Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,507,398 |
T→G |
22.4% |
L154L (CTT→CTG) |
yicI → |
predicted alpha‑glucosidase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,507,398 | 0 | T | G | 22.4%
| 190.5
/ 58.8
| 125 | L154L (CTT→CTG) | yicI | predicted alpha‑glucosidase |
| Reads supporting (aligned to +/- strand): ref base T (69/28); new base G (9/19); total (78/47) |
| Fisher's exact test for biased strand distribution p-value = 3.03e-04 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.29e-01 |
Only 100 of 125 total aligned reads displayed.
CAGGTGAAAAATAATGGCTACGTGCAGGACACGAATAATCAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAGCGCTTTACTGCCCTGGTGCGCAATG > minE/2507333‑2507462
|
cAGGTGAAAAATAATGGCTACGTGCAGGACACGAATAATCAATGCAATTATATGTTTGAGCGGCTTGAt < 1:2416938/69‑1 (MQ=255)
cAGGTGAAAAATAATGGCTACGTGCAGGACACGAATAATCAACGCAATTATATGTTTGAGCGGCTTGa < 1:47890/68‑1 (MQ=255)
cAGGTGAAAAATAATGGCTACGTGCAGGACACGAATAATCAACGCAATTATATGTTTGAGCGGCTTGa < 1:3414201/68‑1 (MQ=255)
tGCAGGACACGAATAATCAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCTAAACAGtt < 1:1655893/68‑1 (MQ=255)
tGCAGGACACGAATAATCAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGtt < 1:3307989/68‑1 (MQ=255)
tGCAGGACACGAATAATCAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGtt < 1:1971967/68‑1 (MQ=255)
tGCAGGACACGAATAATCAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGtt < 1:3147649/68‑1 (MQ=255)
cacGAATAATGAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTc > 1:3096888/1‑68 (MQ=255)
cacGAATAATCAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCt > 1:1344042/1‑69 (MQ=255)
cacGAATAATCAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCt > 1:1698780/1‑69 (MQ=255)
cacGAATAATCAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCt > 1:2218771/1‑69 (MQ=255)
cacGAATAATCAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCt > 1:3113676/1‑69 (MQ=255)
cacGAATAATCAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCt > 1:873635/1‑69 (MQ=255)
cacGAATAATCAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCt > 1:3068512/1‑69 (MQ=255)
cacGAATAATCAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCt > 1:2508926/1‑69 (MQ=255)
cacGAATAATCAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCt > 1:2523930/1‑69 (MQ=255)
cacGAATAATCAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCt > 1:29154/1‑69 (MQ=255)
cacGAATAATCAACGAAATTATATGTTTGAGCGGCTGGATCTTGTCGTTGGCGAAACAGTTTACGGTCt > 1:2022340/1‑69 (MQ=255)
cacGAATAATCAACGAAATTATATGTTTGAGCGGCTGGATCTTGGCGTTGGCGAAACAGTTTACGGTCt > 1:2851061/1‑69 (MQ=255)
cacGAATAATCAACGAAATTATATGTTTGAGCGGCTGGATCTTGGCGTTGGCGAAACAGTTTACGGTCt > 1:2256249/1‑69 (MQ=255)
cacGAATAATCAACGAAATTATATGTTTGAGCGGCTGGATCTTGGCGTTGGCGAAACAGTTTACGGTCt > 1:248460/1‑69 (MQ=255)
cacGAATAATCAACGAAATTATATGTTTGAGCGGCTGGATCTTGGCGTTGGCGAAACAGTTTACGGTCt > 1:3022756/1‑69 (MQ=255)
cacGAATAATCAACGAAATTATATGTTTGAGCGGCTGGATCTTGGCGTTGGCGAAACAGTTTACGGTCt > 1:2916489/1‑69 (MQ=255)
cacGAATAATCAACGAAATTATATGTTTGAGCGGCTGGATCTTGGCGTTGGCGAAACAGTTTACGGTCt > 1:1137578/1‑69 (MQ=255)
cacGAATAATCAACGAAATTATATGTTTGAGCGGCTGGATCTTGGCGTTGGCGAAACAGTTTACGGTCt > 1:701757/1‑69 (MQ=255)
cacGAATAATCAACGAAATTATATGTTTGAGCGGCTGGATCTTGGCGTTGGCGAAACAGTTTACGGTCt > 1:740541/1‑69 (MQ=255)
cGAATAATCAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTgg < 1:1024548/69‑1 (MQ=255)
cGAATAATCAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTgg < 1:1689772/69‑1 (MQ=255)
cGAATAATCAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTgg < 1:2459118/69‑1 (MQ=255)
cGAATAATCAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTgg < 1:2405934/69‑1 (MQ=255)
cGAATAATCAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTgg < 1:1497778/69‑1 (MQ=255)
cGAATAATCAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTgg < 1:2287538/69‑1 (MQ=255)
atCAACGCAATTATATGTTTGAGCGGCTTGATCtt > 1:359295/1‑35 (MQ=255)
atCAACGCAATTATATGTTTGAGCGGCTTGATCtt > 1:2617891/1‑35 (MQ=255)
atCAACGCAATTATATGTTTGAGCGGCTTGATCtt > 1:1224251/1‑35 (MQ=255)
atCAACGCAATTATATGTTTGAGCGGCTTGATCtt > 1:95409/1‑35 (MQ=255)
atCAACGCAATTATATGTTTGAGCGGCTTGATCtt > 1:919806/1‑35 (MQ=255)
atCAACGCAATTATATGTTTGAGCGGCTTGATCtt > 1:1305435/1‑35 (MQ=255)
atCAACGCAATTATATGTTTGAGCGGCTTGATCtt > 1:1311844/1‑35 (MQ=255)
atCAACGCAATTATATGTTTGAGCGGCTTGATCtt > 1:2314269/1‑35 (MQ=255)
atCAACGCAATTATATGTTTGAGCGGCTTGATCtt > 1:3161220/1‑35 (MQ=255)
atCAACGCAATTATATGTTTGAGCGGCTTGATCtt > 1:3191142/1‑35 (MQ=255)
atCAACGCAATTATATGTTTGAGCGGCTTGATCtt > 1:3304985/1‑35 (MQ=255)
atCAACGCAATTATATGTTTGAGCGGCTTGATCtt > 1:2165112/1‑35 (MQ=255)
atCAACGCAATTATATGTTTGAGCGGCTTGATCtt > 1:2006309/1‑35 (MQ=255)
atCAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACTGTCTGGGAGAgc > 1:3347727/1‑69 (MQ=255)
tCAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAgcg > 1:1232426/1‑69 (MQ=255)
tCAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAgcg > 1:1699678/1‑69 (MQ=255)
tCAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAgcg > 1:2607325/1‑69 (MQ=255)
tCAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAgcg > 1:683572/1‑69 (MQ=255)
tCAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTAGGCTAAACAGTTTa > 1:979788/1‑54 (MQ=255)
cAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAgcgc > 1:726950/1‑69 (MQ=255)
cAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAgcgc > 1:721288/1‑69 (MQ=255)
cAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAgcgc > 1:588346/1‑69 (MQ=255)
cAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAgcgc > 1:1092243/1‑69 (MQ=255)
cAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAgcgc > 1:2181073/1‑69 (MQ=255)
cAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAgcgc > 1:1316207/1‑69 (MQ=255)
cAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAgcgc > 1:2792336/1‑69 (MQ=255)
cAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAgcgc > 1:111518/1‑69 (MQ=255)
cAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAgcgc > 1:1906576/1‑69 (MQ=255)
cAACGCAATAATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAgcgc > 1:2498680/1‑69 (MQ=255)
cGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAGCGCtt < 1:2901238/68‑1 (MQ=255)
cGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAGCGCtt < 1:1887439/68‑1 (MQ=255)
cGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAGCGCtt < 1:3117087/68‑1 (MQ=255)
cGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAGCGCtt < 1:283386/68‑1 (MQ=255)
gCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACATTTTACGGTCTGGGAGAGCTCtt < 1:2330424/67‑1 (MQ=255)
aTTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAAGAGtt < 1:1751263/44‑1 (MQ=255)
tataTGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACgg > 1:724179/1‑47 (MQ=255)
tataTGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACgg > 1:178607/1‑47 (MQ=255)
tataTGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAGCGCTTTACTGcc > 1:2467645/1‑69 (MQ=255)
tataTGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAGCGCTTTACTGcc > 1:2239818/1‑69 (MQ=255)
tataTGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAGCGCTTTACTGcc > 1:812027/1‑69 (MQ=255)
atGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAGCGCTTTACTGCCCt < 1:524698/68‑1 (MQ=255)
atGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAGCGCTTTACTGCCCt < 1:875054/68‑1 (MQ=255)
atGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAGCGCTTTACTGCCCt < 1:96648/68‑1 (MQ=255)
gTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAGCGCtt > 1:1492891/1‑57 (MQ=255)
gTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAGCGCtt > 1:3161485/1‑57 (MQ=255)
gTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAGCGCtt > 1:2220276/1‑57 (MQ=255)
gTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAGCGCtt > 1:3010550/1‑57 (MQ=255)
gTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAGCGCt > 1:1745728/1‑56 (MQ=255)
tttGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAGCGCTTTACTGCCCTGGTg > 1:1855232/1‑69 (MQ=255)
tttGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAGCGCTTTACTGCCCTGGTg > 1:293135/1‑69 (MQ=255)
tttGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAGCGCTTTAATGCCCTGGTg > 1:521300/1‑69 (MQ=255)
ttGAGCGGCTGGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAGCGCttt < 1:528572/56‑1 (MQ=255)
ttGAGCGGCTGGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAGCGCttt < 1:3056620/56‑1 (MQ=255)
ttGAGCGGCTGGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAGCGCttt < 1:1250929/56‑1 (MQ=255)
ttGAGCGGCTGGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAGCGCttt < 1:1299815/56‑1 (MQ=255)
ttGAGCGGCTGGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAGCGCttt < 1:2344030/56‑1 (MQ=255)
ttGAGCGGCTGGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAGCGCttt < 1:3178921/56‑1 (MQ=255)
ttGAGCGGCTGGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAGCGCttt < 1:3245127/56‑1 (MQ=255)
ttGAGCGGCTGGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAGCGCttt < 1:1496924/56‑1 (MQ=255)
ttGAGCGGCTGGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAGCGCttt < 1:1866407/56‑1 (MQ=255)
ttGAGCGGCTGGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAGCGCttt < 1:1674558/56‑1 (MQ=255)
ttGAGCGGCTGGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAGCGCttt < 1:710816/56‑1 (MQ=255)
ttGAGCGGCTGGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAGCGCttt < 1:2840034/56‑1 (MQ=255)
ttGAGCGGCTGGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAGCGCttt < 1:1787777/56‑1 (MQ=255)
ttGAGCGGCTGGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAGCGCttt < 1:623116/56‑1 (MQ=255)
ttGAGCGGCTGGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAGCGCttt < 1:490734/56‑1 (MQ=255)
tGAGCGGCTGGATCTTGGCGTTGGCGAAACAGTATACGGTCTGGGAGAGCGCttt < 1:989268/55‑1 (MQ=39)
aGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAGCGCtt < 1:2207454/52‑1 (MQ=255)
|
CAGGTGAAAAATAATGGCTACGTGCAGGACACGAATAATCAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAGCGCTTTACTGCCCTGGTGCGCAATG > minE/2507333‑2507462
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
