Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,652,018 |
T→C |
56.1% |
I84I (ATT→ATC) |
glgC → |
glucose‑1‑phosphate adenylyltransferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,652,018 | 0 | T | C | 56.1%
| 16.7
/ 49.2
| 66 | I84I (ATT→ATC) | glgC | glucose‑1‑phosphate adenylyltransferase |
| Reads supporting (aligned to +/- strand): ref base T (16/13); new base C (31/6); total (47/19) |
| Fisher's exact test for biased strand distribution p-value = 1.45e-02 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.64e-01 |
AACTCCGGGATCCGTCGTATGGGCGTGATCACCCAGTACCAGTCCCACACTCTGGTGCAGCACATTCAGCGCGGCTGGTCATTCTTCAATGAAGAAATGAACGAGTTTGTCGATCTGCTGCCAGCACAGCAGAG > minE/2651953‑2652086
|
aaCTCCGGGATCCGTCGTATGGGCGTGATCACCCAGTACCAGTCCCACACTCTGGTGCAGCACATTCAg > 1:696793/1‑69 (MQ=255)
aaCTCCGGGATCCGTCGTATGGGCGTGATCACCCAGTACCAGTCCCACACTCTGGTGCAGCACATTCAg > 1:1826941/1‑69 (MQ=255)
aaCTCCGGGATCCGTCGTATGGGCGTGATCACCCAGTACCAGTCCCACACTCTGGTGCAGCACATTCAg > 1:2309937/1‑69 (MQ=255)
cgtcgtATGGGCGTGATCACCCAGTACCAGTCCCACACTCTGGTGCAGCACATTCAGCGCGGCTGGtaa < 1:2428650/69‑3 (MQ=255)
cgtcgtATGGGCGTGATCACCCAGTACCAGTCCCACACTCTGGTGCAGCACATTCAGCGCGGCTGGTCa < 1:1756374/69‑1 (MQ=255)
aTCACCGAGTACCAGTCCCACACTCTGGTGCAGCACATTCAGCGCGGCTGGTCATTCTTCAATgaagaa > 1:439251/1‑69 (MQ=255)
aTCACCCAGTACCAGTCCCACACTCTGGTGCAGCACATTCAGCGCGGCTGGTCATTCTTCAATgaagaa > 1:1116377/1‑69 (MQ=255)
aTCACCCAGTACCAGTCCCACACTCTGGTGCAGCACATTCAGCGCGGCTGGTCATTCTTCAATgaagaa > 1:3235705/1‑69 (MQ=255)
aTCACCCAGTACCAGTCCCACACTCTGGTGCAGCACATTCAGCGCGGCTGGTCATTCTTCAATgaagaa > 1:3185006/1‑69 (MQ=255)
aTCACCCAGTACCAGTCCCACACTCTGGTGCAGCACATTCAGCGCGGCTGGTCATTCTTCAATgaagaa > 1:2469215/1‑69 (MQ=255)
aTCACCCAGTACCAGTCCCACACTCTGGTGCAGCACATTCAGCGCGGCTGGTCATTCTTCAATgaagaa > 1:1693368/1‑69 (MQ=255)
aTCACCCAGTACCAGTCCCACACTCTGGTGCAGCACATTCAGCGCGGCTGGTCATTCTTCAATgaaga > 1:970537/1‑68 (MQ=255)
cACCCAGTACCAGTCCCACACTCTGGTGCAGCACATCCAGCGCGGCTGGTCATTCTTCAATGAAGaaa > 1:2918498/1‑68 (MQ=255)
cACCCAGTACCAGTCCCACACTCTGGTGCAGCACATCCAGCGCGGCTGGTCATTCTTCAATGAAGaaa > 1:2152700/1‑68 (MQ=255)
cACCCAGTACCAGTCCCACACTCTGGTGCAGCACATCCAGCGCGGCTGGTCATTCTTCAATGAAGaaa > 1:2181579/1‑68 (MQ=255)
cACCCAGTACCAGTCCCACACTCTGGTGCAGCACATCCAGCGCGGCTGGTCATTCTTCAATGAAGaaa > 1:2170270/1‑68 (MQ=255)
cACCCAGTACCAGTCCCACACTCTGGTGCAGCACATCCAGCGCGGCTGGTCATTCTTCAATGAAGaaa > 1:110235/1‑68 (MQ=255)
cACCCAGTACCAGTCCCACACTCTGGTGCAGCACATCCAGCGCGGCTGGTCATTCTTCAATGAAGaaa > 1:2927282/1‑68 (MQ=255)
cACCCAGTACCAGTCCCACACTCTGGTGCAGCACATCCAGCGCGGCTGGTCATTCTTCAATGAAGaaa > 1:2932503/1‑68 (MQ=255)
cACCCAGTACCAGTCCCACACTCTGGTGCAGCACATCCAGCGCGGCTGGTCATTCTTCAATGAAGaaa > 1:2936141/1‑68 (MQ=255)
cACCCAGTACCAGTCCCACACTCTGGTGCAGCACATCCAGCGCGGCTGGTCATTCTTCAATGAAGaaa > 1:3054695/1‑68 (MQ=255)
cACCCAGTACCAGTCCCACACTCTGGTGCAGCACATCCAGCGCGGCTGGTCATTCTTCAATGAAGaaa > 1:3135469/1‑68 (MQ=255)
cACCCAGTACCAGTCCCACACTCTGGTGCAGCACATCCAGCGCGGCTGGTCATTCTTCAATGAAGaaa > 1:3220804/1‑68 (MQ=255)
cACCCAGTACCAGTCCCACACTCTGGTGCAGCACATCCAGCGCGGCTGGTCATTCTTCAATGAAGaaa > 1:383208/1‑68 (MQ=255)
cACCCAGTACCAGTCCCACACTCTGGTGCAGCACATCCAGCGCGGCTGGTCATTCTTCAATGAAGaaa > 1:5143/1‑68 (MQ=255)
cACCCAGTACCAGTCCCACACTCTGGTGCAGCACATCCAGCGCGGCTGGTCATTCTTCAATGAAGaaa > 1:1649043/1‑68 (MQ=255)
cACCCAGTACCAGTCCCACACTCTGGTGCAGCACATCCAGCGCGGCTGGTCATTCTTCAATGAAGaaa > 1:1590369/1‑68 (MQ=255)
cACCCAGTACCAGTCCCACACTCTGGTGCAGCACATCCAGCGCGGCTGGTCATTCTTCAATGAAGaaa > 1:1782094/1‑68 (MQ=255)
cACCCAGTACCAGTCCCACACTCTGGTGCAGCACATCCAGCGCGGCTGGTCATTCTTCAATGAAGaaa > 1:1956797/1‑68 (MQ=255)
cACCCAGTACCAGTCCCACACTCTGGTGCAGCACATCCAGCGCGGCTGGTCATTCTTCAATGAAGaaa > 1:1137480/1‑68 (MQ=255)
cACCCAGTACCAGTCCCACACTCTGGTGCAGCACATCCAGCGCGGCTGGTCATTCTTCAATGAAGaaa > 1:1423511/1‑68 (MQ=255)
gTACCAGTCCCACACTCTGGTGCAGCACATTCAGCGCGGCTGGTCATTCTTCAATGAAGAAATGAACGa < 1:2161598/69‑1 (MQ=255)
gTACCAGTCCCACACTCTGGTGCAGCACATTCAGCGCGGCTGGTCATTCTTCAATGAAGAAATGAACGa < 1:305244/69‑1 (MQ=255)
gTACCAGTCCCACACTCTGGTGCAGCACATTCAGCGCGGCTGGTCATTCTTCAATGAAGAAATGAACGa < 1:2947770/69‑1 (MQ=255)
gTACCAGTCCCACACTCTGGTGCAGCACATTCAGCGCGGCTGGTCATTCTTCAATGAAGAAATGAACGa < 1:347598/69‑1 (MQ=255)
gTACCAGTCCCACACTCTGGTGCAGCACATTCAGCGCGGCTGGTCATTCTTCAATGAAGAAATGAACGa < 1:2079395/69‑1 (MQ=255)
gTACCAGTCCCACACTCTGGTGCAGCACATTAAGCGCGGCTGGTCATTCTTCAATGAAGAAATGAACGa < 1:1247316/69‑1 (MQ=255)
taacAGTCCCACACTCTGGTGCAGCACATTCAGCGCGGCTGGTCATTCTTCAATGAAGAAATGAACGAg < 1:2412654/66‑1 (MQ=255)
cccACACTCTGGTGCAGCACATTCAGCGCGGCTGGTCATTCTTCAATGAAGAAATGAACGAGTTTGTCg < 1:478741/69‑1 (MQ=255)
cccACACTCTGGTGCAGCACATTCAGCGCGGCTGGTCATTCTTCAATGAAGAAATGAACGAGTTTGTCg > 1:811799/1‑69 (MQ=255)
acacTCTGGTGCAGCACATCCAGCGCGGCTGGTCa > 1:1338156/1‑35 (MQ=255)
acacTCTGGTGCAGCACATCCAGCGCGGCTGGTCa > 1:527759/1‑35 (MQ=255)
acacTCTGGTGCAGCACATCCAGCGCGGCTGGTCa > 1:3375761/1‑35 (MQ=255)
acacTCTGGTGCAGCACATCCAGCGCGGCTGGTCa > 1:720599/1‑35 (MQ=255)
acacTCTGGTGCAGCACATCCAGCGCGGCTGGTCa > 1:1531278/1‑35 (MQ=255)
acacTCTGGTGCAGCACATCCAGCGCGGCTGGTCa > 1:776671/1‑35 (MQ=255)
acacTCTGGTGCAGCACATCCAGCGCGGCTGGTCa > 1:105928/1‑35 (MQ=255)
acacTCTGGTGCAGCACATCCAGCGCGGCTGGTCa > 1:1890125/1‑35 (MQ=255)
acacTCTGGTGCAGCACATCCAGCGCGGCTGGTCa > 1:1776291/1‑35 (MQ=255)
acacTCTGGTGCAGCACATCCAGCGCGGCTGGTCa > 1:1843615/1‑35 (MQ=255)
acacTCTGGTGCAGCACATCCAGCGCGGCTGGTCa > 1:227564/1‑35 (MQ=255)
acacTCTGGTGCAGCACATCCAGCGCGGCTGGTCa > 1:2693298/1‑35 (MQ=255)
ctctGGTGCAGTACATCCAGCGCGGCTGGTCATTCTTCAATGAAGAAATGAACGAGTTTGTCGATctgc < 1:1387822/69‑1 (MQ=255)
ctctGGTGCAGCACATTCAGCGCGGCTGGTCATTCTTCAATGAAGAAATGAACGAGTTTGTCGATctgc > 1:321469/1‑69 (MQ=255)
ctctGGTGCAGCACATTCAGCGCGGCTGGTCATTCTTCAATGAAGAAATGAACGAGTTTGTCGATctgc < 1:407206/69‑1 (MQ=255)
ctctGGTGCAGCACATCCAGCGCGGCTGGTCATTCTTCAATGAAGAAATGAACGAGTTTGTCGATctgc < 1:2950935/69‑1 (MQ=255)
ctctGGTGCAGCACATCCAGCGCGGCTGGTCATTCTTCAATGAAGAAATGAACGAGTTTGTCGATctgc < 1:1711543/69‑1 (MQ=255)
ctctGGTGCAGCACATCCAGCGCGGCTGGTCATTCTTCAATGAAGAAATGAACGAGTTTGTCGATctgc < 1:814019/69‑1 (MQ=255)
ctctGGTGCAGCACATCCAGCGCGGCTGGTCATTCTTCAATGAAGAAATGAACGAGTTTGTCGATctgc < 1:2884190/69‑1 (MQ=255)
tctGGTGCAGCACATTCAGCGCGGCTGGTCATTCTTCAATGAAGAAATGAACGAGTTTGTCGATctgct < 1:3407668/69‑1 (MQ=255)
tctGGTGCAGCACATCCAGCGCGGCTGGTCATTCTTCAATGAAGAAATGAACGAGTTTGTCGATctgc < 1:606070/68‑1 (MQ=255)
cagcaCATTCAGCGCGGCTGGTCATTCTTCAATGAAGAAATGAACGa < 1:2460901/47‑1 (MQ=255)
cagcaCATTCAGCGCGGCTGGTCATTCTTCAATGAAGAAATGAACGAGTTTGTCGATCTGCTGCcag > 1:345245/1‑67 (MQ=255)
cagcaCATTCAGCGCGGCTGGTCATTCTTCAATGAAGAAATGAACGAGTTTGTCGATCTGCTGCcag > 1:3149976/1‑67 (MQ=255)
acaTTCAGCGCGGCTGGTCATTCTTCAATGAAGAAATGAACGAGTTTGTCGATCTGCTGCcagcac > 1:2693155/1‑66 (MQ=255)
caTTCAGCGCGGCTGGTCATTCTTCAATGAAGAAATGAACGAGTTTGTCGATCTGCTGCcagcacag > 1:2031106/1‑67 (MQ=255)
tCAGCGCGGCTGGTCATTCTTCAATGAAGAAATGAACGAGTTTGTCGATCTGCTGCCAGCACAGCagag < 1:2113214/69‑1 (MQ=255)
tCAGCGCGGCTGGTCATTCTTCAATGAAGAAATGAACGAGTTTGTCGATCTGCTGCCAGCACAGCagag < 1:811135/69‑1 (MQ=255)
tCAGCGCGGCTGGTCATTCTTCAATGAAGAAATGAACGAGTTTGTCGATCTGCTGCCAGCACAGCagag < 1:2281354/69‑1 (MQ=255)
tCAGCGCGGCTGGTCATTCTTCAATGAAGAAATGAACGAGTTTGTCGATCTGCTGCCAGCACAGCagag < 1:2355651/69‑1 (MQ=255)
tCAGCGCGGCTGGTCATTCTTCAATGAAGAAATGAACGAGTTTGTCGATCTGCTGCCAGCACAGCagag < 1:670894/69‑1 (MQ=255)
tCAGCGCGGCTGGTCATTCTTCAATGAAGAAATGAACGAGTTTGTCGATCTGCTGCCAGCACAGCagag < 1:1288320/69‑1 (MQ=255)
tCAGCGCGGCTGGTCATTCTTCAATGAAGAAATGAACGAGTTTGTCGATCTGCTGCCAGCACAGCagag < 1:381485/69‑1 (MQ=255)
tCAGCGCGGCTGGTCATTCTTCAATGAAGAAATGAACGAGTTTGTCGATCTGCTGCCAGCACAGCagag < 1:2847559/69‑1 (MQ=255)
tCAGCGCGGCTGGTCATTCTTCAATGAAGAAATGAACGAGTTTGTCGATCTGCTGCCAGCACAGCagag < 1:3244031/69‑1 (MQ=255)
tCAGCGCGGCTGGTCATTCTTCAATGAAGAAATGAACGAGTTTGTCGATCTGCTGCCAGCACAGCagag < 1:3150704/69‑1 (MQ=255)
tCAGCGCGGCTGGTCATTCTTCAATGAAGAAATGAACGAGTTTGTCGATCTGCTGCCAGCACAGCagag < 1:1691259/69‑1 (MQ=255)
|
AACTCCGGGATCCGTCGTATGGGCGTGATCACCCAGTACCAGTCCCACACTCTGGTGCAGCACATTCAGCGCGGCTGGTCATTCTTCAATGAAGAAATGAACGAGTTTGTCGATCTGCTGCCAGCACAGCAGAG > minE/2651953‑2652086
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
