Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,668,922 |
G→T |
35.3% |
T100T (ACG→ACT) |
malP → |
maltodextrin phosphorylase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,668,922 | 0 | G | T | 35.3%
| 80.6
/ 58.9
| 82 | T100T (ACG→ACT) | malP | maltodextrin phosphorylase |
| Reads supporting (aligned to +/- strand): ref base G (24/29); new base T (17/12); total (41/41) |
| Fisher's exact test for biased strand distribution p-value = 3.56e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.74e-01 |
GAATCTCGGCTGGTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAATCTGACGGACCTGCTGGAAGAAGAGATCGACCCGGCGCTGGGTAACGGTGGTCTGGGACGTCTGGCGGC > minE/2668862‑2668984
|
gAATCTCGGCTGGTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAATCTGACGGACctgc > 1:1116569/1‑68 (MQ=255)
cTGGTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAATCTGACGGACCTGCTGgaag < 1:835965/65‑1 (MQ=255)
cTGGTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAATCTGACGGACCTGCTGgaag < 1:3016810/65‑1 (MQ=255)
cTGGTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAATCTGACGGACCTGCTGgaag < 1:1721707/65‑1 (MQ=255)
cTGGTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAATCTGACGGACCTGCTGgaag < 1:1198855/65‑1 (MQ=255)
cTGGTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAATCTGACGGACCTGCTGgaag < 1:2495481/65‑1 (MQ=255)
cTGGTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAATCTGACGGACCTGCTGgaag < 1:2436813/65‑1 (MQ=255)
cTGGTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAATCTGACGGACCTGCTGgaag < 1:744127/65‑1 (MQ=255)
cTGGTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAATCTGACGGACCTGCTGgaag < 1:2089533/65‑1 (MQ=255)
cTGGTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAATCTGACGGACCTGCTGgaag < 1:2328443/65‑1 (MQ=255)
cTGGTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAACCTGACTGACCTGTTGgaaga < 1:2651630/66‑1 (MQ=255)
cTGGTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAACCTGACTGACCTGTTGgaaga < 1:2657608/66‑1 (MQ=255)
cTGGTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAACCTGACTGACCTGTTGgaaga < 1:2679668/66‑1 (MQ=255)
cTGGTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAACCTGACTGACCTGTTGgaaga < 1:2750115/66‑1 (MQ=255)
cTGGTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAACCTGACTGACCTGTTGgaaga < 1:1830348/66‑1 (MQ=255)
cTGGTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAACCTGACTGACCTGTTGgaaga < 1:1789402/66‑1 (MQ=255)
cTGGTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAACCTGACTGACCTGTTGgaaga < 1:2295713/66‑1 (MQ=255)
cTGGTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAACCTGACTGACCTGTTGgaaga < 1:601001/66‑1 (MQ=255)
cTGGTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAACCTGACTGACCTGTTGgaaga < 1:1311655/66‑1 (MQ=255)
cTGGTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAACCTGACTGACCTGTTGgaaga < 1:1487049/66‑1 (MQ=255)
cTGGTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAACCTGACTGACCTGTTGgaaga < 1:58653/66‑1 (MQ=255)
cTGGTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAACCTGACTGACCAGTTGgaaga < 1:1666557/66‑1 (MQ=255)
tGGTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAATCTGACGGACCTGCTGGAAGAagag < 1:1828709/69‑1 (MQ=255)
tGGTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAATCTGACGGACCTGCTGGAAGAagag < 1:3044352/69‑1 (MQ=255)
tGGTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAATCTGACGGACCTGCTGGAAGAagag < 1:2704013/69‑1 (MQ=255)
tGGTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAATCTGACGGACCTGCTGGAAGAagag < 1:1488139/69‑1 (MQ=255)
tGGTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAATCTGACGGACCTGCTGGAAGAagag < 1:1587224/69‑1 (MQ=255)
tGGTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAATCTGACGGACCTGCTGGAAGAagag < 1:1142514/69‑1 (MQ=255)
tGGTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAATCTGACGGACCTGCTGGAAGAagag < 1:1070072/69‑1 (MQ=255)
tGGTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAATCTGACGGACCTGCTGGAAGAagag < 1:2248760/69‑1 (MQ=255)
tGGTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAATCTGACGGACCTGCTGGAAGAagag < 1:1033383/69‑1 (MQ=255)
aTCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAATCTGACGGACCTGCTGGAAGAAGAGATc < 1:1083770/68‑1 (MQ=255)
aTCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAATCTGACGGACCTGCTGGAAGAAGAGATc < 1:783350/68‑1 (MQ=255)
aTCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAATCTGACGGACCTGCTGGAAGAAGAGATc < 1:242886/68‑1 (MQ=255)
aGGATGTACAGGATTCGTTGAAGGCTTATGACATCAATCTGACGGACCTGCTGGAAGAAGAGATCGAcc > 1:3033157/1‑69 (MQ=255)
tGTACAGGATTCGTTGAAGGCTTATGACATCAATCTGACGGACCTGCTGGAAGAAGAGATCGACCCGgc < 1:2882794/69‑1 (MQ=255)
tGTACAGGATTCGTTGAAGGCTTATGACATCAATCTGACGGACCTGCTGGAAGAAGAGATCGACCCGgc < 1:1021846/69‑1 (MQ=255)
tGTACAGGATTCGTTGAAGGCTTATGACATCAATCTGACGGACCTGCTGGAAGAAGAGATCGACCCGgc < 1:1009967/69‑1 (MQ=255)
tGTACAGGATTCGTTGAAGGCTTATGACATCAATCTGACGGACCTGCTGGAAGAAGAGATCGACCCGgc < 1:2085300/69‑1 (MQ=255)
aGGCTTATGACATCAATCTGACGGACCTGCTGGAAGAAGAGATCGACCCGgc < 1:749194/52‑1 (MQ=255)
cTTATGGCATCAATCTGACGGACCTGCTGgaagaa > 1:442157/1‑35 (MQ=255)
cTTATGACATCAATCTGACGGACCTGCTGgaagaa > 1:2561809/1‑35 (MQ=255)
cTTATGACATCAATCTGACGGACCTGCTGgaagaa > 1:3010370/1‑35 (MQ=255)
cTTATGACATCAATCTGACGGACCTGCTGgaagaa > 1:2971602/1‑35 (MQ=255)
cTTATGACATCAATCTGACGGACCTGCTGgaagaa > 1:3075874/1‑35 (MQ=255)
cTTATGACATCAATCTGACGGACCTGCTGgaagaa > 1:1191208/1‑35 (MQ=255)
cTTATGACATCAATCTGACGGACCTGCTGgaagaa > 1:1067194/1‑35 (MQ=255)
cTTATGACATCAATCTGACGGACCTGCTGgaagaa > 1:1842175/1‑35 (MQ=255)
cTTATGACATCAATCTGACGGACCTGCTGgaagaa > 1:1915253/1‑35 (MQ=255)
cTTATGACATCAATCTGACGGACCTGCTGgaagaa > 1:1947373/1‑35 (MQ=255)
cTTATGACATCAATCTGACGGACCTGCTGgaagaa > 1:2052079/1‑35 (MQ=255)
cTTATGACATCAATCTGACGGACCTGCTGgaagaa > 1:2541689/1‑35 (MQ=255)
cTTATGACATCAATCTGACGGACCTGCTGgaagaa > 1:2521904/1‑35 (MQ=255)
cTTATGACATCAATCTGACGGACCTGCTGgaagaa > 1:2510908/1‑35 (MQ=255)
cTTATGACATCAATCTGACGGACCTGCTGgaagaa > 1:2435648/1‑35 (MQ=255)
cTTATGACATCAATCTGACGGACCTGCTGgaagaa > 1:1053360/1‑35 (MQ=255)
cTTATGACATCAATCTGACGGACCTGCTGgaagaa > 1:893122/1‑35 (MQ=255)
cTTATGACATCAATCTGACGGACCTGCTGgaagaa > 1:142268/1‑35 (MQ=255)
cTTATGACATCAATCTGACGGACCTGCTGGAAGAAGAGATCGACCCGGCGCTGGGTAACGGTGGTCTgg < 1:1696829/69‑1 (MQ=255)
cTTATGACATCAATCTGACGGACCTGCTGGAAGAAGAGATCGACCCGGCGCTGGGTAACGGTGGTCTgg < 1:2440514/69‑1 (MQ=255)
cTTATGACATCAATCTGACGGACCTGCTGGAAGAAGAGATCGACCCGGCGCTGGGTAACGGTGGTCTgg < 1:2984545/69‑1 (MQ=255)
cTTATGACATCAATCTGACGGACCTGCTCgaagaa > 1:756918/1‑35 (MQ=255)
tCAACCTGACTGACCTGTTGGAAGAAGAGATCGACCCGGCGCt > 1:518066/1‑43 (MQ=37)
tCAACCTGACTGACCTGTTGGAAGAAGAGATCGACCCGGCGCTGGGGAACgg > 1:3399072/1‑52 (MQ=255)
tCAACCTGACTGACCTGTTGGAAGAAGAGATCGACCCGGCGCTGGGGAACGGTGGTCTgg > 1:394976/1‑60 (MQ=255)
tCAACCTGACTGACCTGTTGGAAGAAGAGATCGACCCGGCGCTGGGGAACGGTGGTCTGGGACGTCTgg > 1:1327124/1‑69 (MQ=38)
tCAACCTGACTGACCTGTTGGAAGAAGAGATCGACCCGGCGCTGGGGAACGGTGGTCTGGGACGTCTgg > 1:775628/1‑69 (MQ=38)
tCAACCTGACTGACCTGTTGGAAGAAGAGATCGACCCGGCGCTGGGGAACGGTGGTCTGGGACGTCTgg > 1:2305251/1‑69 (MQ=38)
tCAACCTGACTGACCTGTTGGAAGAAGAGATCGACCCGGCGCTGGGGAACGGTGGTCTGGGACGTCTgg > 1:908233/1‑69 (MQ=38)
tCAACCTGACTGACCTGTTGGAAGAAGAGATCGACCCGGCGCTGGGGAACGGTGGTCTGGGACGTCTgg > 1:415492/1‑69 (MQ=38)
tCAACCTGACTGACCTGTTGGAAGAAGAGATCGACCCGGCGCTGGGGAACGGTGGTCTGGGACGTCTgg > 1:3302335/1‑69 (MQ=38)
tCAACCTGACTGACCTGTTGGAAGAAGAGATCGACCCGGCGCTGGGGAACGGTGGTCTGGGACGTCTgg > 1:146719/1‑69 (MQ=38)
tCAACCTGACTGACCTGTTGGAAGAAGAGATCGACCCGGCGCTGGGGAACGGTGGTCTGGGACGTCTgg > 1:1727355/1‑69 (MQ=38)
tCAACCTGACTGACCTGTTGGAAGAAGAGATCGACCCGGCGCTGGGGAACGGTGGTCTGGGACGTCTgg > 1:2788214/1‑69 (MQ=38)
tCAACCTGACTGACCTGTTGGAAGAAGAGATCGACCCGGCGCTGGGGAACGGTGGTCTGGGACGTCTgg > 1:2774253/1‑69 (MQ=38)
tCAACCTGACTGACCTGTTGGAAGAAGAGATCGACCCGGCGCTGGGGAACGGTGGTCTGGGACGTCTgg > 1:2396242/1‑69 (MQ=38)
tCAACCTGACTGACCTGTTGGAAGAAGAGATCGACCCGGCGCTGGGGAACGGTGGTCTGGGACGTCTgg > 1:228868/1‑69 (MQ=38)
tCAACCTGACTGACCTGTTGGAAGAAGAGATCGACCCGGCGCTGGGGAACGGTGGTCTGGGACGTCTgg > 1:2316240/1‑69 (MQ=38)
tCAACCTGACTGACCTGTTGGAAGAAGAGATCGACCCGGCGCTGGGGAACGGTGGTCTGGGACGTCTg > 1:2032967/1‑68 (MQ=255)
cTGACGGACCTGCTGGAAGAAGAGATCGACCCGGCGCTGGGTAACGGTGGTCt > 1:2093861/1‑53 (MQ=255)
cTGACGGACCTGCTGGAAGAAGAGATCGACCCGGCGCTGGGTAACGGTGGTCTGGGACGTCTggcggc > 1:2080996/1‑68 (MQ=255)
cTGACGGACCTGCTGGAAGAAGAGATCGACCCGGCGCTGGGTAACGGTGGACTGGGACGTCTgg > 1:268265/1‑64 (MQ=255)
|
GAATCTCGGCTGGTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAATCTGACGGACCTGCTGGAAGAAGAGATCGACCCGGCGCTGGGTAACGGTGGTCTGGGACGTCTGGCGGC > minE/2668862‑2668984
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
