Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,835,378 |
G→T |
27.1% |
D173E (GAC→GAA) |
yjeJ ← |
hypothetical protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,835,378 | 0 | G | T | 27.1%
| 161.2
/ 52.2
| 103 | D173E (GAC→GAA) | yjeJ | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (46/29); new base T (18/10); total (64/39) |
| Fisher's exact test for biased strand distribution p-value = 8.23e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.73e-01 |
Only 100 of 103 total aligned reads displayed.
ATAATGGTCGAACAAGTTATGTTTCCATTCCGGTTGGGAATAGGTGTCATATTCCAGATTGCCATTGTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGTGAGGTAATACGTA > minE/2835312‑2835433
|
aTAATGGTTGAACAAGTTATGTTTCCATTCCGGTTGGGAATAGGTGTCATATTCCAGATTGCCATTTTc < 1:943987/69‑1 (MQ=255)
aTAATGGTTGAACAAGTTATGTTTCCATTCCGGTTGGGAATAGGTGTCATATTCCAGATTGCCATTTTc < 1:239975/69‑1 (MQ=255)
aTAATGGTTGAACAAGTTATGTTTCCATTCCGGTTGGGAATAGGTGTCATATTCCAGATTGCCATTTTc < 1:2403442/69‑1 (MQ=255)
aTAATGGTTGAACAAGTTATGTTTCCATTCCGGTTGGGAATAGGTGTCATATTCCAGATTGCCATTTTc < 1:2102636/69‑1 (MQ=255)
aTAATGGTTGAACAAGTTATGTTTCCATTCCGGTTGGGAATAGGTGTCATATTCCAGATTGCCATTTTc < 1:2602370/69‑1 (MQ=255)
aTAATGGTTGAACAAGTTATGTTTCCATTCCGGTTGGGAATAGGTGTCATATTCCAGATTGCCATTTTc < 1:811650/69‑1 (MQ=255)
aTAATGGTTGAACAAGTTATGTTTCCATTCCGGTTGGGAATAGGTGTCATATTCCAGATTGCCATTTTc < 1:695182/69‑1 (MQ=255)
aTAATGGTTGAACAAGTTATGTTTCCATTCCGGTTGGGAATAGGTGTCATATTCCAGATTGCCATTTTc < 1:3185181/69‑1 (MQ=255)
aTAATGGTTGAACAAGTTATGTTTCCATTCCGGTTGGGAATAGGTGTCATATTCCAGATTGCCATTTTc < 1:326800/69‑1 (MQ=255)
ggTCGAACAAGTTATGTTTCCATTCCGGTTGGGAATAGGTGTCATATTCCAGATTGCCATTGTCCTgg > 1:2414724/1‑68 (MQ=255)
ggTCGAACAAGTTATGTTTCCATTCCGGTTGGGAATAGGTGTCATATTCCAGATTGCCATTGTCCTGGc > 1:2282596/1‑69 (MQ=255)
ggTCGAACAAGTTATGTTTCCATTCCGGTTGGGAATAGGTGTCATATTCCAGATTGCCATTGTCCTGGc > 1:2176215/1‑69 (MQ=255)
ggTCGAACAAGTTATGTTTCCATTCCGGTTGGGAATAGGTGTCATATTCCAGATTGCCATTGTCCTGGc > 1:1479428/1‑69 (MQ=255)
ggTCGAACAAGTTATGTTTCCATTCCGGTTGGGAATAGGTGTCATATTCCAGATTGCCATTGTCCTGGc > 1:514136/1‑69 (MQ=255)
ggTCGAACAAGTTATGTTTCCATTCCGGTTGGGAATAGGTGTCATATTCCAGATTGCCATTGTCCTGGc > 1:926802/1‑69 (MQ=255)
ggTCGAACAAGTTATGTTTCCATTCCGGTTGGGAATAGGTGTCATATTCCAGATTGCCATTGTCCTGGc > 1:1203576/1‑69 (MQ=255)
gTCGAACAAGTTATGTTTCCATTCCGGTTGGGAATAGGTGTCATATTCCAGATTGCCATTGTCCTGGCa > 1:14785/1‑69 (MQ=255)
gTCGAACAAGTTATGTTTCCATTCCGGTTGGGAATAGGTGTCATATTCCAGATTGCCATTGTCCTGGCa > 1:217313/1‑69 (MQ=255)
gTCGAACAAGTTATGTTTCCATTCCGGTTGGGAATAGGTGTCATATTCCAGATTGCCATTGTCCTGGCa > 1:885669/1‑69 (MQ=255)
gTCGAACAAGTTATGTTTCCATTCCGGTTGGGAATAGGTGTCATATTCCAGATTGCCATTGTCCTGGCa > 1:2461169/1‑69 (MQ=255)
gTCGAACAAGTTATGTTTCCATTCCGGTTGGGAATAGGTGTCATATTCCAGATTGCCATTGTCCTGGCa > 1:2832063/1‑69 (MQ=255)
gTCGAACAAGTTATGTTTCCATTCCGGTTGGGAATAGGTGTCATATTCCAGATTGCCATTGTCCTGGCa > 1:2022104/1‑69 (MQ=255)
gTCGAACAAGTTATGTTTCCATTCCGGTTGGGAATAGGTGTCATATTCCAGATTGCCATTGTCCTGGCa > 1:2018241/1‑69 (MQ=255)
gTCGAACAAGTTATGTTTCCATTCCGGTTGGGAATAGGTGTCATATTCCAGATTGCCATTGTCCTGGCa > 1:1972166/1‑69 (MQ=255)
gTCGAACAAGTTATGTTTCCATTCCGGTTGGGAATAGGTGTCATATTCCAGATTGCCATTGTCCTGGCa > 1:63110/1‑69 (MQ=255)
gTCGAACAAGTTATGTTTCCATTCCGGTTGGGAATAGGTGTCATATTCCAGATTGCCATTGTCCTGGCa > 1:1783532/1‑69 (MQ=255)
gTCGAACAAGTTATGTTTCCATTCCGGTTGGGAATAGGTGTCATATTCCAGATTGCCATTGTCCTGGCa > 1:3327888/1‑69 (MQ=255)
gTCGAACAAGTTATGTTTCCATTCCGGTTGGGAATAGGTGTCATATTCCAGATTGCCATTGTCCTGGCa > 1:1704479/1‑69 (MQ=255)
gTCGAACAAGTTATGTTTCCATTCCGGTTGGGAATAGGTGTCATATTCCAGATTGCCATTGTCCTGGCa > 1:1580593/1‑69 (MQ=255)
gTCGAACAAGTTATGTTTCCATTCCGGTTGGGAATAGGTGTCATATTCCAGATTGCCATTGTCCTGGCa > 1:554845/1‑69 (MQ=255)
cAAGTTATTTTTCCATTCCGGTTGGGAATAGGTGTCATATTCCAGATTGCCATTGTCCTGGCAGTCAAc > 1:848613/1‑69 (MQ=255)
cAAGTTATTTTTCCATTCCGGTTGGGAATAGGTGTCATATTCCAGATTGCCATTGTCCTGGCAGTCAAc > 1:852563/1‑69 (MQ=255)
cAAGTTATGTTTCCATTCCGGTTGGGAATAGGTGTCATATTCCAGATTGCCATTGTCCTGGCAGTCAAc > 1:684434/1‑69 (MQ=255)
cAAGTTATGTTTCCATTCCGGTTGGGAATAGGTGTCATATTCCAGATTGCCATTGTCCTGGCAGTCAAc > 1:912912/1‑69 (MQ=255)
tGGGAATAGGTGTCATATTCCAGATTGCCATTGTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCt > 1:2705246/1‑69 (MQ=255)
tGGGAATAGGTGTCATATTCCAGATTGCCATTGTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCt < 1:401348/69‑1 (MQ=255)
tGGGAATAGGTGTCATATTCCAGATTGCCATTGTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCt < 1:471271/69‑1 (MQ=255)
tGGGAATAGGTGTCATATTCCAGATTGCCATTGTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCt > 1:593122/1‑69 (MQ=255)
tGGGAATAGGTGTCATATTCCAGATTGCCATTGTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCt < 1:1054045/69‑1 (MQ=255)
tGGGAATAGGTGTCATATTCCAGATTGCCATTGTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCt > 1:1595553/1‑69 (MQ=255)
tGGGAATAGGTGTCATATTCCAGATTGCCATTGTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCt > 1:125501/1‑69 (MQ=255)
tGGGAATAGGTGTCATATTCCAGATTGCCATTGTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCt < 1:21953/69‑1 (MQ=255)
tGGGAATAGGTGTCATATTCCAGATTGCCATTGTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCt > 1:1221436/1‑69 (MQ=255)
tGGGAATAGGTGTCATATTCCAGATTGCCATTGTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCt < 1:1220870/69‑1 (MQ=255)
tGGGAATAGGTGTCATATTCCAGATTGCCATTGTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCt > 1:2151330/1‑69 (MQ=255)
tGGGAATAGGTGTCATATTCCAGATTGCCATTGTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCt < 1:2071258/69‑1 (MQ=255)
gAATAGGTGTCATATTCCAGATTGCCATTGTCCTGGCTGTCAACGTCGTATAACGGCAGGAAATCTaa < 1:1018928/68‑1 (MQ=255)
gAATAGGTGTCATATTCCAGATTGCCATTGTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTaa < 1:2426318/68‑1 (MQ=255)
gAATAGGTGTCATATTCCAGATTGCCATTGTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTaa < 1:3036144/68‑1 (MQ=255)
gAATAGGTGTCATATTCCAGATTGCCATTGTCCTGGCAGTCAAAGTCGTATAACGGCAGGAAATCTaa < 1:3250382/68‑1 (MQ=255)
aaTAGGTGTCATATTCCAGATTGCCATTGTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACa > 1:3316982/1‑69 (MQ=255)
tAGGTGTCATATTCCAGATTGCCATTGTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGt < 1:1261732/69‑1 (MQ=255)
aGGTGTCATATTCCAGATTGCCATTGTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGTg < 1:1371073/69‑1 (MQ=255)
aGGTGTCATATTCCAGATTGCCATTGTCCTGGCAGTCAACGTCGTATAACGGAAGGAAATCTAACAGTg < 1:681283/69‑1 (MQ=255)
ggTGTCATATTCCAGATTGCCATTGTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGt < 1:2708764/67‑1 (MQ=255)
ggTGTCATATTCCAGATTGCCATTGTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGTGa > 1:413612/1‑69 (MQ=255)
ggTGTCATATTCCAGATTGCCATTGTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGTGa > 1:251211/1‑69 (MQ=255)
ggTGTCATATTCCAGATTGCCATTGTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGTGa > 1:778683/1‑69 (MQ=255)
ggTGTCATATTCCAGATTGCCATTGTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGTGa > 1:1217554/1‑69 (MQ=255)
tgtCATATTCCAGATTGCCATTGTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGTGAg > 1:2520159/1‑68 (MQ=255)
tCATATTCCAGATTGCCATTGTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGTGAGGt > 1:808358/1‑68 (MQ=255)
tCATATTCCAGATTGATATTGTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGTGAGGt > 1:2146162/1‑68 (MQ=255)
ttCCAGATTGCCATTGTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGTGAGGTAATAc > 1:1210889/1‑68 (MQ=255)
ttCCAGATTGCCATTGTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGTGAGGTAATAc > 1:1539953/1‑68 (MQ=255)
ttCCAGATTGCCATTGTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGTGAGGTAATAc > 1:1463365/1‑68 (MQ=255)
ttCCAGATTGCCATTGTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGTGAGGTAATAc > 1:675693/1‑68 (MQ=255)
ttCCAGATTGCCATTGTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGTGAGGTAATAc > 1:3092080/1‑68 (MQ=255)
ttCCAGATTGCCATTGTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGTGAGGTAATAc > 1:2291767/1‑68 (MQ=255)
ttCCAGATTGCCATTGTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGTGAGGTAATACg < 1:804933/69‑1 (MQ=255)
ttCCAGATTGCCATTGTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGTGAGGTAATACg < 1:2663060/69‑1 (MQ=255)
ttCCAGATTGCCATTGTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGTGAGGTAATACg < 1:2548610/69‑1 (MQ=255)
ccAGATTGCCATTTTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGTGAGGTAATAc > 1:1124398/1‑66 (MQ=255)
ccAGATTGCCATTTTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGTGAGGTAATACGTa > 1:1430872/1‑69 (MQ=255)
ccAGATTGCCATTTTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGTGAGGTAATACGTa > 1:557455/1‑69 (MQ=255)
ccAGATTGCCATTTTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGTGAGGTAATACGTa > 1:1460789/1‑69 (MQ=255)
ccAGATTGCCATTTTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGTGAGGTAATACGTa > 1:1964835/1‑69 (MQ=255)
ccAGATTGCCATTTTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGTGAGGTAATACGTa > 1:3158710/1‑69 (MQ=255)
ccAGATTGCCATTTTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGTGAGGTAATACGTa > 1:2064221/1‑69 (MQ=255)
ccAGATTGCCATTTTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGTGAGGTAATACGTa > 1:2766911/1‑69 (MQ=255)
ccAGATTGCCATTTTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGTGAGGTAATACGTa > 1:2061479/1‑69 (MQ=255)
ccAGATTGCCATTTTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGTGAGGTAATACGTa > 1:2847701/1‑69 (MQ=255)
ccAGATTGCCATTTTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGTGAGGTAATACGTa > 1:2065506/1‑69 (MQ=255)
ccAGATTGCCATTTTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGTGAGGTAATACGTa > 1:2087081/1‑69 (MQ=255)
ccAGATTGCCATTTTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGTGAGGTAATACGTa > 1:311098/1‑69 (MQ=255)
ccAGATTGCCATTTTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGTGAGGTAATACGTa > 1:1986801/1‑69 (MQ=255)
ccAGATTGCCATTTTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGTGAGGTAATACGTa > 1:1759287/1‑69 (MQ=255)
ccAGATTGCCATTTTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGTGAGGTAATACGTa > 1:3413816/1‑69 (MQ=255)
ccAGATTGCCATTTTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGTGAGGTAATACGTa > 1:355577/1‑69 (MQ=255)
ccAGATTGCCATTGTCCTGGCAGTCAACGTCGTATAACGTCAGGAAATCTAACAGTGAGGTAATACGt < 1:2307435/68‑1 (MQ=255)
ccAGATTGCCATTGTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGTGAGGTAATACGt < 1:2269104/68‑1 (MQ=255)
ccAGATTGCCATTGTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGTGAGGTAATACGt < 1:855583/68‑1 (MQ=255)
ccAGATTGCCATTGTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGTGAGGTAATACGt < 1:2269441/68‑1 (MQ=255)
ccAGATTGCCATTGTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGTGAGGTAATACGt < 1:376217/68‑1 (MQ=255)
ccAGATTGCCATTGTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGTGAGGTAATACGt < 1:2662324/68‑1 (MQ=255)
ccAGATTGCCATTGTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGTGAGGTAATACGt < 1:758316/68‑1 (MQ=255)
ccAGATTGCCATTGTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGTGAGGTAATACGt < 1:303153/68‑1 (MQ=255)
ccAGATTGCCATTGTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGTGAGGTAATACGt < 1:143420/68‑1 (MQ=255)
ccAGATTGCCATTGTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGTGAGGTAATACGt < 1:224680/68‑1 (MQ=255)
ccAGATTGCCATTGTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGTGAGGTAATACGt < 1:554729/68‑1 (MQ=255)
ccAGATTGCCATTGTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGTGAGGTAAAACGt < 1:3008921/68‑1 (MQ=255)
|
ATAATGGTCGAACAAGTTATGTTTCCATTCCGGTTGGGAATAGGTGTCATATTCCAGATTGCCATTGTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGTGAGGTAATACGTA > minE/2835312‑2835433
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
