Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,474,447 |
G→A |
31.9% |
P574P (CCG→CCA) |
glmS → |
L‑glutamine:D‑fructose‑6‑phosphate aminotransferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,474,447 | 0 | G | A | 31.9%
| 117.5
/ 66.4
| 131 | P574P (CCG→CCA) | glmS | L‑glutamine:D‑fructose‑6‑phosphate aminotransferase |
| Reads supporting (aligned to +/- strand): ref base G (63/26); new base A (30/12); total (93/38) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.19e-01 |
Only 100 of 138 total aligned reads displayed.
TTTGTAAGTAGCGATAACATGCACATCATCGAGATGCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCTTACCATGTCGCGCTGATCAAAGGCACCGACG > minE/2474385‑2474511
|
tttGTAAGTAGCGATAACATGCACATCATCGAGATGCCGCATGTGGAAGAGGTGATTGCACCGAtcttc < 1:2058402/69‑1 (MQ=255)
tttGTAAGTAGCGATAACATGCACATCATCGAGATGCCGCATGTGGAAGAGGTGATTGCACCGAtcttc < 1:2132992/69‑1 (MQ=255)
tttGTAAGTAGCGATAACATGCACATCATCGAGATGCCGCATGTGGAAGAGGTGATTGCACCGAtcttc < 1:3343264/69‑1 (MQ=255)
tttGTAAGTAGCGATAACATGCACATCATCGAGATGCCGCATGTGGAAGAGGTGATTGCACCGAtcttc < 1:2020501/69‑1 (MQ=255)
ttGTAAGTAGCGATAACATGCACATCATCGAGATGCCGCATGTGGAAGAGGTGATTGCACCg < 1:672084/62‑1 (MQ=255)
ttGTAAGTAGCGATAACATGCACATCATCGAGATGCCGCATGTGGAAGAGGTGATTGCACCg < 1:421010/62‑1 (MQ=255)
ttGTAAGTAGCGATAACATGCACATCATCGAGATGCCGCATGTGGAAGAGGTGATTGCACCg < 1:820036/62‑1 (MQ=255)
ttGTAAGTAGCGATAACATGCACATCATCGAGATGCCGCATGTGGAAGAGGTGATTGCACCg < 1:2955445/62‑1 (MQ=255)
ttGTAAGTAGCGATAACATGCACATCATCGAGATGCCGCATGTGGAAGAGGTGATTGCACCg < 1:135430/62‑1 (MQ=255)
gCGATAACATGCACATCATCGAGATGCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTc > 1:1142332/1‑69 (MQ=255)
gCGATAACATGCACATCATCGAGATGCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTc > 1:2781002/1‑69 (MQ=255)
gCGATAACATGCACATCATCGAGATGCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTc > 1:1944876/1‑69 (MQ=255)
tGCACATCATCGAGATGCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCa > 1:1057484/1‑67 (MQ=255)
tGCACATCATCGAGATGCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAgc > 1:812586/1‑69 (MQ=255)
tGCACATCATCGAGATGCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAgc > 1:635682/1‑69 (MQ=255)
tGCACATCATCGAGATGCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAgc > 1:3175942/1‑69 (MQ=255)
tGCACATCATCGAGATGCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAgc > 1:1059259/1‑69 (MQ=255)
tGCACATCATCGAGATGCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAgc > 1:2375039/1‑69 (MQ=255)
cacaTCATCGAGATGCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAgctg > 1:1776186/1‑69 (MQ=255)
cacaTCATCGAGATGCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAgctg > 1:1925997/1‑69 (MQ=255)
cacaTCATCGAGATGCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAgctg > 1:1529782/1‑69 (MQ=255)
cacaTCATCGAGATGCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAgctg > 1:1677906/1‑69 (MQ=255)
cacaTCATCGAGATGCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAgctg > 1:2579290/1‑69 (MQ=255)
cacaTCATCGAGATGCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAgctg > 1:925708/1‑69 (MQ=255)
cacaTCATCGAGATGCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAgctg > 1:2934127/1‑69 (MQ=255)
cacaTCATCGAGATGCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAgctg > 1:343929/1‑69 (MQ=255)
cacaTCATCGAGATGCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAgctg > 1:1338498/1‑69 (MQ=255)
cacaTCATCGAGATGCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAgctg > 1:969507/1‑69 (MQ=255)
cacaTCATCGAGATGCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAgctg > 1:3339681/1‑69 (MQ=255)
cacaTCATCGAGATGCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAgct > 1:1038286/1‑68 (MQ=255)
tcatcGAGATGCCGCATGTGGAAGAGGTTATTGCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTgg > 1:1768700/1‑69 (MQ=255)
atcGAGATGCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCt > 1:2040144/1‑69 (MQ=255)
atcGAGATGCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCt > 1:978065/1‑69 (MQ=255)
atcGAGATGCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCt > 1:576787/1‑69 (MQ=255)
atcGAGATGCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCt > 1:2525098/1‑69 (MQ=255)
atcGAGATGCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCt > 1:3104141/1‑69 (MQ=255)
atcGAGATGCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCt > 1:3203136/1‑69 (MQ=255)
atcGAGATGCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCt > 1:1355912/1‑69 (MQ=255)
atcGAGATGCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCt > 1:1549987/1‑69 (MQ=255)
atcGAGATGCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCt > 1:822839/1‑69 (MQ=255)
atcGAGATGCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAGCAGCTGGCt > 1:541948/1‑69 (MQ=255)
cGAGATGCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCTCTGCAGCTGCTGGCtt < 1:1233775/68‑1 (MQ=255)
cGAGATGCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCtt < 1:3265696/68‑1 (MQ=255)
cGAGATGCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCtt > 1:2816408/1‑68 (MQ=255)
cGAGATGCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCtt < 1:540319/68‑1 (MQ=255)
cGAGATGCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCtt < 1:1012889/68‑1 (MQ=255)
cGAGATGCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCtt < 1:681900/68‑1 (MQ=255)
cGAGATGCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCt > 1:3176485/1‑67 (MQ=255)
cGAGATGCCGCATGTGGAAGAGGTGATTGCACCAATCTTCTACACCGTTCCGCTGCTACTACTGGCtt < 1:2023679/68‑1 (MQ=255)
cGAGATGCCGCATGTGGAAGAGGTGATTGCACCAATCTTCTACACCGTTCCGCTGCAACTACTGGCtt < 1:1095687/68‑1 (MQ=255)
cGAGATGCCGCATGTGGAAGAGGTGATTGCACCAATCTTCTACACCGTTCCGCTGCAACTACTGGCtt > 1:1261618/1‑68 (MQ=255)
cGAGATGCCGCATGTGGAAGAGGTGATTGCACCAATCTTCTACACCGTTCCGCTGCAACTACTGGCtt > 1:761694/1‑68 (MQ=255)
cGAGATGCCGCATGTGGAAGAGGTGATTGCACCAATCTTCTACACCGTTCCGCTGCAACTACTGGCtt < 1:142144/68‑1 (MQ=255)
cGAGATGCCGCATGTGGAAGAGGTGATTGCACCAATCTTCTACACCGTTCCGCTGCAACTACTGGCtt > 1:1425749/1‑68 (MQ=255)
cGAGATGCCGCATGTGGAAGAGGTGATTGCACCAATCTTCTACACCGTTCCGCTGCAACTACTGGCtt > 1:289809/1‑68 (MQ=255)
cGAGATGCCGCATGTGGAAGAGGTGATTGCACCAATCTTCTACACCGTTCCGCTGCAACTACTGGCtt < 1:2856883/68‑1 (MQ=255)
cGAGATGCCGCATGTGGAAGAGGTGATTGCACCAATCTTCTACACCGTTCCGCTGCAACTACTGGCtt > 1:232748/1‑68 (MQ=255)
cGAGATGCCGCATGTGGAAGAGGTGATTGCACCAATCTTCTACACCGTTCCGCTGCAACTACTGGCtt > 1:483711/1‑68 (MQ=255)
cGAGATGCCGCATGTGGAAGAGGTGATTGCACCAATCTTCTACACCGTTCCGCTGCAACTACTGGCtt > 1:2131274/1‑68 (MQ=255)
cGAGATGCCGCATGTGGAAGAGGTGATTGCACCAATCTTCTACACCGTTCCGCTGCAACTACTGGCtt < 1:1062208/68‑1 (MQ=255)
cGAGATGCCGCATGTGGAAGAGGTGATTGCACCAATCTTCTACACCGTTCCGCTGCAACTACTGGCtt > 1:1777054/1‑68 (MQ=255)
cGAGATGCCGCATGTGGAAGAGGTGATTGCACCAATCTTCTACACCGTTCCGCTGCAACTACTGGCtt > 1:2226541/1‑68 (MQ=255)
cGAGATGCCGCATGTGGAAGAGGTGATTGCACCAATCTTCTACACCGTTCCGCTGCAACTACTGGCtt > 1:1640788/1‑68 (MQ=255)
cGAGATGCCGCATGTGGAAGAGGTGATTGCACCAATCTTCTACACCGTTCCGCTGCAACTACTGGCtt > 1:2512498/1‑68 (MQ=255)
cGAGATGCCGCATGTGGAAGAGGTGATTGCACCAATCTTCTACACCGTTCCGCTGCAACTACTGGCtt < 1:2500486/68‑1 (MQ=255)
cGAGATGCCGCATGTGGAAGAGGTGATTGCACCAATCTTCTACACCGTTCCGCTGCAACTACTGGCtt < 1:2474378/68‑1 (MQ=255)
cGAGATGCCGCATGTGGAAGAGGTGATTGCACCAATCTTCTACACCGTTCCGCTGCAACTACTGGCtt < 1:2244462/68‑1 (MQ=255)
cGAGATGCCGCATGTGGAAGAGGTGATTGCACCAATCTTCTACACCGTTCCGCTGCAACTACTGGCtt > 1:1707576/1‑68 (MQ=255)
cGAGATGCCGCATGTGGAAGAGGTGATTGCACCAATCTTCTACACCGTTCCGCTGCAACTACTGGCt > 1:796220/1‑67 (MQ=255)
gCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCTTACCATGt > 1:3128137/1‑69 (MQ=255)
gCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCTTACCATGt > 1:1608156/1‑69 (MQ=255)
gCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCTTACCATGt > 1:2435094/1‑69 (MQ=255)
gCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCTTACCATGt > 1:1689515/1‑69 (MQ=255)
cATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCTTACCATGTcgcg < 1:2467606/69‑1 (MQ=255)
cATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCTTACCATGTcgcg < 1:3147998/69‑1 (MQ=255)
cATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCTTACCATGTcgcg < 1:2984613/69‑1 (MQ=255)
cATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCTTACCATGTcgcg < 1:2818374/69‑1 (MQ=255)
cATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCTTACCATGTcgcg < 1:270725/69‑1 (MQ=255)
aTGTGGAAGAGGTGATTGCACCAATCTTCTACACCGTTCCGCTGCAACTACTGGCTTACCACGTtgcg > 1:204386/1‑68 (MQ=255)
aTGTGGAAGAGGTGATTGCACCAATCTTCTACACCGTTCCGCTGCAACTACTGGCTTACCACGTtgcg > 1:1202671/1‑68 (MQ=255)
aTGTGGAAGAGGTGATTGCACCAATCTTCTACACCGTTCCGCTGCAACTACTGGCTTACCACGTtgcg > 1:1569497/1‑68 (MQ=255)
aTGTGGAAGAGGTGATTGCACCAATCTTCTACACCGTTCCGCTGCAACTACTGGCTTACCACGTtgcg > 1:1839316/1‑68 (MQ=255)
aTGTGGAAGAGGTGATTGCACCAATCTTCTACACCGTTCCGCTGCAACTACTGGCTTACCACGTtgcg > 1:496218/1‑68 (MQ=255)
aTGTGGAAGAGGTGATTGCACCAATCTTCTACACCGTTCCGCTGCAACTACTGGCTTACCACGTtgcg > 1:3068456/1‑68 (MQ=255)
aTGTGGAAGAGGTGATTGCACCAATCTTCTACACCGTTCCGCTGCAACTACTGGCTTACCACGTtgc > 1:611489/1‑67 (MQ=255)
aTGTGGAAGAGGTGATTGCACCAATCTTCTACACCGTTCCGCTCCAactact > 1:857312/1‑52 (MQ=37)
gAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCTTACCATGTCGCGCTGAt > 1:713376/1‑68 (MQ=255)
gAAGAGGTGATTGCACCAATCTTCTACACCGTTCCGCTGCAACTACTGGCtt < 1:2251597/52‑1 (MQ=255)
aTTGCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCTTACCATGTCGCGCTGATCAAAGGCAcc < 1:3248055/69‑1 (MQ=255)
tGCCCCGATCTTCTACACCGTTCCGCTGCCGCTGCTGGCTTACCATGTCGCGCTGATCAAAGGCACCGa > 1:1713729/1‑69 (MQ=255)
tGCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCTTACCATGTCGCGCTGATCAAAGGCACCGa > 1:1854194/1‑69 (MQ=255)
tGCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCTTACCATGTCGCGCTGATCAAAGGCACCGa > 1:1614631/1‑69 (MQ=255)
tGCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCTAACCATGTCGCGCTGATCAAAGGCACCGa > 1:1921150/1‑69 (MQ=255)
gCACCGATCTTCTACACCTTTCCGCTGCAGCTGCTGGCTTACCATGTCGCGCTGATCAAAGGCACCGAc < 1:2844592/69‑1 (MQ=255)
gCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCTTACCATGTCGCGCTGATCAAAGGCACCGAc > 1:1028603/1‑69 (MQ=255)
gCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCTTACCATGTCGCGCTGATCAAAGGCACCGAc > 1:2122964/1‑69 (MQ=255)
gCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCTTACCATGTCGCGCTGATCAAAGGCACCGAc < 1:2424053/69‑1 (MQ=255)
aCCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCTTACCATGTCGCGCTGATCAAAgg > 1:935837/1‑60 (MQ=255)
aCCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCTTACCATGTCGCGCTGATCAAAGGCACCGACg > 1:1818720/1‑68 (MQ=255)
aCCGATCTTCTACACCGTTCCGCTGCAGCTGCTGCCTTACCATGTCGCGCTGATCAAAGGCACCGACg > 1:3395163/1‑68 (MQ=255)
|
TTTGTAAGTAGCGATAACATGCACATCATCGAGATGCCGCATGTGGAAGAGGTGATTGCACCGATCTTCTACACCGTTCCGCTGCAGCTGCTGGCTTACCATGTCGCGCTGATCAAAGGCACCGACG > minE/2474385‑2474511
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
