Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,620,337 |
T→A |
35.7% |
L134L (CTA→CTT) |
zntA ← |
zinc, cobalt and lead efflux system |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,620,337 | 0 | T | A | 35.7%
| 83.9
/ 77.1
| 95 | L134L (CTA→CTT) | zntA | zinc, cobalt and lead efflux system |
| Reads supporting (aligned to +/- strand): ref base T (47/14); new base A (19/15); total (66/29) |
| Fisher's exact test for biased strand distribution p-value = 3.86e-02 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.96e-01 |
AACGCCAGTTGCCCGAACGGATGATTGAACTGCTCCAGACCCCAGCTGATTGCCATCATCACGATTAGCGTAATCAGCGGCAGATTCTCTTTCAGGCGTGATGCTTGCGGTTCTTCGGCGGCCTGTTCA > minE/2620272‑2620400
|
aaCGCCAGTTGCCCGAACGGATGATTGAACTGCTCCAGCCCCCAGCTGATTGCCATCATCACGATTAg > 1:3355206/1‑68 (MQ=255)
aaCGCCAGTTGCCCGAACGGATGATTGAACTGCTCCAGACCCCAGCTGATTGCCATCATCACGATTa > 1:3287456/1‑67 (MQ=255)
aaCGCCAGTTGCCCGAACGGATGATTGAACTGCTCCAGACCCCAGCTGATTGCCATCATCACGATTAg > 1:2093967/1‑68 (MQ=255)
aaCGCCAGTTGCCCGAACGGATGATTGAACTGCTCCAGACCCCAGCTGATTGCCATCATCACGATTAg > 1:3168259/1‑68 (MQ=255)
aaCGCCAGTTGCCCGAACGGATGATTGAACTGCTCCAGACCCCAGCTGATTGCCATCATCACGATTAg > 1:3089738/1‑68 (MQ=255)
aaCGCCAGTTGCCCGAACGGATGATTGAACTGCTCCAGACCCCAGCTGATTGCCATCATCACGATTAg > 1:3000377/1‑68 (MQ=255)
aaCGCCAGTTGCCCGAACGGATGATTGAACTGCTCCAGACCCCAGCTGATTGCCATCATCACGATTAg > 1:3301573/1‑68 (MQ=255)
aaCGCCAGTTGCCCGAACGGATGATTGAACTGCTCCAGACCCCAGCTGATTGCCATCATCACGATTAg > 1:341299/1‑68 (MQ=255)
aaCGCCAGTTGCCCGAACGGATGATTGAACTGCTCCAGACCCCAGCTGATTGCCATCATCACGATTAg > 1:224054/1‑68 (MQ=255)
aaCGCCAGTTGCCCGAACGGATGATTGAACTGCTCCAGACCCCAGCTGATTGCCATCATCACGATTAg > 1:2058161/1‑68 (MQ=255)
aaCGCCAGTTGCCCGAACGGATGATTGAACTGCTCCAGACCCCAGCTGATTGCCATCATCACGATTAg > 1:528100/1‑68 (MQ=255)
aaCGCCAGTTGCCCGAACGGATGATTGAACTGCTCCAGACCCCAGCTGATTGCCATCATCACGATTAg > 1:2014849/1‑68 (MQ=255)
aaCGCCAGTTGCCCGAACGGATGATTGAACTGCTCCAGACCCCAGCTGATTGCCATCATCACGATTAg > 1:598314/1‑68 (MQ=255)
aaCGCCAGTTGCCCGAACGGATGATTGAACTGCTCCAGACCCCAGCTGATTGCCATCATCACGATTAg > 1:1587299/1‑68 (MQ=255)
aaCGCCAGTTGCCCGAACGGATGATTGAACTGCTCCAGACCCCAGCTGATTGCCATCATCACGATTAg > 1:1269234/1‑68 (MQ=255)
aaCGCCAGTTGCCCGAACGGATGATTGAACTGCTCAAGACCCCAGCTGATTGCCATCATCACGATTAg > 1:733713/1‑68 (MQ=255)
aaCGCCAGTTGCCCCAACGGATGATTGAACTGCTCCAGACCCCAGCTGATTGCCATCATCACGATTAg > 1:2252322/1‑68 (MQ=255)
cGCCAGTTGCCCGAACGGATGATTGAACTGCTCCAGACCCCAGCTGATTGCCATCATCACGATTAGCGt < 1:2081111/69‑1 (MQ=255)
gCCAGTTGCCCGAACGGATGATTGAACTGCTCCAGACCCCAGCTGATTGCCATCATCACGATTAGCGTa < 1:2414297/69‑1 (MQ=255)
gCCAGTTGCCCGAACGGATGATTGAACTGCTCCAGACCCCAGCTGATTGCCATCATCACGATTAGCGTa < 1:2368494/69‑1 (MQ=255)
gCCAGTTGCCCGAACGGATGATTGAACTGCTCCAGACCCCAGCTGATTGCCATCATCACGATTAGCGTa < 1:1004543/69‑1 (MQ=255)
gCCAGTTGCCCGAACGGATGATTGAACTGCTCCAGACCCCAGCTGATTGCCATCATCACGATTAGCGTa < 1:3005268/69‑1 (MQ=255)
gCCAGTTGCCCGAACGGATGATTGAACTGCTCCAGACCCCAGCTGATTGCCATCATCACGATTAGCGTa < 1:830944/69‑1 (MQ=255)
gCCCGAACGGATGATTGAACTGCTCCAGACCCCAGCTGATTGCCATCATCACGATTAGCGt > 1:2226627/1‑61 (MQ=255)
gCCCGAACGGATGATTGAACTGCTCCAGACCCCAGCTGATTGCCATCATCACGATTAGCGt > 1:2824499/1‑61 (MQ=255)
gCCCGAACGGATGATTGAACTGCTCCAGACCCCAGCTGATTGCCATCATCACGATTAGCGt > 1:1842910/1‑61 (MQ=255)
gCCCGAACGGATGATTGAACTGCTCCAGACCCCAGCTGATTGCCATCAACACGAtt > 1:1993048/1‑56 (MQ=255)
aaaCGGATGATTGAACTGCTCCAGCCCCCAACTGATTGCCATCATCACGATAAGCGTa < 1:3311995/57‑1 (MQ=255)
aaaCGGATGATTGAACTGCTCCAGCCCCCAACTGATTGCCATCATCACGATAAGCGTa < 1:2669697/57‑1 (MQ=255)
aaaCGGATGATTGAACTGCTCCAGCCCCCAACTGATTGCCATCATCACGATAAGCGTa < 1:2957655/57‑1 (MQ=255)
aaaCGGATGATTGAACTGCTCCAGCCCCCAACTGATTGCCATCATCACGATAAGCGTa < 1:1668761/57‑1 (MQ=255)
aaaCGGATGATTGAACTGCTCCAGCCCCCAACTGATTGCCATCATCACGATAAGCGTa < 1:174241/57‑1 (MQ=255)
aaaCGGATGATTGAACTGCTCCAGCCCCCAACTGATTGCCATCATCACGATAAGCGTa < 1:2707514/57‑1 (MQ=255)
aaaCGGATGATTGAACTGCTCCAGCCCCCAACTGATTGCCATCATCACGATAAGCGTa < 1:272143/57‑1 (MQ=255)
aaaCGGATGATTGAACTGCTCCAGCCCCCAACTGATTGCCATCATCACGATAAGCGTa < 1:2234014/57‑1 (MQ=255)
aaaCGGATGATTGAACTGCTCCAGCCCCCAACTGATTGCCATCATCACGATAAGCGTa < 1:1576992/57‑1 (MQ=255)
aaaCGGATGATTGAACTGCTCCAGCCCCCAACTGATTGCCATCATCACGATAAGCGTa < 1:1175781/57‑1 (MQ=255)
aaaCGGATGATTGAACTGCTCCAGCCCCCAACTGATTGCCATCATCACGATAAGCGTa < 1:2791877/57‑1 (MQ=255)
aaaCGGATGATTGAACTGCTCCAGCCCCCAACTGATTGCCATCATCACGATAAGCGTa < 1:1576279/57‑1 (MQ=255)
aaaCGGATGATTGAACTGCTCCAGCCCCCAACTGATTGCCATCATCACGATAAGCGTa < 1:1392888/57‑1 (MQ=255)
aaaCGGATGATTGAACTGCTCCAGCCCCCAACTGATTGCCATCATCACGATAAGCGTa < 1:908439/57‑1 (MQ=255)
aaaCGGATGATTGAACTCCTCCAGCCCCCAACTGATTGCCATCATCACGATAAGCGTa < 1:3266032/57‑1 (MQ=37)
cGGATGATTGAACTGCTCCAGACCCCAGCTGATTGCCATCATCACGATTAGCGTAATCa > 1:442350/1‑59 (MQ=255)
cGGATGATTGAACTGCTCCAGACCCCAGCTGATTGCCATCATCACGATTAGCGTAATCAGCGGCAGAtt > 1:843288/1‑69 (MQ=255)
cGGATGATTGAACTGCTCCAGACCCCAGCTGATTGCCATCATCACGATTAGCGTAATCAGCGGCAGAtt > 1:2081798/1‑69 (MQ=255)
cGGATGATTGAACTGCTCCAGACCCCAGCTGATTGCCATCATCACGATTAGCGTAATCAGCGGCAGAtt > 1:319026/1‑69 (MQ=255)
cGGATGATTGAACTGCTCCAGACCCCAGCTGATTGCCATCATCACGATTAGCGTAATCAGCGGCAGAt > 1:925739/1‑68 (MQ=255)
cGGATGATTGAACTGCTCCAGACCCCAGCTGATTGCCATCATCACGATTAGCGTAATCAGCGGCAGAt > 1:3381202/1‑68 (MQ=255)
tgatTGAACTGCTCCAGACCCCAGCTGATTGCCATCATCACGATTAGAGTAATCAGCGGCAGATtctc > 1:3355191/1‑68 (MQ=255)
atcaCGATTAGCGTAATCAGCGGCAGATTCTCTTTCAGGCGTGATGCTTGCGGTTCTTCtgcggc < 1:702755/65‑1 (MQ=255)
atcaCGATTAGCGTAATCAGCGGCAGATTCTCTTTCAGGCGTGATGCTTGCGGTTCTTCggcggc < 1:1244044/65‑1 (MQ=255)
atcaCGATTAGCGTAATCAGCGGCAGATTCTCTTTCAGGCGTGATGCTTGCGGTTCTTCggcggc < 1:1042423/65‑1 (MQ=255)
atcaCGATTAGCGTAATCAGCGGCAGATTCTCTTTCAGGCGTGATGCTTGCGGTTCTTCggcggc < 1:2800136/65‑1 (MQ=255)
atcaCGATTAGCGTAATCAGCGGCAGATTCTCTTTCAGGCGTGATGCTTGCGGTTCTTCggcggc < 1:1271170/65‑1 (MQ=255)
atcaCGATTAGCGTAATCAGCGGCAGATTCTCTTTCAGGCGTGATGCTTGCGGTTCTTCggcggc < 1:1325681/65‑1 (MQ=255)
atcaCGATTAGCGTAATCAGCGGCAGATTCTCTTTCAGGCGTGATGCTTGCGGTTCTTCggcggc < 1:1873377/65‑1 (MQ=255)
atcaCGATTAGCGTAATCAGCGGCAGATTCTCTTTCAGGCGTGATGCTTGCGGTTCTTCggcggc < 1:1624022/65‑1 (MQ=255)
atcaCGATTAGCGTAATCAGCGGCAGATTCTCTTTCAGGCGTGATGCTTGCGGTTCTTCGGCGGCCt > 1:3400343/1‑67 (MQ=255)
atcaCGATTAGCGTAATCAGCGGCAGATTCTCTTTCAGGCGTGATGCTTGCGGTTCTTCGGCGGCCTg > 1:3432237/1‑68 (MQ=255)
atcaCGATTAGCGTAATCAGCGGCAGATTCTCTTTCAGGCGTGATGCTTGCGGTTCTTCGGCGGCCTg > 1:429859/1‑68 (MQ=255)
atcaCGATTAGCGTAATCAGCGGCAGATTCTCTTTCAGGCGTGATGCTTGCGGTTCTTCGGCGGCCTg > 1:510822/1‑68 (MQ=255)
atcaCGATTAGCGTAATCAGCGGCAGATTCTCTTTCAGGCGTGATGCTTGCGGTTCTTCGGCGGCCTg > 1:998817/1‑68 (MQ=255)
atcaCGATTAGCGTAATCAGCGGCAGATTCTCTTTCAGGCGTGATGCTTGCGGTTCTTCGGCGGCCTg > 1:761222/1‑68 (MQ=255)
atcaCGATTAGCGTAATCAGCGGCAGATTCTCTTTCAGGCGTGATGCTTGCGGTTCTTCGGCGGCCTg > 1:3065266/1‑68 (MQ=255)
atcaCGATTAGCGTAATCAGCGGCAGATTCTCTTTCAGGCGTGATGCTTGCGGTTCTTCGGCGGCCTg > 1:139569/1‑68 (MQ=255)
atcaCGATTAGCGTAATCAGCGGCAGATTCTCTTTCAGGCGTGATGCTTGCGGTTCTTCGGCGGCCTg > 1:1749609/1‑68 (MQ=255)
atcaCGATTAGCGTAATCAGCGGCAGATTCTCTTTCAGGCGTGATGCTTGCGGTTCTTCGGCGGCCTg > 1:2320557/1‑68 (MQ=255)
atcaCGATTAGCGTAATCAGCGGCAGATTCTCTTTCAGGCGTGATGCTTGCGGTTCTTCGGCGGCCTg > 1:2323086/1‑68 (MQ=255)
atcaCGATTAGCGTAATCAGCGGCAGATTCTCTTTCAGGCGTGATGCTTGCGGTTCTTCGGCGGCCTg > 1:2449545/1‑68 (MQ=255)
atcaCGATTAGCGTAATCAGCGGCAGATTCTCTTTCAGGCGTGATGCTTGCGGTTCTTCGGCGGCCTg > 1:2853280/1‑68 (MQ=255)
atcaCGATTAGCGTAATCAGCGGCAGATTCTCTTTCAGGCGTGATGCTTGCGGTTCTTCGGCGGCCTg > 1:3114968/1‑68 (MQ=255)
atcaCGATTAGCGTAATCAGCGGCAGATTCTCTTTCAGGCGTGATGCTTGCGGTTCTTCGGCGGCCTg > 1:301191/1‑68 (MQ=255)
aCGATTAGCGTAATCAGCGGCAGATTCTCTTTCAGGCGTGATg > 1:1394485/1‑43 (MQ=255)
aCGATTAGCGTAATCAGCGGCAGATTCTCTTTCAGGCGTGATGCTTGCGGTTCTTCGGCGGCCTGTTCa > 1:2735764/1‑69 (MQ=255)
aCGATTAGCGTAATCAGCGGCAGATTCTCTTTCAGGCGTGATGCTTGCGGTTCTTCGGCGGCCTGTTCa > 1:1869448/1‑69 (MQ=255)
aCGATTAGCGTAATCAGCGGCAGATTCTCTTTCAGGCGTGATGCTTGCGGTTCTTCGGCGGCCTGTTCa > 1:588459/1‑69 (MQ=255)
aCGATTAGCGTAATCAGCGGCAGATTCTCTTTCAGGCGTGATGCTTGCGGTTCTTCGGCGGCATGTTCa > 1:2916101/1‑69 (MQ=255)
aCGATAAGCGTAATCAGCGGCAGATTCTCTTTCAGGCGTGATTCTTgtg > 1:1246175/1‑47 (MQ=38)
aCGATAAGCGTAATCAGCGGCAGATTCTCTTTCAGGCGTGATTCTTGTGGttcg > 1:2461339/1‑53 (MQ=255)
aCGATAAGCGTAATCAGCGGCAGATTCTCTTTCAGGCGTGATTCTTGTGGTTCGTCGGAGGCCTGTTCa > 1:711056/1‑69 (MQ=255)
aCGATAAGCGTAATCAGCGGCAGATTCTCTTTCAGGCGTGATTCTTGTGGTTCGTCGGAGGCCTGTTCa > 1:1110103/1‑69 (MQ=255)
aCGATAAGCGTAATCAGCGGCAGATTCTCTTTCAGGCGTGATTCTTGTGGTTCGTCGGAGGCCTGTTCa > 1:332859/1‑69 (MQ=255)
aCGATAAGCGTAATCAGCGGCAGATTCTCTTTCAGGCGTGATTCTTGTGGTTCGTCGGAGGCCTGTTCa > 1:890658/1‑69 (MQ=255)
aCGATAAGCGTAATCAGCGGCAGATTCTCTTTCAGGCGTGATTCTTGTGGTTCGTCGGAGGCCTGTTCa > 1:870076/1‑69 (MQ=255)
aCGATAAGCGTAATCAGCGGCAGATTCTCTTTCAGGCGTGATTCTTGTGGTTCGTCGGAGGCCTGTTCa > 1:1495998/1‑69 (MQ=255)
aCGATAAGCGTAATCAGCGGCAGATTCTCTTTCAGGCGTGATTCTTGTGGTTCGTCGGAGGCCTGTTCa > 1:3238426/1‑69 (MQ=255)
aCGATAAGCGTAATCAGCGGCAGATTCTCTTTCAGGCGTGATTCTTGTGGTTCGTCGGAGGCCTGTTCa > 1:1781794/1‑69 (MQ=255)
aCGATAAGCGTAATCAGCGGCAGATTCTCTTTCAGGCGTGATTCTTGTGGTTCGTCGGAGGCCTGTTCa > 1:371857/1‑69 (MQ=255)
aCGATAAGCGTAATCAGCGGCAGATTCTCTTTCAGGCGTGATTCTTGTGGTTCGTCGGAGGCCTGTTCa > 1:2481014/1‑69 (MQ=255)
aCGATAAGCGTAATCAGCGGCAGATTCTCTTTCAGGCGTGATTCTTGTGGTTCGTCGGAGGCCTGTTCa > 1:1970568/1‑69 (MQ=255)
aCGATAAGCGTAATCAGCGGCAGATTCTCTTTCAGGCGTGATTCTTGTGGTTCGTCGGAGGCCTGTTCa > 1:2033795/1‑69 (MQ=255)
aCGATAAGCGTAATCAGCGGCAGATTCTCTTTCAGGCGTGATTCTTGTGGTTCGTCGGAGGCCTGTTCa > 1:515081/1‑69 (MQ=255)
aCGATAAGCGTAATCAGCGGCAGATTCTCTTTCAGGCGTGATTCTTGTGGTTCGTCGGAGGCCTGTTCa > 1:2207966/1‑69 (MQ=255)
aCGATAAGCGTAATCAGCGGCAGATTCTCTTTCAGGCGTGATTCTTGTGGTTCGTCGGAGGCCTGTTCa > 1:2948386/1‑69 (MQ=255)
aCGATAAGCGTAATCAGCGGCAGATTCTCTTTCAGGCGTGATTCTTGTGGTTCGTAggaggc > 1:2705350/1‑62 (MQ=255)
aCGATAAGCGTAATCAGAGGCAGATTCTCTTTCAGGCGTGATTCTTGTGGTTCGTCGGAGGCCTGTTCa > 1:3010782/1‑69 (MQ=255)
|
AACGCCAGTTGCCCGAACGGATGATTGAACTGCTCCAGACCCCAGCTGATTGCCATCATCACGATTAGCGTAATCAGCGGCAGATTCTCTTTCAGGCGTGATGCTTGCGGTTCTTCGGCGGCCTGTTCA > minE/2620272‑2620400
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
