Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,732,742 |
T→A |
54.3% |
D30E (GAT→GAA) |
yheN → |
predicted intracellular sulfur oxidation protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,732,742 | 0 | T | A | 54.3%
| 11.2
/ 90.9
| 81 | D30E (GAT→GAA) | yheN | predicted intracellular sulfur oxidation protein |
| Reads supporting (aligned to +/- strand): ref base T (21/16); new base A (32/12); total (53/28) |
| Fisher's exact test for biased strand distribution p-value = 1.63e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.40e-01 |
GGGCCAGCATACGGTACGCAACAGGCGAGTAGTGCTTTTCAGTTTGCGCAGGCGCTGATAGCAGATGGCCATGAGTTAAGCAGCGTCTTTTTCTATCGGGAAGGGGTCTATAACGCTAACCA > minE/2732677‑2732798
|
gggCCAGCATACGGTACGCAACAGGCGAGTAGTGCTTTTCAGTTTGCGCAGGCGCTGATAGCAGATGGc < 1:1764119/69‑1 (MQ=255)
gggCCAGCATACGGTACGCAACAGGCGAGTAGTGCTTTTCAGTTTGCGCAGGCGCTGATAGCAGATGGc < 1:1679358/69‑1 (MQ=255)
gggCCAGCATACGGTACGCAACAGGCGAGTAGTGCTTTTCAGTTTGCGCAGGCGCTGATAGCAGATGGc < 1:678747/69‑1 (MQ=255)
gggCCAGCATACGGTACGCAACAGGCGAGTAGTGCTTTTCAGTTTGCGCAGGCGCTGATAGCAGATGGc < 1:443551/69‑1 (MQ=255)
ggCCAGCATACGGTACGCAACAGGCGAGTAGTGCTTTTCAGTTTGCGCAGGCGCTGATAGCAGATGGc < 1:1788753/68‑1 (MQ=255)
aTACGGTACGCAACAGGCGAGTAGTGCTTTTCAGTTTGCGCAGGCGCTGATAGCAGATGGCCATGa > 1:2459832/1‑66 (MQ=255)
aTACGGTACGCAACAGGCGAGTAGTGCTTTTCAGTTTGCGCAGGCGCTGATAGCAGATGGCCATGa > 1:1812264/1‑66 (MQ=255)
aTACGGTACGCAACAGGCGAGTAGTGCTTTTCAGTTTGCGCAGGCGCTGATAGCAGATGGCCATGAg > 1:3247148/1‑67 (MQ=255)
aTACGGTACGCAACAGGCGAGTAGTGCTTTTCAGTTTGCGCAGGCGCTGATAGCAGATGGCCATGAg > 1:2398075/1‑67 (MQ=255)
gTACGCAACAGGCGAGTAGTGCTTTTCAGTTTGCGCAGGCGCTAATAGCAGAAGGCCATGAGTTAagca < 1:586331/69‑1 (MQ=255)
gTACGCAACAGGCGAGTAGTGCTTTTCAGTTTGCGCAGGCGCTAATAGCAGAAGGCCATGAGTTAagca < 1:2450051/69‑1 (MQ=255)
gTACGCAACAGGCGAGTAGTGCTTTTCAGTTTGCGCAGGCGCTAATAGCAGAAGGCCATGAGTTAagca < 1:2955477/69‑1 (MQ=255)
gTACGCAACAGGCGAGTAGTGCTTTTCAGTTTGCGCAGGCGCTAATAGCAGAAGGCCATGAGTTAagca < 1:2775411/69‑1 (MQ=255)
gTACGCAACAGGCGAGTAGTGCTTTTCAGTTTGCGCAGGCGCTAATAGCAGAAGGCCATGAGTTAagca < 1:2693239/69‑1 (MQ=255)
gTACGCAACAGGCGAGTAGTGCTTTTCAGTTTGCGCAGGCGCTAATAGCAGAAGGCCATGAGTTAagca < 1:1733794/69‑1 (MQ=255)
gTACGCAACAGGCGAGTAGTGCTTTTCAGTTTGCGCAGGCGCTAATAGCAGAAGGCCATGAGTTAagca < 1:2470664/69‑1 (MQ=255)
gTACGCAACAGGCGAGTAGTGCTTTTCAGTTTGCGCAGGCGCTAATAGCAGAAGGCCATGAGTTAagca < 1:2074620/69‑1 (MQ=255)
gTACGCAACAGGCGAGTAGTGCTTTTCAGTTTGCGCAGGCGCTAATAGCAGAAGGCCATGAGTTAagca < 1:2100599/69‑1 (MQ=255)
gTACGCAACAGGCGAGTAGTGCTTTTCAGTTTGCGCAGGCGCTAATAGCAGAAGGCCATGAGTTAagca < 1:1147932/69‑1 (MQ=255)
gTACGCAACAGGCGAGTAGTGCTTTTCAGTTTGCGCAGGCGCTAATAGCAGAAGGCCATGAGTTAagca < 1:1827375/69‑1 (MQ=255)
gTACGCAACAGGCGAGTAGTGCTTTTCAGTTTGCGCAGGCGCTAATAGCAGAAGGCCATGAGTTAagca < 1:2186523/69‑1 (MQ=255)
aGGCGAGTAGTGCTTTTCAGTTTGCGCAGGCGCTAATAGCAGAAGGCCAt > 1:3408190/1‑50 (MQ=255)
aGGCGAGTAGTGCTTTTCAGTTTGCGCAGGCGCTAATAGCAGAAGGCCATGAGTTAagca > 1:96400/1‑60 (MQ=255)
aGGCGAGTAGTGCTTTTCAGTTTGCGCAGGCGCTAATAGCAGAAGGCCATGAGTTAAGCAGCGtttttt > 1:349938/1‑69 (MQ=255)
aGGCGAGTAGTGCTTTTCAGTTTGCGCAGGCGCTAATAGCAGAAGGCCATGAGTTAAGCAGCGtttttt > 1:2444399/1‑69 (MQ=255)
aGGCGAGTAGTGCTTTTCAGTTTGCGCAGGCGCTAATAGCAGAAGGCCATGAGTTAAGCAGCGtttttt > 1:689858/1‑69 (MQ=255)
aGGCGAGTAGTGCTTTTCAGTTTGCGCAGGCGCTAATAGCAGAAGGCCATGAGTTAAGCAGCGtttttt > 1:2332599/1‑69 (MQ=255)
aGGCGAGTAGTGCTTTTCAGTTTGCGCAGGCGCTAATAGCAGAAGGCCATGAGTTAAGCAGCGtttttt > 1:847621/1‑69 (MQ=255)
aGGCGAGTAGTGCTTTTCAGTTTGCGCAGGCGCTAATAGCAGAAGGCCATGAGTTAAGCAGCGtttttt > 1:2271545/1‑69 (MQ=255)
aGGCGAGTAGTGCTTTTCAGTTTGCGCAGGCGCTAATAGCAGAAGGCCATGAGTTAAGCAGCGtttttt > 1:889350/1‑69 (MQ=255)
aGGCGAGTAGTGCTTTTCAGTTTGCGCAGGCGCTAATAGCAGAAGGCCATGAGTTAAGCAGCGtttttt > 1:1128421/1‑69 (MQ=255)
aGGCGAGTAGTGCTTTTCAGTTTGCGCAGGCGCTAATAGCAGAAGGCCATGAGTTAAGCAGCGtttttt > 1:2156328/1‑69 (MQ=255)
aGGCGAGTAGTGCTTTTCAGTTTGCGCAGGCGCTAATAGCAGAAGGCCATGAGTTAAGCAGCGtttttt > 1:2150394/1‑69 (MQ=255)
aGGCGAGTAGTGCTTTTCAGTTTGCGCAGGCGCTAATAGCAGAAGGCCATGAGTTAAGCAGCGtttttt > 1:1662117/1‑69 (MQ=255)
aGGCGAGTAGTGCTTTTCAGTTTGCGCAGGCGCTAATAGCAGAAGGCCATGAGTTAAGCAGCGtttttt > 1:1056466/1‑69 (MQ=255)
aGGCGAGTAGTGCTTTTCAGTTTGCGCAGGCGCTAATAGCAGAAGGCCATGAGTTAAGCAGCGtttttt > 1:1937723/1‑69 (MQ=255)
aGGCGAGTAGTGCTTTTCAGTTTGCGCAGGCGCTAATAGCAGAAGGCCATGAGTTAAGCAGCGtttt > 1:2469163/1‑67 (MQ=255)
aGGCGAGTAGTGCTTTTCAGTTTGCGCAGGCGCTAATAGAAGAAGGCCATGAGTTAAGCAGCGtttttt > 1:896153/1‑69 (MQ=255)
cGAGTAGTGCTTTTCAGTTTGCGCAGGCGCTGATAGCAGATGGCCATGAGTTAAGCAGCGTCTTTTTCt < 1:1562301/69‑1 (MQ=255)
cGAGTAGTGCTTTTCAGTTTGCGCAGGCGCTGATAGCAGATGGCCATGAGTTAAGCAGCGTCTTTTTCt < 1:669122/69‑1 (MQ=255)
cGAGTAGTGCTTTTCAGTTTGCGCAGGCGCTGATAGCAGATGGCCATGAGTTAAGCAGCGTCTTTTTCt < 1:1412962/69‑1 (MQ=255)
cGAGTAGTGCTTTTCAGTTTGCGCAGGCGCTGATAGCAGATGGCCATGAGTTAAGCAGCGTCTTTTTCt < 1:738306/69‑1 (MQ=255)
cGAGTAGTGCTTTTCAGTTTGCGCAGGCGCTGATAGCAGATGGCCATGAGTTAAGCAGCGTCTTTTTCt < 1:825245/69‑1 (MQ=255)
cGAGTAGTGCTTTTCAGTTTGCGCAGGCGCTGATAGCAGATGGCCATGAGTTAAGCAGCGTCTTTTTCt < 1:2059184/69‑1 (MQ=255)
cGAGTAGTGCTTTTCAGTTTGCGCAGGCGCTGATAGCAGATGGCCATGAGTTAAGCAGCGTCTTTTTCt < 1:2214592/69‑1 (MQ=255)
gAGTAGTGCTTTTCAGTTTGCGCAGGCGCTGATAGCAGATGGCCATGAGTTAAGCAGCGTCTTTTTCt < 1:1738073/68‑1 (MQ=255)
tagtGCTTTTCAGTTTGCGCAGGCGCTGATAGCAGATGGCCATGAGTTAAGCAGCGTCTTTTTCTATc > 1:83268/1‑68 (MQ=255)
tagtGCTTTTCAGTTTGCGCAGGCGCTGATAGCAGATGGCCATGAGTTAAGCAGCGTCTTTTTCTATc > 1:1444800/1‑68 (MQ=255)
tagtGCTTTTCAGTTTGCGCAGGCGCTGATAGCAGATGGCCATGAGTTAAGCAGCGTCTTTTTCTATc > 1:3361876/1‑68 (MQ=255)
gtGCTTTTCAGTTTGCGCAGGCGCTGATAGCAGATGGCCATGAGTTAAGCAGCGTCTTTTTCTATc > 1:896478/1‑66 (MQ=255)
gtGCTTTTCAGTTTGCGCAGGCGCTGATAGCAGATGGCCATGAGTTAAGCAGCGTCTTTTTCTATc > 1:736403/1‑66 (MQ=255)
gtGCTTTTCAGTTTGCGCAGGCGCTGATAGCAGATGGCCATGAGTTAAGCAGCGTCTTTTTCTATc > 1:2961415/1‑66 (MQ=255)
gtGCTTTTCAGTTTGCGCAGGCGCTGATAGCAGATGGCCATGAGTTAAGCAGCGTCTTTTTCTATc > 1:604878/1‑66 (MQ=255)
gtGCTTTTCAGTTTGCGCAGGCGCTGATAGCAGATGGCCATGAGTTAAGCAGCGTCTTTTTCTATc > 1:473088/1‑66 (MQ=255)
gtGCTTTTCAGTTTGCGCAGGCGCTGATAGCAGATGGCCATGAGTTAAGCAGCGTCTTTTTCTATc > 1:1494815/1‑66 (MQ=255)
gtGCTTTTCAGTTTGCGCAGGCGCTGATAGCAGATGGCCATGAGTTAAGCAGCGTCTTTTTCTATc > 1:3345374/1‑66 (MQ=255)
gtGCTTTTCAGTTTGCGCAGGCGCTGATAGCAGATGGCCATGAGTTAAGCAGCGTCTTTTTCTATc > 1:1533597/1‑66 (MQ=255)
gCTTTTCAGTTTGCGCAGGCGCTGATAGCAGATGGCCATGAGTTAAGCAGCGTCTTTTTCTATCGGGaa > 1:1998609/1‑69 (MQ=255)
cTTTTCAGTTTGCGCAGGCGCTGATAGCAGATGGCCATGAGTTAAGCAGCGTCTTTTTCTATCGGGAAg > 1:1503190/1‑69 (MQ=255)
cTTTTCAGTTTGCGCAGGCGCTGATAGCAGATGGCCATGAGTTAAGCAGCGTCTTTTTCTATCGGGAAg > 1:3002264/1‑69 (MQ=255)
gTTTGCGCAGGCGCTGATAGCAGATGGCCATGAGTTAAGCAGCGTCTTTTTCTATCGGGAAGGGGTc > 1:975685/1‑67 (MQ=255)
gTTTGCGCAGGCGCTGATAGCAGATGGCCATGAGTTAAGCAGCGTCTTTTTCTATCGGGAAGGGGTCt > 1:1521455/1‑68 (MQ=255)
tttGCGCAGGCGCTGATAGCAGATGGCCATGAGTTAAGCAGCGTCTTTTTCTATCGGGAAGGGGTCtat < 1:2814800/69‑1 (MQ=255)
tttGCGCAGGCGCTGATAGCAGATGGCCATGAGTTAAGCAGCGTCTTTCTCTATCGGGAAGGGGTCtat < 1:320116/69‑1 (MQ=255)
tGCGCAGGCGCTAATAGCAGAAGGCCATGAGTTAAGCAGCGttt > 1:921910/1‑42 (MQ=255)
tGCGCAGGCGCTAATAGCAGAAGGCCATGAGTTAAGCAGCGttt > 1:1664600/1‑42 (MQ=255)
tGCGCAGGCGCTAATAGCAGAAGGCCATGAGTTAAGCAGCGttt > 1:209644/1‑42 (MQ=255)
tGCGCAGGCGCTAATAGCAGAAGGCCATGAGTTAAGCAGCGttt > 1:2288623/1‑42 (MQ=255)
tGCGCAGGCGCTAATAGCAGAAGGCCATGAGTTAAGCAGCGttt > 1:1165384/1‑42 (MQ=255)
tGCGCAGGCGCTAATAGCAGAAGGCCATGAGTTAAGCAGCGttt > 1:1246866/1‑42 (MQ=255)
tGCGCAGGCGCTAATAGCAGAAGGCCATGAGTTAAGCAGCGttt > 1:698278/1‑42 (MQ=255)
tGCGCAGGCGCTAATAGCAGAAGGCCATGAGTTAAGCAGCGttt > 1:130830/1‑42 (MQ=255)
tGCGCAGGCGCTAATAGCAGAAGGCCATGAGTTAAGCAGCGttt > 1:1926366/1‑42 (MQ=255)
tGCGCAGGCGCTAATAGCAGAAGGCCATGAGTTAAGCAGCGttt > 1:449442/1‑42 (MQ=255)
tGCGCAGGCGCTAATAGCAGAAGGCCATGAGTTAAGCAGCGttt > 1:406741/1‑42 (MQ=255)
tGCGCAGGCGCTAATAGCAGAAGGCCATGAGTTAAGCAGCGttt > 1:2156090/1‑42 (MQ=255)
tGCGCAGGCGCTAATAGCAGAAGGCCATGAGTTAAGCAGCGttt > 1:3286169/1‑42 (MQ=255)
tGCGCAGGCGCTAATAGCAGAAGGCCATGAGTTAAGCAGCGttt > 1:2993392/1‑42 (MQ=255)
tGCGCAGGCGCTAATAACAGAAGGCCATGAGTTAAGCAGCGttt > 1:1999057/1‑42 (MQ=255)
gcTGATAGCAGATGGCCATGAGTTAAGCAGCGTCTTTTTCTATCGGGAAGGGGTCTATAACGCTAACCa < 1:903156/69‑1 (MQ=255)
gATAGCAGATGGCCATGAGTTAAGCAGCGTCTTTTTCTATCGGGAAGGGGTCTATaa > 1:2427476/1‑57 (MQ=255)
|
GGGCCAGCATACGGTACGCAACAGGCGAGTAGTGCTTTTCAGTTTGCGCAGGCGCTGATAGCAGATGGCCATGAGTTAAGCAGCGTCTTTTTCTATCGGGAAGGGGTCTATAACGCTAACCA > minE/2732677‑2732798
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
