Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,508,978 |
T→C |
24.7% |
V681A (GTG→GCG) |
yicI → |
predicted alpha‑glucosidase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,508,978 | 0 | T | C | 24.7%
| 131.2
/ 39.6
| 109 | V681A (GTG→GCG) | yicI | predicted alpha‑glucosidase |
| Reads supporting (aligned to +/- strand): ref base T (66/16); new base C (16/11); total (82/27) |
| Fisher's exact test for biased strand distribution p-value = 3.89e-02 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.08e-01 |
Only 100 of 109 total aligned reads displayed.
TTTATGTGCGTGATAACACTCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCAAGACGGGCATGAAGCCGTCTGTGAAG > minE/2508918‑2509043
|
tttATGTGCGTGATAACACTCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGTGTGGCACg < 1:3223602/69‑1 (MQ=255)
cGTGATAACACTCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGTGTGGCACGAAGGCAc < 1:1483466/68‑1 (MQ=255)
gATAGCACCCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGCGTGGCACGAAGGTACGGCa > 1:615236/1‑69 (MQ=255)
gATAGCACCCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGCGTGGCACGAAGGTACGGCa > 1:2173725/1‑69 (MQ=255)
gATAGCACCCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGCGTGGCACGAAGGTACGGCa > 1:2204001/1‑69 (MQ=255)
gATAGCACCCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGCGTGGCACGAAGGTACGGCa < 1:2220210/69‑1 (MQ=255)
gATAGCACCCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGCGTGGCACGAAGGTACGGCa < 1:186877/69‑1 (MQ=255)
gATAGCACCCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGCGTGGCACGAAGGTACGGCa > 1:1822817/1‑69 (MQ=255)
gATAGCACCCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGCGTGGCACGAAGGTACGGCa < 1:484044/69‑1 (MQ=255)
gATAGCACCCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGCGTGGCACGAAGGTACGGCa > 1:481158/1‑69 (MQ=255)
gATAGCACCCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGCGTGGCACGAAGGTACGGCa > 1:2566665/1‑69 (MQ=255)
gATAGCACCCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGCGTGGCACGAAGGTACGGCa < 1:2614555/69‑1 (MQ=255)
gATAGCACCCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGCGTGGCACGAAGGTACGGCa > 1:1442416/1‑69 (MQ=255)
gATAGCACCCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGCGTGGCACGAAGGTACGGCa > 1:1352632/1‑69 (MQ=255)
gATAGCACCCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGCGTGGCACGAAGGTACGGCa > 1:1313341/1‑69 (MQ=255)
gATAGCACCCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGCGTGGCACGAAGGTACGGCa > 1:2821630/1‑69 (MQ=255)
gATAGCACCCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGCGTGGCACGAAGGTACGGCa < 1:1055499/69‑1 (MQ=255)
gATAGCACCCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGCGTGGCACGAAGGTACGGCa > 1:1235955/1‑69 (MQ=255)
gATAGCACCCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGCGTGGCACGAAGGTACGGCa < 1:418241/69‑1 (MQ=255)
gATAGCACCCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGCGTGGCACGAAGGTACGGCa < 1:1205169/69‑1 (MQ=255)
gATAGCACCCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGCGTGGCACGAAGGTACGGCa < 1:107203/69‑1 (MQ=255)
gATAGCACCCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGCGTGGCACGAAGGTACGGCa > 1:732050/1‑69 (MQ=255)
gATAGCACCCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGCGTGGCACGAAGGTACGGCa > 1:3069357/1‑69 (MQ=255)
gATAGCACCCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGCGTGGCACGAAGGTACGGCa > 1:1072849/1‑69 (MQ=255)
gATAGCACCCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGCGTGGCACGAAGGTACGGCa > 1:3198155/1‑69 (MQ=255)
gATAGCACCCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATAACGCGTGGCACGAAGGTACGGCa > 1:640421/1‑69 (MQ=255)
aTAGCACCCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGCGTGGCACGAAGGTACGGCa < 1:1182204/68‑1 (MQ=255)
aTAGCACCCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGCGTGGCACGAAGGTACGGCa < 1:2639065/68‑1 (MQ=255)
aTAACACTCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGTGTGGCACGAAGGCACGGc > 1:1623720/1‑67 (MQ=255)
acacTCTAGTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGTGTGGCACGAAGGCACGGCATTcc > 1:2318597/1‑69 (MQ=255)
acacTCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGTGTGGCACGAAGGCa > 1:3137831/1‑60 (MQ=255)
acacTCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGTGTGGCACGAAGGCACGGCATTcc > 1:2325762/1‑69 (MQ=255)
acacTCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGTGTGGCACGAAGGCACGGCATTcc > 1:3259043/1‑69 (MQ=255)
acacTCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGTGTGGCACGAAGGCACGGCATTcc > 1:1944094/1‑69 (MQ=255)
acacTCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGTGTGGCACGAAGGCACGGCATTcc > 1:740447/1‑69 (MQ=255)
acacTCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGTGTGGCACGAAGGCACGGCATTcc > 1:1101852/1‑69 (MQ=255)
acacTCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGTGTGGCACGAAGGCACGGCATTcc > 1:1571830/1‑69 (MQ=255)
acacTCTACTGGCGCTGGGAAACAACGATCAACGTCCCGATTACGTGTGGCACGAAGGCACGGCATTcc > 1:3148172/1‑69 (MQ=255)
cTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGTGTGGCACGAAGGCACGGCATTCCACCTCTTc > 1:2861873/1‑69 (MQ=255)
cTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGTGTGGCACGAAGGCACGGCATTCCACCTCTTc > 1:729121/1‑69 (MQ=255)
ggCGCTGGGCAACAACGATCAACGTCCCGATTACGTGTGGCACGAAGGCACGGCATTCCACCTCTTCaa < 1:3253813/69‑1 (MQ=255)
ggCGCTGGGCAACAACGATCAACGTCCCGATTACGTGTGGCACGAAGGCACGGCATTCCACCTCTTCaa < 1:2906974/69‑1 (MQ=255)
ggCGCTGGGCAACAACGATCAACGTCCCGATTACGTGTGGCACGAAGGCACGGCATTCCACCTCTTCaa < 1:1541148/69‑1 (MQ=255)
ggCGCTGGGCAACAACGATCAACGTCCCGATTACGTGTGGCACGAAGGCACGGCATTCCACCTCTTCaa < 1:2608217/69‑1 (MQ=255)
ggCGCTGGGCAACAACGATCAACGTCCCGATTACGTGTGGCACGAAGGCACGGCATTCCACCTCTTCaa < 1:3197011/69‑1 (MQ=255)
ggCAACAACGATCAACGTCCCGATTACGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCaa > 1:910802/1‑69 (MQ=255)
aacGATCAACGTCCCGATTACGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCAAGACggg > 1:1683683/1‑69 (MQ=255)
aacGATCAACGTCCCGATTACGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCAAGACggg > 1:2317808/1‑69 (MQ=255)
aacGATCAACGTCCCGATTACGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCAAGACggg > 1:416135/1‑69 (MQ=255)
aacGATCAACGTCCCGATTACGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCAAGACggg > 1:2576321/1‑69 (MQ=255)
aacGATCAACGTCCCGATTACGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCAAGACggg > 1:3244043/1‑69 (MQ=255)
aacGATCAACGTCCCGATTACGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCAAGACggg > 1:537769/1‑69 (MQ=255)
aacGATCAACGTCCCGATTACGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCAAGACggg > 1:1890866/1‑69 (MQ=255)
aacGATCAACGTCCCGATTACGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCAAGACggg > 1:2771416/1‑69 (MQ=255)
aacGATCAACGTCCCGATTACGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCAAGACggg > 1:2026996/1‑69 (MQ=255)
cAACGTCCCGATTACGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCAAGACGGGCATGaa > 1:1110720/1‑69 (MQ=255)
cAACGTCCCGATTACGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCAAGACGGGCATGaa > 1:3063920/1‑69 (MQ=255)
cAACGTCCCGATTACGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCAAGACGGGCATGaa > 1:387121/1‑69 (MQ=255)
cAACGTCCCGATTACGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCAAGACGGGCATGaa > 1:2107909/1‑69 (MQ=255)
cAACGTCCCGATTACGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCAAGACGGGCATGaa > 1:421870/1‑69 (MQ=255)
cAACGTCCCGATTACGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCAAGACGGGCATGaa > 1:129250/1‑69 (MQ=255)
cAACGTCCCGATTACGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCAAGACGGGCATGaa > 1:2686236/1‑69 (MQ=255)
cAACGTCCCGATTACGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCAAGACGGGCATGaa > 1:2574630/1‑69 (MQ=255)
cAACGTCCCGATTACGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCAAGACGGGCATGaa > 1:2504865/1‑69 (MQ=255)
cAACGTCCCGATTACGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCAAGACGGGCATGaa > 1:1673159/1‑69 (MQ=255)
cAACGTCCCGATTACGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCAAGACGGGCATGaa > 1:2455679/1‑69 (MQ=255)
cAACGTCCCGATTACGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCAAGACGGGCATGaa > 1:1753485/1‑69 (MQ=255)
cAACGTCCCGATTACGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCAAGACGGGCATGaa > 1:1862198/1‑69 (MQ=255)
cAACGTCCCGATTACGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCAAGACGGGCATGaa > 1:2306633/1‑69 (MQ=255)
cAACGTCCCGATTACGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCAAGACGGGCATGaa > 1:515431/1‑69 (MQ=255)
cAACGTCCCGATTACGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCAAGACGGGCATGaa > 1:2131566/1‑69 (MQ=255)
cAACGTACCGATTACGTGTGGCACGAAGGCACGGCATTCGACCTCTTCAATCTGCAAGACGGGCATGaa > 1:3264324/1‑69 (MQ=255)
ccGATTACGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCAAGACGGGCATGAAGc > 1:2280783/1‑64 (MQ=255)
ccGATTACGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCAAGACGGGCATGAAGCCGTCt > 1:927763/1‑69 (MQ=255)
ccGATTACGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCAAGACGGGCATGAAGCCGTCt > 1:1022797/1‑69 (MQ=255)
ccGATTACGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCAAGACGGGCATGAAGCCGTCt > 1:1474011/1‑69 (MQ=255)
ccGATTACGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCAAGACGGGCATGAAGCCGTCt > 1:216571/1‑69 (MQ=255)
aTTACGTGTGGCACGAAGGCACGGCATTCCACCTCTTCaa < 1:936500/40‑1 (MQ=255)
ttACGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCAAGACGGGCATGAAGCCGTCTGTGa < 1:353464/69‑1 (MQ=255)
ttACGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCAAGACGGGCATGAAGCCGTCTGTGa < 1:2470848/69‑1 (MQ=255)
tACGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCAAGACGGGCATg < 1:101775/55‑1 (MQ=255)
tACGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCAAGACGGGCATg < 1:3028824/55‑1 (MQ=255)
tACGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCAAGACGGGCATg < 1:887360/55‑1 (MQ=255)
tACGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCAAGACGGGCATg < 1:729830/55‑1 (MQ=255)
tACGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCAAGACGGGCATg < 1:2431797/55‑1 (MQ=255)
tACGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCAAGACGGGCATGAAGCCGTCTGTGaa > 1:1111395/1‑69 (MQ=255)
aCGTGTGGCACGAAGGCACGGCATTCCACCTCTTCa > 1:718543/1‑36 (MQ=255)
aCGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCAAGACggg > 1:2284997/1‑50 (MQ=255)
aCGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCAAGACggg > 1:1214567/1‑50 (MQ=255)
aCGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCAAGACggg > 1:132044/1‑50 (MQ=255)
aCGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCAAGACggg > 1:1502416/1‑50 (MQ=255)
aCGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCAAGACggg > 1:2275317/1‑50 (MQ=255)
aCGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCAAGACggg > 1:3064284/1‑50 (MQ=255)
aCGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCAAGACggg > 1:2952370/1‑50 (MQ=255)
aCGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCAAGACggg > 1:2987437/1‑50 (MQ=255)
aCGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCAAGACggg > 1:3421171/1‑50 (MQ=255)
aCGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCAAGACggg > 1:3317357/1‑50 (MQ=255)
aCGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCAAGACGGGCATGAAGCCGTCTGTGAAg > 1:2891393/1‑69 (MQ=255)
aCGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCAAGACGGGCATGAAGCCGTCTGTGAAg > 1:397247/1‑69 (MQ=255)
aCGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCAAGACGGGCATGAAGCCGTCTGTGAAg > 1:359224/1‑69 (MQ=255)
|
TTTATGTGCGTGATAACACTCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCAAGACGGGCATGAAGCCGTCTGTGAAG > minE/2508918‑2509043
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
