Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,794,549 |
A→G |
24.9% |
I264I (ATT→ATC) |
plsB ← |
glycerol‑3‑phosphate O‑acyltransferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,794,549 | 0 | A | G | 24.9%
| 82.9
/ 24.7
| 72 | I264I (ATT→ATC) | plsB | glycerol‑3‑phosphate O‑acyltransferase |
| Reads supporting (aligned to +/- strand): ref base A (27/27); new base G (12/6); total (39/33) |
| Fisher's exact test for biased strand distribution p-value = 2.79e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.20e-01 |
CGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTT > minE/2794482‑2794616
|
cGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGa < 1:2553430/68‑1 (MQ=255)
cGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGa < 1:63872/68‑1 (MQ=255)
aTGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAatcat > 1:2145913/1‑69 (MQ=255)
cGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGt < 1:2470354/69‑1 (MQ=255)
cGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGt < 1:2425977/69‑1 (MQ=255)
cGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGt < 1:1138805/69‑1 (MQ=255)
cGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGt < 1:815590/69‑1 (MQ=255)
cGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGt < 1:124792/69‑1 (MQ=255)
cGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGt < 1:2413908/69‑1 (MQ=255)
cGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGt < 1:2262579/69‑1 (MQ=255)
cGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGt < 1:1457447/69‑1 (MQ=255)
cGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGt < 1:3407683/69‑1 (MQ=255)
cGTTGATGCCCTGGTAAAGTCGGTTCCAGATGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGt < 1:954093/69‑1 (MQ=255)
ttGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGt < 1:1075547/67‑1 (MQ=255)
gCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAgaga < 1:2147960/68‑1 (MQ=255)
gCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAgaga < 1:3111888/68‑1 (MQ=255)
gCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAgaga < 1:41916/68‑1 (MQ=255)
tAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGaa > 1:1491695/1‑62 (MQ=255)
tAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTcgc > 1:1863487/1‑68 (MQ=255)
tAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTcgc > 1:1313854/1‑68 (MQ=255)
tAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTcgc > 1:3093979/1‑68 (MQ=255)
tAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTcgc > 1:3316383/1‑68 (MQ=255)
tAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTcgc > 1:2216407/1‑68 (MQ=255)
tAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTcgc > 1:762632/1‑68 (MQ=255)
aaaGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAGTCATCTCGTAAGAGAAATTagcc > 1:396035/1‑68 (MQ=255)
aaaGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTcgcc > 1:1283079/1‑68 (MQ=255)
aaaGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTcgcc > 1:2136913/1‑68 (MQ=255)
tCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAAt < 1:1878168/69‑1 (MQ=255)
tCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAAt < 1:255610/69‑1 (MQ=255)
tCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAAt < 1:251533/69‑1 (MQ=255)
ggTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGt < 1:2653467/48‑1 (MQ=255)
ggTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGt < 1:230474/41‑1 (MQ=255)
ggTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCAt < 1:3127829/69‑1 (MQ=255)
tGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCa > 1:370626/1‑69 (MQ=255)
tGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCa > 1:1823862/1‑69 (MQ=255)
tGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCa > 1:1544298/1‑69 (MQ=255)
aTACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATcgcg < 1:2493952/69‑1 (MQ=255)
aTACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATcgcg < 1:49226/69‑1 (MQ=255)
aTACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATcgcg > 1:3202963/1‑69 (MQ=255)
aTACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATcgcg < 1:1720096/69‑1 (MQ=255)
cGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTc > 1:82901/1‑69 (MQ=255)
cGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTc > 1:3182644/1‑69 (MQ=255)
cGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTc > 1:2514196/1‑69 (MQ=255)
cGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTc > 1:3266505/1‑69 (MQ=255)
cGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTc > 1:1748501/1‑69 (MQ=255)
tcagtcagGCGGATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTTCATCAGCGCAATCGCGTTctgc > 1:2470639/1‑69 (MQ=255)
tcagtcagGCGGATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCTCGTTctgc > 1:464302/1‑69 (MQ=255)
tcagtcagGCGGATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTctgc > 1:2990191/1‑69 (MQ=255)
tcagtcagGCGGATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTctgc > 1:100190/1‑69 (MQ=255)
tcagtcagGCGGATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTctgc > 1:2286494/1‑69 (MQ=255)
tcagtcagGCGGATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTctgc > 1:438512/1‑69 (MQ=255)
tcagtcagGCGGATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTctgc > 1:2257310/1‑69 (MQ=255)
tcagtcagGCGGATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTctgc > 1:2166920/1‑69 (MQ=255)
tcagtcagGCGGATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTctgc > 1:3386694/1‑69 (MQ=255)
tcagtcagGCGGATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTctgc > 1:1968414/1‑69 (MQ=255)
tcagtcagGCGGATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTctgc > 1:1925577/1‑69 (MQ=255)
tcagtaagGCGGATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATc > 1:1294529/1‑51 (MQ=255)
cagGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTgcgc > 1:1868386/1‑69 (MQ=255)
cagGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTgcgc > 1:1179197/1‑69 (MQ=255)
cagGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTgcgc > 1:733123/1‑69 (MQ=255)
cagGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTgcgc > 1:1867776/1‑69 (MQ=255)
cagGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTgcgc > 1:3222560/1‑69 (MQ=255)
cagGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTgcgc > 1:3166649/1‑69 (MQ=255)
cagGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTACATCAGTGCAATCGCGTTCTGCTgcgc > 1:2348052/1‑69 (MQ=255)
gCGGATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCtt < 1:1669036/68‑1 (MQ=255)
gCGGATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCtt < 1:595794/68‑1 (MQ=255)
gCGGATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCtt < 1:165557/68‑1 (MQ=255)
gCGGATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCtt < 1:71310/68‑1 (MQ=255)
gCGGATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCtt < 1:2503181/68‑1 (MQ=255)
gCGGATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCtt < 1:1019032/68‑1 (MQ=255)
gAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCttttt < 1:2856228/69‑1 (MQ=255)
gAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCttttt < 1:650296/69‑1 (MQ=255)
gAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCttttt < 1:266845/69‑1 (MQ=255)
gAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCttttt < 1:1057630/69‑1 (MQ=255)
|
CGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTT > minE/2794482‑2794616
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
