Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,678,154 |
C→T |
32.4% |
R72H (CGC→CAC) |
yhgG ← |
predicted DNA‑binding transcriptional regulator |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,678,154 | 0 | C | T | 32.4%
| 33.7
/ 18.8
| 43 | R72H (CGC→CAC) | yhgG | predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base C (15/14); new base T (11/3); total (26/17) |
| Fisher's exact test for biased strand distribution p-value = 1.11e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GCAGACGCATACCGGGCTTTCCAAAACAGGCGATGGAGTAAGGTTAACGCAGCGCCCACCACTCGCGCAGACAGGCTTTTCCTTCCGGGCAGCTTTTACAACTGCCAGAGAGGCAGCCGTCAGGTTCTTCC > minE/2678089‑2678219
|
gcatacgcatacCGGGCTTTCCAAAACAGGCGATGGAGTAAGGTTAACGCAGCGCCCACCACTCGCGCa < 1:2986968/69‑1 (MQ=255)
ccGGGCTTTCCAAAACAGGCGATGGAGTAAGGTTAACGCAGCGCCCACCACTCGCGCAGACAGGCtttt > 1:1014108/1‑69 (MQ=255)
ccGGGCTTTCCAAAACAGGCGATGGAGTAAGGTTAACGCAGCGCCCACCACTCGCGCAGACAGGCtttt > 1:2247546/1‑69 (MQ=255)
ccGGGCTTTCCAAAACAGGCGATGGAGTAAGGTTAACGCAGCGCCCACCACTCGCGCAGACAGGCtttt > 1:516585/1‑69 (MQ=255)
ccGGGCTTTCCAAAACAGGCGATGGAGTAAGGTTAACGCAGCGCCCACCACTCGCGCAGACAGGCtttt > 1:174986/1‑69 (MQ=255)
ccGGGCTTTCCAAAACAGGCGATGGAGTAAGGTTAACGCAGCGCCCACCACTCGCGCAGACAGGCtttt > 1:2855182/1‑69 (MQ=255)
ccGGGCTTTCAAAAACAGGCGATGGAGTAAGGTTAACGCAGCGCCCACCACTCGCGCAGACAGGCtttt > 1:3267492/1‑69 (MQ=255)
gATGGAGTAAGGTTAACGCAGCGCCCACCACTCGCGCAGACAGGCTTTTCGTTCCGGGCAGCTTTTACa < 1:731708/69‑1 (MQ=255)
gATGGAGTAAGGTTAACGCAGCGCCCACCACTCGCGCAGACAGGCTTTTCCTTCCGGGCAGCTTTTAc < 1:496814/68‑1 (MQ=255)
aTGGAGTAAGGTTAACGCAGCGCCCACCACTCGCGCAGACAGGCTTTTCCTTCCGGGCAGCTTTTACa < 1:2750171/68‑1 (MQ=255)
ggAGTAAGGTTAACGCAGCGCCCACCACTCGCGCAGACAGGCTTTTCCTTCCGGGCAGCTTTTACAACt > 1:2242415/1‑69 (MQ=255)
ggAGTAAGGTTAACGCAGCGCCCACCACTCGCGCAGACAGGCTTTTCCTTCCGGGCAGCTTTTACAACt > 1:2646622/1‑69 (MQ=255)
ggAGTAAGGTTAACGCAGCGCCCACCACTCGCGCAGACAGGCTTTTCCTTCCGGGCAGCTTTTACAACt > 1:1440479/1‑69 (MQ=255)
ggAGTAAGGTTAACGCAGCGCCCACCACTCGCGCAGACAGGCTTTTCCTTCCGGGCAGCTTTTACAACt > 1:2903479/1‑69 (MQ=255)
ggAGTAAGGTTAACGCAGCGCCAACCACTCGCGCAGACAGGCTTTTCCTTCCGGGCAGCTTTTACAACt > 1:1667119/1‑69 (MQ=255)
acaaGGTTAACGCAACGCCCACCATTCGTGCAGACAGGCTTTTCCTTCCGGGCAGCTTTTACa < 1:1748666/61‑1 (MQ=255)
gTTAACGCAGCGCCCACCACTCGCGCAGACAGGCTTTTCCTTCCGGGCAGCTTTTACAACTGCCagaga < 1:1912111/69‑1 (MQ=255)
cGCAGCGCCCACCACTCGCGCAGACAGGCTTTTCCTTCCGGGCAGCTTTTACAACTGCCAGAGAGGCa < 1:1982602/68‑1 (MQ=255)
cGCAGCGCCCACCACTCGCGCAGACAGGCTTTTCCTTCCGGGCAGCTTTTACAACTGCCAGAGAGGCa < 1:1391221/68‑1 (MQ=255)
cGCAGCGCCCACCACTCGCGCAGACAGGCTTTTCCTTCCGGGCAGCTTTTACAACTGCCAGAGAGGCa < 1:2533736/68‑1 (MQ=255)
cGCAGCGCCCACCACTCGCGCAGACAGGCTTTTCCTTCCGGGCAGCTTTTACAACTGCCAGAGAGGCa < 1:2272794/68‑1 (MQ=255)
cGCAGCGCCCACCACTCGCGCAGACAGGCTTTTCCTTCCGGGCAGCTTTTACAACTGCCAGAGAGGCa < 1:2458205/68‑1 (MQ=255)
gCAGCGCCCACCACTCGCGCAGACAGGCTTTTCCTTCCGGGCAGCTTTTACAACTGCCAGAGAGGCAg < 1:2605822/68‑1 (MQ=255)
gCAGCGCCCACCACTCGCGCAGACAGGCTTTTCCTTCCGGGCAGCTTTTACAACTGCCAGAGAGGCAg < 1:62915/68‑1 (MQ=255)
gCAGCGCCCACCACTCGCGCAGACAGGCTTTTCCTTCCGGGCAGCTTTTACAACTGCCAGAGAGGCAg < 1:1146367/68‑1 (MQ=255)
cAGCGCCCACCACTCGCGCAGACAGGCTTTTCCTTCCGGGCAGCTTTTACAACTGCCAGAGAGGCAg < 1:934917/67‑1 (MQ=255)
caccatTCGTGCAGACAGGCTTTTCCTTCCGGGCAGCTTTTACa < 1:1677965/44‑1 (MQ=255)
ccatTCGTGCAGACAGGCTTTTCCTTCCGGGCAGCTTTTACAACTGCCGGAAAGACAGCCGTCAGGttc > 1:1648573/1‑69 (MQ=255)
ccatTCGTGCAGACAGGCTTTTCCTTCCGGGCAGCTTTTACAACTGCCGGAAAGACAGCCGTCAGGttc > 1:1012278/1‑69 (MQ=255)
ccatTCGTGCAGACAGGCTTTTCCTTCCGGGCAGCTTTTACAACTGCCGGAAAGACAGCCGTCAGGttc > 1:44399/1‑69 (MQ=255)
ccatTCGTGCAGACAGGCTTTTCCTTCCGGGCAGCTTTTACAACTGCCGGAAAGACAGCCGTCAGGttc > 1:1531479/1‑69 (MQ=255)
ccatTCGTGCAGACAGGCTTTTCCTTCCGGGCAGCTTTTACAACTGCCGGAAAGACAGCCGTCAGGttc > 1:3057468/1‑69 (MQ=255)
ccatTCGTGCAGACAGGCTTTTCCTTCCGGGCAGCTTTTACAACTGCCGGAAAGACAGCCGTCAGGttc > 1:2974815/1‑69 (MQ=255)
ccatTCGTGCAGACAGGCTTTTCCTTCCGGGCAGCTTTTACAACTGCCGGAAAGACAGCCGTCAGGttc > 1:1787666/1‑69 (MQ=255)
ccatTCGTGCAGACAGGCTTTTCCTTCCGGGCAGCTTTTACAACTGCCGGAAAGACAGCCGTCAGGttc > 1:2610973/1‑69 (MQ=255)
ccatTCGTGCAGACAGGCTTTTCCTTCCGGGCAGCTTTTACAACTGCCGGAAAGACAGCCGTCAGGttc > 1:2128505/1‑69 (MQ=255)
ccatTCGTGCAGACAGGCTTTTCCTTCCGGGCAGCTTTTACAACTGCCGGAAAGACAGCCGTCAGGttc > 1:2556088/1‑69 (MQ=255)
ccatTCGTGCAGACAGGCTTTTCCTTCCGGGCAGCTTTTACAACTGCCGGAAAGACAGCCGTCAGGt > 1:123333/1‑67 (MQ=255)
cacTCGCGCAGACAGGCTTTTCCTTCCGGGCAGCttt > 1:2750875/1‑37 (MQ=255)
cacTCGCGCAGACAGGCTTTTCCTTCCGGGCAGCttt > 1:2085270/1‑37 (MQ=255)
cacTCGCGCAGACAGGCTTTTCCTTCCGGGCAGCttt > 1:545883/1‑37 (MQ=255)
cacTCGCGCAGACAGGCTTTTCCTTCCGGGCAGCttt > 1:1323011/1‑37 (MQ=255)
attCGTGCAGACAGGCTTTTCCTTCCGGGCAGCTTTTACa < 1:1469789/38‑1 (MQ=255)
cgcgCAGACAGGCTTTTCCTTCCGGGCAGCTTTTACAACTGCCAGAGAGGCAGCCGTCAGGttcttc > 1:1879144/1‑67 (MQ=255)
cgcgCAGACAGGCTTTTCCTTCCGGGCAGCTTTTACAACTGCCAGAGAGGCAGCCGTCAGGttcttc > 1:3233767/1‑67 (MQ=255)
cgcgCAGACAGGCTTTTCCTTCCGGGCAGCTTTTACAACTGCCAGAGAGGCAGCCGTCAGGTTCTTcc > 1:2881261/1‑68 (MQ=255)
cgcgCAGACAGGCTTTTCCTTCCGGGCAGCTTTTACAACTGCCAGAGAGGCAGCCGTCAGGTTCTTcc > 1:2349912/1‑68 (MQ=255)
cgcgCAGACAGGCTTTTCCTTCCGGGCAGCTTTTACAACTGCCAGAGAGGCAGCCGTCAGGTTCTTcc > 1:1435469/1‑68 (MQ=255)
cgcgCAGACAGGCTTTTCCTTCCGGGCAGCTTTTACAACTGCCAGAGAGGCAGCCGTCAGGTTCTTcc > 1:912507/1‑68 (MQ=255)
cgcgCAGACAGGCTTTTCCTTCCGGGCAGCTTTTACAACTGCCAGAGAGGCAGCCGTCAGGTTCTTcc > 1:2144839/1‑68 (MQ=255)
|
GCAGACGCATACCGGGCTTTCCAAAACAGGCGATGGAGTAAGGTTAACGCAGCGCCCACCACTCGCGCAGACAGGCTTTTCCTTCCGGGCAGCTTTTACAACTGCCAGAGAGGCAGCCGTCAGGTTCTTCC > minE/2678089‑2678219
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
