Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,530,970 |
G→T |
48.9% |
T216T (ACG→ACT) |
mutM → |
formamidopyrimidine/5‑formyluracil/ 5‑hydroxymethyluracil DNA glycosylase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,530,970 | 0 | G | T | 48.9%
| 15.3
/ 105.8
| 96 | T216T (ACG→ACT) | mutM | formamidopyrimidine/5‑formyluracil/ 5‑hydroxymethyluracil DNA glycosylase |
| Reads supporting (aligned to +/- strand): ref base G (23/26); new base T (15/32); total (38/58) |
| Fisher's exact test for biased strand distribution p-value = 1.49e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.86e-01 |
GTGTGAATTGTTAGCTCGGGTGATTAAAGCGGTGTTGCTGCGTTCGATTGAGCAGGGTGGTACAACGCTGAAAGATTTTCTGCAAAGTGATGGTAAACCGGGCTATTTCGCTCAGGAATTGCAGGTTTACGG > minE/2530904‑2531035
|
gtgtGAATTGTTAGCTCGGGTGATTAAAGCGGTGTTGCTGCGTTCGATTGAGCAGGGTGGTACAACGc > 1:2083713/1‑68 (MQ=255)
gtgtGAATTGTTAGCTCGGGTGATTAAAGCGGTGTTGCTGCGTTCGATTGAGCAGGGTGGTACAACGc > 1:1344232/1‑68 (MQ=255)
gtgtGAATTGTTAGCTCGGGTGATTAAAGCGGTGTTGCTGCGTTCGATTGAGCAGGGTGGTACAACGc > 1:1988767/1‑68 (MQ=255)
gtgtGAATTGTTAGCTCGGGTGATTAAAGCGGTGTTGCTGCGTTCGATTGAGCAGGGTGGTACAACGc > 1:254001/1‑68 (MQ=255)
gtgtGAATTGTTAGCTCGGGTGATTAAAGCGGTGTTGCTGCGTTCGATTGAGCAGGGTGGTACAACGc > 1:3059657/1‑68 (MQ=255)
gtgtGAATTGTTAGCTCGGGTGATTAAAGCGGTGTTGCTGCGTTCGATTGAGCAGGGTGGTACAACGc > 1:658900/1‑68 (MQ=255)
gtgtGAATTGTTAGCTCGGGTGATTAAAGCGGTGTTGCTGCGATCGATTGAGCAGGGTGGTACAACGc > 1:1583907/1‑68 (MQ=255)
aaTTGTTAGCTCGGGTGATTAAAGCGGTGTTGCTGCGTTCGATTGAGCAGGGTGGTACAACGCTGAAAg < 1:3377444/69‑1 (MQ=255)
aaTTGTTAGCTCGGGTGATTAAAGCGGTGTTGCTGCGTTCGATTGAGCAGGGTGGTACAACGCTGAAAg < 1:756111/69‑1 (MQ=255)
aaaGCGGTGTTGCTGCGTTCGATTGAGCAGGGTGGTACAACGCTGAAAGATTTTCTGCAAAGTGa < 1:2423779/65‑1 (MQ=255)
aaaGCGGTGTTGCTGCGTTCGATTGAGCAGGGTGGTACAACGCTGAAAGATTTTCTGCAAAGTGa < 1:231059/65‑1 (MQ=255)
aaaGCGGTGTTGCTGCGTTCGATTGAGCAGGGTGGTACAACGCTGAAAGATTTTCTGCAAAGTGa < 1:2105587/65‑1 (MQ=255)
aaaGCGGTGTTGCTGCGTTCGATTGAGCAGGGTGGTACAACGCTGAAAGATTTTCTGCAAAGTGa < 1:1887259/65‑1 (MQ=255)
aaaGCGGTGTTGCTGCGTTCGATTGAGCAGGGTGGTACAACGCTGAAAGATTTTCTGCAAAGTGa < 1:1576936/65‑1 (MQ=255)
gtTGCTGCGTTCGATTGAGCAGGGTGGTACAACGCTGAAAGATTTTCt < 1:1617788/48‑1 (MQ=255)
gtTGCTGCGTTCGATTGAGCAGGGTGGTACAACGCTGAAAGATTTTCt < 1:1316944/48‑1 (MQ=255)
cGTTCGATTGAGCAGGGTGGTACAACGCTGAAAGa > 1:999095/1‑35 (MQ=255)
cGTTCGATTGAGCAGGGTGGTACAACGCTGAAAGa > 1:1832174/1‑35 (MQ=255)
cGTTCGATTGAGCAGGGTGGTACAACGCTGAAAGa > 1:1547002/1‑35 (MQ=255)
cGTTCGATTGAGCAGGGTGGTACAACGCTGAAAGa > 1:1311891/1‑35 (MQ=255)
cGTTCGATTGAGCAGGGTGGTACAACGCTGAAAGa > 1:1343879/1‑35 (MQ=255)
cGTTCGATTGAGCAGGGTGGTACAACGCTGAAAGa > 1:1272727/1‑35 (MQ=255)
tCGATTGAGCAGGGTGGTACAACGCTGAAAGATTTTCTGCAAAGTGATGGTAAACCGGGCTATTTCGCt < 1:3322442/69‑1 (MQ=255)
ttGAGCAGGGTGGTACAACGCTGAAAGATTTTCTGCAAAGTGATGGTAAACCGGGCTATTTCGCTCAgg < 1:1244324/69‑1 (MQ=255)
ttGAGCAGGGTGGTACAACGCTGAAAGATTTTCTGCAAAGTGATGGTAAACCGGGCTATTTCGCTCAgg < 1:2723068/69‑1 (MQ=255)
ttGAGCAGGGTGGTACAACGCTGAAAGATTTTCTGCAAAGTGATGGTAAACCGGGCTATTTCGCTCAgg < 1:2719131/69‑1 (MQ=255)
ttGAGCAGGGTGGTACAACGCTGAAAGATTTTCTGCAAAGTGATGGTAAACCGGGCTATTTCGCTCAgg < 1:2573358/69‑1 (MQ=255)
ttGAGCAGGGTGGTACAACGCTGAAAGATTTTCTGCAAAGTGATGGTAAACCGGGCTATTTCGCTCAgg < 1:3106473/69‑1 (MQ=255)
ttGAGCAGGGTGGTACAACGCTGAAAGATTTTCTGCAAAGTGATGGTAAACCGGGCTATTTCGCTCAgg < 1:2628568/69‑1 (MQ=255)
ttGAGCAGGGTGGTACAACGCTGAAAGATTTTCTGCAAAGTGATGGTAAACCGGGCTATTTCGCTCAgg < 1:1711104/69‑1 (MQ=255)
tGAGCAGGGTGGTACAACGCTGAAAGATTTTCTGCAAAGTGATGGTAAACCTGGCTATTTCGCTCAGGa < 1:2428402/69‑1 (MQ=255)
tGAGCAGGGTGGTACAACGCTGAAAGATTTTCTGCAAAGTGATGGTAAACCGGGCTATTTCGCTCAGGa < 1:2908289/69‑1 (MQ=255)
tGAGCAGGGTGGTACAACGCTGAAAGATTTTCTGCAAAGTGATGGTAAACCGGGCTATTTCGCTCAGGa < 1:2608540/69‑1 (MQ=255)
tGAGCAGGGTGGTACAACGCTGAAAGATTTTCTGCAAAGTGATGGTAAACCGGGCTATTTCGCTCAGGa < 1:2678461/69‑1 (MQ=255)
tGAGCAGGGTGGTACAACGCTGAAAGATTTTCTGCAAAGTGATGGTAAACCGGGCTATTTCGCTCAGGa < 1:2903853/69‑1 (MQ=255)
aGGGTGGTACAACTCTGAAAGATTTTCTGCAAAGTGATgg > 1:61467/1‑40 (MQ=255)
aGGGTGGTACAACTCTGAAAGATTTTCTGCAAAGTGATg > 1:757066/1‑39 (MQ=255)
aGGGTGGTACAACTCTGAAAGATTTTCTGCAAAGTGATGGTAAACCGGGCTATTTCGCGCAGGAAt > 1:2513195/1‑66 (MQ=255)
aGGGTGGTACAACTCTGAAAGATTTTCTGCAAAGTGATGGTAAACCGGGCTATTTCGCGCAGGAAt > 1:2600378/1‑66 (MQ=255)
aGGGTGGTACAACTCTGAAAGATTTTCTGCAAAGTGATGGTAAACCGGGCTATTTCGCGCAGGAAt > 1:3232435/1‑66 (MQ=255)
aGGGTGGTACAACTCTGAAAGATTTTCTGCAAAGTGATGGTAAACCGGGCTATTTCGCGCAGGAAt > 1:1080721/1‑66 (MQ=255)
aGGGTGGTACAACTCTGAAAGATTTTCTGCAAAGTGATGGTAAACCGGGCTATTTCGCGCAGGAAt > 1:2864991/1‑66 (MQ=255)
aGGGTGGTACAACTCTGAAAGATTTTCTGCAAAGTGATGGTAAACCGGGCTATTTCGCGCAGGAAt > 1:176845/1‑66 (MQ=255)
aGGGTGGTACAACTCTGAAAGATTTTCTGCAAAGTGATGGTAAACCGGGCTATTTCGCGCAGGAAt > 1:1496096/1‑66 (MQ=255)
aGGGTGGTACAACTCTGAAAGATTTTCTGCAAAGTGATGGTAAACCGGGCTATTTCGCGCAGGAAt > 1:1520892/1‑66 (MQ=255)
aGGGTGGTACAACTCTGAAAGATTTTCTGCAAAGTGATGGTAAACCGGGCTATTTCGCGCAGGAAt > 1:1678569/1‑66 (MQ=255)
aGGGTGGTACAACTCTGAAAGATTTTCTGCAAAGTGATGGTAAACCGGGCTATTTCGCGCAGGAAt > 1:640029/1‑66 (MQ=255)
aGGGTGGTACAACTCTGAAAGATTTTCTGCAAAGTGATGGTAAACCGGGCTATTTCGCGCAGGAAt > 1:1771942/1‑66 (MQ=255)
aGGGTGGTACAACTCTGAAAGATTTTCTGCAAAGTGATGGTAAACCGGGCTATTTCGCGCAGGAAt > 1:556863/1‑66 (MQ=255)
aGGGTGGTACAACTCTGAAAGATTTTCTGCAAAGTGATGGTAAACCGGGCTATTTCGCGCAGGAAt > 1:1253285/1‑66 (MQ=255)
tggtACAACGCTGAAAGATTTTCTGCAAAGTGATGGTAAACCGGGCTATTTCGCTCAGGAATTGCAgg > 1:2637180/1‑68 (MQ=255)
gtACAACGCTGAAAGATTTTCTGCAAAGTGATGGTAAACCg > 1:1208607/1‑41 (MQ=255)
gtACAACGCTGAAAGATTTTCTGCAAAGTGATGGTAAACCGGGCTATTTCGCTCAGGAATTGCAGGttt > 1:1128159/1‑69 (MQ=255)
cAACTCTGAAAGATTTTCTGCAAAGTGATGGTAAACCgg < 1:2948372/39‑1 (MQ=255)
cAACTCTGAAAGATTTTCTGCAAAGTGATGGTAAACCgg < 1:289478/39‑1 (MQ=255)
cAACTCTGAAAGATTTTCTGCAAAGTGATGGTAAACCgg < 1:382763/39‑1 (MQ=255)
cAACTCTGAAAGATTTTCTGCAAAGTGATGGTAAACCgg < 1:956120/39‑1 (MQ=255)
cAACTCTGAAAGATTTTCTGCAAAGTGATGGTAAACCgg < 1:954002/39‑1 (MQ=255)
cAACTCTGAAAGATTTTCTGCAAAGTGATGGTAAACCgg < 1:849975/39‑1 (MQ=255)
cAACTCTGAAAGATTTTCTGCAAAGTGATGGTAAACCgg < 1:554481/39‑1 (MQ=255)
cAACTCTGAAAGATTTTCTGCAAAGTGATGGTAAACCgg < 1:1100859/39‑1 (MQ=255)
cAACTCTGAAAGATTTTCTGCAAAGTGATGGTAAACCgg < 1:1058302/39‑1 (MQ=255)
cAACTCTGAAAGATTTTCTGCAAAGTGATGGTAAACCgg < 1:1028915/39‑1 (MQ=255)
cAACTCTGAAAGATTTTCTGCAAAGTGATGGTAAACCgg < 1:3099339/39‑1 (MQ=255)
cAACTCTGAAAGATTTTCTGCAAAGTGATGGTAAACCgg < 1:3053457/39‑1 (MQ=255)
cAACTCTGAAAGATTTTCTGCAAAGTGATGGTAAACCgg < 1:585819/39‑1 (MQ=255)
cAACTCTGAAAGATTTTCTGCAAAGTGATGGTAAACCgg < 1:27013/39‑1 (MQ=255)
cAACTCTGAAAGATTTTCTGCAAAGTGATGGTAAACCgg < 1:1310792/39‑1 (MQ=255)
cAACTCTGAAAGATTTTCTGCAAAGTGATGGTAAACCgg < 1:15833/39‑1 (MQ=255)
cAACTCTGAAAGATTTTCTGCAAAGTGATGGTAAACCgg < 1:1732763/39‑1 (MQ=255)
cAACTCTGAAAGATTTTCTGCAAAGTGATGGTAAACCgg < 1:1738055/39‑1 (MQ=255)
cAACTCTGAAAGATTTTCTGCAAAGTGATGGTAAACCgg < 1:1807408/39‑1 (MQ=255)
cAACTCTGAAAGATTTTCTGCAAAGTGATGGTAAACCgg < 1:1253475/39‑1 (MQ=255)
cAACTCTGAAAGATTTTCTGCAAAGTGATGGTAAACCgg < 1:1883352/39‑1 (MQ=255)
cAACTCTGAAAGATTTTCTGCAAAGTGATGGTAAACCgg < 1:189307/39‑1 (MQ=255)
cAACTCTGAAAGATTTTCTGCAAAGTGATGGTAAACCgg < 1:1898386/39‑1 (MQ=255)
cAACTCTGAAAGATTTTCTGCAAAGTGATGGTAAACCgg < 1:2147867/39‑1 (MQ=255)
cAACTCTGAAAGATTTTCTGCAAAGTGATGGTAAACCgg < 1:1224161/39‑1 (MQ=255)
cAACTCTGAAAGATTTTCTGCAAAGTGATGGTAAACCgg < 1:1193331/39‑1 (MQ=255)
cAACTCTGAAAGATTTTCTGCAAAGTGATGGTAAACCgg < 1:2335497/39‑1 (MQ=255)
cAACTCTGAAAGATTTTCTGCAAAGTGATGGTAAACCgg < 1:2337897/39‑1 (MQ=255)
cAACTCTGAAAGATTTTCTGCAAAGTGATGGTAAACCgg < 1:2566433/39‑1 (MQ=255)
cAACTCTGAAAGATTTTCTGCAAAGTGATGGTAAACCgg < 1:277150/39‑1 (MQ=255)
cAACTCTGAAAGATTTTCTGCAAAGTGATGGTAAACCgg < 1:2632844/39‑1 (MQ=255)
cAACGCTGAAAGATTTTCTGCAAAGTGATGGTAAACCGGGCTATTTCGCTCAGGTATTGCAGGTTTACg < 1:1751000/69‑1 (MQ=255)
cAACGCTGAAAGATTTTCTGCAAAGTGATGGTAAACCGGGCTATTTCGCTCAGGAATTGCAGGTTTACg < 1:647989/69‑1 (MQ=255)
cAACGCTGAAAGATTTTCTGCAAAGTGATGGTAAACCGGGCTATTTCGCTCAGGAATTGCAGGTTTACg < 1:2675243/69‑1 (MQ=255)
aaCTCTGAAAGATTTTCTGCAAAGTGATGGTAAACCgg < 1:1258430/38‑1 (MQ=255)
aCGCTGAAAGATTTTCTGCAAAGTGATGGTAAACCGGGCTATTTCGCTCAGGAATTGCAGGtt > 1:1557815/1‑63 (MQ=255)
aCGCTGAAAGATTTTCTGCAAAGTGATGGTAAACCGGGCTATTTCGCTCAGGAATTGCAGGtt > 1:957235/1‑63 (MQ=255)
aCGCTGAAAGATTTTCTGCAAAGTGATGGTAAACCGGGCTATTTCGCTCAGGAATTGCAGGtt > 1:94167/1‑63 (MQ=255)
aCGCTGAAAGATTTTCTGCAAAGTGATGGTAAACCGGGCTATTTCGCTCAGGAATTGCAGGtt > 1:902962/1‑63 (MQ=255)
aCGCTGAAAGATTTTCTGCAAAGTGATGGTAAACCGGGCTATTTCGCTCAGGAATTGCAGGtt > 1:1036469/1‑63 (MQ=255)
aCGCTGAAAGATTTTCTGCAAAGTGATGGTAAACCGGGCTATTTCGCTCAGGAATTGCAGGtt > 1:549626/1‑63 (MQ=255)
aCGCTGAAAGATTTTCTGCAAAGTGATGGTAAACCGGGCTATTTCGCTCAGGAATTGCAGGTTTAcag > 1:715113/1‑66 (MQ=255)
aCGCTGAAAGATTATCTGCAAAGTGATGGTAAACCGGGCTATTTCGCt < 1:1155309/48‑1 (MQ=255)
|
GTGTGAATTGTTAGCTCGGGTGATTAAAGCGGTGTTGCTGCGTTCGATTGAGCAGGGTGGTACAACGCTGAAAGATTTTCTGCAAAGTGATGGTAAACCGGGCTATTTCGCTCAGGAATTGCAGGTTTACGG > minE/2530904‑2531035
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
