Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F17 I0 R2
|
278 |
43.2 |
2215644 |
94.6% |
2095999 |
61.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,473,718 |
C→T |
25.9% |
F331F (TTC→TTT) |
glmS → |
L‑glutamine:D‑fructose‑6‑phosphate aminotransferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,473,718 | 0 | C | T | 25.9%
| 97.8
/ 34.2
| 54 | F331F (TTC→TTT) | glmS | L‑glutamine:D‑fructose‑6‑phosphate aminotransferase |
| Reads supporting (aligned to +/- strand): ref base C (23/17); new base T (8/6); total (31/23) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GTTTCCCGCTACTGGTTTGAATCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTCCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGTCACAGTCTGGCGAAACCGCG > minE/2473653‑2473787
|
gTTTCCCGCTACTGGTTTGAATCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTa > 1:265157/1‑71 (MQ=255)
gTTTCCCGCTACTGGTTTGAATCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTa < 1:1821348/71‑1 (MQ=255)
tCCCGCTAATGGTTTGAATCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTCCGCTATCg > 1:634918/1‑71 (MQ=255)
ccGCTACTGGTTTGAATCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGc < 1:170253/70‑1 (MQ=255)
ccGCTACTGGTTTGAATCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTCCGCTATCGc > 1:1376055/1‑70 (MQ=255)
cGCTACTGGTTTGAATCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTCCGCTATCGCaa < 1:1958927/71‑1 (MQ=255)
cTGGTTTGAATCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTcc < 1:1298440/56‑1 (MQ=255)
ggTTTGAATCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTCCGCTATCGCaaa < 1:849720/65‑1 (MQ=255)
ggTTTGAATCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTCCGCTATCGCAAATCTGcc > 1:1695648/1‑71 (MQ=255)
gTTTGAATCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCg > 1:221005/1‑71 (MQ=255)
gTTTGAATCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTCCGCTAt > 1:2120803/1‑58 (MQ=255)
ttGAATCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTCCGCTATCGCAAATCTGCCGTg > 1:248361/1‑71 (MQ=255)
tGAATCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTCCGCTATCGCAAATCTGCCGTg < 1:1663180/70‑1 (MQ=255)
aaTCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTCCGCTATCGCaaa < 1:1890233/59‑1 (MQ=255)
aTCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTCCGCTATCGCAAATCTGCCGTGcgtc > 1:618196/1‑71 (MQ=255)
cGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTCCGCTATCGCAAATCTGCCGTGcgtcg > 1:1042841/1‑70 (MQ=255)
cGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTCCGCTATCGCAAATCTGCCGTGcgtcg > 1:145761/1‑70 (MQ=255)
gCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGCGTCGTa < 1:1288039/71‑1 (MQ=255)
gCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTCCGCTATCGCAAATCTGCCGTGcgtcgt < 1:316043/70‑1 (MQ=255)
gCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTCCGCTATCGCAAATCTGCCGTGCGTCGTAACa < 1:1789742/70‑1 (MQ=255)
ggTATTCCGTGCGACGTCGAAATCGCCTCTGAATTCCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCt < 1:1344888/71‑1 (MQ=255)
gTATTCCGTGCGACGTCGAAATCGCCTCTGAATTCCGc < 1:173402/38‑1 (MQ=255)
tATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGc > 1:425150/1‑56 (MQ=255)
ttCCGTGCCACGTCGAAATCGCCTCTGAATTCCGCTATCGCaa < 1:1514631/43‑1 (MQ=255)
ccGTGCGACGTCGAAATCGCCTCTGAATTCCGCTa > 1:197205/1‑35 (MQ=255)
ccGTGCGACGTCGAAATCGCCTCTGAATTCCGCTATCGCAAAt > 1:1836365/1‑43 (MQ=255)
ccGTGCGACGTCGAAATCGCCTCTGAATTCCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCtgatga > 1:512219/1‑70 (MQ=255)
gCGACGTCGAAATCGCCTCTGAATTCCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCa > 1:905600/1‑69 (MQ=255)
gACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTgatgat > 1:866475/1‑65 (MQ=255)
tCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCtgat < 1:2104701/58‑1 (MQ=255)
tCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCtgat < 1:775696/58‑1 (MQ=255)
gAAATCGCCTCTGAATTCCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGTcaca < 1:800716/71‑1 (MQ=255)
gAAATCGCCTCTGAATTCCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGTca > 1:531391/1‑69 (MQ=255)
gAAATCGCCTCTGAATTCCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGTca > 1:980424/1‑69 (MQ=255)
cGCCTCTGAATTCCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTgatgat > 1:32043/1‑54 (MQ=255)
cGCCTCTGAATTCCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGTCACAGTCTg > 1:1197213/1‑71 (MQ=255)
gCCTCTGAATTCCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGt < 1:234071/61‑1 (MQ=255)
gCCTCTGAATTCCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGt < 1:2069471/61‑1 (MQ=255)
gCCTCTGAATTCCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGt < 1:1501136/61‑1 (MQ=255)
gCCTCTGAATTCCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGt < 1:1867122/61‑1 (MQ=255)
gCCTCTGAATTCCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGt < 1:777865/61‑1 (MQ=255)
gCCTCTGAATTCCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGTCACAGTCTg < 1:455781/70‑1 (MQ=255)
ctctGAATTCCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGTca > 1:1875116/1‑61 (MQ=255)
ctctGAATTCCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGTca > 1:778080/1‑61 (MQ=255)
ctctGAATTCCGCTATAGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGTca > 1:552105/1‑61 (MQ=255)
ctGAATTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGTCACAGTCTGGCGa > 1:599468/1‑70 (MQ=255)
ctGAATTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGTCACAGTCTGGCGa > 1:65789/1‑70 (MQ=255)
ctGAATTTCGCTATCGCAAATCAGCCGTGCGTCGTAACAGCCTGATGATCACCTTGTCACAGTCTGGCg > 1:770892/1‑69 (MQ=255)
ctGAATTCCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGTCACAGTCTGGCGa > 1:2039083/1‑70 (MQ=255)
gAATTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGTCACAGTCTGGCGAAAc > 1:972656/1‑71 (MQ=255)
gAATTCCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGTCACAGTCTGGCGAAAc > 1:1673159/1‑71 (MQ=255)
gAATTCCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGTCACAGTCTGGCGAAAc > 1:1661473/1‑71 (MQ=255)
aaTTCCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGTc < 1:1645429/55‑1 (MQ=255)
aTTTCGCTATCGCAAATCTGCCTTGCGTCGTAACAGCCTGATGATCACCTTGTCACAGTCTGGCGAAAcc < 1:232499/70‑1 (MQ=255)
ttCCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGTcac > 1:764557/1‑55 (MQ=255)
ccGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGTCACAGTCTGGCGAAACcgcg < 1:697514/70‑1 (MQ=255)
ccGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGTCACAGTCTGGCGAAACcgcg < 1:336040/70‑1 (MQ=255)
|
GTTTCCCGCTACTGGTTTGAATCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTCCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGTCACAGTCTGGCGAAACCGCG > minE/2473653‑2473787
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
