Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,473,322 |
G→A |
42.1% |
P199P (CCG→CCA) |
glmS → |
L‑glutamine:D‑fructose‑6‑phosphate aminotransferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,473,322 | 0 | G | A | 42.1%
| 17.4
/ 40.6
| 57 | P199P (CCG→CCA) | glmS | L‑glutamine:D‑fructose‑6‑phosphate aminotransferase |
| Reads supporting (aligned to +/- strand): ref base G (8/25); new base A (4/20); total (12/45) |
| Fisher's exact test for biased strand distribution p-value = 5.33e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TGGTGATTGGCCTGGGGATGGGCGAAAACTTTATCGCTTCTGACCAGCTGGCGCTGTTGCCGGTGACCCGTCGCTTTATCTTCCTTGAAGAGGGCGATATTGCGGAAATCACTCGCCGTTCGGTAAA > minE/2473261‑2473387
|
tGGTGATTGGCCTGGGTATGGGCGAAAACTTTATCGCTTCTGACCAGCTGGCGCTGTTGCCAGTGActc > 1:589304/1‑67 (MQ=255)
tGGTGATTGGCCTGGGTATGGGCGAAAACTTTATCGCTTCTGACCAGCTGGCGCTGTTGCCAGTGActc > 1:3149557/1‑67 (MQ=255)
tGGTGATTGGCCTGGGTATGGGCGAAAACTTTATCGCTTCTGACCAGCTGGCGCTGTTGCCAGTGActc > 1:2992960/1‑67 (MQ=255)
tGGTGATTGGCCTGGGTATGGGCGAAAACTTTATCGCTTCTGACCAGCTGGCGCTGTTGCCAGTGActc > 1:1980993/1‑67 (MQ=255)
ggTGATTGGCCTGGGGATGGGCGAAAACTTTATCGCTTCTGACCAGCTGGCGCTGTTGCCGGTGACCCg > 1:694257/1‑69 (MQ=255)
ggTGATTGGCCTGGGGATGGGCGAAAACTTTATCGCTTCTGACCAGCTGGCGCTGTTGCCGGTGACCCg > 1:197130/1‑69 (MQ=255)
ggCCTGGGGATGGGCGAAAACTTTATCGCTTCTGACCAGCTGGCGCTGTTGCCGGTGACCCGTCGCttt < 1:827277/69‑1 (MQ=255)
ggCCTGGGGATGGGCGAAAACTTTATCGCTTCTGACCAGCTGGCGCTGTTGCCGGTGACCCGTCGCttt < 1:2711515/69‑1 (MQ=255)
ggCCTGGGGATGGGCGAAAACTTTATCGCTTCTGACCAGCTGGCGCTGTTGCCGGTGACCCGTCGCttt < 1:2513998/69‑1 (MQ=255)
ggCCTGGGGATGGGCGAAAACTTTATCGCTTCTGACCAGCTGGCGCTGTTGCCGGTGACCCGTCGCttt < 1:2017011/69‑1 (MQ=255)
ggCCTGGGGATGGGCGAAAACTTTATCGCTTCTGACCAGCTGGCGCTGTTGCCGGTGACCCGTCGCttt < 1:174985/69‑1 (MQ=255)
ggCGAAAACTTTATCGCTTCTGACCAGCTGGCGCTGTTGCCGGTGACCCGTCGCTTTATCTTCCTTGaa < 1:1060343/69‑1 (MQ=255)
ggCGAAAACTTTATCGCTTCTGACCAGCTGGCGCTGTTGCCGGTGACCCGTCGCTTTATCTTCCTTGaa < 1:895617/69‑1 (MQ=255)
ggCGAAAACTTTATCGCTTCTGACCAGCTGGCGCTGTTGCCGGTGACCCGTCGCTTTATCTTCCTTGaa < 1:2994743/69‑1 (MQ=255)
ggCGAAAACTTTATCGCTTCTGACCAGCTGGCGCTGTTGCCGGTGACCCGTCGCTTTATCTTCCTTGaa < 1:2894976/69‑1 (MQ=255)
cGAAAACTTTATCGCTTCTGACCAGCTGGCGCTGTTGCCGGTGACCCGTCGCTTTATCTTCCTTGAag < 1:954878/68‑1 (MQ=255)
cGAAAACTTTATCGCTTCTGACCAGCTGGCGCTGTTGCCGGTGACCCGTCGCTTTATCTTCCTTGAag < 1:913148/68‑1 (MQ=255)
cGAAAACTTTATCGCTTCTGACCAGCTGGCGCTGTTGCCGGTGACCCGTCGCTTTATCTTCCTTGAag < 1:3270564/68‑1 (MQ=255)
cGAAAACTTTATCGCTTCTGACCAGCTGGCGCTGTTGCCGGTGACCCGTCGCTTTATCTTCCTTGAag < 1:2180323/68‑1 (MQ=255)
cGAAAACTTTATCGCTTCTGACCAGCTGGCGCTGTTGCCGGTGACCCGTCGCTTTATCTTCCTTGAag < 1:1178405/68‑1 (MQ=255)
cGAAAACTTTATCGCTTCTGACCAGCTGGCGCTGTTGCCGGTGACCCGTCGCTTTATCTTCCTTGAag < 1:3093915/68‑1 (MQ=255)
aaCTTTATCGCTTCTGACCAGCTGGCGCTGTTGCCGGTGACCCGTCGCTTTATCTTCCTTGAAGAGGGc > 1:473081/1‑69 (MQ=255)
ttATCGCTTCTGACCAGCTGGCGCTGTTGCCGGTGACCCGTCGCTTTATCt > 1:1013143/1‑51 (MQ=255)
ttATCGCTTCTGACCAGCTGGCGCTGTTGCCGGTGACCCGTCGCTTTATCTTCCTTGAAGAGGGCGat > 1:2325363/1‑68 (MQ=255)
ttCTGACCAGCTGGCGCTGTTGCCGGTGACCCGTCGCTTTATCTTCCTTGAAGAGGGCGATATTGCTGa < 1:1305710/69‑1 (MQ=255)
ttCTGACCAGCTGGCGCTGTTGCCGGTGACCCGTCGCTTTATCTTCCTTGAAGAGGGCGATATTGCGGa < 1:1799706/69‑1 (MQ=255)
ttCTGACCAGCTGGCGCTGTTGCCGGTGACCCGTCGCTTTATCTTCCTTGAAGAGGGCGATATTGCGGa < 1:850359/69‑1 (MQ=255)
ttCTGACCAGCTGGCGCTGTTGCCGGTGACCCGTCGCTTTATCTTCCTTGAAGAGGGCGATATTGCGGa < 1:735563/69‑1 (MQ=255)
ttCTGACCAGCTGGCGCTGTTGCCGGTGACCCGTCGCTTTATCTTCCTTGAAGAGGGCGATATTGCGGa < 1:3147563/69‑1 (MQ=255)
ttCTGACCAGCTGGCGCTGTTGCCGGTGACCCGTCGCTTTATCTTCCTTGAAGAGGGCGATATTGCGGa < 1:3055566/69‑1 (MQ=255)
ttCTGACCAGCTGGCGCTGTTGCCGGTGACCCGTCGCTTTATCTTCCTTGAAGAGGGCGATATTGCGGa < 1:2081458/69‑1 (MQ=255)
ttCTGACCAGCTGGCGCTGTTGCCGGTGACCCGTCGCTTTATCTTCCTTGAAGAGGGCGATATTGCGGa < 1:2137782/69‑1 (MQ=255)
ttCTGACCAGCTGGCGCTGTTGCCGGTGACCCGGCGCTTTATCTTCCTTGAAGAGGGCGATATTGCGGa < 1:1629126/69‑1 (MQ=255)
gtccAGCTGGCGCTGTTGCCAGTGACTCGTCGTTTTATCTTCCTTGAAGAGGGCGATATTGCGGAAAt < 1:351404/66‑1 (MQ=255)
gACCAGCTGGCGCTGTTGCCAGTGACTCGTCGTTTTATCTTCCTTGAAGAGGGCTATATTGCGGAAAt < 1:3342961/68‑1 (MQ=255)
gACCAGCTGGCGCTGTTGCCAGTGACTCGTCGTTTTATCTTCCTTGAAGAGGGCGATATTGCGGAAAt < 1:96835/68‑1 (MQ=255)
gACCAGCTGGCGCTGTTGCCAGTGACTCGTCGTTTTATCTTCCTTGAAGAGGGCGATATTGCGGAAAt < 1:2757982/68‑1 (MQ=255)
gACCAGCTGGCGCTGTTGCCAGTGACTCGTCGTTTTATCTTCCTTGAAGAGGGCGATATTGCGGAAAt < 1:921628/68‑1 (MQ=255)
gACCAGCTGGCGCTGTTGCCAGTGACTCGTCGTTTTATCTTCCTTGAAGAGGGCGATATTGCGGAAAt < 1:2005128/68‑1 (MQ=255)
gACCAGCTGGCGCTGTTGCCAGTGACTCGTCGTTTTATCTTCCTTGAAGAGGGCGATATTGCGGAAAt < 1:1065610/68‑1 (MQ=255)
gACCAGCTGGCGCTGTTGCCAGTGACTCGTCGTTTTATCTTCCTTGAAGAGGGCGATATTGCGGAAAt < 1:749247/68‑1 (MQ=255)
gACCAGCTGGCGCTGTTGCCAGTGACTCGTCGTTTTATCTTCCTTGAAGAGGGCGATATTGCGGAAAt < 1:1090834/68‑1 (MQ=255)
gACCAGCTGGCGCTGTTGCCAGTGACTCGTCGTTTTATCTTCCTTGAAGAGGGCGATATTGCGGAAAt < 1:460925/68‑1 (MQ=255)
gACCAGCTGGCGCTGTTGCCAGTGACTCGTCGTTTTATCTTCCTTGAAGAGGGCGATATTGCGGAAAt < 1:3424931/68‑1 (MQ=255)
gACCAGCTGGCGCTGTTGCCAGTGACTCGTCGTTTTATCTTCCTTGAAGAGGGCGATATTGCGGAAAt < 1:2143396/68‑1 (MQ=255)
gACCAGCTGGCGCTGTTGCCAGTGACTCGTCGTTTTATCTTCCTTGAAGAGGGCGATATTGCGGAAAt < 1:3224163/68‑1 (MQ=255)
gACCAGCTGGCGCTGTTGCCAGTGACTCGTCGTTTTATCTTCCTTGAAGAGGGCGATATTGCGGAAAt < 1:2240800/68‑1 (MQ=255)
gACCAGCTGGCGCTGTTGCCAGTGACTCGTCGTTTTATCTTCCTTGAAGAGGGCGATATTGCGGAAAt < 1:1543691/68‑1 (MQ=255)
gACCAGCTGGCGCTGTTGCCAGTGACTCGTCGTTTTATCTTCCTTGAAGAGGGCGATATTGCGGAAAt < 1:1444273/68‑1 (MQ=255)
gACCAGCTGGCGCTGTTGCCAGTGACTCGTCGTTTTATCTTCCTTGAAGAGGGCGATATTGCGGAAAt < 1:1350524/68‑1 (MQ=255)
gACCAGCTGGCGCTGTTGCCAGTGACTCGTCGTTTTATCTTCCTTGAAGAGGGCGATATTGCGGAAAt < 1:1390042/68‑1 (MQ=255)
gACCAGCTGGCGCTGTTGCCAGTGAATCGTCGTTTTATCTTCCTTGAAGAGGGCGATATTGCGGAAAt < 1:1172987/68‑1 (MQ=255)
ttGCCAGTGACTCGTCGTTTTATCTTCCTTGAAGAGGGCGATATTGCGGAAAt < 1:1720573/53‑1 (MQ=255)
gCCGGTGACCCGTCGCTTTATCTTCCTTGAAGAGGGCGATATTGCgg > 1:1955627/1‑47 (MQ=255)
gCCGGTGACCCGTCGCTTTATCTTCCTTGAAGAGGGCGATATTGCGGa < 1:2432381/48‑1 (MQ=255)
gCCGGTGACCCGTCGCTTTATCTTCCTTGAAGAGGGCGATATTGCGGAAATCACTCGCCGTTCGGTaaa > 1:3397595/1‑69 (MQ=255)
gCCGGTGACCCGTCGCTTTATCTTCCTTGAAGAGGGCGATATTGCGGAAATCACTCGCCGTTCGGTaa > 1:2328873/1‑68 (MQ=255)
|
TGGTGATTGGCCTGGGGATGGGCGAAAACTTTATCGCTTCTGACCAGCTGGCGCTGTTGCCGGTGACCCGTCGCTTTATCTTCCTTGAAGAGGGCGATATTGCGGAAATCACTCGCCGTTCGGTAAA > minE/2473261‑2473387
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
