Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F28 I1 R1
|
122 |
92.7 |
4382561 |
94.3% |
4132755 |
66.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
minE |
2,473,718 |
C→T |
F331F (TTC→TTT) |
glmS → |
L‑glutamine:D‑fructose‑6‑phosphate aminotransferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,473,718 | 0 | C | T | 100.0%
| 185.7
/ NA
| 56 | F331F (TTC→TTT) | glmS | L‑glutamine:D‑fructose‑6‑phosphate aminotransferase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (30/26); total (30/26) |
GCTACTGGTTTGAATCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTCCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGTCACAGTCTGGCGAAACCG > minE/2473660‑2473785
|
gCTACTGGTTTGAATCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCaaa > 1:54387/1‑71 (MQ=255)
gCTACTGGTTTGAATCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCaaa > 1:2741355/1‑71 (MQ=255)
gCTACTGGTTTGAATCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCaaa > 1:2852966/1‑71 (MQ=255)
gCTACTGGTTTGAATCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCaaa > 1:2504805/1‑71 (MQ=255)
gCTACTGGTTTGAATCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCaaa > 1:2300193/1‑71 (MQ=255)
gCTACTGGTTTGAATCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCaaa > 1:3269511/1‑71 (MQ=255)
gCTACTGGTTTGAATCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCaaa > 1:1878471/1‑71 (MQ=255)
gCTACTGGTTTGAATCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCaaa > 1:403352/1‑71 (MQ=255)
ggTTTGAATCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGcc > 1:4055261/1‑71 (MQ=255)
ggTTTGAATCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGcc > 1:50873/1‑71 (MQ=255)
ggTTTGAATCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGcc > 1:1251349/1‑71 (MQ=255)
ggTTTGAATCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGcc > 1:2387317/1‑71 (MQ=255)
ggTTTGAATCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGcc > 1:2310873/1‑71 (MQ=255)
ggTTTGAATCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGcc > 1:45800/1‑71 (MQ=255)
ggTTTGAATCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGcc > 1:3341479/1‑71 (MQ=255)
ggTTTGAATCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGcc > 1:3510330/1‑71 (MQ=255)
ggTTTGAATCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGcc > 1:3735963/1‑71 (MQ=255)
ggTTTGAATCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGcc > 1:1474316/1‑71 (MQ=255)
gTTTGAATCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGc < 1:3451827/69‑1 (MQ=255)
gTTTGAATCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGc < 1:1497051/69‑1 (MQ=255)
aaTCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGcgt < 1:3513156/71‑1 (MQ=255)
aaTCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGcgt < 1:482648/71‑1 (MQ=255)
aaTCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGcgt < 1:4248343/71‑1 (MQ=255)
aaTCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGcgt < 1:2902739/71‑1 (MQ=255)
aaTCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGcgt < 1:2759602/71‑1 (MQ=255)
aaTCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGcgt < 1:109470/71‑1 (MQ=255)
aaTCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGcgt < 1:1080875/71‑1 (MQ=255)
aaTCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGcgt < 1:261793/71‑1 (MQ=255)
aaTCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGcgt < 1:249165/71‑1 (MQ=255)
aaTCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGcgt < 1:1443090/71‑1 (MQ=255)
aaTCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGcgt < 1:2286194/71‑1 (MQ=255)
aaTCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGcgt < 1:1619708/71‑1 (MQ=255)
aaTCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGcgt < 1:1771882/71‑1 (MQ=255)
aaTCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGcgt < 1:2660251/71‑1 (MQ=255)
aaTCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCAGTGcgt < 1:4301719/71‑1 (MQ=255)
aTCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGcgt < 1:3655225/70‑1 (MQ=255)
aTCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGcgt < 1:862103/70‑1 (MQ=255)
aTCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGcgt < 1:3294894/70‑1 (MQ=255)
aTCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGcgt < 1:2657826/70‑1 (MQ=255)
tATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCtga > 1:3473194/1‑71 (MQ=255)
tATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCtga > 1:2160443/1‑71 (MQ=255)
tATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCtga > 1:2135494/1‑71 (MQ=255)
tATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCtga > 1:2173536/1‑71 (MQ=255)
tATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCtga > 1:2027910/1‑71 (MQ=255)
tATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCtga > 1:3282435/1‑71 (MQ=255)
tATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCtga > 1:4099883/1‑71 (MQ=255)
tATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCtga > 1:830324/1‑71 (MQ=255)
tATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCtga > 1:2993448/1‑71 (MQ=255)
tATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCtga > 1:1412928/1‑71 (MQ=255)
tATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCtga > 1:1277378/1‑71 (MQ=255)
aaTTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGTCACAGTCTGGCg > 1:1103339/1‑66 (MQ=255)
aTTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGTCACAGTCTGGCGAAACcg < 1:2704931/71‑1 (MQ=255)
aTTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGTCACAGTCTGGCGAAACcg < 1:82241/71‑1 (MQ=255)
aTTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGTCACAGTCTGGCGAAACcg < 1:2516086/71‑1 (MQ=255)
aTTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGTCACAGTCTGGCGAAACcg < 1:4163137/71‑1 (MQ=255)
aTTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGTCACAGTCTGGCGAAACcg < 1:2187715/71‑1 (MQ=255)
|
GCTACTGGTTTGAATCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTCCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGTCACAGTCTGGCGAAACCG > minE/2473660‑2473785
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
