Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F1 I0 R1 4577 57.4 3436498 77.7% 2670158 63.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,664,129 A→G 29.4% E371E (GAA→GAG)  rtcB → conserved hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,664,1290AG29.4% 119.1 / 57.2 122E371E (GAA→GAG) rtcBconserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (23/63);  new base G (9/27);  total (32/90)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.99e-01

Only 100 of 122 total aligned reads displayed.

AAACTGTTCAGCGTGGAAGATCAAATTCGTGCCACCGCGCATGTGGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATCCCGATGGCGTATAAAGATATTGATGCGGTGATGGCGGCACAAAGCGA  >  minE/2664064‑2664191
                                                                 |                                                              
aaaCTGTTCAGTGTGGAAGATCAAATCCGTGCCTCCGCGCATGTGGAGTGCCGTAAAGATGCCGAGGTg                                                             <  1:3324780/69‑1 (MQ=255)
aaaCTGTTCAGTGTGGAAGATCAAATCCGTGCCACCGCGCATGTGGAGTGCCGTAAAGATGCCGAGGTg                                                             <  1:1843373/69‑1 (MQ=255)
aaaCTGTTCAGTGTGGAAGATCAAATCCGTGCCACCGCGCATGTGGAGTGCCGTAAAGATGCCGAGGTg                                                             <  1:2526938/69‑1 (MQ=255)
aaaCTGTTCAGTGTGGAAGATCAAATCCGTGCCACCGCGCATGTGGAGTGCCGTAAAGATGCCGAGGTg                                                             <  1:2596575/69‑1 (MQ=255)
aaaCTGTTCAGTGTGGAAGATCAAATCCGTGCCACCGCGCATGTGGAGTGCCGTAAAGATGCCGAGGTg                                                             <  1:2631344/69‑1 (MQ=255)
aaaCTGTTCAGTGTGGAAGATCAAATCCGTGCCACCGCGCATGTGGAGTGCCGTAAAGATGCCGAGGTg                                                             <  1:248441/69‑1 (MQ=255)
aaaCTGTTCAGTGTGGAAGATCAAATCCGTGCCACCGCGCATGTGGAGTGCCGTAAAGATGCCGAGGTg                                                             <  1:2752991/69‑1 (MQ=255)
aaaCTGTTCAGTGTGGAAGATCAAATCCGTGCCACCGCGCATGTGGAGTGCCGTAAAGATGCCGAGGTg                                                             <  1:2092924/69‑1 (MQ=255)
aaaCTGTTCAGTGTGGAAGATCAAATCCGTGCCACCGCGCATGTGGAGTGCCGTAAAGATGCCGAGGTg                                                             <  1:1862759/69‑1 (MQ=255)
aaaCTGTTCAGTGTGGAAGATCAAATCCGTGCCACCGCGCATGTGGAGTGCCGTAAAGATGCCGAGGTg                                                             <  1:57112/69‑1 (MQ=255)
aaaCTGTTCAGTGTGGAAGATCAAATCCGTGCCACCGCGCATGTGGAGTGCCGTAAAGATGCCGAGGTg                                                             <  1:1518255/69‑1 (MQ=255)
aaaCTGTTCAGTGTGGAAGATCAAATCCGTGCCACCGCGCATGTGGAGTGCCGTAAAGATGCCGAGGTg                                                             <  1:62608/69‑1 (MQ=255)
aaaCTGTTCAGTGTGGAAGATCAAATCCGTGCCACCGCGCATGTGGAGTGCCGTAAAGATGCCGAGGTg                                                             <  1:915278/69‑1 (MQ=255)
aaaCTGTTCAGTGTGGAAGATCAAATCCGTGCCACCGCGCATGTGGAGTGCCGTAAAGATGCCGAGGTg                                                             <  1:1085342/69‑1 (MQ=255)
aaaCTGTTCAGTGTGGAAGATCAAATCCGTGCCACCGCGCATGTGGAGTGCCGTAAAGATGCCGAGGTg                                                             <  1:744524/69‑1 (MQ=255)
aaaCTGTTCAGTGTGGAAGATCAAATCCGTGCCACCGCGCATGTGGAGTGCCGTAAAGATGCCGAGGTg                                                             <  1:83337/69‑1 (MQ=255)
aaaCTGTTCAGCGTGGAAGATCAAATTCGTGCCACCGCGCATGTGGAATGCCGTAAAGATGCCGAAGTg                                                             <  1:3223149/69‑1 (MQ=255)
aaaCTGTTCAGCGTGGAAGATCAAATTCGTGCCACCGCGCATGTGGAATGCCGTAAAGATGCCGAAGTg                                                             <  1:2137873/69‑1 (MQ=255)
aaaCTGTTCAGCGTGGAAGATCAAATTCGTGCCACCGCGCATGTGGAATGCCGTAAAGATGCCGAAGTg                                                             <  1:835602/69‑1 (MQ=255)
 aaCTGTTCAGTGTGGAAGATCAAATCCGTGCCACCGCGCATGTGGAGTGCCGTAAAGATGCCGAGGTg                                                             <  1:3088167/68‑1 (MQ=255)
 aaCTGTTCAGTGTGGAAGATCAAATCCGTGCCACCGCGCATGTGGAGTGCCGTAAAGATGCCGAGGTg                                                             <  1:2154657/68‑1 (MQ=255)
        cAGCGTGGAAGATCAAATTCGTGCCACCGCGCATGTGGAATGCCGTAAAGATGCCGAAGTGATcgacga                                                     >  1:818359/1‑69 (MQ=255)
        cAGCGTGGAAGATCAAATTCGTGCCACCGCGCATGTGGAATGCCGTAAAGATGCCGAAGTGATcgacga                                                     >  1:1730956/1‑69 (MQ=255)
        cAGCGTGGAAGATCAAATTCGTGCCACCGCGCATGTGGAATGCCGTAAAGATGCCGAAGTGATcgacga                                                     >  1:287603/1‑69 (MQ=255)
        cAGCGTGGAAGATCAAATTCGTGCCACCGCGCATGTGGAATGCCGTAAAGATGCCGAAGTGATcgacga                                                     >  1:1105733/1‑69 (MQ=255)
        cAGCGTGGAAGATCAAATTCGTGCCACCGCGCATGTGGAATGCCGTAAAGATGCCGAAGTGATcgacga                                                     >  1:3183916/1‑69 (MQ=255)
                 aGATCAAATTCGTGCCACCGCGCATGTGGAATGCCGTAAAGATGCCGAAGTGATcgacga                                                     <  1:1340188/60‑1 (MQ=255)
                 aGATCAAATTCGTGCCACCGCGCATGTGGAATGCCGTAAAGATGCCGAAGTGATcgacga                                                     <  1:2416677/60‑1 (MQ=255)
                 aGATCAAATTCGTGCCACCGCGCATGTGGAATGCCGTAAAGATGCCGAAGTGATcgacga                                                     <  1:3176446/60‑1 (MQ=255)
                 aGATCAAATTCGTGCCACCGCGCATGTGGAATGCCGTAAAGATGCCGAAGTGATcgacga                                                     >  1:1502619/1‑60 (MQ=255)
                 aGATCAAATTCGTGCCACCGCGCATGTGGAATGCCGTAAAGATGCCGAAGTGATcgacga                                                     <  1:118923/60‑1 (MQ=255)
                 aGATCAAATTCGTGCCACCGCGCATGTGGAATGCCGTAAAGATGCCGAAGTGATcgacga                                                     <  1:3289574/60‑1 (MQ=255)
                 aGATCAAATTCGTGCCACCGCGCATGTGGAATGCCGTAAAGATGCCGAAGTGATcgacga                                                     <  1:2683461/60‑1 (MQ=255)
                 aGATCAAATTCGTGCCACCGCGCATGTGGAATGCCGTAAAGATGCCGAAGTGATcgacga                                                     >  1:897552/1‑60 (MQ=255)
                 aGATCAAATTCGTGCCACCGCGCATGTGGAATGCCGTAAAGATGCCGAAGTGATcgacga                                                     <  1:1424470/60‑1 (MQ=255)
                 aGATCAAATTCGTGCCACCGCGCATGTGGAATGCCGTAAAGATGCCGAAGTGATcgacga                                                     <  1:1817821/60‑1 (MQ=255)
                 aGATCAAATTCGTGCCACCGCGCATGTGGAATGCCGTAAAGATGCCGAAGTGATcgacga                                                     <  1:1780043/60‑1 (MQ=255)
                 aGATCAAATTCGTGCCACCGCGCATGTGGAATGCCGTAAAGATGCCGAAGTGATcgacga                                                     <  1:1581405/60‑1 (MQ=255)
                     cAAATTCGTGCCACCGCGCATGTGGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATCCCGATGGCg                                        >  1:1469682/1‑69 (MQ=255)
                           cGTGCCACCGCGCATGTGGAGTGCCGTAAAGATGCCGAGGTGATCGACGAAATCCCGATGGCGTATaaa                                  <  1:3206180/69‑1 (MQ=255)
                           cGTGCCACCGCGCATGTGGAGTGCCGTAAAGATGCCGAGGTGATCGACGAAATCCCGATGGCGTATaaa                                  <  1:1907338/69‑1 (MQ=255)
                           cGTGCCACCGCGCATGTGGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATCCCGATGGCGta                                      >  1:614224/1‑65 (MQ=255)
                           cGTGCCACCGCGCATGTGGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATCCCGATGGCGta                                      >  1:2472249/1‑65 (MQ=255)
                           cGTGCCACCGCGCATGTGGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATCCCGATGGCGta                                      >  1:1993731/1‑65 (MQ=255)
                                cACCGCGCATGTGGAGTGCCGTAAAGATGCCGAGGTGATCGACGAAATCCCGATGGCGTATAAAGata                              >  1:2006088/1‑68 (MQ=255)
                                cACCGCGCATGTGGAGTGCCGTAAAGATGCCGAGGTGATCGACGAAATCCCGATGGCGTATAAAGata                              >  1:2701969/1‑68 (MQ=255)
                                cACCGCGCATGTGGAGTGCCGTAAAGATGCCGAGGTGATCGACGAAATCCCGATGGCGTATAAAGata                              >  1:1516802/1‑68 (MQ=255)
                                cACCGCGCATGTGGAGTGCCGTAAAGATGCCGAGGTGATCGACGAAATCCCGATGGCGTATAAAGata                              >  1:434505/1‑68 (MQ=255)
                                cACCGCGCATGTGGAGTGCCGTAAAGATGCCGAGGTGATCGACGAAATCCCGATGGCGTATAAAGata                              >  1:1073364/1‑68 (MQ=255)
                                cACCGCGCATGTGGAGTGCCGTAAAGATGCCGAGGTGATCGACGAAATCCCGATGGCGTATAAAGata                              >  1:776836/1‑68 (MQ=255)
                                cACCGCGCATGTGGAGTGCCGTAAAGATGCCGAGGTGATCGACGAAATCCCGATGGCGTATAAAGat                               >  1:1046120/1‑67 (MQ=255)
                                         tgtgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATCCCGATGGCGTATAAAGATATTGATGCGGt                    <  1:1634910/69‑1 (MQ=255)
                                           tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATccc                                               <  1:1201055/40‑1 (MQ=255)
                                           tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATccc                                               <  1:1225016/40‑1 (MQ=255)
                                           tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATccc                                               <  1:3131348/40‑1 (MQ=255)
                                           tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATccc                                               <  1:1307459/40‑1 (MQ=255)
                                           tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATccc                                               <  1:1212417/40‑1 (MQ=255)
                                           tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATccc                                               <  1:51863/40‑1 (MQ=255)
                                           tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATccc                                               <  1:74198/40‑1 (MQ=255)
                                           tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATccc                                               <  1:1008592/40‑1 (MQ=255)
                                           tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATccc                                               <  1:614934/40‑1 (MQ=255)
                                           tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATccc                                               <  1:2501553/40‑1 (MQ=255)
                                           tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATccc                                               <  1:706687/40‑1 (MQ=255)
                                           tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATCCCGATGGCGta                                      <  1:583005/49‑1 (MQ=255)
                                           tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATCCCGATGGCGta                                      <  1:3158040/49‑1 (MQ=255)
                                           tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATCCCGATGGCGta                                      <  1:186636/49‑1 (MQ=255)
                                           tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATCCCGATGGCGta                                      <  1:3377167/49‑1 (MQ=255)
                                           tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATCCCGATGGCGta                                      <  1:1472840/49‑1 (MQ=255)
                                           tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATCCCGATGGCGta                                      <  1:1290134/49‑1 (MQ=255)
                                           tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATCCCGATGGCGta                                      <  1:1921600/49‑1 (MQ=255)
                                           tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATCCCGATGGCGta                                      <  1:1924665/49‑1 (MQ=255)
                                           tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATCCCGATGGCGta                                      <  1:196277/49‑1 (MQ=255)
                                           tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATCCCGATGGCGta                                      <  1:2230773/49‑1 (MQ=255)
                                           tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATCCCGATGGCGta                                      <  1:2408913/49‑1 (MQ=255)
                                           tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATCCCGATGGCGta                                      <  1:2458406/49‑1 (MQ=255)
                                           tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATCCCGATGGCGta                                      <  1:2485190/49‑1 (MQ=255)
                                           tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATCCCGATGGCGTATAAAGATATTGATGCGGt                    <  1:3231815/67‑1 (MQ=255)
                                           tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATCCCGATGGCGTATAAAGATATTGATGCGGt                    <  1:3134205/67‑1 (MQ=255)
                                           tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATCCCGATGGCGTATAAAGATATTGATGCGGt                    <  1:3130450/67‑1 (MQ=255)
                                           tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATCCCGATGGCGTATAAAGATATTGATGCGGt                    >  1:2306040/1‑67 (MQ=255)
                                           tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATCCCGATGGCGTATAAAGATATTGATGCGGt                    <  1:295797/67‑1 (MQ=255)
                                           tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATCCCGATGGCGTATAAAGATATTGATGCGGt                    <  1:2717046/67‑1 (MQ=255)
                                           tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATCCCGATGGCGTATAAAGATATTGATGCGGt                    >  1:990225/1‑67 (MQ=255)
                                           tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATCCCGATGGCGTATAAAGATATTGATGCGGt                    <  1:2562760/67‑1 (MQ=255)
                                           tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATCCCGATGGCGTATAAAGATATTGATGCGGTGa                  >  1:2508357/1‑69 (MQ=255)
                                           tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATCCCGATGGCGTATAAAGATATTGATGCGGTGa                  >  1:874947/1‑69 (MQ=255)
                                           tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATCCCGATGGCGTATAAAGATATTGATGCGGTGa                  >  1:630786/1‑69 (MQ=255)
                                           tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATCCCGATGGCGTATAAAGATATTGATGCGGTGa                  >  1:1920533/1‑69 (MQ=255)
                                           tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATCCCGATGGCGTATAAAGATATTGATGCGGTGa                  >  1:2766817/1‑69 (MQ=255)
                                           tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATACCGATGGCGTATAAAGATATTGATGCGGt                    >  1:962891/1‑67 (MQ=255)
                                           tgGAATGCCGTAAAGATGCCAAAGTGATCGACGAAATCCCGATGGCGta                                      <  1:2702174/49‑1 (MQ=255)
                                           tgGAATGCCGTAAAGATGACGAAGTGATCGACGAAATCCCGATGGCGTATAAAGATATTGATGCGGt                    >  1:2437604/1‑67 (MQ=255)
                                            ggAATGCCGTAAAGATGCCGAAGTGATCGACGAAATccc                                               <  1:1665573/39‑1 (MQ=255)
                                                       aaGATGCCGAAGTGATCGACGAAATCCCGATGGCGTATAAAGa                                <  1:733096/43‑1 (MQ=255)
                                                       aaGATGCCGAAGTGATCGACGAAATCCCGATGGCGTATAAAGa                                <  1:1057967/43‑1 (MQ=255)
                                                         gATGCCGAGGTGATCGACGAAATCCCGATGGCGTATaaa                                  <  1:2890656/39‑1 (MQ=255)
                                                           tGCCGAAGTGATCGACGAAATCCCGATGGCGTATAAAGATATTGATGCGGTGATGGCGGCACAAAGCGa  <  1:719153/69‑1 (MQ=255)
                                                           tGCCGAAGTGATCGACGAAATCCCGATGGCGTATAAAGATATTGATGCGGTGATGGCGGCACAAAGCGa  <  1:3306082/69‑1 (MQ=255)
                                                           tGCCGAAGTGATCGACGAAATCCCGATGGCGTATAAAGATATTGATGCGGTGATGGCGGCACAAAGCGa  <  1:1996339/69‑1 (MQ=255)
                                                           tGCCGAAGTGATCGACGAAATCCCGATGGCGTATAAAGATATTGATGCGGTGATGGCGGCACAAAGCGa  <  1:2936719/69‑1 (MQ=255)
                                                                 |                                                              
AAACTGTTCAGCGTGGAAGATCAAATTCGTGCCACCGCGCATGTGGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATCCCGATGGCGTATAAAGATATTGATGCGGTGATGGCGGCACAAAGCGA  >  minE/2664064‑2664191

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A