Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,732,515 |
G→A |
30.3% |
L195L (CTG→CTA) |
yheO → |
predicted DNA‑binding transcriptional regulator |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,732,515 | 0 | G | A | 30.3%
| 102.7
/ 48.4
| 105 | L195L (CTG→CTA) | yheO | predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base G (27/46); new base A (8/24); total (35/70) |
| Fisher's exact test for biased strand distribution p-value = 2.67e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
Only 100 of 116 total aligned reads displayed.
ATCGAAGAAGTGAATGCCGATCGCAATGTTTCTAATAACGCCAAAAATCGTCAGATCGTGCTGAATCTCTACGAGAAAGGGATCTTCGATATTAAAGATGCGATCAACCAGGTTGCTGACCGCCTGAACAT > minE/2732453‑2732583
|
aTCGAAGAAGTGAATGCCGATCGCAATGTTTCTAATAACGCCAAGAATCGTCAGATCGTGCTGAAtctc > 1:1368236/1‑69 (MQ=255)
aTCGAAGAAGTGAATGCCGATCGCAATGTTTCTAATAACGCCAAAAATCGTCAGATCGTGCTGAAtctc > 1:1492544/1‑69 (MQ=255)
aTCGAAGAAGTGAATGCCGATCGCAATGTTTC‑AATAACGCCAAAAATCGTCAGATCGTGCTGAATctct > 1:2452032/1‑69 (MQ=255)
gaagaaGTGAATGCCGATCGCAATGTTTCTAATAACGCCAAAAATCGTCAGATCGTGCTAAATCTCTAc < 1:253441/69‑1 (MQ=255)
gaagaaGTGAATGCCGATCGCAATGTTTCTAATAACGCCAAAAATCGTCAGATCGTGCTAAATCTCTAc < 1:20812/69‑1 (MQ=255)
gaagaaGTGAATGCCGATCGCAATGTTTCTAATAACGCCAAAAATCGTCAGATCGTGCTAAATCTCTAc < 1:1542728/69‑1 (MQ=255)
gaagaaGTGAATGCCGATCGCAATGTTTCTAATAACGCCAAAAATCGTCAGATCGTGCTAAATCTCTAc < 1:287923/69‑1 (MQ=255)
gaagaaGTGAATGCCGATCGCAATGTTTCTAATAACGCCAAAAATCGTCAGATCGTGCTAAATCTCTAc < 1:3351040/69‑1 (MQ=255)
gaagaaGTGAATGCCGATCGCAATGTTTCTAATAACGCCAAAAATCGTCAGATCGTGCTAAATCTCTAc < 1:722556/69‑1 (MQ=255)
gaagaaGTGAATGCCGATCGCAATGTTTCTAATAACGCCAAAAATCGTCAGATCGTGCTAAATCTCTAc < 1:742931/69‑1 (MQ=255)
gaagaaGTGAATGCCGATCGCAATGTTTCTAATAACGCCAAAAATCGTCAGATCGTGCTAAATCTCTAc < 1:1235527/69‑1 (MQ=255)
gaagaaGTGAATGCCGATCGCAATGTTTCTAATAACGCCAAAAATCGTCAGATCGTGCTAAATCTCTAc < 1:3213163/69‑1 (MQ=255)
gaagaaGTGAATGCCGATCGCAATGTTTCTAATAACGCCAAAAATCGTCAGATCGTGCTAAATCTCTAc < 1:1000747/69‑1 (MQ=255)
gaagaaGTGAATGCCGAGCGCAATGTTTCTAATAACGCCAAAAATCGTCAGATCGTGCTGAATctct > 1:672848/1‑67 (MQ=255)
caagaagTGAATGCCGATCGCAATGTTTCTAATAACGCCAAAAATCGTCAGATCGTGCTAAATCTCTAc < 1:1040340/68‑1 (MQ=255)
gTGAATGCCGATCGCAATGTTTCTAATAACGCCAAAAATCGTCAGATCGTGCTGAATCTCTACGAGaaa > 1:3136748/1‑69 (MQ=255)
gTGAATGCCGATCGCAATGTTTCTAATAACGCCAAAAATCGTCAGATCGTGCTGAATCTCTACGAGaaa > 1:2989160/1‑69 (MQ=255)
gTGAATGCCGATCGCAATGTTTCTAATAACGCCAAAAATCGTCAGATCGTGCTGAATCTCTACGAGaaa > 1:3349975/1‑69 (MQ=255)
gTGAATGCCGATCGCAATGTTTCTAATAACGCCAAAAATCGTCAGATCGTGCTGAATCTCTACGAGaaa > 1:2544767/1‑69 (MQ=255)
gTGAATGCCGATCGCAATGTTTCTAATAACGCCAAAAATCGTCAGATCGTGCTGAATCTCTACGAGaaa > 1:1567844/1‑69 (MQ=255)
gTGAATGCCGATCGCAATGTTTCTAATAACGCCAAAAATCGTCAGATCGTGCTGAATCTCTACGAGaaa > 1:568456/1‑69 (MQ=255)
gTGAATGCCGATCGCAATGTTTCTAATAACGCCAAAAATCGTCAGATCGTGCTGAATCTCTACGAGaaa > 1:2099495/1‑69 (MQ=255)
gTGAATGCCGATCGCAATGTTTCTAATAACGCCAAAAATCGTCAGATCGTGCTGAATCTCTACGAGaaa > 1:678207/1‑69 (MQ=255)
gTGAATGCCGATCGCAATGTTTCTAATAACGCCAAAAATCGTCAGATCGTGCTGAATCTCTACGAGaaa > 1:1167294/1‑69 (MQ=255)
gTGAATGCCGATCGCAATGTTTCTAATAACGCCAAAAATCGTCAGATCGTGCTGAATCTCTACGAGaaa > 1:2449568/1‑69 (MQ=255)
tGCCGATCGCAATGTTTCTAATAACGCCAAAAATCGTCAGATCGTGCTGAATCTCTACGAGAAAGGGAt > 1:1662162/1‑69 (MQ=255)
tGCCGATCGCAATGTTTCTAATAACGCCAAAAATCGTCAGATCGTGCTAAATCTCTAc < 1:1948936/58‑1 (MQ=255)
tGCCGATCGCAATGTTTCTAATAACGCCAAAAATAGTCAGATCGTGCTGAATCTCTACGAGAAAggg > 1:693666/1‑67 (MQ=255)
tCGCAATGTTTCTAATAACGCCAAAAATCGTCAGATCGTGCTGAATCTCTACGAGAAAGGGATCTTCGa < 1:192317/69‑1 (MQ=255)
tCGCAATGTTTCTAATAACGCCAAAAATCGTCAGATCGTGCTGAATCTCTACGAGAAAGGGATCTTCGa < 1:3212359/69‑1 (MQ=255)
tCGCAATGTTTCTAATAACGCCAAAAATCGTCAGATCGTGCTGAATCTCTACGAGAAAGGGATCTTCGa < 1:3002509/69‑1 (MQ=255)
tCGCAATGTTTCTAATAACGCCAAAAATCGTCAGATCGTGCTGAATCTCTACGAGAAAGGGATCTTCGa < 1:3369950/69‑1 (MQ=255)
tCGCAATGTTTCTAATAACGCCAAAAATCGTCAGATCGTGCTGAATCTCTACGAGAAAGGGATCTTCGa < 1:2733208/69‑1 (MQ=255)
tCGCAATGTTTCTAATAACGCCAAAAATCGTCAGATCGTGCTGAATCTCTACGAGAAAGGGATCTTCGa < 1:483236/69‑1 (MQ=255)
tCGCAATGTTTCTAATAACGCCAAAAATCGTCAGATCGTGCTGAATCTCTACGAGAAAGGGATCTTCGa < 1:1477980/69‑1 (MQ=255)
tCGCAATGTTTCTAATAACGCCAAAAATCGTCAGATCGTGCTGAATCTCTACGAGAAAGGGATCTTCGa < 1:2140306/69‑1 (MQ=255)
tCGCAATGTTTCTAATAACGCCAAAAATCGTCAGATCGTGCTGAATCTCTACGAGAAAGGGATCTTCGa < 1:1185691/69‑1 (MQ=255)
tCGCAATGTTTCTAATAACGCCAAAAATCGTCAGATCGTGCTGAATCTCTACGAGAAAGGGATCTTCGa < 1:773553/69‑1 (MQ=255)
gTTTCTAATAACGCCAAAAATCGTCAGATCGTGCTGAATCTCTACGAGAAAGGGATCTTCGATATTaaa < 1:980828/69‑1 (MQ=255)
gTTTCTAATAACGCCAAAAATCGTCAGATCGTGCTGAATCTCTACGAGAAAGGGATCTTCGATATTaaa < 1:2779851/69‑1 (MQ=255)
gTTTCTAATAACGCCAAAAATCGTCAGATCGTGCTGAATCTCTACGAGAAAGGGATCTTCGATATTaaa < 1:345609/69‑1 (MQ=255)
gTTTCTAATAACGCCAAAAATCGTCAGATCGTGCTGAATCTCTACGAGAAAGGGATCTTCGATATTaaa < 1:2411313/69‑1 (MQ=255)
gTTTCTAATAACGCCAAAAATCGTCAGATCGTGCTGAATCTCTACGAGAAAGGGATCTTCGATATTaaa < 1:1909592/69‑1 (MQ=255)
tttCTAATAACGCCAAAAATCGTCAGATCGTGCTGAATCTCTACg < 1:1316907/45‑1 (MQ=255)
tttCTAATAACGCCAAAAATCGTCAGATCGTGCTGAATCTCTACg < 1:1479723/45‑1 (MQ=255)
tttCTAATAACGCCAAAAATCGTCAGATCGTGCTGAATCTCTACg < 1:488068/45‑1 (MQ=255)
tttCTAATAACGCCAAAAATCGTCAGATCGTGCTGAATCTCTACg < 1:1583629/45‑1 (MQ=255)
tttCTAATAACGCCAAAAATCGTCAGATCGTGCTGAATCTCTACg < 1:1683076/45‑1 (MQ=255)
tttCTAATAACGCCAAAAATCGTCAGATCGTGCTGAATCTCTACg < 1:1730005/45‑1 (MQ=255)
tttCTAATAACGCCAAAAATCGTCAGATCGTGCTGAATCTCTACg < 1:1939396/45‑1 (MQ=255)
tttCTAATAACGCCAAAAATCGTCAGATCGTGCTGAATCTCTACg < 1:945408/45‑1 (MQ=255)
tttCTAATAACGCCAAAAATCGTCAGATCGTGCTGAATCTCTACg < 1:970390/45‑1 (MQ=255)
tttCTAATAACGCCAAAAATCGTCAGATCGTGCTGAATCTCTACGAGAAAg > 1:2581179/1‑51 (MQ=255)
tttCTAATAACGCCAAAAATCGTCAGATCGTGCTGAATCTCTACGAGAAAGGGATCTTCGATATTAAAg > 1:1291483/1‑69 (MQ=255)
tttCTAATAACGCCAAAAATCGTCAGATCGTGCTGAATCTCTACGAGAAAGGGATCTTCGATATTAAAg > 1:1264206/1‑69 (MQ=255)
tttCTAATAACGCCAAAAATCGTCAGATCGTGCTGAATCTCTACGAGAAAGGGATCTTCGATATTAAAg > 1:1635128/1‑69 (MQ=255)
tttCTAATAACGCCAAAAATCGTCAGATCGTGCTGAATCTCTACGAGAAAGGGATCTTCGATATTAAAg > 1:323508/1‑69 (MQ=255)
tttCTAATAACGCCAAAAATCGTCAGATCGTGCTGAATCTCTACGAGAAAGGGATCTTCGATATTAAAg > 1:2057723/1‑69 (MQ=255)
tatCTAATAACGCCAAAAATCGTCAGATCGTGCTGAATCTCTACg < 1:2339908/43‑1 (MQ=255)
tCTAATAACGCCAAAAATCGTCAGATCGTGCTGAATCTCTACGAGAAAGGGATCTTCGATATTAAAGa < 1:2229772/68‑1 (MQ=255)
tCTAATAACGCCAAAAATCGTCAGATCGTGCTGAATCTCTACGAGAAAGGGATCTTCGATATTAAAGa < 1:6797/68‑1 (MQ=255)
tCTAATAACGCCAAAAATCGTCAGATCGTGCTGAATCTCTACGAGAAAGGGATCTTCGATATTAAAGa < 1:503499/68‑1 (MQ=255)
tCTAATAACGCCAAAAATCGTCAGATCGTGCTGAATCTCTACGAGAAAGGGATCTTCGATATTAAAGa < 1:3402384/68‑1 (MQ=255)
tCTAATAACGCCAAAAATCGTCAGATCGTGCTGAATCTCTACGAGAAAGGGATCTTCGATATTAAAGa < 1:1777454/68‑1 (MQ=255)
tCTAATAACGCCAAAAATCGTCAGATCGTGCTGAATCTCTACGAGAAAGGGATCTTCGATATTAAAGa < 1:874940/68‑1 (MQ=255)
tCTAATAACGCCAAAAATCGTCAGATCGTGCTGAATCTCTACGAGAAAGGGATCTTCGATATTAAAGa < 1:2663545/68‑1 (MQ=255)
aataaCGCCAAAAATCGTCAGATCGTGCTAAATCTCTACGAGAAAGGGATCTTCGATATTAAAGACGc < 1:2713546/68‑1 (MQ=255)
aataaCGCCAAAAATCGTCAGATCGTGCTAAATCTCTACGAGAAAGGGATCTTCGATATTAAAGACGc < 1:478519/68‑1 (MQ=255)
aataaCGCCAAAAATCGTCAGATCGTGCTAAATCTCTACGAGAAAGGGATCTTCGATATTAAAGACGc < 1:2278200/68‑1 (MQ=255)
aataaCGCCAAAAATCGTCAGATCGTGCTAAATCTCTACGAGAAAGGGATCTTCGATATTAAAGACGc < 1:2096732/68‑1 (MQ=255)
aataaCGCCAAAAATCGTCAGATCGTGCTAAATCTCTACGAGAAAGGGATCTTCGATATTAAAGACGc < 1:3211013/68‑1 (MQ=255)
aataaCGCCAAAAATCGTCAGATCGTGCTAAATCTCTACGAGAAAGGGATCTTCGATATTAAAGACGc < 1:2767352/68‑1 (MQ=255)
aataaCGCCAAAAATCGTCAGATCGTGCTAAATCTCTACGAGAAAGGGATCTTCGATATTAAAGACGc < 1:1963998/68‑1 (MQ=255)
aataaCGCCAAAAATCGTCAGATCGTGCTAAATCTCTACGAGAAAGGGATCTTCGATATTAAAGACGc < 1:2781526/68‑1 (MQ=255)
aaCGCCAAAAATCGTCAGATCGTGCTGAATCTCTACGAGAAAGGGATCTTCGATATTAAAGa < 1:2336972/62‑1 (MQ=255)
aaaTCGTCAGATCGTGCTAAATCTCTACGAGAAAGGGATCTTCGATATTaa > 1:580560/1‑51 (MQ=255)
aaaTCGTCAGATCGTGCTAAATCTCTACGAGAAAGGGATCTTCGATATTaa > 1:960064/1‑51 (MQ=255)
aaaTCGTCAGATCGTGCTAAATCTCTACGAGAAAGGGATCTTCGATATTaa > 1:2302749/1‑51 (MQ=255)
aaaTCGTCAGATCGTGCTAAATCTCTACGAGAAAGGGATCTTCGATATTaa > 1:2485283/1‑51 (MQ=255)
aaaTCGTCAGATCGTGCTAAATCTCTACGAGAAAGGGATCTTCGATATTaa > 1:474335/1‑51 (MQ=255)
aaaTCGTCAGATCGTGCTAAATCTCTACGAGAAAGGGATCTTCGATATTaa > 1:3224777/1‑51 (MQ=255)
aaaTCGTCAGATCGTGCTAAATCTCTACGAGAAAGGGATCTTCGATATTaa > 1:3033872/1‑51 (MQ=255)
gTCAGATCGTGCTGAATCTCTACGAGAAAGGGATCTTCGATATTAAAGATGCGATCAACCAGGTTGCTg < 1:820149/69‑1 (MQ=255)
cAGATCGTGCTGAATCTCTACGAGTAAGGGATCTTCGATATTAAAGa < 1:2692118/47‑1 (MQ=255)
aGATCGTGCTGAATCTCTACGAGAAAGGGATCTTCGa < 1:3198771/37‑1 (MQ=255)
gTGCTGAATCTCTACGAGAAAGGGATCTTCGATATTAAAGATGCGATCGACCAGGTTGCTGACCGCCTg < 1:3407222/69‑1 (MQ=255)
gTGCTGAATCTCTACGAGAAAGGGATCTTCGATATTAAAGATGCGATCAACCAGGTTGCTGACCGCCTg < 1:1096257/69‑1 (MQ=255)
gTGCTGAATCTCTACGAGAAAGGGATCTTCGATATTAAAGATGCGATCAACCAGGTTGCTGACCGCCTg < 1:727929/69‑1 (MQ=255)
gTGCTGAATCTCTACGAGAAAGGGATCTTCGATATTAAAGATGCGATCAACCAGGTTGCTGACCGCCTg < 1:1083846/69‑1 (MQ=255)
gTGCTGAATCTCTACGAGAAAGGGATCTTCGATATTAAAGATGCGATCAACCAGGTTGCTGACCGCCTg < 1:1966408/69‑1 (MQ=255)
gTGCTGAATCTCTACGAGAAAGGGATCTTCGATATTAAAGATGCGATCAACCAGGTTGCTGACCGCCTg < 1:2917721/69‑1 (MQ=255)
gAATCTCTACGAGAAAGGGATCTTCGATATTAAGGATGCGATCAACCAGGTTGCTGACCGCCTGAACAt < 1:1621060/69‑1 (MQ=255)
gAATCTCTACGAGAAAGGGATCTTCGATATTAAAGATGCGATCAACCAGGTTGCTGACCGCCTGAACAt < 1:1302904/69‑1 (MQ=255)
gAATCTCTACGAGAAAGGGATCTTCGATATTAAAGATGCGATCAACCAGGTTGCTGACCGCCTGAACAt < 1:1369039/69‑1 (MQ=255)
gAATCTCTACGAGAAAGGGATCTTCGATATTAAAGATGCGATCAACCAGGTTGCTGACCGCCTGAACAt < 1:446465/69‑1 (MQ=255)
gAATCTCTACGAGAAAGGGATCTTCGATATTAAAGATGCGATCAACCAGGTTGCTGACCGCCTGAACAt < 1:3357468/69‑1 (MQ=255)
gAATCTCTACGAGAAAGGGATCTTCGATATTAAAGATGCGATCAACCAGGTTGCTGACCGCCTGAACAt < 1:881113/69‑1 (MQ=255)
gAATCTCTACGAGAAAGGGATCTTCGATATTAAAGATGCGATCAACCAGGTTGCTGACCGCCTGAACAt < 1:965772/69‑1 (MQ=255)
gAATCTCTACGAGAAAGGGATCTTCGATATTAAAGATGCGATCAACCAGGTTGCTGACCGCCTGAACAt < 1:1969017/69‑1 (MQ=255)
gAATCTCTACGAGAAAGGGATCTTCGATATTAAAGATGCGATCAACCAGGTTGCTGACCGCCTGAACAt < 1:201973/69‑1 (MQ=255)
|
ATCGAAGAAGTGAATGCCGATCGCAATGTTTCTAATAACGCCAAAAATCGTCAGATCGTGCTGAATCTCTACGAGAAAGGGATCTTCGATATTAAAGATGCGATCAACCAGGTTGCTGACCGCCTGAACAT > minE/2732453‑2732583
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
