Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,669,963 |
C→T |
36.2% |
N447N (AAC→AAT) |
malP → |
maltodextrin phosphorylase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,669,963 | 0 | C | T | 36.2%
| 60.5
/ 53.5
| 91 | N447N (AAC→AAT) | malP | maltodextrin phosphorylase |
| Reads supporting (aligned to +/- strand): ref base C (4/54); new base T (9/24); total (13/78) |
| Fisher's exact test for biased strand distribution p-value = 1.18e-02 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.09e-01 |
GGTGAAAGATCTGTTCCCGGAATATCACCAGCTATGGCCGAACAAATTCCATAACGTCACCAACGGTATTACCCCACGTCGCTGGATCAAACAGTGCAACCCGGCACTGGCGGCTCTGTTGG > minE/2669909‑2670030
|
ggtgAAAGATCTGTTCCCGGAATATCACCAGCTATGGCCGAACAAATTCCATAATGTCACCAACgg < 1:994992/66‑1 (MQ=255)
ggtgAAAGATCTGTTCCCGGAATATCACCAGCTATGGCCGAACAAATTCCATAATGTCACCAACgg < 1:2362719/66‑1 (MQ=255)
ggtgAAAGATCTGTTCCCGGAATATCACCAGCTATGGCCGAACAAATTCCATAATGTCACCAACgg < 1:2654918/66‑1 (MQ=255)
ggtgAAAGATCTGTTCCCGGAATATCACCAGCTATGGCCGAACAAATTCCATAATGTCACCAACgg < 1:1910151/66‑1 (MQ=255)
ggtgAAAGATCTGTTCCCGGAATATCACCAGCTATGGCCGAACAAATTCCATAATGTCACCAACgg < 1:1909871/66‑1 (MQ=255)
ggtgAAAGATCTGTTCCCGGAATATCACCAGCTATGGCCGAACAAATTCCATAATGTCACCAACgg < 1:1891700/66‑1 (MQ=255)
ggtgAAAGATCTGTTCCCGGAATATCACCAGCTATGGCCGAACAAATTCCATAATGTCACCAACgg < 1:2898169/66‑1 (MQ=255)
ggtgAAAGATCTGTTCCCGGAATATCACCAGCTATGGCCGAACAAATTCCATAATGTCACCAACgg < 1:1720891/66‑1 (MQ=255)
ggtgAAAGATCTGTTCCCGGAATATCACCAGCTATGGCCGAACAAATTCCATAATGTCACCAACgg < 1:3263959/66‑1 (MQ=255)
ggtgAAAGATCTGTTCCCGGAATATCACCAGCTATGGCCGAACAAATTCCATAATGTCACCAACgg < 1:350799/66‑1 (MQ=255)
ggtgAAAGATCTGTTCCCGGAATATCACCAGCTATGGCCGAACAAATTCCATAATGTCACCAACgg < 1:373200/66‑1 (MQ=255)
ggtgAAAGATCTGTTCCCGGAATATCACCAGCTATGGCCGAACAAATTCCATAATGTCACCAACgg < 1:726395/66‑1 (MQ=255)
ggtgAAAGATCTGTTCCCGGAATATCACCAGCTATGGCCGAACAAATTCCATAATGTCACCAACgg < 1:1257060/66‑1 (MQ=255)
ggtgAAAGATCTGTTCCCGGAATATCACCAGCTATGGCCGAACAAATTCCATAATGTCACCAACgg < 1:784114/66‑1 (MQ=255)
ggtgAAAGATCTGTTCCCGGAATATCACCAGCTATGGCCGAACAAATTCCATAATGTCACCAACgg < 1:1221557/66‑1 (MQ=255)
ggtgAAAGATCTGTTCCCGGAATATCACCAGCTATGGCCGAACAAATTCCATAATGTCACCAACgg < 1:1141057/66‑1 (MQ=255)
ggtgAAAGATCTGTTCCCGGAATATCACCAGCTATGGCCGAACAAATTCCATAATGTCACCAACgg < 1:752749/66‑1 (MQ=255)
ggtgAAAGATCTGTTCCCGGAATATCACCAGCTATGGCCGAACAAATTCCATAATGTCACCAACgg < 1:1362787/66‑1 (MQ=255)
ggtgAAAGATCTGTTCCCGGAATATCACCAGCTATGGCCGAACAAATTCCATAATGTCACCAACgg < 1:1204452/66‑1 (MQ=255)
ggtgAAAGATCTGTTCCCGGAATATCACCAGCTATGGCCGAACAAATTCCATAACGTCACCAACgg < 1:3131060/66‑1 (MQ=255)
ggtgAAAGATCTGTTCCCGGAATATCACCAGCTATGGCCGAACAAATTCCATAACGTCACCAACgg < 1:2184058/66‑1 (MQ=255)
ggtgAAAGATCTGTTCCCGGAATATCACCAGCTATGGCCGAACAAATTCCATAACGTCACCAACgg < 1:2167519/66‑1 (MQ=255)
ggtgAAAGATCTGTTCCCGGAATATCACCAGCTATGGCCGAACAAATTCCATAACGTCACCAACgg < 1:835481/66‑1 (MQ=255)
ggtgAAAGATCTGTTCCCGGAATATCACCAGCTATGGCCGAACAAATTCCATAACGTCACCAACgg < 1:1988558/66‑1 (MQ=255)
ggtgAAAGATCTGTTCCCGGAATATCACCAGCTATGGCCGAACAAATTCCATAACGTCACCAACgg < 1:2669164/66‑1 (MQ=255)
ggtgAAAGATCTGTTCCCGGAATATCACCAGCTATGGCCGAACAAATTCCATAACGTCACCAACgg < 1:2967355/66‑1 (MQ=255)
ggtgAAAGATCTGTTCCCGGAATATCACCAGCTATGGCCGAACAAATTCCATAACGTCACCAACgg < 1:3227574/66‑1 (MQ=255)
ggtgAAAGATCTGTTCCCGGAATATCACCAGCTATGGCCGAACAAATTCCATAACGTCACCAACgg < 1:1671419/66‑1 (MQ=255)
ggtgAAAGATCTGTTCCCGGAATATCACCAGCTATGGCCGAACAAATTCCATAACGTCACCAACgg < 1:370148/66‑1 (MQ=255)
ggtgAAAGATCTGTTCCCGGAATATCACCAGCTATGGCCGAACAAATTCCATAACGTCACCAACgg < 1:1512101/66‑1 (MQ=255)
ggtgAAAGATCTGTTCCCGGAATATCACCAGCTATGGCCGAACAAATTCCATAACGTCACCAACgg < 1:593534/66‑1 (MQ=255)
ggtgAAAGATCTGTTCCCGGAATATCACCAGCTATGGCCGAACAAATTCCATAACGTCACCAACgg < 1:674264/66‑1 (MQ=255)
ggtgAAAGATCTGTTCCCGGAATATCACCAGCTATGGCCGAACAAATTCCATAACGTCACCAACgg < 1:1271789/66‑1 (MQ=255)
gtgAAAGATCTGTTCCCGGAATATCACCAGCTATGGCCGAACAAATTCCATAACGTCACCAACgg < 1:2074687/65‑1 (MQ=255)
gAAAGATCTGTTCCCGGAATATCACCAGCTATGGCCGAACAAATTCCATAATGTCACCAACGGTATTa > 1:801642/1‑68 (MQ=255)
gAAAGATCTGTTCCCGGAATATCACCAGCTATGGCCGAACAAATTCCATAATGTCACCAACGGTATTa > 1:2570120/1‑68 (MQ=255)
gAAAGATCTGTTCCCGGAATATCACCAGCTATGGCCGAACAAATTCCATAATGTCACCAACGGTATTAc > 1:3306644/1‑69 (MQ=255)
gAAAGATCTGTTCCCGGAATATCACCAGCTATGGCCGAACAAATTCCATAATGTCACCAACGGTATTAc > 1:82977/1‑69 (MQ=255)
gAAAGATCTGTTCCCGGAATATCACCAGCTATGGCCGAACAAATTCCATAATGTCACCAACGGTATTAc > 1:560271/1‑69 (MQ=255)
gAAAGATCTGTTCCCGGAATATCACCAGCTATGGCCGAACAAATTCCATAATGTCACCAACGGTATTAc > 1:1060802/1‑69 (MQ=255)
gAAAGATCTGTTCCCGGAATATCACCAGCTATGGCCGAACAAATTCCATAATGTCACCAACGGTATTAc > 1:1070110/1‑69 (MQ=255)
gAAAGATCTGTTCCCGGAATATCACCAGCTATGGCCGAACAAATTCCATAATGTCACCAACGGTATTAc > 1:1837314/1‑69 (MQ=255)
gAAAGATCTGTTCCCGGAATATCACCAGCTATGGCCGAACAAATTCCATAATGTCACCAACGGTATTAc > 1:1212268/1‑69 (MQ=255)
aGATCTGTTCCCGGAATATCACCAGCTATGGCCGAACAAATTCCATAATGTCACCAACGGTATTAccc < 1:1530810/68‑1 (MQ=255)
aGATCTGTTCCCGGAATATCACCAGCTATGGCCGAACAAATTCCATAATGTCACCAACGGTATTAccc < 1:890409/68‑1 (MQ=255)
aGATCTGTTCCCGGAATATCACCAGCTATGGCCGAACAAATTCCATAATGTCACCAACGGTATTAccc < 1:2588136/68‑1 (MQ=255)
aGATCTGTTCCCGGAATATCACCAGCTATGGCCGAACAAATTCCATAATGTCACCAACGGTATTAccc < 1:2610328/68‑1 (MQ=255)
tGTTCCCGGAATATCACCAGCTATGGCCGAACAAATTCCATAACGTCACCAACGGTATTACCCCACGt > 1:952935/1‑68 (MQ=255)
tGTTCCCGGAATATCACCAGCTATGGCCGAACAAATTCCATAACGTCACCAACGGTATTACCCCACGTc > 1:1940984/1‑69 (MQ=255)
cccGGAATATCACCAGCTATGGCCGAACAAATTCCATAACGTCACCAACgg < 1:2859528/51‑1 (MQ=255)
cccGGAATATCACCAGCTATGGCCGAACAAATTCCATAACGTCACCAACgg < 1:361553/51‑1 (MQ=255)
cccGGAATATCACCAGCTATGGCCGAACAAATTCCATAACGTCACCAACgg < 1:1519438/51‑1 (MQ=255)
cccGGAATATCACCAGCTATGGCCGAACAAATTCCATAACGTCACCAACgg < 1:1654046/51‑1 (MQ=255)
cccGGAATATCACCAGCTATGGCCGAACAAATTCCATAACGTCACCAACgg < 1:523620/51‑1 (MQ=255)
cccGGAATATCACCAGCTATGGCCGAACAAATTCCATAACGTCACCAACgg < 1:1724389/51‑1 (MQ=255)
cccGGAATATCACCAGCTATGGCCGAACAAATTCCATAACGTCACCAACgg < 1:742155/51‑1 (MQ=255)
cccGGAATATCACCAGCTATGGCCGAACAAATTCCATAACGTCACCAACgg < 1:2788821/51‑1 (MQ=255)
cccGGAATATCACCAGCTATGGCCGAACAAATTCCATAACGTCACCAACgg < 1:1135337/51‑1 (MQ=255)
cccGGAATATCACCAGCTATGGCCGAACAAATTCCATAACGTCACCAACgg < 1:2164944/51‑1 (MQ=255)
cccGGAATATCACCAGCTATGGCCGAACAAATTCCATAACGTCACCAACgg < 1:2517669/51‑1 (MQ=255)
cccGGAATATCACCAGCTATGGCCGAACAAATTCCATAACGTCACCAACgg < 1:848745/51‑1 (MQ=255)
cccGGAATATCACCAGCTATGGCCGAACAAATTCCATAACGTCACCAACgg < 1:2186278/51‑1 (MQ=255)
cccGGAATATCACCAGCTATGGCAGAACAAATTCCATAACGTCACCAACgg < 1:1132867/51‑1 (MQ=255)
atatCACCAGCTATGGCCGAACAAATTCCATAACGTCACCAACGGTATTACCCCACGTCGCTGGATCa > 1:2178392/1‑68 (MQ=255)
atCACCAGCTATGGCCGAACAAATTCCATAACGTCACCAACGGTATTACCCCACGTCGCTGGATCAAAc < 1:2264209/69‑1 (MQ=255)
atCACCAGCTATGGCCGAACAAATTCCATAACGTCACCAACGGTATTACCCCACGTCGCTGGATCAAAc < 1:1403871/69‑1 (MQ=255)
atCACCAGCTATGGCCGAACAAATTCCATAACGTCACCAACGGTATTACCCCACGTCGCTGGATCAAAc < 1:1428477/69‑1 (MQ=255)
atCACCAGCTATGGCCGAACAAATTCCATAACGTCACCAACGGTATTACCCCACGTCGCTGGATCAAAc < 1:650464/69‑1 (MQ=255)
atCACCAGCTATGGCCGAACAAATTCCATAACGTCACCAACGGTATTACCCCACGTCGCTGGATCAAAc < 1:380413/69‑1 (MQ=255)
atCACCAGCTATGGCCGAACAAATTCCATAACGTCACCAACGGTATTACCCCACGTCGCTGGATCAAAc < 1:1490512/69‑1 (MQ=255)
atCACCAGCTATGGCCGAACAAATTCCATAACGTCACCAACGGTATTACCCCACGTCGCTGGATCAAAc < 1:1581784/69‑1 (MQ=255)
atCACCAGCTATGGCCGAACAAATTCCATAACGTCACCAACGGTATTACCCCACGTCGCTGGATCAAAc < 1:2296410/69‑1 (MQ=255)
atCACCAGCTATGGCCGAACAAATTCCATAACGTCACCAACGGTATTACCCCACGTCGCTGGATCAAAc < 1:2379056/69‑1 (MQ=255)
atCACCAGCTATGGCCGAACAAATTCCATAACGTCACCAACGGTATTACCCCACGTCGCTGGATCAAAc < 1:320222/69‑1 (MQ=255)
tCACCAGCTATGGCCGAACAAATTCCATAACGTCACCAACGGTATTACCCCACGTCGCTGGATCa < 1:2469502/65‑1 (MQ=255)
aGCTATGGCCGATCTAATTCCATAACGTCACCAACTGTATTACCCCACGTCGCTGGATCATAc < 1:98030/63‑1 (MQ=38)
aGCTATGGCCGAACAAATTCCATAACGTCACCAACgg < 1:2893139/37‑1 (MQ=255)
tATGGCCGAACAAATTCCATAATGTCACCAACGGTATTAccc < 1:3294823/42‑1 (MQ=255)
tATGGCCGAACAAATTCCATAACGTCACCAACGGTATTACCCCACGTCGCTGGATCAAACAGTGCAAcc > 1:1441688/1‑69 (MQ=255)
gCCGAACAAATTCCATAACGTCACCAACGGTATTACCCCACGTCGCTGGATCAAACAGTGCAACCCgg < 1:1456142/68‑1 (MQ=255)
gCCGAACAAATTCCATAACGTCACCAACGGTATTACCCCACGTCGCTGGATCAAACAGTGCAACCCgg < 1:1689453/68‑1 (MQ=255)
gCCGAACAAATTCCATAACGTCACCAACGGTATTACCCCACGTCGCTGGATCAAACAGTGCAACCCgg < 1:3038885/68‑1 (MQ=255)
gCCGAACAAATTCCATAACGTCACCAACGGTATTACCCCACGTCGCTGGATCAAACAGTGCAACCCgg < 1:3353649/68‑1 (MQ=255)
gCCGAACAAATTCCATAACGTCACCAACGGTATTACCCCACGTCGCTGGATCAAACAGTGCAACCCgg < 1:2893197/68‑1 (MQ=255)
gCCGAACAAATTCCATAACGTCACCAACGGTATTACCCCACGTCGCTGGATCAAACAGTGCAACCCgg < 1:1997646/68‑1 (MQ=255)
gCCGAACAAATTCCATAACGTCACCAACGGTATTACCCCACGTCGCTGGATCAAACAGTGCAACCCgg < 1:2625883/68‑1 (MQ=255)
gCCGAACAAATTCCATAACGTCACCAACGGTATTACCCCACGTCGCTGGATCAAACAGTGCAACCCgg < 1:1101230/68‑1 (MQ=255)
gCCGAACAAATTCCATAACGTCACCAACGGTATTACCCCACGTCGCTGGATCAAACAGTGCAACCCgg < 1:2395014/68‑1 (MQ=255)
gCCGAACAAATTCCATAACGTCACCAACGGTATTACCCCACGTCGCTGGATCAAACAGTGCAACCCgg < 1:947378/68‑1 (MQ=255)
gCCGAACAAATTCCATAACGTCACCAACGGTATTAACCCACGTCGCTGGATCAAACAGTGCAACCCgg < 1:1005888/68‑1 (MQ=255)
aCGTCACCAACGGTATTACCCCACGTCGCTGGATCAAACAGTGCAACCCGGCACTGGCGGCTCTGTTgg < 1:1846636/69‑1 (MQ=255)
cGTCACCAACGGTATTACCCCACGTCGCTGGATCAAACAGTGCAACCCgg < 1:768636/50‑1 (MQ=255)
|
GGTGAAAGATCTGTTCCCGGAATATCACCAGCTATGGCCGAACAAATTCCATAACGTCACCAACGGTATTACCCCACGTCGCTGGATCAAACAGTGCAACCCGGCACTGGCGGCTCTGTTGG > minE/2669909‑2670030
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
