Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,678,875 |
T→C |
23.9% |
A605A (GCA→GCG) |
feoB ← |
fused ferrous iron transporter, protein B |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,678,875 | 0 | T | C | 23.9%
| 92.9
/ 23.7
| 75 | A605A (GCA→GCG) | feoB | fused ferrous iron transporter, protein B |
| Reads supporting (aligned to +/- strand): ref base T (31/26); new base C (15/3); total (46/29) |
| Fisher's exact test for biased strand distribution p-value = 4.99e-02 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TGTCTTTCAGGCTCTGCCAGGTTTCATCTATCGCACTGAACAGCTCTTCACCGAGGTTAAATTCTGCCGGATTGAACTCTTCGTCCTGAATATTTTCTGCGGTGTAGAGGGTGTTGAGCGTACCCAC > minE/2678811‑2678937
|
tgtCTTTCAGGCTCTGCCAGGTTTCATCTATCGCACTGAACAGCTCTTCACCGAGGTTAAATTCTGCCg > 1:2505346/1‑69 (MQ=255)
tgtCTTTCAGGCTCTGCCAGGTTTCATCTATCGCACTGAACAGCTCTTCACCGAGGTTAAATTCTGCCg > 1:1631564/1‑69 (MQ=255)
gtCTTTCAGGCTCTGCCAGGTTTCATCTATCGCACTGAACAGCTCTTCACCGAGGTTAAATTCTGCCgg > 1:908493/1‑69 (MQ=255)
gtCTTTCAGGCTCTGCCAGGTTTCATCTATCGCACTGAACAGCTCTTCACCGAGGTTAAATTCTGCCgg > 1:61548/1‑69 (MQ=255)
gtCTTTCAGGCTCTGCCAGGTTTCATCTATCGCACTGAAAAGCTCTTCACCGAGGTTAAATTCTGCCgg > 1:1608703/1‑69 (MQ=255)
tttCAGGCTCTGCCAGGTTTCATCTATCGCACTGAACAGCTCTTCACCGAGGTTAAATTCTGCCGGAt > 1:829156/1‑68 (MQ=255)
tttCAGGCTCTGCCAGGTTTCATCTATCGCACTGAACAGCTCTTCACCGAGGTTAAATTCTGCCGGAt > 1:1840923/1‑68 (MQ=255)
tttCAGGCTCTGCCAGGTTTCATCTATCGCACTGAACAGCTCTTCACCGAGGTTAAATTCTGCCGGAt > 1:257038/1‑68 (MQ=255)
aGGCTCTGCCAGGTTTCATCTATCGCACTGAACAGCTCTTCACCGAGGTTAAATTCTGCCGGATTGaa < 1:2252989/68‑1 (MQ=255)
aGGCTCTGCCAGGTTTCATCTATCGCACTGAACAGCTCTTCACCGAGGTTAAATTCTGCCGGATTGaa < 1:775809/68‑1 (MQ=255)
ctctGCCAGGTTTCATCTATCGCACTGAACAGCTCTTCACCGAGGTTAAATTCTGCCGGATTGAActct > 1:289461/1‑69 (MQ=255)
tGCCAGGTTTCATCTATCGCACTGAACAGCTCTTCACCGAGGTTAAATTCTGcag < 1:3079173/55‑3 (MQ=255)
tGCCAGGTTTCATCTATCGCACTGAACAGCTCTTCACCGAGGTTAAATTCTGcag < 1:3264546/55‑3 (MQ=255)
tGCCAGGTTTCATCTATCGCACTGAACAGCTCTTCACCGAGGTTAAATTCTGCCg > 1:3417416/1‑55 (MQ=255)
tGCCAGGTTTCATCTATCGCACTGAACAGCTCTTCACCGAGGTTAAATTCTGCCg > 1:656256/1‑55 (MQ=255)
aTCTACCGCACTGAACAGCTCTTCACCGAGGTTAAATTCCGCCGGATTg > 1:3015792/1‑49 (MQ=38)
aTCTACCGCACTGAACAGCTCTTCACCGAGGTTAAATTCCGCCGGATTg > 1:2760117/1‑49 (MQ=38)
aTCTACCGCACTGAACAGCTCTTCACCGAGGTTAAATTCCGCCGGATTg > 1:248948/1‑49 (MQ=38)
aTCTACCGCACTGAACAGCTCTTCACCGAGGTTAAATTCCGCCGGATTg > 1:1271805/1‑49 (MQ=38)
aTCTACCGCACTGAACAGCTCTTCACCGAGGTTAAATTCCGCCGGATTg > 1:2394555/1‑49 (MQ=38)
aTCTACCGCACTGAACAGCTCTTCACCGAGGTTAAATTCCGCCGGATTg > 1:2380382/1‑49 (MQ=38)
aTCTACCGCACTGAACAGCTCTTCACCGAGGTTAAATTCCGCCGGATTg > 1:2554774/1‑49 (MQ=38)
aTCTACCGCACTGAACAGCTCTTCACCGAGGTTAAATTCCGCCGGATTg > 1:3245218/1‑49 (MQ=38)
aTCTACCGCACTGAACAGCTCTTCACCGAGGTTAAATTCCGCCGGATTg > 1:81861/1‑49 (MQ=38)
aTCTACCGCACTGAACAGCTCTTCACCGAGGTTAAATTCCGCCGGATTg > 1:2879922/1‑49 (MQ=38)
aTCTACCGCACTGAACAGCTCTTCACCGAGGTTAAATTCCGCCGGATTg > 1:1443741/1‑49 (MQ=38)
aTCTACCGCACTGAACAGCTCTTCACCGAGGTTAAATTCCGCCGGATTg > 1:3298207/1‑49 (MQ=38)
aTCTACCGCACTGAACAGCTCTTCACCGAGGTTAAATTCCGCCGGATTg > 1:1915493/1‑49 (MQ=38)
aTCTACCGCACTGAACAGCTCTTCACCGAGGTTAAATTCCGCCGGATTg > 1:179694/1‑49 (MQ=38)
aTCTACCGCACTGAACAGCTCTTCACAGAGGTTAAATTACGCCGGATTg > 1:3351751/1‑49 (MQ=255)
aCTGAACAGCTCTTCACCGAGGTTAAATTCTGCCGGATTGAACTCTTCGTCCTGAATATTTTCTGCgg < 1:1469646/68‑1 (MQ=255)
aCTGAACAGCTCTTCACCGAGGTTAAATTCTGCCGGATTGAACTCTTCGTCCTGAATATTTTCTGCgg < 1:3021121/68‑1 (MQ=255)
aCTGAACAGCTCTTCACCGAGGTTAAATTCTGCCGGATTGAACTCTTCGTCCTGAATATTTGCTGCgg < 1:442721/68‑1 (MQ=255)
gAACAGCTCTTCACCGAGGTTAAATTCTGCCGGATTGAACTCTTCGTCCTGAATATTTTCTGCGgtg > 1:2783528/1‑67 (MQ=255)
gAACAGCTCTTCACCGAGGTTAAATTCTGCCGGATTGAACTCTTCGTCCTGAATATTTTCTGCGgtg > 1:1603186/1‑67 (MQ=255)
gAACAGCTCTTCACCGAGGTTAAATTCTGCCGGATTGAACTCTTCGTCCTGAATATTTTCTGCGGTGTa < 1:3187166/69‑1 (MQ=255)
gAACAGCTCTTCACCGAGGTTAAATTCTGCCGGATTGAACTCTTCGTCCTGAATATTTTCTGCGGTGTa < 1:2218099/69‑1 (MQ=255)
gAACAGCTCTTCACCGAGGTTAAATTCTGCCGGATTGAACTCTTCGTCCTGAATATTTTCTGCGGTGTa < 1:445059/69‑1 (MQ=255)
gAACAGCTCTTCACCGAGGTTAAATTCTGCCGGATTGAACTCTTCGTCCTGAATATTTTCTGCGGTGTa < 1:991276/69‑1 (MQ=255)
aCAGCTCTTCACCGAGGTTAAATTCTGCCGGATTGAACTCTTCGTCCTGAATATTTTCTGCGGTGTa < 1:435153/67‑1 (MQ=255)
aGCTCTTCACCGAGGTTAAATTCTGCCGGATTGAACTCTTCGTCCTGAATATTTTCTGCGGTGTagag < 1:2576217/68‑1 (MQ=255)
ctctTCACCGAGGTTAAATTCTGCCGGATTGAACTCTTCGTCCTGAATATTTTCTGCGGTGTAGAGGgt > 1:1425884/1‑69 (MQ=255)
ctctTCACCGAGGTTAAATTCTGCCGGATTGAACTCTTCGTCCTGAATATTTTCTGCGGTGTAGAGGgt > 1:2942041/1‑69 (MQ=255)
ctTCACCGAGGTTAAATTCTGCCGGATTGAACTCTTCGTCCTGAata > 1:1724474/1‑47 (MQ=255)
ctTCACCGAGGTTAAATTCTGCCGGATTGAACTCTTCGTCCTGAata > 1:1439063/1‑47 (MQ=255)
ctTCACCGAGGTTAAATTCTGCCGGATTGAACTCTTCGTCCTGAata > 1:2946998/1‑47 (MQ=255)
gAGGTTAAATTCTGCCGGATTGAACTCTTCGTCCTGAATATTTTCTGCGGTGTAGAGGGTGTTGAGCGt < 1:1208058/69‑1 (MQ=255)
gAGGTTAAATTCTGCCGGATTGAACTCTTCGTCCTGAATATTTTCTGCGGTGTAGAGGGTGTTGAGCGt < 1:1283044/69‑1 (MQ=255)
gAGGTTAAATTCTGCCGGATTGAACTCTTCGTCCTGAATATTTTCTGCGGTGTAGAGGGTGTTGAGCGt < 1:3011870/69‑1 (MQ=255)
gAGGTTAAATTCTGCCGGATTGAACTCTTCGTCCTGAATATTTTCTGCGGTGTAGAGGGTGTTGAGCGt < 1:1261904/69‑1 (MQ=255)
gAGGTTAAATTCTGCCGGATTGAACTCTTCGTCCTGAATATTTTCTGCGGTGTAGAGGGTGTTGAGCGt < 1:2430793/69‑1 (MQ=255)
gAGGTTAAATTCTGCCGGATTGAACTCTTCGTCCTGAATATTTTCTGCGGTGTAGAGGGTGTTGAGCGt < 1:2254562/69‑1 (MQ=255)
gAGGTTAAATTCTGCCGGATTGAACTCTTCGTCCTGAATATTTTCTGCGGTGTAGAGGGTGTTGAGCGt < 1:1521653/69‑1 (MQ=255)
gAGGTTAAATTCTGCCGGATTGAACTCTTCGTCCTGAATATTTTCTGCGGTGTAGAGGGTGTTGAGCGt < 1:1636737/69‑1 (MQ=255)
gAGGTTAAATTCTGCCGGATTGAACTCTTCGTCCTGAATATTTTCTGCGGTGTAGAGGGTGTTGAGCGt < 1:199324/69‑1 (MQ=255)
gTTAAATTCTGCCGGATTGAACTCTTCGTCCTGAATATTTTCTGCGGTGTAGAGGGTGTTGAGCGTAcc < 1:954986/69‑1 (MQ=255)
gTTAAATTCTGCCGGATTGAACTCTTCGTCCTGAATATTTTCTGCGGTGTAGAGGGTGTTGAGCGTAcc < 1:2272675/69‑1 (MQ=255)
gTTAAATTCTGCCGGATTGAACTCTTCGTCCTGAATATTTTCTGAGGTGTAGAGGGTGTTGAGCGTAcc < 1:2982356/69‑1 (MQ=255)
ttAAATTCTGCCGGATTGAACTCTTCGTCCTGAATATTTTCTGCgg < 1:2877201/46‑1 (MQ=255)
aaaTTCTGCCGGATTGAACTCTTCGTCCTGAATATTTTCTGCGGTGTAGAGGGTGTTGAGCGTAcc > 1:1965891/1‑66 (MQ=255)
aaaTTCTGCCGGATTGAACTCTTCGTCCTGAATATTTTCTGCGGTGTAGAGGGTGTTGAGCGTAcc > 1:1693724/1‑66 (MQ=255)
aaaTTCTGCCGGATTGAACTCTTCGTCCTGAATATTTTCTGCGGTGTAGAGGGTGTTGAGCGTAcc > 1:3246726/1‑66 (MQ=255)
aaaTTCTGCCGGATTGAACTCTTCGTCCTGAATATTTTCTGCGGTGTAGAGGGTGTTGAGCGTAcc > 1:511224/1‑66 (MQ=255)
aaaTTCTGCCGGATTGAACTCTTCGTCCTGAATATTTTCTGCGGTGTAGAGGGTGTTGAGCGTAcc > 1:526842/1‑66 (MQ=255)
aaaTTCTGCCGGATTGAACTCTTCGTCCTGAATATTTTCTGCGGTGTAGAGGGTGTTGAGCGTAcc > 1:1488287/1‑66 (MQ=255)
aaaTTCTGCCGGATTGAACTCTTCGTCCTGAATATTTTCTGCGGTGTAGAGGGTGTTGAGCGTAcc > 1:2475332/1‑66 (MQ=255)
aaaTTCTGCCGGATTGAACTCTTCGTCCTGAATATTTTCTGCGGTGTAGAGGGTGTTGAGCGTAcc > 1:1408624/1‑66 (MQ=255)
aaaTTCTGCCGGATTGAACTCTTCGTCCTGAATATTTTCTGCGGTGTAGAGGGTGTTGAGCGTAcc > 1:2976213/1‑66 (MQ=255)
aaaTTCTGCCGGATTGAACTCTTCGTCCTGAATATTTTCTGCGGTGTAGAGGGTGTTGAGCGTAcc > 1:2686618/1‑66 (MQ=255)
aaaTTCTGCCGGATTGAACTCTTCGTCCTGAATATTTTCTGCGGTGTAGAGGGTGTTGAGCGTAcc > 1:2807156/1‑66 (MQ=255)
aaaTTCTGCCGGATTGAACTCTTCGTCCTGAATATTTTCTGCGGTGTAGAGGGTGTTGAGCGTACCCAc > 1:3328463/1‑69 (MQ=255)
aaaTTCTGCCGGATTGAACTCTTCGTCCTGAATATTTTCTGCGGTGTAGAGGGTGTTGAGCGTACCCAc > 1:1501633/1‑69 (MQ=255)
aaaTTCCGCCGGATTGAACTCTTCGTCCTGAATATTTTCTGCGGTGTAGAGGGTGTTGAGCGTACCCAc < 1:2207901/69‑1 (MQ=255)
aaaTTCCGCCGGATTGAACTCTTCGTCCTGAATATTTTCTGCGGTGTAGAGGGTGTTGAGCGTACCCAc < 1:550128/69‑1 (MQ=255)
aaaTTCCGCCGGATTGAACTCTTCGTCCTGAATATTTTCTGCGGTGTAGAGGGTGTTGAGCGTACCCAc < 1:2555415/69‑1 (MQ=255)
|
TGTCTTTCAGGCTCTGCCAGGTTTCATCTATCGCACTGAACAGCTCTTCACCGAGGTTAAATTCTGCCGGATTGAACTCTTCGTCCTGAATATTTTCTGCGGTGTAGAGGGTGTTGAGCGTACCCAC > minE/2678811‑2678937
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
