCCGTCTGCTGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTATTTATCCt                       >  1:629639/1‑69 (MQ=255)
                                           tgcACTGTGTGTGGTTCCGTCTGCTGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTATTTATCCt                       >  1:453189/1‑69 (MQ=255)
                                           tgcACTGTGTGTGGTTCCGTCTGCTGACGTGAACGTAACGCTGTCTATGGAACTGGGCGTATTTATCCt                       >  1:1590053/1‑69 (MQ=255)
                                                 gCGTGTGGTTCCGTCTGCGGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTATTTATCCTGATTCt                 <  1:1954295/69‑1 (MQ=255)
                                                 gCGTGTGGTTCCGTCTGCGGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTATTTATCCTGATTCt                 <  1:711964/69‑1 (MQ=255)
                                                 gCGTGTGGTTCCGTCTGCGGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTATTTATCCTGATTCt                 <  1:3125383/69‑1 (MQ=255)
                                                 gCGTGTGGTTCCGTCTGCGGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTATTTATCCTGATTCt                 <  1:2767602/69‑1 (MQ=255)
                                                 gCGTGTGGTTCCGTCTGCGGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTATTTATCCTGATTCt                 <  1:2546649/69‑1 (MQ=255)
                                                 gCGTGTGGTTCCGTCTGCGGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTATTTATCCTGATTCt                 <  1:369863/69‑1 (MQ=255)
                                                  cGTGTGGTTCCGTCTGCGGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTATTTATCCTGATTCt                 <  1:742568/68‑1 (MQ=255)
                                                        gTTCCGTCTGCGGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTATTTATCCTGATTCTg                <  1:45987/63‑1 (MQ=255)
                                                        gTTCCGTCTGCGGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTATTTATCCTGATTCTg                <  1:1557490/63‑1 (MQ=255)
                                                        gTTCCGTCTGCGGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTATTTATCCTGATTCTg                <  1:2381528/63‑1 (MQ=255)
                                                        gTTCCGTCTGCGGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTATTTATCCTGATTCTg                <  1:1547988/63‑1 (MQ=255)
                                                        gTTCCGTCTGCGGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTATTTATCCTGATTCTg                <  1:2928518/63‑1 (MQ=255)
                                                        gTTCCGTCTGCGGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTATTTATCCTGATTCTg                <  1:3295937/63‑1 (MQ=255)
                                                        gTTCCGTCTGCGGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTATTTATCCTGATTCTg                <  1:1372253/63‑1 (MQ=255)
                                                        gTTCCGTCTGCGGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTATTTATCCTGATTCTg                <  1:2538358/63‑1 (MQ=255)
                                                               cTGCGGACGTGAACGTAACGCTGTCTATGGCACTGGGCGt                                <  1:1688437/40‑1 (MQ=255)
                                                                tGCGGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTATTTATCCTGATTCTGTTCTACAGCATCaa  >  1:634568/1‑69 (MQ=255)
                                                                tGCGGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTATTTATCCTGATTCTGTTCTACAGCATCaa  >  1:1112329/1‑69 (MQ=255)
                                                                tGCGGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTATTTATCCTGATTCTGTTCTACAGCATCaa  >  1:521368/1‑69 (MQ=255)
                                                                   |                                                                 
TCGACCTGCTGCCGTACATTGCTGAACATGTACTGGGTCTGCCTGCACTGCGTGTGGTTCCGTCTGCGGACGTGAACGTAACGCTGTCTATGGCACTGGGCGTATTTATCCTGATTCTGTTCTACAGCATCAA  >  minE/2464710‑2464842

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A