Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,624,178 |
G→T |
47.2% |
A411A (GCG→GCT) |
ftsY → |
fused Signal Recognition Particle (SRP) receptor |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,624,178 | 0 | G | T | 47.2%
| 21.5
/ 73.0
| 74 | A411A (GCG→GCT) | ftsY | fused Signal Recognition Particle (SRP) receptor |
| Reads supporting (aligned to +/- strand): ref base G (9/30); new base T (10/25); total (19/55) |
| Fisher's exact test for biased strand distribution p-value = 6.06e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01 |
CAAATCGCACCTGATGGAAGAGTTGAAGAAAATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCGCCGCATGAAGTTATGCTGACTATTGATGCCAGCACCGGGCAGAACGCGGTAAGCCAGGCCAAA > minE/2624112‑2624241
|
cAAATCGCACCTGATGGAAGAGTTGAAGAAAATCGTCCGCGTGATGAAGAAACTCGACGTTGAAgcgc > 1:1012086/1‑68 (MQ=255)
cAAATCGCACCTGATGGAAGAGTTGAAGAAAATCGTCCGCGTGATGAAGAAACTCGACGTTGAAgcgc > 1:2362337/1‑68 (MQ=255)
cAAATCGCACCTGATGGAAGAGTTGAAGAAAATCGTCCGCGTGATGAAGAAACTCGACGTTGAAgcgc > 1:2166325/1‑68 (MQ=255)
cAAATCGCACCTGATGGAAGAGTTGAAGAAAATCGTCCGCGTGATGAAGAAACTCGACGTTGAAgcgc > 1:1616478/1‑68 (MQ=255)
cAAATCGCACCTGATGGAAGAGTTGAAGAAAATCGTCCGCGTGATGAAGAAACTCGACGTTGAAgcgc > 1:1409643/1‑68 (MQ=255)
cAAATCGCACCTGATGGAAGAGTTGAAGAAAATCGTCCGCGTGATGAAGAAACTCGACGTTGAAgcgc > 1:934424/1‑68 (MQ=255)
cAAATCGCACCTGATGGAAGAGTTGAAGAAAATCGTCCGCGTGATGAAGAAACTCGACGTTGAAgcgc > 1:973896/1‑68 (MQ=255)
cAAATCGCACCTGATGGAAGAGTTGAAGAAAATCGTCCGCGTGATGAAGAAACTCGACGTTGAAgcgc > 1:935385/1‑68 (MQ=255)
ccTGATGGAAGAGTTGAAGAAAATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCGCCGCATGaatt < 1:3159177/69‑3 (MQ=255)
ccTGATGGAAGAGTTGAAGAAAATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCGCCGCATGAAGt < 1:2381798/69‑1 (MQ=255)
tGATGGAAGAGTTGAAGAAAATCGTCCGCGTGATGAATAAACTCGACGTTGAAGCGCCGCATGAAGTTa < 1:1892437/69‑1 (MQ=255)
tGATGGAAGAGTTGAAGAAAATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCGCCGCATGAAGTTa < 1:475861/69‑1 (MQ=255)
aTGGAAGAGTTGAAGAAAATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCGCCGCATGAAGt < 1:1119124/65‑1 (MQ=255)
ggAAGAGTTGAAGAAAATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCGCCGCATGAAGTTATGCt < 1:2662333/69‑1 (MQ=255)
gAAGAGTTGAAGAAAATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCGCCGCATGAAGTTATGCTg > 1:2397924/1‑69 (MQ=255)
agagTTGAAGAAAATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCGCCGCATGAAGTTATGCt < 1:2198144/66‑1 (MQ=255)
agagTTGAAGAAAATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCGCCGCATGAAGTTATGCt < 1:2249454/66‑1 (MQ=255)
agagTTGAAGAAAATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCGCCGCATGAAGTTATGCt < 1:2598778/66‑1 (MQ=255)
agagTTGAAGAAAATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCGCCGCATGAAGTTATGCt < 1:2951572/66‑1 (MQ=255)
agagTTGAAGAAAATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCGCCGCATGAAGTTATGCTGAc < 1:979257/69‑1 (MQ=255)
agagTTGAAGAAAATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCGCCGCATGAAGTTATGCTGAc < 1:626748/69‑1 (MQ=255)
agagTTGAAGAAAATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCGCCGCATGAAGTTATGCTGAc < 1:2770874/69‑1 (MQ=255)
gTTGAAGAAAATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCTCCGCATGAAGTTATGCTGACTAt < 1:100819/69‑1 (MQ=255)
gTTGAAGAAAATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCTCCGCATGAAGTTATGCTGACTAt < 1:984780/69‑1 (MQ=255)
gTTGAAGAAAATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCTCCGCATGAAGTTATGCTGACTAt < 1:281797/69‑1 (MQ=255)
gTTGAAGAAAATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCTCCGCATGAAGTTATGCTGACTAt < 1:300509/69‑1 (MQ=255)
gTTGAAGAAAATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCTCCGCATGAAGTTATGCTGACTAt < 1:3072883/69‑1 (MQ=255)
gTTGAAGAAAATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCTCCGCATGAAGTTATGCTGACTAt < 1:3076895/69‑1 (MQ=255)
gTTGAAGAAAATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCTCCGCATGAAGTTATGCTGACTAt < 1:3244936/69‑1 (MQ=255)
gTTGAAGAAAATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCTCCGCATGAAGTTATGCTGACTAt < 1:3428901/69‑1 (MQ=255)
gTTGAAGAAAATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCTCCGCATGAAGTTATGCTGACTAt < 1:387526/69‑1 (MQ=255)
gTTGAAGAAAATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCTCCGCATGAAGTTATGCTGACTAt < 1:41331/69‑1 (MQ=255)
gTTGAAGAAAATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCTCCGCATGAAGTTATGCTGACTAt < 1:547849/69‑1 (MQ=255)
gTTGAAGAAAATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCTCCGCATGAAGTTATGCTGACTAt < 1:907263/69‑1 (MQ=255)
gTTGAAGAAAATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCTCCGCATGAAGTTATGCTGACTAt < 1:1631048/69‑1 (MQ=255)
gTTGAAGAAAATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCTCCGCATGAAGTTATGCTGACTAt < 1:1880986/69‑1 (MQ=255)
gTTGAAGAAAATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCTCCGCATGAAGTTATGCTGACTAt < 1:1811203/69‑1 (MQ=255)
gTTGAAGAAAATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCTCCGCATGAAGTTATGCTGACTAt < 1:1179361/69‑1 (MQ=255)
gTTGAAGAAAATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCTCCGCATGAAGTTATGCTGACTAt < 1:1196557/69‑1 (MQ=255)
gTTGAAGAAAATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCTCCGCATGAAGTTATGCTGACTAt < 1:2129340/69‑1 (MQ=255)
gTTGAAGAAAATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCTCCGCATGAAGTTATGCTGACTAt < 1:1062063/69‑1 (MQ=255)
gTTGAAGAAAATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCTCCGCATGAAGTTATGCTGACTAt < 1:2301214/69‑1 (MQ=255)
ttGAAGAAAATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCTCCGCATGAAGTTATGCTGACTAt < 1:2634572/68‑1 (MQ=255)
ttGAAGAAAATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCGCCGCATGAAGTTATGCTGACTAtt < 1:91716/69‑1 (MQ=255)
ttGAAGAAAATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCGCCGCATGAAGTTATGCTGACTAtt < 1:1832368/69‑1 (MQ=255)
tGAAGAAAATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCGCCGCATGAAGTTATGCTGACTAtt < 1:1460569/68‑1 (MQ=255)
gaagaaAATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCGCCGCATGAAGTTATGCTGACTAtt < 1:1329390/67‑1 (MQ=255)
agaaAATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCGCCGCATGAAGTTATGCTGACTATTGAt > 1:1237225/1‑68 (MQ=255)
agaaAATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCGCCGCATGAAGTTATGCTGACTATTGAt > 1:576371/1‑68 (MQ=255)
agaaAATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCGCCGCATGAAGTTATGCTGACTATTGAt > 1:3073715/1‑68 (MQ=255)
agaaAATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCGCCGCATGAAGTTATGCTGACTATTGAt > 1:30552/1‑68 (MQ=255)
agaaAATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCGCCGCATGAAGTTATGCTGACTATTGAt > 1:1917018/1‑68 (MQ=255)
agaaAATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCGCCGCATGAAGTTATGCTGACTATTGAt > 1:2367645/1‑68 (MQ=255)
agaaAATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCGCCGCATGAAGTTATGCTGACTATTGAt > 1:944945/1‑68 (MQ=255)
aaaaTCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCTCCGCATGAAGTTATGCTGACTATTGATGcc > 1:1974087/1‑69 (MQ=255)
aaaaTCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCTCCGCATGAAGTTATGCTGACTATTGATGcc > 1:2622780/1‑69 (MQ=255)
aaaaTCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCTCCGCATGAAGTTATGCTGACTATTGATGcc > 1:1687968/1‑69 (MQ=255)
aaaaTCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCTCCGCATGAAGTTATGCTGACTATTGATGcc > 1:2789964/1‑69 (MQ=255)
aaaaTCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCTCCGCATGAAGTTATGCTGACTATTGATGcc > 1:2436310/1‑69 (MQ=255)
aaaaTCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCTCCGCATGAAGTTATGCTGACTATTGATGcc > 1:1139652/1‑69 (MQ=255)
aaaaTCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCTCCGCATGAAGTTATGCTGACTATTGATGcc > 1:2982666/1‑69 (MQ=255)
aaaaTCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCTCCGCATGAAGTTATGCTGACTATTGATGcc > 1:2261990/1‑69 (MQ=255)
aaaaTCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCTCCGCATGAAGTTATGCTGACTATTGATGc > 1:1191582/1‑68 (MQ=255)
aaaaTCGTCCGCGTGATGAAGAAACTCGACGTT‑AAGCTCCGCATGAAGTTATGCTGACTATTGATGcc > 1:1541508/1‑68 (MQ=255)
aaaTCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCGCCGCATGAAGTTa < 1:2710905/51‑1 (MQ=255)
aaTCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCGCCGCATGAAGTTATGCTGACTATTGATGCCAg < 1:656411/69‑1 (MQ=255)
gTCCGCGTGATGAAGAAACTCGACGTTGAAGCTCCGCTTGAAGTTATGCTGACTAt < 1:569361/56‑1 (MQ=255)
cgcgTGATGAAGAAACTCGACGTTGAAGCGCCGCATGAAGTTATGCTGACTATTGATGCCAGCACCggg < 1:914230/69‑1 (MQ=255)
cgcgTGATGAAGAAACTCGACGTTGAAGCGCCGCATGAAGTTATGCTGACTATTGATGCCAGCACCggg < 1:233760/69‑1 (MQ=255)
cgcgTGATGAAGAAACTCGACGTTGAAGCGCCGCATGAAGTTATGCTGACTATTGATGCCAGCACCggg < 1:2065859/69‑1 (MQ=255)
gtagaaACTCGTCGTTGAAGCTCCGCATGAAGTTATGCTGACTAt < 1:15200/43‑1 (MQ=37)
gaagaaACTCGACGTTGAAGCTCCGCATGAAGTTATGCTGACTAt < 1:891896/45‑1 (MQ=255)
gaaACTCGACGTTGAAGCGCCGCATGAAGTTATGCTGACTATTGATGCCAGCACCGGGCAGAACGCGGt < 1:904466/69‑1 (MQ=255)
cTCGACGTTGAAGCTCCGCATGAAGTTATGCTGACTAt < 1:1828252/38‑1 (MQ=255)
cTCGACGTTGAAGCGCCGCATGAAGTTATGCTGACTATTGATGCCAGCACCGGGCAGAACGCTGTAAGc < 1:2377269/69‑1 (MQ=255)
cTCGACGTTGAAGCGCCGCATGAAGTTATGCTGACTATTGATGCCAGCACCGGGCAGAACGCGGTAAGc < 1:1654670/69‑1 (MQ=255)
cTCGACGTTGAAGCGCCGCATGAAGTTATGCTGACTATTGATGCCAGCACCGGGCAGAACGCGGTAAGc < 1:979402/69‑1 (MQ=255)
cGACGTTGAAGCGCCGCATGAAGTTATGCTGACTATTGATGCCAGCACCGGGCAGAACGCGGt < 1:3434235/63‑1 (MQ=255)
ttGATGCGCCGCATGAAGTTATGCTGACTATTGATGCCAGCACCggg < 1:2013711/47‑1 (MQ=255)
ttGAAGCGCCGCATGAAGTTATGCTGACTATTGATGCCAGCACCGGGCAGAACGCGGTAAGCCAGGCCa < 1:1987227/69‑1 (MQ=255)
ttGAAGCGCCGCATGAAGTTATGCTGACTATTGATGCCAGCACCGGGCAGAACGCGGTAAGCCAGGCCa < 1:1033873/69‑1 (MQ=255)
gAAGCGCCGCATGAAGTTATGCTGACTATTGATGCCAGCACCGGGCAGAACGCGGTAAGCCAGGCCaaa > 1:3092914/1‑69 (MQ=255)
|
CAAATCGCACCTGATGGAAGAGTTGAAGAAAATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCGCCGCATGAAGTTATGCTGACTATTGATGCCAGCACCGGGCAGAACGCGGTAAGCCAGGCCAAA > minE/2624112‑2624241
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
