Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,803,445 |
T→C |
45.6% |
T86T (ACT→ACC) |
dnaB → |
replicative DNA helicase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,803,445 | 0 | T | C | 45.6%
| 10.4
/ 76.1
| 90 | T86T (ACT→ACC) | dnaB | replicative DNA helicase |
| Reads supporting (aligned to +/- strand): ref base T (19/30); new base C (17/24); total (36/54) |
| Fisher's exact test for biased strand distribution p-value = 8.32e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.28e-01 |
CACACCGTCATATCTTTACTGAAATGGCGCGTTTGCAGGAAAGCGGTAGCCCTATCGATCTGATTACTCTTGCGGAATCGCTGGAACGCCAGGGGCAACTCGATAGCGTCGGTGGTTTTGCTTATCTGGCAGA > minE/2803378‑2803510
|
cacaCCGTCATATCTTTACTGAAATGGCGCGTTTGCAGGAAAGCGGTAGCCCTATCGATCTGATTActc > 1:551182/1‑69 (MQ=255)
acCGTCATATCTTTACTGAAATGGCGCGTTTGCAGGAAAGCGGTAGTCCTATCGATCTGATTACCCtt > 1:1244934/1‑68 (MQ=255)
acCGTCATATCTTTACTGAAATGGCGCGTTTGCAGGAAAGCGGTAGTCCTATCGATCTGATTACCCTTg > 1:1300710/1‑69 (MQ=255)
acCGTCATATCTTTACTGAAATGGCGCGTTTGCAGGAAAGCGGTAGTCCTATCGATCTGATTACCCTTg > 1:1814971/1‑69 (MQ=255)
acCGTCATATCTTTACTGAAATGGCGCGTTTGCAGGAAAGCGGTAGTCCTATCGATCTGATTACCCTTg > 1:2078331/1‑69 (MQ=255)
acCGTCATATCTTTACTGAAATGGCGCGTTTGCAGGAAAGCGGTAGTCCTATCGATCTGATTACCCTTg > 1:248691/1‑69 (MQ=255)
acCGTCATATCTTTACTGAAATGGCGCGTTTGCAGGAAAGCGGTAGTCCTATCGATCTGATTACCCTTg > 1:2627235/1‑69 (MQ=255)
acCGTCATATCTTTACTGAAATGGCGCGTTTGCAGGAAAGCGGTAGTCCTATCGATCTGATTACCCTTg > 1:2706027/1‑69 (MQ=255)
acCGTCATATCTTTACTGAAATGGCGCGTTTGCAGGAAAGCGGTAGTCCTATCGATCTGATTACCCTTg > 1:1357075/1‑69 (MQ=255)
acCGTCATATCTTTACTGAAATGGCGCGTTTGCAGGAAAGCGGTAGTCCTATCGATCTGATTACCCTTg > 1:1338827/1‑69 (MQ=255)
acCGTCATATCTTTACTGAAATGGCGCGTTTGCAGGAAAGCGGTAGTCCTATCGATCTGATTACCCTTg > 1:3052250/1‑69 (MQ=255)
acCGTCATATCTTTACTGAAATGGCGCGTTTGCAGGAAAGCGGTAGTCCTATCGATCTGATTACCCTTg > 1:31330/1‑69 (MQ=255)
acCGTCATATCTTTACTGAAATGGCGCGTTTGCAGGAAAGCGGTAGTCCTATCGATCTGATTACCCTTg > 1:3396803/1‑69 (MQ=255)
acCGTCATATCTTTACTGAAATGGCGCGTTTGCAGGAAAGCGGTAGTCCTATCGATCTGATTACCCTTg > 1:3428960/1‑69 (MQ=255)
acCGTCATATCTTTACTGAAATGGCGCGTTTGCAGGAAAGCGGTAGTCCTATCGATCTGATTACCCTTg > 1:401958/1‑69 (MQ=255)
acCGTCATATCTTTACTGAAATGGCGCGTTTGCAGGAAAGCGGTAGTCCTATCGATCTGATTACCCTTg > 1:1184790/1‑69 (MQ=255)
acCGTCATATCTTTACTGAAATGGCGCGTTTGCAGGAAAGCGGTAGTCCTATCGATCTGATTACCCTTg > 1:614211/1‑69 (MQ=255)
acCGTCATATCTTTACTGAAATGGCGCGTTTGCAGGAAAGCGGTAGTCCTATCGATCTGATTACCCTTg > 1:1020280/1‑69 (MQ=255)
ccGTCATATCTTTACTGAAATGGCGCGTTTGCAGGAAAGCGGTAGCCCTATCGATCTGATTACTCTTg > 1:3034850/1‑68 (MQ=255)
ccGTCATATCTTTACTGAAATGGCGCGTTTGCAGGAAAGCGGTAGCCCTATCGATCTGATTACTCTTg > 1:2065100/1‑68 (MQ=255)
gTCATATCTTTACTGAAATGGCGCGTTTGCAGGAAAGCGGTAGCCCTATCGATCTGATTACTCTTGCgg > 1:960513/1‑69 (MQ=255)
tCATATCTTTACTGAAATGGCGCGTTTGCAGGAAAGCGGTAGCCCTATCGATCTGATTACTCTTGCGGa < 1:3112402/69‑1 (MQ=255)
tCATATCTTTACTGAAATGGCGCGTTTGCAGGAAAGCGGTAGCCCTATCGATCTGATTACTCTTGCGGa < 1:2684307/69‑1 (MQ=255)
tCATATCTTTACTGAAATGGCGCGTTTGCAGGAAAGCGGTAGCCCTATCGATCTGATTACTCTTGCGGa < 1:885139/69‑1 (MQ=255)
tCATATCTTTACTGAAATGGCGCGTTTGCAGGAAAGCGGTAGCCCTATCGATCTGATTACTCTTGCGGa < 1:2466577/69‑1 (MQ=255)
tCATATCTTTACTGAAATGGCGCGTTTGCAGGAAAGCGGTAGCCCTATCGATCTGATTACTCTTGCGGa < 1:2027805/69‑1 (MQ=255)
tCATATCTTTACTGAAATGGCGCGTTTGCAGGAAAGCGGTAGCCCTATCGATCTGATTACTCTAGCGGa < 1:2254475/69‑1 (MQ=255)
atCTTTACTGAAATGGCGCGTTTGCAGGAAAGCGGTAGCCCTATCGATCTGATTACTCTTGCGGAATc < 1:2884277/68‑1 (MQ=255)
atCTTTACTGAAATGGCGCGTTTGCAGGAAAGCGGTAGCCCTATCGATCTGATTACTCTTGCGGAATCg > 1:3251129/1‑69 (MQ=255)
tCTTTACTTAAATGGCGCGTTTGCAGGAAAGCGGTAGCCCTATCGATCTGATTACTCTTGCGGAATCGc > 1:390423/1‑69 (MQ=255)
tCTTTACTGAAATGGCGCGTTTGCAGGAAAGCGGTAGCCCTATCGATCTGATTACTCTTGCgg > 1:3142452/1‑63 (MQ=255)
tCTTTACTGAAATGGCGCGTTTGCAGGAAAGCGGTAGCCCTATCGATCTGATTACTCTTGCgg > 1:3348489/1‑63 (MQ=255)
tCTTTACTGAAATGGCGCGTTTGCAGGAAAGCGGTAGCCCTATCGATCTGATTACTCTTGCgg > 1:356790/1‑63 (MQ=255)
tCTTTACTGAAATGGCGCGTTTGCAGGAAAGCGGTAGCCCTATCGATCTGATTACTCTTGCgg > 1:186613/1‑63 (MQ=255)
tCTTTACTGAAATGGCGCGTTTGCAGGAAAGCGGTAGCCCTATCGATCTGATTACTCTTGCgg > 1:657031/1‑63 (MQ=255)
tCTTTACTGAAATGGCGCGTTTGCAGGAAAGCGGTAGCCCTATCGATCTGATTACTCTTGCGGAATCg > 1:1267321/1‑68 (MQ=255)
tCTTTACTGAAATGGCGCGTTTGCAGGAAAGCGGTAGCCCTATCGATCTGATTACTCTTGCGGAATCGc > 1:245036/1‑69 (MQ=255)
tCTTTACTGAAATGGCGCGTTTGCAGGAAAGCGGTAGCCCTATCGATCTGATTACTCTTGCGGAATCGc > 1:2451892/1‑69 (MQ=255)
tCTTTACTGAAATGGCGCGTTTGCAGGAAAGCGGTAGCCCTATCGATCTGATTACTCTTGCGGAATCGc > 1:2263738/1‑69 (MQ=255)
tCTTTACTGAAATGGCGCGTTTGCAGGAAAGCGGTAGCCCTATCGATCTGATTACTCTTGCGGAATCGc > 1:2673569/1‑69 (MQ=255)
tCTTTACTGAAATGGCGCGTTTGCAGGAAAGCGGTAGCCCTATCGATCTGATTACTCTTGCGGAATCGc > 1:1169247/1‑69 (MQ=255)
aTGGCGCGTTTGCAGGAAAGCGGTAGTCCTATCGATCTTATTACCCTTGCGGAATCGCTGGAACGCCa < 1:3269905/68‑1 (MQ=255)
aTGGCGCGTTTGCAGGAAAGCGGTAGTCCTATCGATCTGATTACCCTTGCGGAATCGCTGGAACGCCa < 1:1264739/68‑1 (MQ=255)
aTGGCGCGTTTGCAGGAAAGCGGTAGTCCTATCGATCTGATTACCCTTGCGGAATCGCTGGAACGCCa < 1:2857353/68‑1 (MQ=255)
aTGGCGCGTTTGCAGGAAAGCGGTAGTCCTATCGATCTGATTACCCTTGCGGAATCGCTGGAACGCCa < 1:279841/68‑1 (MQ=255)
aTGGCGCGTTTGCAGGAAAGCGGTAGTCCTATCGATCTGATTACCCTTGCGGAATCGCTGGAACGCCa < 1:2864139/68‑1 (MQ=255)
aTGGCGCGTTTGCAGGAAAGCGGTAGTCCTATCGATCTGATTACCCTTGCGGAATCGCTGGAACGCCa < 1:132900/68‑1 (MQ=255)
aTGGCGCGTTTGCAGGAAAGCGGTAGTCCTATCGATCTGATTACCCTTGCGGAATCGCTGGAACGCCa < 1:2771554/68‑1 (MQ=255)
aTGGCGCGTTTGCAGGAAAGCGGTAGTCCTATCGATCTGATTACCCTTGCGGAATCGCTGGAACGCCa < 1:315000/68‑1 (MQ=255)
aTGGCGCGTTTGCAGGAAAGCGGTAGTCCTATCGATCTGATTACCCTTGCGGAATCGCTGGAACGCCa < 1:337460/68‑1 (MQ=255)
aTGGCGCGTTTGCAGGAAAGCGGTAGTCCTATCGATCTGATTACCCTTGCGGAATCGCTGGAACGCCa < 1:2934170/68‑1 (MQ=255)
aTGGCGCGTTTGCAGGAAAGCGGTAGTCCTATCGATCTGATTACCCTTGCGGAATCGCTGGAACGCCa < 1:2363066/68‑1 (MQ=255)
aTGGCGCGTTTGCAGGAAAGCGGTAGTCCTATCGATCTGATTACCCTTGCGGAATCGCTGGAACGCCa < 1:1566619/68‑1 (MQ=255)
aTGGCGCGTTTGCAGGAAAGCGGTAGTCCTATCGATCTGATTACCCTTGCGGAATCGCTGGAACGCCa < 1:1533163/68‑1 (MQ=255)
aTGGCGCGTTTGCAGGAAAGCGGTAGTCCTATCGATCTGATTACCCTTGCGGAATCGCTGGAACGCCa < 1:650432/68‑1 (MQ=255)
aTGGCGCGTTTGCAGGAAAGCGGTAGTCCTATCGATCTGATTACCCTTGCGGAATCGCTGGAACGCCa < 1:1215531/68‑1 (MQ=255)
aTGGCGCGTTTGCAGGAAAGCGGTAGTCCTATCGATCTGATTACCCTTGCGGAATCGCTGGAACGCCa < 1:1251465/68‑1 (MQ=255)
aTGGCGCGTTTGCAGGAAAGCGGTAGTCCTATCGATCTGATTACCCTTGCGGAATCGCTGGAACGCCa < 1:2633081/68‑1 (MQ=255)
aTGGCGCGTTTGCAGGAAAGCGGTAGTCCTATCGATCTGATTACCCTTGCGGAATCGCTGGAACGCCa < 1:1423375/68‑1 (MQ=255)
aTGGCGCGTTTGCAGGAAAGCGGTAGTCCTATCGATCTGATTACCCTTGCGGAATCGCTGGAACGCCa < 1:2553773/68‑1 (MQ=255)
aTGGCGCGTTTGCAGGAAAGCGGTAGTCCTATCGATCTGATTACCCTTGCGGAATCGCTGGAACGCCa < 1:3421255/68‑1 (MQ=255)
aTGGCGCGTTTGCAGGAAAGCGGTAGTCCTATCGATCTGATTACCCTTGCGGAATCGCTGGAACGCCa < 1:1375952/68‑1 (MQ=255)
aTGGCGCGTTTGCAGGAAAGCGGTAGTCCAATCGATCTGATTACCCTTGCGGAATCGCTGGAACGCCa < 1:1426088/68‑1 (MQ=255)
tGGCGCGTTTGCAGGAAAGCGGTAGTCCTATCGATCTGATTACCCTTGCGGAATCGCTGGAACGCCa < 1:1280100/67‑1 (MQ=255)
tGGCGCGTTTGCAGGAAAGCGGTAGCCCTATCGATCTGATTACTCTTGCGGAATCGCTgg < 1:2631724/60‑1 (MQ=255)
ttGCAGGAAAGCGGTAGCCCTATCGATCTGATTACTCTTGCGGAATCGCTGGAACGCCAGGGGCAACTc < 1:3190555/69‑1 (MQ=255)
ttGCAGGAAAGCGGTAGCCCTATCGATCTGATTACTCTTGCGGAATCGCTGGAACGCCAGGGGCAACTc < 1:1012141/69‑1 (MQ=255)
ttGCAGGAAAGCGGTAGCCCTATCGATCTGATTACTCTTGCGGAATCGCTGGAACGCCAGGGGCAACTc < 1:76406/69‑1 (MQ=255)
ttGCAGGAAAGCGGTAGCCCTATCGATCTGATTACTCTTGCGGAATCGCTGGAACGCCAGGGGCAACTc < 1:1633636/69‑1 (MQ=255)
ttGCAGGAAAGCGGTAGCCCTATCGATCTGATTACTCTTGCGGAATCGCTGGAACGCCAGGGGCAACTc < 1:148451/69‑1 (MQ=255)
tGCAGGAAAGCGGTAGCCCTATCGATGTGATTACTCTTGCGGAATCGCTGGAACGCCAGGGGCAACTc < 1:2731301/68‑1 (MQ=255)
tGCAGGAAAGCGGTAGCCCTATCGATCTGATTACTCTTGCGGAATCGCTGGAACGCCAGGGGCAACTc < 1:1324618/68‑1 (MQ=255)
tGCAGGAAAGCGGTAGCCCTATCGATCTGATTACTCTTGCGGAATCGCTGGAACGCCAGGGGCAACTc < 1:2211413/68‑1 (MQ=255)
tGCAGGAAAGCGGTAGCCCTATCGATCTGATTACTCTTGCGGAATCGCTGGAACGCCAGGGGCAACTc < 1:1372530/68‑1 (MQ=255)
tGCAGGAAAGCGGTAGCCCTATCGATCTGATTACTCTTGCGGAATCGCTGGAACGCCAGGGGCAACTc < 1:3052348/68‑1 (MQ=255)
tGCAGGAAAGCGGTAGCCCTATCGATCTGATTACTCTTGCGGAATCGCTGGAACGCCAGGGGCAACTc < 1:2684050/68‑1 (MQ=255)
aGGAAAGCGGTAGCCCTATCGATCTGATTACTCTTGCGGAATCGCTGGAACGCCAGGGGCAACTCGAt < 1:2600957/68‑1 (MQ=255)
aGGAAAGCGGTAGCCCTATCGATCTGATTACTCTTGCGGAATCGCTGGAACGCCAGGGGCAACTCGAt < 1:2779217/68‑1 (MQ=255)
ggAAAGCGGTAGCCCTATCGATCTGATTACTCTTGCGGAATCGCTGGAACGCCAGGGGCAACTCGAt < 1:2605038/67‑1 (MQ=255)
ggTAGGCCTATCGATCTGATTACTCTTGCGGAATCGCTGGAACGCCAGGGGCAACTc < 1:1972487/57‑1 (MQ=255)
gTAGCCCTATCGATCTGATTACTCTTGCGGAATCGCTGGAACGCCAGGGGCAACTCGATAGCGTCggtg < 1:2689008/69‑1 (MQ=255)
gTAGCCCTATCGATCTGATTACTCTTGCGGAATCGCTGGAACGCCAGGGGCAACTCGATAGCGTCggtg < 1:3357789/69‑1 (MQ=255)
gTAGCCCTATCGATCTGATTACTCTTGCGGAATCGCTGGAACGCCAGGGGCAACTCGATAGCGTCggtg < 1:2524284/69‑1 (MQ=255)
gTAGCCCTATCGATCTGATTACTCTTGCGGAATCGCTGGAACGCCAGGGGCAACTCGATAGCGTCggtg < 1:37620/69‑1 (MQ=255)
gTAGCCCTATCGATCTGATTACTCTTGCGGAATCGCTGGAACGCCAGGGGCAACTCGATAGCGTCggtg < 1:1609581/69‑1 (MQ=255)
cTATCGATCTGATTACCCTTGCGGAATCGCTGGAACGCCa < 1:2316012/40‑1 (MQ=255)
tATCGATCTGATTACTCTTGCGGAATCGCTGGAACGCCAGGGGCa > 1:1488169/1‑45 (MQ=255)
tATCGATCTGATTACTCTTGCGGAATCGCTGGAACGCCAGGGGCAACTCGATAGCGTCGGTGGTTTTg > 1:2226948/1‑68 (MQ=255)
gATCTGATTACTCTTGCGGAATCGCTGGAACGCCAGGGGCAACTCGATAGCGTCGGTGGTTTTGCtt < 1:169927/67‑1 (MQ=255)
gATCTGATTACTCTTGCGGAATCGCTGGAACGCCAGGGGCAACTCGATAGCGTCGGTGGTTTTGCTTa < 1:2810452/68‑1 (MQ=255)
aCTCTTGCGGAATCGCTGGAACGCCAGGGGCAACTCGATAGCGTCGGTGGTTTTGCTTATCTGGCaga > 1:2871210/1‑68 (MQ=255)
|
CACACCGTCATATCTTTACTGAAATGGCGCGTTTGCAGGAAAGCGGTAGCCCTATCGATCTGATTACTCTTGCGGAATCGCTGGAACGCCAGGGGCAACTCGATAGCGTCGGTGGTTTTGCTTATCTGGCAGA > minE/2803378‑2803510
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
