Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,815,784 |
C→T |
49.3% |
G480G (GGC→GGT) |
yjcC → |
predicted signal transduction protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,815,784 | 0 | C | T | 49.3%
| ‑0.6
/ 66.3
| 75 | G480G (GGC→GGT) | yjcC | predicted signal transduction protein |
| Reads supporting (aligned to +/- strand): ref base C (14/24); new base T (20/17); total (34/41) |
| Fisher's exact test for biased strand distribution p-value = 1.67e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CATTTGATTGCGGAACACATCATCGAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAGGCGTCGAAACTGAGGAGCAGGTTAACTGGCTGCGCAAACGCGGCGTGCGCTATTGCCAGGGATGGTTCTT > minE/2815719‑2815852
|
cATTTGATTGCGGAACACATCATCGAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAGGCGt > 1:3273973/1‑68 (MQ=255)
ttGATTGCGGAACACATCATCGAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAGGTGTCGaa < 1:2663864/69‑1 (MQ=255)
ttGATTGCGGAACACATCATCGAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAGGTGTCGaa < 1:1696906/69‑1 (MQ=255)
ttGATTGCGGAACACATCATCGAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAGGTGTCGaa < 1:1726527/69‑1 (MQ=255)
ttGATTGCGGAACACATCATCGAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAGGTGTCGaa < 1:666303/69‑1 (MQ=255)
ttGATTGCGGAACACATCATCGAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAGGTGTCGaa < 1:3143016/69‑1 (MQ=255)
ttGATTGCGGAACACATCATCGAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAGGTGTCGaa < 1:1941330/69‑1 (MQ=255)
ttGATTGCGGAACACATCATCGAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAGGTGTCGaa < 1:31080/69‑1 (MQ=255)
ttGATTGCGGAACACATCATCGAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAGGTGTCGaa < 1:2040497/69‑1 (MQ=255)
ttGATTGCGGAACACATCATCGAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAGGTGTCGaa < 1:2049035/69‑1 (MQ=255)
ttGATTGCGGAACACATCATCGAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAGGTGTCGaa < 1:1409419/69‑1 (MQ=255)
ttGATTGCGGAACACATCATCGAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAGGTGTCGaa < 1:2857898/69‑1 (MQ=255)
ttGATTGCGGAACACATCATCGAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAGGTGTCGaa < 1:2520623/69‑1 (MQ=255)
ttGATTGCGGAACACATCATCGAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAGGTGTCGaa < 1:930662/69‑1 (MQ=255)
ttGATTGCGGAACACATCATCGAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAGGTGTCGaa < 1:2551853/69‑1 (MQ=255)
tGATTGCGGAACACATCATCGAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAGGTGTCGaa < 1:1981336/68‑1 (MQ=255)
tGATTGCGGAACACATCATCGAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAGGTGTCGaa < 1:3288983/68‑1 (MQ=255)
tGCTGAACACATCATCTAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAGGCGTCGAAACTg < 1:1037552/68‑1 (MQ=255)
tGCGGAACACATCATCGAGCTGGCGCACAGCCTGGGTTTAAAAACGATCGCTGAAGGCGTCGAAACTg < 1:2859344/68‑1 (MQ=255)
tGCGGAACACATCATCGAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAGGCGTCGAAACTg < 1:1140569/68‑1 (MQ=255)
tGCGGAACACATCATCGAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAGGCGTCGAAACTg < 1:1196574/68‑1 (MQ=255)
tGCGGAACACATCATCGAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAGGCGTCGAAACTg < 1:2494990/68‑1 (MQ=255)
tGCGGAACACATCATCGAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAGGCGTCGAAACTg < 1:3044452/68‑1 (MQ=255)
tGCGGAACACATCATCGAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAGGCGTCGAAACTg < 1:193281/68‑1 (MQ=255)
cacaTCATCGAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAGGCGTCGAAACTGAGGAGCAg < 1:1928155/69‑1 (MQ=255)
acaTCATCGAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAGGTGTCGaa < 1:1620744/56‑1 (MQ=255)
atcatcGAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAGGCGTCGAAACTGAGGAGCAGGtt < 1:2698648/69‑1 (MQ=255)
atcGAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAGGCGTCGAAACTGAGGAGCAGGTTAAc > 1:1727220/1‑69 (MQ=255)
atcGAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAGGCGTCGAAACTGAGGAGCAGGTTAAc > 1:2208279/1‑69 (MQ=255)
atcGAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAGGCGTCGAAACTGAGGAGCAGGTTAAc > 1:1523601/1‑69 (MQ=255)
atcGAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAGGCGTCGAAACTGAGGAGCAGGTTAAc > 1:3335191/1‑69 (MQ=255)
atcGAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAGGCGTCGAAACTGAGGAGCAGGTTAAc > 1:550321/1‑69 (MQ=255)
atcGACCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAGGCGTCGAAACTGAGGAGCAGGTTAAc > 1:246671/1‑69 (MQ=255)
cGAGCTGGCTCACAGCCTGGGGTTAAAAACGATCGCTGAAGGCGTCGAAACTGAGGAGCAGGTTAACTg < 1:2168687/69‑1 (MQ=255)
cGAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAGGCGTCGAAACTGAGGAGCAGGTTAACTg < 1:2551888/69‑1 (MQ=255)
ttAAAAACGATCGCTGAAGGCGTCGAAACTGAGGAGc > 1:1662630/1‑37 (MQ=255)
ttAAAAACGATCGCTGAAGGCGTCGAAACTGAGGAGc > 1:2258093/1‑37 (MQ=255)
ttAAAAACGATCGCAGAAGGCGTCGAAACTGAGGAGc > 1:2867529/1‑37 (MQ=255)
gtAAAAACGATCGCTGAAGGCGTCGAAACTGAGGAGc > 1:1734270/2‑37 (MQ=255)
aaaaaCGATCGCTGAAGGCGTCGAAACTGAGGAGCAGGTTAACTGGCTGCGCaaa > 1:1846805/1‑55 (MQ=255)
aaaaaCGATCGCTGAAGGCGTCGAAACTGAGGAGCAGGTTAACTGGCTGCGCaaa > 1:2199035/1‑55 (MQ=255)
aaaaaCGATCGCTGAAGGCGTCGAAACTGAGGAGCAGGTTAACTGGCTGCGCaaa > 1:3347940/1‑55 (MQ=255)
cGCTGAAGGTGTCGAAACTGAGGCGCAGGTTAACTGGCTg > 1:722934/1‑40 (MQ=255)
cGCTGAAGGTGTCGAAACTGAGGCGCAGGTTAACTGGCTGCGCAAACGCGGCGTGCGCTATTGCCAggg > 1:225881/1‑69 (MQ=255)
cGCTGAAGGTGTCGAAACTGAGGCGCAGGTTAACTGGCTGCGCAAACGCGGCGTGCGCTATTGCCAggg > 1:2723258/1‑69 (MQ=255)
cGCTGAAGGTGTCGAAACTGAGGCGCAGGTTAACTGGCTGCGCAAACGCGGCGTGCGCTATTGCCAggg > 1:2478563/1‑69 (MQ=255)
cGCTGAAGGTGTCGAAACTGAGGCGCAGGTTAACTGGCTGCGCAAACGCGGCGTGCGCTATTGCCAggg > 1:2822926/1‑69 (MQ=255)
cGCTGAAGGTGTCGAAACTGAGGCGCAGGTTAACTGGCTGCGCAAACGCGGCGTGCGCTATTGCCAggg > 1:2326052/1‑69 (MQ=255)
cGCTGAAGGTGTCGAAACTGAGGCGCAGGTTAACTGGCTGCGCAAACGCGGCGTGCGCTATTGCCAggg > 1:1285751/1‑69 (MQ=255)
cGCTGAAGGTGTCGAAACTGAGGCGCAGGTTAACTGGCTGCGCAAACGCGGCGTGCGCTATTGCCAggg > 1:1675548/1‑69 (MQ=255)
cGCTGAAGGTGTCGAAACTGAGGCGCAGGTTAACTGGCTGCGCAAACGCGGCGTGCGCTATTGCCAggg > 1:928102/1‑69 (MQ=255)
cGCTGAAGGTGTCGAAACTGAGGCGCAGGTTAACTGGCTGCGCAAACGCGGCGTGCGCTATTGCCAggg > 1:677366/1‑69 (MQ=255)
cGCTGAAGGTGTCGAAAATGAGGCGCAGGTTAACTGGCTGCGCAAACGCGGCGTGCGCTATTGCCAggg > 1:672050/1‑69 (MQ=255)
cGCTGAAGGTGTCGAAAATGAGGCGCAGGTTAACTGGCTGCGCAAACGCGGCGTGCGCTATTGCCAggg > 1:964802/1‑69 (MQ=255)
cGCTGAAGGTGTCGAAAATGAGGCGCAGGTTAACTGGCTGCGCAAACGCGGCGTGCGCTATTGCCAggg > 1:627946/1‑69 (MQ=255)
cGCTGAAGGTGTCGAAAATGAGGCGCAGGTTAACTGGCTGCGCAAACGCGGCGTGCGCTATTGCCAggg > 1:2344548/1‑69 (MQ=255)
cGCTGAAGGTGTCGAAAATGAGGCGCAGGTTAACTGGCTGCGCAAACGCGGCGTGCGCTATTGCCAggg > 1:1050603/1‑69 (MQ=255)
cGCTGAAGGTGTCGAAAATGAGGCGCAGGTTAACTGGCTGCGCAAACGCGGCGTGCGCTATTGCCAggg > 1:1596251/1‑69 (MQ=255)
cGCTGAAGGTGTCGAAAATGAGGCGCAGGTTAACTGGCTGCGCAAACGCGGCGTGCGCTATTGCCAggg > 1:1691230/1‑69 (MQ=255)
cGCTGAAGGTGTCGAAAATGAGGCGCAGGTTAACTGGCTGCGCAAACGCGGCGTGCGCTATTGCCAggg > 1:1792080/1‑69 (MQ=255)
cGCTGAAGGTGTCGAAAATGAGGCGCAGGTTAACTGGCTGCGCAAACGCGGCGTGCGCTATTGCCAggg > 1:1852595/1‑69 (MQ=255)
cGCTGAAGGTGTCGAAAATGAGGCGCAGGTTAACTGGCTGCGCAAACGCGGCGTGCGCTATTGCCAggg > 1:1948027/1‑69 (MQ=255)
cGCTGAAGGCGTCGAAACTGAGGAGCAGGTTAACTGGCTGCGCAAACGCGGCGTGCGCTATTGcc < 1:2068033/65‑1 (MQ=255)
cGCTGAAGGCGTCGAAACTGAGGAGCAGGTTAACTGGCTGCGCAAACGCGGCGTGCGCTATTGcc < 1:1924131/65‑1 (MQ=255)
cGCTGAAGGCGTCGAAACTGAGGAGCAGGTTAACTGGCTGCGCAAACGCGGCGTGCGCTATTGcc < 1:1840583/65‑1 (MQ=255)
cGCTGAAGGCGTCGAAACTGAGGAGCAGGTTAACTGGCTGCGCAAACGCGGCGTGCGCTATTGcc < 1:2105733/65‑1 (MQ=255)
cGCTGAAGGCGTCGAAACTGAGGAGCAGGTTAACTGGCTGCGCAAACGCGGCGTGCGCTATTGcc < 1:2175592/65‑1 (MQ=255)
cGCTGAAGGCGTCGAAACTGAGGAGCAGGTTAACTGGCTGCGCAAACGCGGCGTGCGCTATTGcc < 1:1640985/65‑1 (MQ=255)
cGCTGAAGGCGTCGAAACTGAGGAGCAGGTTAACTGGCTGCGCAAACGCGGCGTGCGCTATTGcc < 1:230089/65‑1 (MQ=255)
cGCTGAAGGCGTCGAAACTGAGGAGCAGGTTAACTGGCTGCGCAAACGCGGCGTGCGCTATTGcc < 1:1456224/65‑1 (MQ=255)
cGCTGAAGGCGTCGAAACTGAGGAGCAGGTTAACTGGCTGCGCAAACGCGGCGTGCGCTATTGcc < 1:1421126/65‑1 (MQ=255)
tGAAGGCGTCGAAACTGAGGAGCAGGTTAACTGGCTGCGCAAACGCGGCGTGCGCTATTGCCAGGGAt < 1:188424/68‑1 (MQ=255)
tGAAGGCGTCGAAACTGAGGAGCAGGTTAACTGGCTGCGCAAACGCGGCGTGCGCTATTGCCAGGGAt < 1:1503947/68‑1 (MQ=255)
tGAAGGCGTCGAAACTGAGGAGCAGGTTAACTGGCTGCGCAAACGCGGCGTGCGCTATTGCCAGGGAt < 1:1192364/68‑1 (MQ=255)
tGAAGGCGTCGAAACTGAGGAGCAGGTTAACTGGCTGCGCAAACGCGGCGTGCGCTATTGCCAGGGAt < 1:264305/68‑1 (MQ=255)
cGTCGAAACTGAGGAGCAGGTTAACTGGCTGCGCAAACGCGGCGTGCGCTATTGCCAGGGATGGTTCtt < 1:3035819/69‑1 (MQ=255)
cGTCGAAACTGAGGAGCAGGTTAACTGGCTGCGCAAACGCGGCGTGCGCTATTGCCAGGGATGGTTCtt < 1:1788538/69‑1 (MQ=255)
cGTCGAAACTGAGGAGCAGGTTAACTGGCTGCGCAAACGCGGCGTGCGCTATTGCCAGGGATGGTTCtt < 1:906366/69‑1 (MQ=255)
|
CATTTGATTGCGGAACACATCATCGAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAGGCGTCGAAACTGAGGAGCAGGTTAACTGGCTGCGCAAACGCGGCGTGCGCTATTGCCAGGGATGGTTCTT > minE/2815719‑2815852
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
